Article Per Year (5 Year)
p-Index From 2020 - 2025
4.827
P-Index
This Author published in this journals
All Journal ODONTO Dental Journal Majalah Obat Tradisional Dentino: Jurnal Kedokteran Gigi Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Journal of Indonesian Dental Association Jurnal Kreativitas PKM Dentin Jurnal Insan Farmasi Indonesia Odonto dental journal
Tri Putri, Deby Kania
Department Of Biomedic, Faculty Of Dentistry, Universitas Lambung Mangkurat, Banjarmasin
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Physics Public Health

Published : 43 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Dental Journal (Majalah Kedokteran Gigi)

 1 
Characterization of Streptococcus sanguis molecular receptors for Streptococcus mutans binding molecules Deby Kania Tri Putri; Indah Listiana Kriswandini; Muhammad Luthfi
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 4 (2016): December 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.91 KB) | DOI: 10.20473/j.djmkg.v49.i4.p213-216

Abstract

Background: Dental caries is a major problem in oral cavity. If dental caries causes cavity, the structure of dental hard tissue will not be reversible because of damage in the structure of the hard tissue. The early pathogenesis mechanism of dental caries is an adhesion interaction between cariogenic Streptococcus mutans microorganisms and tooth surface pellicles. The attachment involves a specific molecular component interaction between the bacterial complement molecules and the surface of the host. Streptococcus sanguis as a dominant ecology at the beginning of bacterial plaque aggregation will colonize the tooth surface earlier than S. mutans. The surface of bacterial cells can express some adesin. The bacteria also can express receptors for adhesins of other bacteria. Specific receptors for adhesions of S. Mutans bacteria are not only found in the pellicles, but also present in pioneer bacteria, such as S. sanguis. Adhesion between those bacteria is called as coagregation. Purpose: This study aimed to analyze the characterization of Streptococcus sanguis molecular receptors for Streptococcus mutans binding molecules. Method: This study used a sonication method for protein isolation of S. mutans and S. sanguis bacterial biofilms, as well as electrophoresis method using 12 % SDS-PAGE gel and Western Blot analysis. Result: Results of the protein profile analysis of S. mutans biofilms using 12% SDS-PAGE showed that there were 17 bands, each of which molecular weights was 212, 140, 81, 65, 61, 48, 45, 44, 40, 39, 33 , 25, 23, 19, 17, 12, and 11 kDa. On the other hand, results of the protein profile analysis of S. sanguis biofilms using 12% SDS-PAGE showed that there were 15 bands, each of which molecular weight was 130, 85, 65, 61, 48, 46, 40, 37, 29, 25, 23, 21, 17, 15, and 12 kDa. And, results of the analysis of S. sanguis receptor molecules using Western blot showed that there were three bands, each of which molecular weight was 130, 85, and 40 kDa. Conclusion: S. sanguis bacteria have specific receptor molecules for S. mutans bacteria with the molecular weight of 130, 85, and 40 kDa.
Antioxidant activity test of ethyl acetate fraction of binjai (Mangifera Caesia) leaf ethanol extract K. Khairiah; Irham Taufiqurrahman; Deby Kania Tri Putri
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 4 (2018): December 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.714 KB) | DOI: 10.20473/j.djmkg.v51.i4.p164-168

Abstract

Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxidant properties which promote wound healing inhibiting oxidation radicals. The natural antioxidants present in binjai leaves can be extracted by fractionation. Purpose: This study aimed to analyze the antioxidant activity of ethyl acetate fraction in 96% ethanol extract of binjai leaf. Methods: The study constituted a pure experimental study incorporating a post-test design with only random sampling technique consisting of two groups, namely; an ethyl acetate fraction as the treatment group and ascorbic acid as the positive control group. The leaves were treated in accordance with the soxhlet method and subsequently fractionated to extract ethyl acetate fraction. This was used to measure antioxidant activity with DPPH radical damping method using a UV-Vis spectrophotometer. A linear regression calculation was performed with a standard curve to quantify the IC50 value, before the ethyl acetate fraction underwent a qualitative test of secondary metabolite. Results: An independent t-test indicated significant differences between groups, an average value of IC50 in ascorbic acid of 13.812 ppm with 0.996 linearity and a fraction of ethyl acetate 38.526 ppm with a linearity of 0.999. In contrast, at this linearity value ascorbic acid and ethyl fraction acetate demonstrate a very high linear connection between concentration and inhibition. A secondary metabolite test conducted on the ethyl acetate fraction produced positive results for flavonoid, tannins, and phenol. Conclusion: Based on the IC50 parameters, the fraction of ethyl acetate in 96% ethanol extract of binjai leaf produces very strong antioxidant activity in the content of the compounds in the fraction, namely: flavonoid, tannins and phenol.
Co-Authors Agung Satria Wardhana Aizar Agi Syahrial Aizar Agi Syahrial, Aizar Agi Amalia Putri, Amalia Amelia, Aura Amy Nindia Carabelly Annisa Rahma Ariyati Retno Pratiwi, Ariyati Retno Aspriyanto, Didit Aulia Azizah Aulia Rahimah Azilita Ananda Azwar Fida Maulana Bambang Setiawan Bayu Indra Sukmana Bayu Yordha Senggara Beta Widya Oktiani Budipramana, Melissa Candra Candra Dewi Nurdiana Dewi Retno Wahyu Widyaningrum Diana Wibowo Duhan Kanzu Balad Eny Febriyanti Erika Norfitriah Fariz Asyraq, Muhammad Hafly Fatimah Maulideya Fatma Kirana Ferdy Juliannor Fajar Firdaus, I Wayan Arya Krishnawan Fitriyana, Annisa Haliza, Ninda Andrea Hamdani, Riky Hatta, Isnur Hutami, Wijayanti Diah Wasi Ichrom Nahzi, Muhammad Yanuar Ika Kusuma Wardani Indah Listiana Kriswandini Jamilatun Nisa Juli Harnida Purwaningayu K. Khairiah Kamila, Syifa Maharani Laillyza Apriasari Maulana, Noval Ihza Melati Ainun Nisa Melisa Budipramana, Melisa Moch. Andhika Firdausi Rachfa Muhammad Alfi Ghifari Muhammad Irfan Fanshuri Muhammad Luthfi Muhammad Luthfi MUHAMMAD RAYHAN Muhammad Zainal Fikri Nadalia Malika Bilqis Nadiya Amalia Naila Khairiyah Nurrahman, Tri Nurul Fitriyani Dewi Oktiani, Beta Widya Priyawan Rahmadi Rahma Dania A.J Rahmadella, Afifah Rahmadi, Priyawan Renie Kumala Dewi Robiyansyah, Hengki Rosihan Adhani, Rosihan Sarifah, Norlaila Senggara, Bayu Yordha Setyawardhana, Raden Harry Dharmawan Sherli Diana Sitepu, Alexander Siti Khairiah Siti Raudah Siti Rohmah Tomy Wira Wahyuda Utami, Juliyatin Putri Vena Paramita Djunaidy Wardhana, Agung Satria Wydiamala, Erida Yuniar Elsa Dwinuria Yusrinie Wasiaturrahmah Zahra, Aisyah Nur