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All Journal Darussalam Nutrition Journal Celebes Biodiversitas : Jurnal Sains dan Pendidikan Biologi Jurnal SainHealth Bioma : Jurnal Biologi dan Pembelajaran Biologi Jurnal Analis Medika Biosains (JAMBS) Jurnal Biomedika Medical Technology and Public Health Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Medical and Health Science Journal Community Development Journal Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Abdimas Madani dan Lestari (JAMALI) Amalee: Indonesian Journal of Community Research & Engagement Community Development Journal: Jurnal Pengabdian Masyarakat BERNAS: Jurnal Pengabdian Kepada Masyarakat Medicra (Journal of Medical Laboratory Science/Technology) Indonesia Berdaya Jurnal Pengabdian Masyarakat Indonesia Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Environmental and Toxicology Management (ETM) Journal of Health Community Service Warmadewa Minesterium Medical Journal SWARNA Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Journal of Artificial Intelligence and Digital Business Jurnal Penelitian dan Kajian Ilmiah Kesehatan Politeknik Medica Farma Husada Mataram National Conference for Ummah BIOSAPPHIRE: Jurnal Biologi dan Diversitas SWARNA: Jurnal Pengabdian Kepada Masyarakat al Kimiya : Jurnal Ilmu Kimia dan Terapan BIOTROPIC
Prayekti, Endah
Universitas Nahdlatul Ulama Surabaya
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Pengujian Stabilitas Antibakteri Terhadap Staphylococcus aureus Dari Eksrak Etanol Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr) Dengan Perlakuan Suhu Penyimpanan Prayekti, Endah; Prayoga, Mirshall Abiedama
BIOSAPPHIRE: Jurnal Biologi dan Diversitas Vol. 1 No. 2 (2022): BIOSAPPHIRE
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, UNIPAR JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biosapphire.v1i2.690

Abstract

Bawang dayak merupakan salah satu tanaman yang biasanya digunakan sebagai obat tradisional di daerah Kalimantan. Bawang dayak diketahui mengandungan bebebrapa senayawa fitokimia seperti alkaloid, tanin, flavonoid dan glikosida yang dapat digunakan sebagai antibakteri. Penelitian ini dilakukan secara in-vitro dengan teknik kertas cakram (Kirby- Bauer) dengan menguji ekstrak umbi bawang dayak terhadap salah satu faktor fisik yaitu suhu penyimpanan. Pengujian kemampuan antibakteri dilakukan pada Staphylococcus aureus. Konsentrasi ekstrak bawang dayak yang digunakan dalam penelitian ini sebesar 40%. Suhu penyimpanan yang digunakan dalam penelitian ini adalah 4oC, 20oC, dan 40oC . Hasil penelitian menunjukkan bahwa pada suhu 4oC didapatkan diameter zona hambat dengan rata-rata 25,33 mm, pada suhu 20oC didapatkan diameter zona hambat dengan rata-rata 32,5 mm dan pada suhu 40oC didapatkan diameter zona hambat dengan rata-rata 29,83 mm. Hasil uji Kruskal Wallis didapatkan nilai signifikansi 0,00 yang berarti suhu berpengaruh terhadap stabilitas ekstrak etanol bawang dayak dalam menghambat pertumbuhan Staphylococcus aureus. Suhu penyimpanan yang paling optimal untuk menyimpan ekstrak umbi bawang dayak adalah pada suhu 20oC.
Improving Public and Environmental Health Through Rheumatoid Screening and Education on Environment Pathogenic Fungal Contamination Maat, Suprapto; Prayekti, Endah; Nugraha, Gilang; Ramadani, Vania Nur; Melindah, Winda; Mukarramah, Imraatul
Indonesia Berdaya Vol 7, No 1 (2026)
Publisher : UKInstitute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47679/ib.20261411

