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Effectiveness of the repellent of kawista leaf extract (Limonia acidissima) against Aedes aegypti mosquitoes Qotrinnada, Nabilah; Lukiyono, Yauwan Tobing; Prayekti, Endah; Nidianti, Ersalina
Environmental and Toxicology Management Vol. 3 No. 2 (2023): Environmental drivers of vector-borne: Impact and mitigation
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/etm.v3i2.6390

Abstract

Synthetic insecticides containing highly effective active substances such as DEET (N,N-diethyl-meta toluamide) to prevent and control Dengue Hemorrhagic Fever can have adverse effects on health and the environment. Kawista leaves (Limonia acidissima) have the potential as a natural insecticide against Aedes aegypti mosquitoes because they contain secondary metabolite compounds namely phenols, flavonoids, saponins, and terpenoids. This study aims to determine the effectiveness of the repellent power of kawista leaf extract (Limonia acidissima) against Aedes aegypti mosquitoes. This study used experimental method with post-test only control group design. The sample used was 25 adult of Aedes aegypti mosquitoes in each treatment. The ethanol extract of kawista leaves was made in the form of a spray with a concentration of 25%, 50% concentration, and 75% concentration. The 25% concentration, 50% concentration, and 75% concentration of kawista leaf extract have an effective repellent power against Aedes aegypti mosquitoes, namely 15.22%, 46.20%, and 70.08%, respectively. The results of the analysis of kawista leaf extract against Aedes aegypti mosquitoes obtained ED50 (Effective Dose 50) at a concentration of 60.343% and ED99 (Effective Dose 99) at a concentration of 124.452%. From the results of the study, it can be concluded that kawista leaves have the ability to repel Aedes aegypti mosquitoes.
Relationship between personal hygiene and manifestation of mite (Sarcoptes scabiei) in students at islamic boarding school in Sidoarjo City Wideistianti, Annisa Pricilya; Lukiyono, Yauwan Tobing; Andini, Ary; Prayekti, Endah
Environmental and Toxicology Management Vol. 3 No. 2 (2023): Environmental drivers of vector-borne: Impact and mitigation
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/etm.v3i2.6391

Abstract

Scabies is a disease that attacks the skin in humans, a disease caused by the mite, included in the ovoid Arthropod organism. Personal hygiene is a way to maintain health and cleanliness of the body with self-care, therefore the better a person maintains personal hygiene, the less exposure to mites that enter the skin, if someone does not maintain personal hygiene, it will be very easy to get scabies. This study aims to determine the relationship between personal hygiene and the manifestation of mites using the burrow ink test method on students at the Islamic Boarding School in Sidoarjo City. The method used is an observational type that is analytical using a cross-sectional design. A sample of 45 samples in March 2024. The sampling method was carried out using a non-probability sampling method. The samples were examined using the burrow ink test method and continued with skin scraping examination. The results of the examination of 45 samples showed that there were 9 respondents in the good category of personal hygiene clothing cleanliness with a positive scabies examination of 27 (60%) and there were 25 respondents in the poor category of personal hygiene clothing cleanliness with a negative scabies examination of 41 (91%). The results of statistical analysis from the chi-square test, the personal hygiene variable with the manifestation of mites produced a p-value = 0.157 which means there is no significant effect. In this study it can be concluded that there is no relationship between personal hygiene using the burrow ink test method and the incidence of scabies in Islamic Boarding Schools in Sidoarjo City.
Comparison of the insidence of toxoplasmosis in feature samples of house cats and market cats in Sidoarjo City Zazabilla, Indira; Lukiyono, Yauwan Tobing; Santoso, Andreas Putro Ragil; Prayekti, Endah
Environmental and Toxicology Management Vol. 3 No. 3 (2023): Soil Health, Animal Health, and Water Quality in Agroecological Systems
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/etm.v3i3.6459

