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All Journal Darussalam Nutrition Journal Celebes Biodiversitas : Jurnal Sains dan Pendidikan Biologi Jurnal SainHealth Bioma : Jurnal Biologi dan Pembelajaran Biologi Jurnal Analis Medika Biosains (JAMBS) Jurnal Biomedika Medical Technology and Public Health Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Medical and Health Science Journal Community Development Journal Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Abdimas Madani dan Lestari (JAMALI) Amalee: Indonesian Journal of Community Research & Engagement Community Development Journal: Jurnal Pengabdian Masyarakat BERNAS: Jurnal Pengabdian Kepada Masyarakat Medicra (Journal of Medical Laboratory Science/Technology) Indonesia Berdaya Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Environmental and Toxicology Management (ETM) Journal of Health Community Service SWARNA Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Journal of Artificial Intelligence and Digital Business Jurnal Penelitian dan Kajian Ilmiah Kesehatan Politeknik Medica Farma Husada Mataram National Conference for Ummah BIOSAPPHIRE: Jurnal Biologi dan Diversitas SWARNA: Jurnal Pengabdian Kepada Masyarakat al Kimiya : Jurnal Ilmu Kimia dan Terapan
Prayekti, Endah
Universitas Nahdlatul Ulama Surabaya
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Herbal Plant Education and Hydroponic Training of Vertical and Wick System in Limited Space Andini, Ary; Prayekti, Endah; Romdloni , Muhammad Afwan; Rhomadhoni , Muslikha Nourma; Sofaria Munir, Ratna
Community Development Journal Vol 8 No 1 (2024): Community Development Journal
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/cdj.v8i1.5619

Abstract

The program "Empowering the Community of Simo Angin-Angin Village, Sidoarjo through the Use of Natural Materials to Achieve Health Independence" was implemented at the Simo Angin-Angin Village Hall, Krian District, Sidoarjo Regency in May-June 2023. This Community Empowerment Program (Community Service) carries a preventive and consists of a series of activities, including counseling about the use of natural materials based on the recommendations of the Koran, considering that the majority of Simo Angin-Angin village residents are Muslim. This activity also includes training in farming using the hydroponic method. The aim of this program is to strengthen health independence in Simo Angin-Angin Village in order to improve collective welfare. The Community Service program in Simo Angin-Angin Village was attended by 22 participants, consisting of health cadres and the general public. The implementation is divided into 2 stages, namely counseling regarding the Black Cumin herbal plant and training regarding vertical hydroponic procedures, as well as the use of plastic bottles with the Wick system. The evaluation results showed that participants' understanding was very good, with 95% of them achieving a score of 100, while 5% obtained a score of 80. The evaluation results of participant satisfaction with the implementation of the extension showed very good scores for the topic material and delivery of the material by resource persons on herbal plant education and hydroponic training. This Community Service activity provides skills for the community to grow crops on narrow land using a hydroponic system
NEW PRIMER DESIGN of MULTIPLEX POLYMERASE CHAIN REACTION (PCR) for DETECTION of Escherichia Coli and Salmonella Enterica in FOOD SAMPLE Hidayat, Muhammad Taufiq; Prayekti, Endah; Lukiyono, Yauwan Tobing; Romdhoni, Muhammad Afwan; Sari, Devi Fitriana
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page17-25

Abstract

Background: Multiplex PCR techniques are used to detect multiple pathogens at the same time simultaneously. In order to achieve the specificity and sensitivity of detection, it is important to optimize the multiplex PCR method properly. Developing suitable primers and optimizing PCR temperature to boost particular genes from pathogens are key factors for this optimization. Methods: For the simultaneous detection of Escherichia coli and Salmonella enterica in food samples, this research aims to develop a set of primers for multiplex polymerase chain reaction (PCR). Using Primer-BLAST software, the study utilizes specific primer designs for the phoA gene (Eschericia coli) and invA gene (Salmonella enterica). DNA isolation has confirmed successful extraction from the two bacterial samples. PCR was performed under different conditions, including Singleplex and Multiplex PCR, using two annealing temperatures of 53oC and 50oC. Results: The results showed that this method can effectively amplify target genes and indicate their specificity and reliability at both temperature levels. Given these results, it has successfully conducted multiplex PCR using the built primer pairs. Both annealing temperatures of 50oC and 53oC can be used to perform multiplex PCR to detect E. coli and Salmonella enterica in one PCR reaction. Conclusion: Through this research, we have created a new set of Multiplex PCR Primers and an optimized multiplex PCR technique for the simultaneous detection of E coli and Salmonella enterica in food samples. The rapid and simultaneous screening of E. coli and S. enterica, which contributes to improved food safety measures and pathogen detection in the food industry, is promising with this optimized PCR approach.
Effectiveness of the repellent of kawista leaf extract (Limonia acidissima) against Aedes aegypti mosquitoes Qotrinnada, Nabilah; Lukiyono, Yauwan Tobing; Prayekti, Endah; Nidianti, Ersalina
Environmental and Toxicology Management Vol. 3 No. 2 (2023): Environmental drivers of vector-borne: Impact and mitigation
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/etm.v3i2.6390