Abstract

People working in agricultural environments with high humidity levels are at greater risk of exposure to pathogenic fungi, which can trigger infections of the skin, nails, and respiratory tract, especially in individuals with weakened immune systems. On the other hand, the elderly are vulnerable to health decline, including autoimmune diseases such as rheumatoid arthritis (RA), which can cause joint inflammation, tissue damage, and progressive disability. This community service activity aims to increase public knowledge about the dangers of pathogenic fungal contamination and to conduct early detection of RA through Rheumatoid Factor (RF) testing. Education was delivered interactively using lectures, discussions, and visual media, accompanied by pre- and post-tests, as well as RF testing using the latex agglutination method. This community service activity was attended by 33 participants. Results showed an increase in knowledge from 58% to 91% after the education, with all 33 RF test participants receiving non-reactive results. This program demonstrates that the combination of education and simple testing can increase public preventive awareness and is suitable for adaptation in other areas with similar risk characteristics.
Improving Public and Environmental Health Through Clean and Healthy Living Behavior Education and Bacterial Examination Prayekti, Endah; Santoso, Andreas Putro Ragil; Sunaryo, Merry; Indaryanti, Reski Novita; Rahmawati, Lili Susan; Kamelia, Dina
Indonesia Berdaya Vol 7, No 1 (2026)
Publisher : UKInstitute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47679/ib.20261400

Abstract

A major problem frequently faced by the public is clean and healthy living behaviors (PHBS). According to the Indonesian Ministry of Health in 2014, health can be achieved by changing unhealthy behaviors into healthy ones and creating a health-supportive environment. Effective measures include handwashing with soap and environmental inspections. This activity aims to increase public awareness of the importance of clean and healthy living behavior (PHBS), while also introducing the dangers of microbiological contamination that can occur on hands and water. Community service activities are carried out through the stages of pre-test, education, post-test, sampling, and sample examination. Of the 34 respondents, the majority experienced a significant reduction in hand bacteria, with 21 showing a reduction in hand bacteria of more than 70% and some reaching 99–100%. Well water examinations at several public facilities for Pekingan village residents showed the presence of Coliform bacteria exceeding the safe limits according to the Ministry of Health Regulation, indicating that the water is not suitable for direct consumption due to possible contamination by domestic waste, septic tank seepage, or poor environmental sanitation. The conclusion of this Community Service activity is that there is an increase in respondents' understanding and an increase in their ability to practice proper handwashing. Water sanitation in public facilities needs to be considered and improved because it should not be used for consumption.
Perbandingan Penggunaan Buffer CTAB dan Surfaktan ABS Untuk Isolasi DNA Candida albicans Althofur Hilman Rifki; Endah Prayekti
Biotropic : The Journal of Tropical Biology Vol. 9 No. 1 (2025): Biotropic, Volume 9 Nomor 1, 2025
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v9i1.2179

Abstract

This study aimed to compare the effectiveness of CTAB buffer and Alkyl Benzene Sulfonate (ABS) surfactant in isolating DNA from Candida albicans. The fungi were cultured in Sabouraud Dextrose Broth (SDB) for 3 days at 37°C, and the DNA isolation process was carried out through three stages: cell lysis, precipitation, and purification. The results of DNA isolation were evaluated qualitatively using electrophoresis and quantitatively using a Nanodrop Spectrophotometer. The results showed that DNA isolation was successful using Candida albicans CTAB buffer and Alkyl Benzene Sulfonate (ABS) surfactant. This was proven by visualization of genomic DNA bands from electrophoresis results and nanodrop spectrophotometer counts. The highest DNA concentration was found when using CTAB buffer with an average concentration of 65.5 ng/µl. Meanwhile, in ABS surfactant, the highest DNA concentration was found at a concentration of 12%, amounting to 31.3 ng/µl. DNA purity when using CTAB buffer and Alkyl Benzene Sulfonate (ABS) surfactant with a concentration of 12% resulted in pure results. in CTAB buffer the average DNA purity was 1.88. At a concentration of 12% Alkyl Benzene Sulfonate (ABS) surfactant, the average DNA purity was 1.81. From the results obtained, the Alkyl Benzene Sulfonate (ABS) surfactant cannot completely replace the CTAB buffer for Candida albicans DNA isolation. Alkyl Benzene Sulfonate (ABS) surfactant can be used as an alternative to CTAB buffer to obtain good DNA purity, but the concentration cannot match that of CTAB buffer.
In Silico Detection of Antimicrobial Resistance and Virulence Genes in Methicillin Resistant Staphylococcus aureus Clinical Isolates: A Comparative Genomics Approach Muhammad Taufiq Hidayat; Endah Prayekti; Muhammad Afwan Romdloni; Seggaf Achmad Syah
BIOPENDIX Vol 13 No 1 (2026): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol13issue1page7-13