Abstract

To determine the comparison of toxoplasmosis in the feces of cats house and cats market in the Sidoarjo City. This method is a direct slide method using a direct preparation using a 2% Eosin solution. The principle of stool examination uses a direct method that uses a microscope to determine stool that is positive for Toxoplasmosis. The results of this research were obtained in the form of primary data, namely the results of a microscope examination. There were 16 fecal samples taken by purposive sampling in the Sidoarjo City. This research was conducted at the Parasitology Laboratory at Universitas Nahdlatul Ulama Surabaya, Surabaya. Cats are animals that are very popular as pets. Cats can transmit zoonotic diseases, namely toxoplasma, which is caused by the Toxoplasmosis parasite. Toxoplasmosis infection is an intracellular parasite from the group of protozoa which is an obligate parasite, where cats act as definitive hosts. Results on the incidence of Toxoplasmosis using feces samples from cats house and cats market found no Toxoplasmosis infection.
PERBEDAAN HASIL KADAR HEMOGLOBIN MENGGUNAKAN HEMATOLOGY ANALYZER DAN POCT Zalfa, Anisatuz; Nugraha, Gilang; Wulan, Wieke Sri; Prayekti, Endah
Jurnal Penelitian dan Kajian Ilmiah Kesehatan Politeknik Medica Farma Husada Mataram Vol. 10 No. 2 (2024): Oktober
Publisher : Politeknik Medica Farma Husada Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33651/jpkik.v10i2.548

Abstract

Hemoglobin is a protein molecule in red blood cells that carries O2 throughout the body. Hemoglobin contains iron which makes blood red. The hemoglobin level is a measure of the respiratory pigment present in the erythrocytes, which is used to determine anemia. This study was to see if there were differences in the results of hemoglobin levels using a hematology analyzer and POCT. This study used a hematology analyzer and POCT to determine the accuracy of the results obtained on the examination of hemoglobin levels. The aim of this study was to determine differences in the results of hemoglobin levels using a hematology analyzer and POCT. This type of research is analytic observational with a cross sectional approach. This study used venous blood samples using the hematology analyzer method and capillary blood samples using the POCT method as many as 54 samples. Collecting data using purposive sampling technique. The research was conducted in May 2023. The analytical test used the Independent T Test. Based on the research results, the p-value = 0.212 so that H0 is accepted, which means that there is no difference in the results of hemoglobin levels using a hematology analyzer and POCT. Based on the independent t test, the results obtained were a p-value of 0.212, which means that there was no difference in the results of hemoglobin levels using a hematology analyzer and POCT.
ANALISIS KANDUNGAN LEMAK TIDAK JENUH PADA KUE SEMPRONG BERBASIS CAMPURAN TEPUNG BIJI BUNGA MATAHARI DAN QUINOA Raharjeng, Sa'bania Hari; Kardina, Rizki Nurmalya; Prayekti, Endah; Tullah, Auria Hidayah; Sabrina, Shinta Milenia
Darussalam Nutrition Journal Vol. 8 No. 2 (2024): Darussalam Nutrition Journal
Publisher : Universitas Darussalam Gontor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/dnj.v8i2.11409

Abstract

Latar belakang: Angka kejadian penyakit tidak menular semakin meningkat dan berpotensi mengarah pada permasalahan kesehatan serta komplikasi penyakit lainnya. Kebiasaan konsumsi camilan dari bahan makanan tinggi lemak jenuh menjadi salah satu penyebab terjadinya permasalahan tersebut. Kebutuhan akan camilan yang lebih sehat dengan kandungan lemak tidak jenuh perlu menjadi perhatian. Tujuan: Penelitian ini bertujuan untuk menganalisis perbedaan kandungan zat gizi Polyunsaturated Fatty Acids (PUFA) dan Monounsaturated Fatty Acids (MUFA) pada kue semprong berbasis tepung biji bunga matahari dan tepung quinoa. Metode: Penelitian ini merupakan penelitian eksperimental dengan perlakuan berupa perbedaan komposisi tepung biji bunga matahari dan tepung quinoa dengan rasio Formula 1 (100:0), Formula 2 (75:25), dan Formula 3 (0:100). Hasil uji kandungan zat gizi dianalisis menggunakan uji one-way ANOVA. Hasil: Penggunaan tepung biji bunga matahari dan tepung quinoa sebagai pengganti tepung beras pada kue semprong menunjukkan hasil perbedaan kandungan Polyunsaturated Fatty Acids (PUFA) dan Monounsaturated Fatty Acids (MUFA) yang signifikan antar kelompok perlakuan (p<0,05). Formula 1 rata-rata mengandung 20,48g PUFA dan 9,62g MUFA; Formula 2 rata-rata mengandung 14,86g PUFA dan 8,27g MUFA; Formula 3 rata-rata mengandung 3,17g PUFA dan 4,27g MUFA.  Simpulan: Formula terbaik ada pada Formula 1 yang mengandung PUFA dan MUFA paling tinggi. Sedangkan formula 3 mengandung PUFA dan MUFA paling rendah. Semakin tinggi kandungan tepung biji bunga matahari dalam formula, maka kandungan PUFA dan MUFA akan semakin tinggi. Kata Kunci : biji bunga matahari, kue semprong, monounsaturated fatty acids, polyunsaturated fatty acids, quinoa
Detection of mecA Genes from Staphylococcus aureus Bacteria in Raw Cow Milk in the Surabaya Regional Farm Putri Lestari, Ajeng Anindhita; Prayekti, Endah Prayekti; Hidayat, Muhammad Taufiq
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.416

