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All Journal International Journal of Public Health Science (IJPHS) HAYATI Journal of Biosciences Jurnal Veteriner Jurnal Berkala Epidemiologi Indonesian Journal of Tropical and Infectious Disease Biology, Medicine, & Natural Product Chemistry Majalah Obstetri dan Ginekologi Dental Journal (Majalah Kedokteran Gigi) BIOMA Jurnal Biologi Tropis Jurnal Respirasi (JR) Majalah Biomorfologi (Biomorphology Journal) Narra J Berkala Penelitian Hayati Journal of Clinical Microbiology and Infectious Diseases Prosiding Seminar Nasional COSMIC Kedokteran Strada Jurnal Ilmiah Kesehatan (SJIK) Folia Medica Indonesiana
Kuntaman Kuntaman
Faculty Of Medicine, Universitas Wijaya Kusuma Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Veterinary

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The Correlation between icaA and icaD Genes with Biofilm Formation Staphylococcus epidermidis In Vitro Rachmawati, Dian; Kuntaman, Kuntaman; Alimsardjono, Lindawati
Folia Medica Indonesiana Vol. 55, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

This study was conducted to identify the presence of icaA and icaD genes in S. epidermidis and to analyze the relationship between the presence of icaA and icaD genes with the ability of in vitro biofilm formation in S. epidermidis. S. epidermidis isolates from patients and healthy people were collected and PCR was examined to detect icaA and icaD genes. which then continued to examine the ability of biofilm formation by the method of Congo Red Agar. The results of this genotypic and phenotypic examination were then tested for correlation with statistical tests using SPSS 23.0. A total of 40 S. epidermidis isolates were collected, consisting of 20 clinical isolates and 20 isolates of normal flora. The icaA gene was positive in 5 isolates (12.5%), and 8 isolates (20%) were positive for the icaD gene, 3 isolates with icaA and icaD were both positive. One hundred percent of isolates with icaA or icaD positively formed biofilms, but there were 15 isolates (42.9%) who did not have the icaA gene but showed the ability to form biofilms, while 12 isolates (37.5%) who did not have the icaD gene also formed biofilms. Fifty percent of S. epidermidis isolates showed the ability to form biofilms at CRA. The Fisher Exact test showed a significant relationship between the icaA gene and the ability of biofilm formation (p=0.047 (p<0.05)) as well as the icaD gene (p=0.03 (p<0.05)). The icaA and icaD genes have a significant relationship to biofilm formation in S. epidermidis. There was another mechanism in the formation of biofilms that are not dependent on the ica gene.
Comparation of Phenotypic and Genotypic Profile of Carbapenemase Producing Escherichia coli Sutandhio, Silvia; Budiono, Budiono; Hardiono, Hardiono; Kuntaman, Kuntaman; Wasito, Eddy Bagus; Lusida, Maria Inge
Folia Medica Indonesiana Vol. 54, No. 1
Publisher : Folia Medica Indonesiana

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Carbapenemase-producing Escherichia coli (E. coli) has caused trouble in therapeutic antibiotic selection. Carbapenemase screening procedure in laboratories is usually based on inacurate semi-automatic system. Confirmation and classification of carbapenemases according to Ambler can be done with combination of phenotypic methods, i.e., Modified Hodge Test (MHT), Sodium Mercaptoacetic Acid (SMA), and 3-Aminophenylboronic Acid (PBA). This study aimed to compare profiles of carbapenemase-producing E. coli which were confirmed and classified phenotypically with the genotypic profiles. E. coli isolates from urine specimens which were potential as carbapenemase-producers according to semi-automatic system BD Phoenix were phenotypically tested with MHT, SMA, and PBA. Isolates were grouped as carbapenemase-producers and non carbapenemase-producers. Phenotypic carbapenemase-producer isolates were classified based on Ambler criteria. All isolates were then tested with Polymerase Chain Reaction (PCR) for the presence of OXA-48, IMP1, IMP2, GES, VIM, NDM, KPC genes. Out of 30 isolates, 6 isolates (20.0%) were MHT positive, and 25 isolates (83.3%) were SMA positive, which indicated that most isolates produced were carbapenemase Ambler B. PCR confirmed 12 isolates (40.0%) had VIM gene which were classified as carbapenemase Ambler B. Phenotypic confirmatory test had 100% sensitivity and 22.2% specificity. Classification with phenotypic confirmatory test had 91.7% match with PCR. Phenotypic confirmatory test detected more carbapenemase than PCR. This low specificity may be caused by inappropriate use of diagnostic gold standard. PCR should not be used for routine carbapenemase confirmation because of vast diversity of carbapenemases. Phenotypic confirmatory test can classify carbapenemase according to Ambler classification.
The Prevalence of Extended Spectrum Beta-Lactamase (ESBL) Producing Gut Bacterial Flora Among Patients in Dr. Soetomo Hospital and Primary Health Centre in Surabaya Naelasari, Dian Neni; Koendhori, Eko Budi; Dewanti, Linda; Sarassari, Rosantia; Kuntaman, K
Folia Medica Indonesiana Vol. 54, No. 4
Publisher : Folia Medica Indonesiana