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) remains a formidable threat in both community and healthcare settings, thanks to its ability to evade β-lactam antibiotics and accumulate resistance to multiple drug classes. Here, we sequenced and compared the genomes of 13 recent clinical MRSA isolates alongside two well-characterized reference strains (N315 and NCTC 8325). By applying the ResFinder and VirulenceFinder pipelines, we rapidly cataloged each strain’s antibiotic resistance and virulence repertoire. Every MRSA isolate carried the hallmark mecA gene, and most also harbored blaZ, which encodes penicillinase. Resistance determinants for aminoglycosides (aac(6′)-Ie-aph(2″), aph(3′)-III), macrolides (erm(C), mph(C)), and chloramphenicol (cat variants) appeared in various combinations across the collection. On the virulence side, genes for α- and γ-hemolysins (hla, hlgABC) were universal, and nearly all strains possessed phage-encoded immune-evasion factors (sak, scn). The total count of virulence genes ranged from ten to fourteen per genome, peaking in two particularly gene-rich isolates. Our findings highlight the genetic diversity of MRSA, where multidrug resistance and a broad toxin arsenal coexist. Moreover, this study underscores the speed and reliability of in silico screening tools for antimicrobial-resistance surveillance and comparative genomics. Future work should integrate laboratory assays and patient data to link these genomic profiles to clinical outcomes.
The Detection of the Escherichia coli Gene in Well Water in Bojonegoro Using Polymerase Chain Reaction (PCR) Prayekti, Endah; Rosiana Dewi Regita; Khiliah Navis
Biotropic : The Journal of Tropical Biology Vol. 10 No. 1 (2026): Biotropic, Volume 10 Nomor 1, 2026
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v10i1.2449

Abstract

ABSTRACT Escherichia coli (E. coli) is an indicator of water contamination that can be pathogenic, especially the Enterotoxigenic E. coli (ETEC) pathotype, which carries the heat-resistant enterotoxin (est) gene. Well water that does not meet clean water quality standards can become a medium for Escherichia coli contamination, so it is important to perform molecular genetic detection. The study aimed to determine the presence of Escherichia coli bacteria and detect the est gene from well water isolates using the Polymerase Chain Reaction (PCR) method. Samples were collected from 19 wells using purposive sampling techniques. Escherichia coli identification was performed through conventional isolation, Gram staining, biochemical tests, and culture on selective media (EMB and MCA). The results of this study showed that four samples tested positive for Escherichia coli. Molecular detection using PCR for the est gene in Escherichia coli was not detected in any of the four Escherichia coli isolates. Based on these results, it was concluded that Escherichia coli was present in the samples, but the est gene was not detected. Keywords: Escherichia coli, est gene, well water, PCR ABSTRAK Escherichia coli (E. coli) merupakan indikator pencemaran air yang dapat bersifat patogen, terutama patotipe Enterotoxigenic E. coli (ETEC) yang membawa gen enterotoksin tahan panas (est). Air sumur yang tidak memenuhi standar kualitas air bersih dapat menjadi media kontaminasi Escherichia coli, sehingga penting dilakukan deteksi genetik secara molekuler. Penelitian bertujuan untuk mengetahui keberadaan bakteri Escherichia coli dan mendeteksi gen est dari isolat air sumur menggunakan metode Polymerase Chain Reaction (PCR). Sampel diambil dari 19 sumur menggunakan teknik purposive sampling. Identifikasi Escherichia coli dilakukan melalui isolasi konvensional, pewarnaan Gram, uji biokimia, dan kultur pada media selektif (EMB dan MCA). Hasil penelitian ini menunjukkan adanaya 4 sampel yang memiliki positif Escherichia coli. Deteksi molekuler dengan PCR terhadap gen est pada Escherichia coli tidak ditemukan pada keempat isolat Escherichia coli. Berdasarkan hasil penelitian ini disimpulkan bahwa pada sampel ditemukan Escherichia coli tetapi tidak ditemukan gen est. Kata Kunci: Escherichia coli, gen est, air sumur, PCR
Edukasi Bahaya Penyalahgunaan Antibiotik dan Pemaanfaatan Tanaman Herbal untuk Pengobatan Endah Prayekti; Nathalya Dwi Kartika Sari; Ary Andini; Renny Novi Puspitasari; Yauwan Tobing Lukiyono; Khiliah Navis
Warmadewa Minesterium Medical Journal Vol. 2 No. 2 (2023): Mei 2023
Publisher : Warmadewa University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/wmmj.2.2.2023.73-79