Abstract

Milk is a very important food ingredient in meeting nutritional needs and a good growth medium for microorganisms. Staphylococcus aureus bacteria are pathogens that often contaminate milk. Staphylococcus aureus bacteria can cause a disease called mastitis. The acropolis is very susceptible to infections, treatment and prevention need to be done to avoid mastitis. Treatment of mastitis until now still uses antibiotics, improper use of antibiotics can cause Staphylococcus aureus resistance to antibiotics. The mecA gene is the gene responsible for beta-lactam antibiotic resistance. This study aims to determine the presence of the mecA gene in Staphylococcus aureus isolate from raw cow's milk. The samples from this study are 24 samples taken from dairy farms in the Surabaya Region. Isolation of Staphylococcus aureus bacteria by conventional microbiology based on culture properties, Gram staining and biochemical tests. The results of this study showed that there were 5 samples that were positive for Staphylococcus aureus. Molecular detection by PCR of the mecA gene in Staphylococcus aureus was not found in the five Staphylococcus aureus isolates. Based on this study has the result that the sample found Staphylococcus aureus but no mecA gene was found.
Pemberdayaan Masyarakat: Upaya Peningkatan Kompetensi Praktisi Kesehatan dalam Analisis Molekuler untuk Diagnosis dan Penatalaksanaan Penyakit: Indonesia Andini, Ary; Nugraha, Gilang; Hidayat, Muhammad Taufiq; Prayekti, Endah; Salimi, Syafrina Maulidyatus; Navis, Khiliah; Sari, Widya Novita; Fakurazi, Sharida
Jurnal Abdimas Madani dan Lestari (JAMALI) Volume 07, Issue 01, Maret 2025
Publisher : UII

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jamali.vol7.iss1.art5

Abstract

Molecular analysis has played a pivotal role in diagnosing and managing diseases during pandemics by enabling early outbreak detection and rapid identification of disease causes, leading to prompt treatment. However, there is still a pressing need to enhance the understanding and proficiency of molecular diagnostics in Southeast Asia. Therefore, proactive measures should be taken to improve comprehension through seminars and training programs focused on molecular analysis. The objective is to strengthen the diagnostic and management capabilities of healthcare workers concerning infectious diseases in Southeast Asia, ultimately aiming to enhance the overall health of the population. The target participants include healthcare professionals, medical laboratory technicians, educators, and students. Through collaboration with Universiti Putra Malaysia, two significant events were organized. The International Seminar on Medical Laboratory Technology (ISoMLatech), took place on July 5, 2023, with the theme "Advancements in Molecular Assay for Diagnosing and Managing Infectious Diseases in Southeast Asia." This event was held in a hybrid format, utilizing Zoom and the UNUSA Auditorium, and it attracted 510. Afterwards, a Short Course was conducted since August 13 to 15, 2023, at Universiti Putra Malaysia (UPM) with 22 participants, The materials received were as followed: the fundamentals of molecular examinations, immunochemical detection, epidemiology of infectious diseases, antimicrobial resistance, and visits to UPM Campus and Institute of Bioscience, UPM. The evaluation results indicate that over 75% of participants rated both ISoMLatech and Short Course as good or very good. Consequently, there is strong anticipation for the continued success and implementation of the program.
Stimulation of Creative Entrepreneurship: Utilizing Natural Materials in Healthcare through a Nanotechnology Approach Andini, Ary; Prayekti, Endah; Wulandari, Devyana Dyah; Romdloni , Muhammad Afwan; Syafiuddin, Achmad; Sari, Widya Novita; Zain, R.A Wafiqah Aulia; Akhmad, Nur Faradila; Suryandaru, Suryandaru; Kifli, Nurolaini
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 1 (2025)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