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The extended-spectrum b-lactamase (ESBL) producer bacteria until now were mostly identified in hospital environment. The aim of this study was to analyze the prevalence of ESBL-producing gut flora and distribution of ESBL encoding genes between hospitalized patient in Tropical Wards of Dr. Soetomo Hospital and patient from a primary health centre (PHC) as community environment in Surabaya. Thiry rectal swab samples from hospital of Dr. Soetomo patients and from PHC (60 samples in total) were collected for this study. Samples were screened in MacConkey agar supplemented with 2 mg/L of cefotaxim, incubated at 37ºC for 24 hours. Then the growing colony were confirmed with Disk Diffusion Synergy test (DDST) for diagnosis of ESBL producer. The identified ESBL producers were then identified the bacteria species by biochemical method. ESBL gene were detected by PCR with specific primers. The results showed that there was not difference of positif nuber of ESBL-producing bacteria gut floral between patients of Dr.Soetomo Hospital, 25/30 (83.3%) and PHC, 11/30 (36.7%) (p=1). The pattern of ESBL gene distributions among samples from hospital showed that SHV was 12%, TEM was 36%, and CTX-M was 80%, and from PHC were SHV 18.2%, TEM 27,3% and CTX-M 81,8%. Statistical analysis showed that the pattern was not significantly different among hospitals and PHC samples as shown by SHV gene (p=0,631), TEM (p= 0.715), and CTX -M (p=1). From each ESBL gene, the dominant genes that found producing ESBL were the CTX-M genes followed by TEM and SHV genes. The prevalence of ESBL producersin intestinal flora of both the hospital (83,3%) and the PHC (36,7%) was very high. There was not significant difference between the prevalence of ESBL producer in gut flora of hospitalized patients compared to PHC. There was found other patterns of ESBL gene combinations in the hospital of SHV+CTX-M genes, TEM+CTX-M, SHV+TEM+CTX-M genes and PHC, the combination pattern of SHV+CTX-M, TEM+CTX-M.
Phylogenetic and Transmission Patterns of Extended-Spectrum β- Lactamase –Producing Escherichia Coli among Neonatal's Gut Flora in Indonesia Sulistya, Rakhmatul Binti; Kuntaman, Kuntaman; Koendhori, Eko Budi; Happy, Terza Aflika; Setyarini, Wahyu
Folia Medica Indonesiana Vol. 57, No. 3
Publisher : Folia Medica Indonesiana

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This study aimed to explore the phylogenetic pattern of Extended-spectrum β-lactamase-producing Escherichia coli in the gut flora of neonatal into groups A, B1, B2, D and tracing the presence of spreading among the age group of neonatal. Multiplex PCR was conducted to classify the phylogenetic group of ESBL producing E. coli into groups of commensals (A, B1) and pathogen (B2, D). The spread of bacterial agents among the host (neonatal) was conducted by the RAPD-PCR method with two random primers. Among 34 bacterial isolates were identified among early neonatal group A (22,2%), B1 (11,1%), B2 (22,2%) D (33,3%) and postneonatal groups A (8%), B1 (4%), B2 (36%) D (40%). It was not a statistically significant difference (p=0.388). The dominance of pathogenic groups B2 and D as many as 70,6%. From 34 samples was found 34 types of RAPD indicated there were not any bacterial spread among neonatal. The ESBL producing E. coli among early neonatal and postneonatal were dominated by the pathogenic group of B2 and D (total=70,6%), but they were not statistically significant. There was not any spreading of bacterial agents among individual gut flora of neonatal.
Prevalence Pattern of Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli in the Intestinal Flora of Milkfish Salsabillah, Dinara Safina; Kuntaman, Kuntaman; Masfufatun, Masfufatun; Tania, Putu Oky Ari
Jurnal Biologi Tropis Vol. 26 No. 1 (2026): Januari-Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i1.11259