Abstract

Antibiotics are generally known in the community as a medicine for disease. The use of antibiotics in society is often not matched by knowledge of the consequences of misuse of antibiotics. One of the most dangerous effects of uncontrolled use of antibiotics in humans, animals, or animal products is the presence of bacterial resistance to antibiotics. The existence of bacterial resistance to antibiotics causes infected patients to take longer to recover. The purpose of this community service activity is to provide education to Tanjung Gresik Village Health cadres on the correct use of antibiotics and provide insight into the use of herbal plants as natural medicines. The method of implementing community service is carried out using the seminar method and followed by the distribution of booklets and flyers for reading material for village cadres. After implementation, village cadres are required to fill out an understanding evaluation form for the material that has been provided. The results of the initial measurement indicated that 33 village cadres had knowledge regarding antibiotics, but only an average of 13 participants were able to answer the initial questions correctly. Initial measurements related to herbal plants for treatment, of the 15 herbal plants that were informed, 10 herbal plants had known benefits. While the other 5 herbal plants have not been widely known for their benefits for treatment. After the activity, the understanding of cadres has increased. Increased understanding of the use of antibiotics reached 25% for 16 participants, 50% for 3 participants, and 75% for 1 participant. While the use of herbal plants increased by 5-30% for 9 participants, > 20-30% for 5 participants and > 50% for 5 participants. Based on the results obtained from this activity, village cadres can use the information obtained to be conveyed to residents.
CYTOTOXICITY ASSAY USING BRINE SHRIMP LETHALITY TEST ON COLLAGEN-CHITOSAN WOND DRESSING STERILIZED BY ULTRAVIOLET LIGHT Andini, Ary; Prayekti, Endah; Dyah Wulandari, Devyana; Nidianti, Ersalina
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i1.1467

Abstract

Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer as known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible materials. Combination of collagen and chitosan expected to be the best biomaterials as a wound dressing for the healing process. The study aimed to determine cytotoxicity assay on collagen-chitosan wound dressing sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained collagen 75%-Chitosan 25%. Collagen extracted from skin and scalp of snakehead fish (Channa striata) then mixed with chitosan until formed collagen-chitosan wound dressing. This study used Brine Shrimp Level Test (BSLT) method with solution concentration 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results showed that K0, K1, K2, and K3 group had LC50 >1000 that proven collagen-chitosan wound dressing was non-toxic materials. As conclusion of the study explained composite wound dressing based on collagen-chitosan in all groups that sterilized under UV-Light along 15 minutes was not toxic based on Brine Shrimp Lethality Test showed LC50 >1000.
PURPLE SWEET POTATO (Ipomoea batatas L.) PEELS EXTRACT AS AN ALTERNATIVE DYE FOR BACTERIA GRAM STAINING Nunki, Nastasya; Titik Mutiarawati, Diah; Prayekti, Endah
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i2.1655

Abstract

Crystal violet and Safranin are dyes in Gram staining, which are carcinogenic. Alternative safe materials are needed to minimize the use of carcinogenic properties. Purple sweet potato (Ipomoea batatas L.) peels were the candidate of the alternative dye source because of its high anthocyanin pigment. The purpose of this study was to determine purple sweet potato (Ipomoea batatas L.) peels extract as an alternative to Gentian violet in Gram staining of bacteria. Extracts obtained from purple sweet potato peels studied with varying concentrations of 50%, 60%, and 75% for 1, 3, and 5 min as a substitute for Gentian violet on Bacillus sp. The parameters observed from this study based on visual field clarity, glass slide cleanliness, contrast, bacterial shape, bacterial colour. Each extract concentration compared with a control group using Gentian violet. The results showed that optimum staining in 50% concentration for 5 min, 60% concentration for 5 min, 75% concentration for 3 min, and 5 min respectively. The present study exhibited the potency of Ipomoea batatas L. peels extract as an alternative staining agent.
Comparation Between Mac conkey and Coconut Water Medium as a Growth Medium for Escherichia coli Prayekti, Endah; Suliati, Suliati; Wulandari, Dwi Agustin
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1906