As the world becomes increasingly modern, technology continues to advance at a rapid pace. The rise of Artificial Intelligence (AI) has contributed to a decline in job opportunities, leading to an increase in unemployment rates. In response, the D-VI Health Analyst Study Program at UNUSA aims to nurture young entrepreneurs in the health sector by organizing seminars and training sessions on creative entrepreneurship. These focus on the utilization of natural materials in health services through a nanotechnology approach, targeting young people, medical laboratory technicians, lecturers, and students. The event featured speakers from industry and international experts to expand the knowledge of youth and the broader community. It was conducted online as part of the International Seminar on Medical Laboratory Technology (ISoMLatech), held on March 6, 2024, via Zoom, with 216 internal participants. Evaluation results showed that over 75% of participants rated the ISoMLatech event as good or very good. It is hoped that such activities will continue in the future, as they play a crucial role in enhancing the knowledge and skills of healthcare workers in advancing nanotechnology innovation within the global healthcare industry. Keyword: Nanotechnology, Natural product, Entrepreuner, Healtcare
Business Feasibility Analysis Herbaldrink NU NanoFit: Peluang dan Tantangan di Pasar Minuman Kesehatan Romdloni, Muhmmad Afwan; Andini, Ary; Mardhotillah, Rachma Rizqina; Khusnah, Hidayatul; Putri, Endah Budi Permana; Santoso, Andreas Putro Ragil; Nidianti, Ersalina; Prayekti, Endah; Lukiyono, Yauwan Tobing
RIGGS: Journal of Artificial Intelligence and Digital Business Vol. 4 No. 2 (2025): Mei - Juli
Publisher : Prodi Bisnis Digital Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/riggs.v4i2.524

Abstract

Analisis kelayakan usaha ini dilakukan sebagai upaya untuk menentukan potensi kelayakan suatu produk yang dikembangkan oleh industri, baik berskala besar maupun industri rumah tangga. Penelitian ini bertujuan untuk menghitung dan menilai kelayakan usaha dari produk minuman herbal NU NanoFit yang diproduksi oleh Program Studi D-IV Analis Kesehatan Universitas Nahdlatul Ulama Surabaya (UNUSA). Produk ini merupakan inovasi minuman herbal yang berfungsi sebagai imunomodulator serta membantu melancarkan sistem pencernaan. Proses analisis kelayakan dilakukan melalui dua tahap utama, yaitu uji laboratorium untuk memastikan kualitas produk dan uji kelayakan pasar melalui penyebaran kuesioner guna mengukur minat konsumen serta menentukan segmentasi pasar yang tepat. Hasil uji sitotoksisitas menunjukkan bahwa formulasi produk ini aman untuk dikonsumsi, dengan nilai LC₅₀ > 1000 ppm, yang mengindikasikan bahwa produk tidak bersifat toksik bagi tubuh. Berdasarkan hasil analisis terhadap 106 responden, diketahui bahwa minat masyarakat terhadap produk ini cukup tinggi, dengan 39,6% responden menyatakan tertarik hingga sangat tertarik. Selain itu, mayoritas responden (60,4%) memilih rentang harga jual antara Rp5.000 hingga Rp10.000. Temuan ini menunjukkan bahwa produk NanoFit memiliki prospek yang menjanjikan untuk dikembangkan secara komersial dalam pasar minuman kesehatan berbasis herbal.
Effect Of Candida Albicans Cell Concentration On Dna Isolation Results Using Modified Alkaline Lysis Method Endah Prayekti; Nada Mufarrohah
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 9 No 2 (2025): Medical Technology and Public Health Journal September 2025
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v9i2.7328