Abstract

Escherichia coli is one of the gram-negative bacteria that produces the Extended Spectrum Beta-Lactamase (ESBL) enzyme and has a role in resistance to the beta-lactam group. The presence of E. coli bacteria that produce ESBL in food, especially in fish such as milkfish (Chanos chanos) poses a potential source of the spread of antimicrobial resistance (AMR) in humans. This study aims to determine the prevalence of ESBL-producing E. coli in the intestinal flora of milkfish sold in traditional markets in Surabaya City. This study used a descriptive observational method with a cross-sectional approach. There were 50 samples of milkfish intestines taken from several stalls in traditional markets randomly. Identification of E. coli bacteria by planting in MacConkey media with 4 µL/ml cefotaxime followed by the Double Disk Synergy Test (DDST). Of the 50 milkfish intestine samples used, 34 (68%) samples showed positive results for ESBL-producing E. coli. The high prevalence may indicate that the potential for the spread of antimicrobial resistance through the food chain, food hygiene management and controlling the use of antibiotics in fish farming need serious attention.
Co-Authors A. M. Widya A.A.W., I Wayan Putra Abdul Khairul Rizki Purba Mustofa Indwiani Astuti AFAF BAKTIR Agung Dewi Sekar Agus Santosa Agus Santosa akhmad sudibya, akhmad Alimsardjono, Lindawati Aquaresta, Febriana Ardani, I Gusti Wahju Ardhiya Puspita Ardi Eko Marsanto Arina Setyaningtyas Aryati , Aryati Aryati Aryati Aryati Aryati Bahraini, Fahrisah Nurfadeliah Benediktus Yohan Binti Mu’arofah Bramantono Bramantono, Bramantono Budi Prasetyo Budi Utomo Budiono Budiono Budiono Budiono Deby Kusumaningrum Dewi, Lisa Dharmawati, Ira Dian Neni Naelasari Dian Rachmawati Diani Dwi Indrasari Didik Hasmono Dwiyanti Puspitasari, Dwiyanti Eddy Bagus Wasito Eko B. Koendhori Eko Budi Khoendori Eko Budi Koendhori, Eko Budi Erni J. Nelwan, Erni J. Erni Juwita Nelwan, Erni Juwita Fourier Dzar Eljabbar Latief Gondo Mastutik Happy, Terza Aflika Hardiono Hardiono Hardiono Hardiono, Hardiono Hari Basuki Notobroto Hari Paraton Hari Paraton Hidayah, Ana Nurlaili Ida Bagus Narmada Iqhrammullah, Muhammad Ira Widjiastuti Irwanto Irwanto Ishak Samuel Wuwuti Kartuti Debora, Kartuti Kusbaryanto L. Alimsardjono Latief Mooduto, Latief Linda Dewanti Listyawati, Agusniar Furkani Ma'rifah, Nurul Maftuchah Maftuchah Margyaningsih, Nur Ita Maria Inge Lusida Maria Inge Lusida Marinda Dwi Puspitarini Mariyatul Qiptiyah, Mariyatul Marsheila Harvy Mustikaningtyas Masfufatun Masfufatun Masyeni, Sri Muchlis AU Sofro N. D. Kurniati Naritha Noor Cholies Zaini Nur Dianawati Nurul Ma'rifah P. N. Endraputra Parawidnyaningsih, Putu Ayu Aryanda Pristiawan Navy Endraputra Purnomo, Windu Puspitarini, Marinda Dwi Putu Arya Suryanditha Putu Oky Ari Tania R. Tedjo Sasmono Radita Yuniar Arizandy Rakhmatul Binti Sulistya Rasita, Yoeke Dewi Regita Cahyani, Nafansya Rini Devijanti Ridwan Risnanda, Kadek Rio Rosantia Sarassari Rosantia Sarassari Rosantia Sarassari Rosantia Sarassari S. R. S. Oktaviani S. Soedarsono Sacharissa Zerlina Tsarwah Thirafi Salsabillah, Dinara Safina Sarassari, Rosantia Sasmono, R. Tedjo Semedi, Bambang Pujo Setyarini, Wahyu Sholeh, Mohammad Akbaruddin Silvia Sutandhio Soekoyo, Agusta Reny Sofro, Muchlis AU Sofro, Muchlis AU. Sri Masyeni, Dewa Ayu Putri Sulistiawati Sulistiawati Sulistya, Rakhmatul Binti Syaiful, Irbasmantini Tamara Yuanita Terza Aflika Happy Thayyib, Muqoddar Triffit Imasari UNTUNG MURDIYATMO Usman Hadi Wahyu Setyarini Wahyu Setyorini Wahyu, Agung Dwi Wahyuni Dyah Parmasari, Wahyuni Dyah Windu Purnomo Wiwiek Tyasningsih WJ. Pudjirahardjo Yasmeen Lashari Yoeke Dewi Rasita Yohan, Benediktus