Abstract

Escherichia coli is the bacteria that can cause diarrhea in humans and often used as a parameter of stool environmental pollution. Culture of E. coli from the sample often requires Mac Conkey as commercial media which is able to distinguish it from other bacteria in the Enterobacteriaceae group. Commercial media such as Mac Conkey certainly has a price that is quite expensive because of its ability as a growth medium for Enterobacteriaceae. Therefore, in the study tested natural ingredients that can be used for growth media, such as coconut water. The purpose of this study was to compare the ability of Mac Conkey media and coconut water to support the growth of E. coli. This research is an experimental study with a completely randomized design. The concentration of coconut water tested was 0%, 20%, 40%, 60%, 80%, and 100%. The results showed that at the concentration of coconut water 20% to 60% the number of E. coli colonies on coconut water media was slightly below the Mac Conkey Agar media, while in coconut water a concentration of 80% showed a greater number of colonies than Mac Conkey. The Mann Whitney test showed a significant difference between the number of colonies on 80% coconut water media and Mac Conkey Agar, which was equal to 0.004 (p < 0.05). Based on these results, coconut water has the potential to be used as a growth medium for E. coli.
Co-Authors Akbar Reza Muhammad Akhmad, Nur Faradila Althofur Hilman Rifki Andini, Ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Ardyarini Dyah Savitri Arsya Tazkiya Asma' Denaya Psari Yuwono Awaliyah, Arisma Putri Baihaqi, Muhammad Azhari Brina Thursina Dibiasi Devita Rahma Putri Devyana Dyah Wulandari dr Hartatiek Nila,Sp.OG Elsya Octaviani Cantika Putri Endah Budi Permana Putri Ersalina Nidianti Eva Rosa Dwi Febrianti Evi Sylvia Awwalia Fadillah Triasmoro Fakurazi, Sharida Gilang Nugraha Gilang Nugraha Hamiduumajid Ballihg Ballihgoo Hamiduumajid Ballihg Ballihgoo Hidayat, Muhammad Taufiq Husein Firdaus Ibrahim Dwi Waluyo Putra Imraatul Mukarramah Indah Nur Kamaliyah Indaryanti, Reski Novita Junaedy, M Dwinanda Kamelia, Dina Kardina, Rizki Nurmalya Khiliah Navis Khiliah Navis Khusnah, Hidayatul Kifli, Nurolaini Lili Susan Rahmawati Luqiana Fauziah M Nasir Maat, Suprapto Maki Zamzam Mardhotillah, Rachma Rizqina Marinda Dwi Puspitarini Melindah, Winda Mia Puspitasari Mirshall Abiedama Prayoga Mochamad Faishal Riza Muhammad Afwan Romdloni Muhammad Taufiq Hidayat Muhammad Taufiq Hidayat Mukarramah, Imraatul Mutiarawati, Diah Titik Nada Mufarrohah Nandasari, Anggy Dwipa Nathalya Dwi Kartika Sari Navis, Khiliah Ngiluhtara Aditiya Putri Nisa, Fauziyatun Nunki, Nastasya Nur Naili Fahira Nur Sophia Matin Pratama, Nizar Revandini Prayoga, Mirshall Abiedama Prisnidiawati, Ajeng Puspitasari, Renny Novi Putri Lestari, Ajeng Anindhita Putri, Ananda Ghufron Yuana Putri, Nasya Desinta Qonita Iklila Maghfuroh Qotrinnada, Nabilah Rahmawati, Lili Susan Ramadani, Vania Nur Regina Ayu Fristiyanti Rhomadhoni , Muslikha Nourma Riza, Mochammad Faishal Rizki Jalil, Ike Wasilatur Rizki Nurmalya Kardina Romdhoni, Muhammad Afwan Rosiana Dewi Regita Sa'bania Hari Raharjeng Sabrina, Shinta Milenia Sadza, Lailatus Salfa Salsabilah Zain Salimi, Syafrina Maulidyatus Salman Firmansyah Sari, Devi Fitriana Satya Nugraha Wirayudha Seggaf Achmad Syah Septina Isro’ Hermawati Setiarsih, Dini Setyana Yuli, Azizah Ari Siti Husnul Khotimah Sofaria Munir, Ratna Sonia Fitriatul Qolbi Suliati Suliati Sunaryo, Merry Suryandaru, Suryandaru Syafiuddin, Achmad Tauziah, Amalia Thomas Sumarsono Thomas Sumarsono Thomas Sumarsono Tullah, Auria Hidayah Uliyatul Laili, Uliyatul Umaha, Farida Wandara Sekar Ayu Pramesti Wideistianti, Annisa Pricilya Widya Novita Sari Wiwik Winarningsih Wulan, Wieke Sri Wulandari, Dwi Agustin Yauwan Tobing Lukiyono Yuliana Yuliana Yuni Nur Malita Sari Zahrotul Illiyin Zain, R.A Wafiqah Aulia Zalfa, Anisatuz Zazabilla, Indira