Abstract

Candida albicans is one of the leading causes of morbidity and mortality in various countries, with the prevalence of infection reaching 17,640 cases with an average incidence of 1.5 million people, causing more than one billion people, and the adverse effects of fungal infections can threaten >1.6 million lives. This study aimed to determine the effect of Candida albicans cell concentration on DNA isolation results. The method used was the modified Alkaline lysis using cell cultures with concentrations of 10⁸, 10⁹, and 10⁰ cells/mL. The results showed that the growth curve of the sample showed an increase in cell concentration directly proportional to the absorbance of the UV-Vis spectrophotometer results. Cell suspensions in SDB media were incubated for 2-3 days, then the absorbance was measured, resulting in a value of 5.307 nm after multiplying the dilution factor. The results of Candida albicans DNA isolation were visualized using 2% agarose gel electrophoresis, with no DNA bands found in all samples and negative results. In contrast, smear bands appeared in positive controls. Analysis using a Nanodrop spectrophotometer showed the purity of DNA isolates at concentrations of 10⁸ and 10⁹ < 1.8, while at 10¹⁰ it was 1.82. The average DNA concentration obtained was < 100 ng/µL. Based on the results of statistical tests, it can be concluded that the p-value <0.05 indicates the effect of cell concentration on the results of DNA isolation using the modified Alkaline lysis method. Keywords: Cell concentration, Candida albicans, Alkaline lysis, DNA isolation
Co-Authors Akbar Reza Muhammad Akhmad, Nur Faradila Althofur Hilman Rifki Andini, Ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Ardyarini Dyah Savitri Arsya Tazkiya Asma' Denaya Psari Yuwono Awaliyah, Arisma Putri Baihaqi, Muhammad Azhari Brina Thursina Dibiasi Devita Rahma Putri Devyana Dyah Wulandari dr Hartatiek Nila,Sp.OG Elsya Octaviani Cantika Putri Endah Budi Permana Putri Ersalina Nidianti Eva Rosa Dwi Febrianti Evi Sylvia Awwalia Fadillah Triasmoro Fakurazi, Sharida Gilang Nugraha Gilang Nugraha Hamiduumajid Ballihg Ballihgoo Hamiduumajid Ballihg Ballihgoo Hidayat, Muhammad Taufiq Husein Firdaus Ibrahim Dwi Waluyo Putra Imraatul Mukarramah Indah Nur Kamaliyah Indaryanti, Reski Novita Junaedy, M Dwinanda Kamelia, Dina Kardina, Rizki Nurmalya Khiliah Navis Khiliah Navis Khusnah, Hidayatul Kifli, Nurolaini Lili Susan Rahmawati Luqiana Fauziah M Nasir Maat, Suprapto Maki Zamzam Mardhotillah, Rachma Rizqina Marinda Dwi Puspitarini Melindah, Winda Mia Puspitasari Mirshall Abiedama Prayoga Mochamad Faishal Riza Muhammad Afwan Romdloni Muhammad Taufiq Hidayat Muhammad Taufiq Hidayat Mukarramah, Imraatul Mutiarawati, Diah Titik Nada Mufarrohah Nandasari, Anggy Dwipa Nathalya Dwi Kartika Sari Navis, Khiliah Ngiluhtara Aditiya Putri Nisa, Fauziyatun Nunki, Nastasya Nur Naili Fahira Nur Sophia Matin Pratama, Nizar Revandini Prayoga, Mirshall Abiedama Prisnidiawati, Ajeng Puspitasari, Renny Novi Putri Lestari, Ajeng Anindhita Putri, Ananda Ghufron Yuana Putri, Nasya Desinta Qonita Iklila Maghfuroh Qotrinnada, Nabilah Rahmawati, Lili Susan Ramadani, Vania Nur Regina Ayu Fristiyanti Rhomadhoni , Muslikha Nourma Riza, Mochammad Faishal Rizki Jalil, Ike Wasilatur Rizki Nurmalya Kardina Romdhoni, Muhammad Afwan Rosiana Dewi Regita Sa'bania Hari Raharjeng Sabrina, Shinta Milenia Sadza, Lailatus Salfa Salsabilah Zain Salimi, Syafrina Maulidyatus Salman Firmansyah Sari, Devi Fitriana Satya Nugraha Wirayudha Seggaf Achmad Syah Septina Isro’ Hermawati Setiarsih, Dini Setyana Yuli, Azizah Ari Siti Husnul Khotimah Sofaria Munir, Ratna Sonia Fitriatul Qolbi Suliati Suliati Sunaryo, Merry Suryandaru, Suryandaru Syafiuddin, Achmad Tauziah, Amalia Thomas Sumarsono Thomas Sumarsono Thomas Sumarsono Tullah, Auria Hidayah Uliyatul Laili, Uliyatul Umaha, Farida Wandara Sekar Ayu Pramesti Wideistianti, Annisa Pricilya Widya Novita Sari Wiwik Winarningsih Wulan, Wieke Sri Wulandari, Dwi Agustin Yauwan Tobing Lukiyono Yuliana Yuliana Yuni Nur Malita Sari Zahrotul Illiyin Zain, R.A Wafiqah Aulia Zalfa, Anisatuz Zazabilla, Indira