Abstract

Synthetic insecticides containing highly effective active substances such as DEET (N,N-diethyl-meta toluamide) to prevent and control Dengue Hemorrhagic Fever can have adverse effects on health and the environment. Kawista leaves (Limonia acidissima) have the potential as a natural insecticide against Aedes aegypti mosquitoes because they contain secondary metabolite compounds namely phenols, flavonoids, saponins, and terpenoids. This study aims to determine the effectiveness of the repellent power of kawista leaf extract (Limonia acidissima) against Aedes aegypti mosquitoes. This study used experimental method with post-test only control group design. The sample used was 25 adult of Aedes aegypti mosquitoes in each treatment. The ethanol extract of kawista leaves was made in the form of a spray with a concentration of 25%, 50% concentration, and 75% concentration. The 25% concentration, 50% concentration, and 75% concentration of kawista leaf extract have an effective repellent power against Aedes aegypti mosquitoes, namely 15.22%, 46.20%, and 70.08%, respectively. The results of the analysis of kawista leaf extract against Aedes aegypti mosquitoes obtained ED50 (Effective Dose 50) at a concentration of 60.343% and ED99 (Effective Dose 99) at a concentration of 124.452%. From the results of the study, it can be concluded that kawista leaves have the ability to repel Aedes aegypti mosquitoes.
Relationship between personal hygiene and manifestation of mite (Sarcoptes scabiei) in students at islamic boarding school in Sidoarjo City Wideistianti, Annisa Pricilya; Lukiyono, Yauwan Tobing; Andini, Ary; Prayekti, Endah
Environmental and Toxicology Management Vol. 3 No. 2 (2023): Environmental drivers of vector-borne: Impact and mitigation
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/etm.v3i2.6391

Abstract

Scabies is a disease that attacks the skin in humans, a disease caused by the mite, included in the ovoid Arthropod organism. Personal hygiene is a way to maintain health and cleanliness of the body with self-care, therefore the better a person maintains personal hygiene, the less exposure to mites that enter the skin, if someone does not maintain personal hygiene, it will be very easy to get scabies. This study aims to determine the relationship between personal hygiene and the manifestation of mites using the burrow ink test method on students at the Islamic Boarding School in Sidoarjo City. The method used is an observational type that is analytical using a cross-sectional design. A sample of 45 samples in March 2024. The sampling method was carried out using a non-probability sampling method. The samples were examined using the burrow ink test method and continued with skin scraping examination. The results of the examination of 45 samples showed that there were 9 respondents in the good category of personal hygiene clothing cleanliness with a positive scabies examination of 27 (60%) and there were 25 respondents in the poor category of personal hygiene clothing cleanliness with a negative scabies examination of 41 (91%). The results of statistical analysis from the chi-square test, the personal hygiene variable with the manifestation of mites produced a p-value = 0.157 which means there is no significant effect. In this study it can be concluded that there is no relationship between personal hygiene using the burrow ink test method and the incidence of scabies in Islamic Boarding Schools in Sidoarjo City.
Comparison of the insidence of toxoplasmosis in feature samples of house cats and market cats in Sidoarjo City Zazabilla, Indira; Lukiyono, Yauwan Tobing; Santoso, Andreas Putro Ragil; Prayekti, Endah
Environmental and Toxicology Management Vol. 3 No. 3 (2023): Soil Health, Animal Health, and Water Quality in Agroecological Systems
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/etm.v3i3.6459

Abstract

To determine the comparison of toxoplasmosis in the feces of cats house and cats market in the Sidoarjo City. This method is a direct slide method using a direct preparation using a 2% Eosin solution. The principle of stool examination uses a direct method that uses a microscope to determine stool that is positive for Toxoplasmosis. The results of this research were obtained in the form of primary data, namely the results of a microscope examination. There were 16 fecal samples taken by purposive sampling in the Sidoarjo City. This research was conducted at the Parasitology Laboratory at Universitas Nahdlatul Ulama Surabaya, Surabaya. Cats are animals that are very popular as pets. Cats can transmit zoonotic diseases, namely toxoplasma, which is caused by the Toxoplasmosis parasite. Toxoplasmosis infection is an intracellular parasite from the group of protozoa which is an obligate parasite, where cats act as definitive hosts. Results on the incidence of Toxoplasmosis using feces samples from cats house and cats market found no Toxoplasmosis infection.
PERBEDAAN HASIL KADAR HEMOGLOBIN MENGGUNAKAN HEMATOLOGY ANALYZER DAN POCT Zalfa, Anisatuz; Nugraha, Gilang; Wulan, Wieke Sri; Prayekti, Endah
Jurnal Penelitian dan Kajian Ilmiah Kesehatan Politeknik Medica Farma Husada Mataram Vol. 10 No. 2 (2024): Oktober
Publisher : Politeknik Medica Farma Husada Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33651/jpkik.v10i2.548

Abstract

Hemoglobin is a protein molecule in red blood cells that carries O2 throughout the body. Hemoglobin contains iron which makes blood red. The hemoglobin level is a measure of the respiratory pigment present in the erythrocytes, which is used to determine anemia. This study was to see if there were differences in the results of hemoglobin levels using a hematology analyzer and POCT. This study used a hematology analyzer and POCT to determine the accuracy of the results obtained on the examination of hemoglobin levels. The aim of this study was to determine differences in the results of hemoglobin levels using a hematology analyzer and POCT. This type of research is analytic observational with a cross sectional approach. This study used venous blood samples using the hematology analyzer method and capillary blood samples using the POCT method as many as 54 samples. Collecting data using purposive sampling technique. The research was conducted in May 2023. The analytical test used the Independent T Test. Based on the research results, the p-value = 0.212 so that H0 is accepted, which means that there is no difference in the results of hemoglobin levels using a hematology analyzer and POCT. Based on the independent t test, the results obtained were a p-value of 0.212, which means that there was no difference in the results of hemoglobin levels using a hematology analyzer and POCT.
ANALISIS KANDUNGAN LEMAK TIDAK JENUH PADA KUE SEMPRONG BERBASIS CAMPURAN TEPUNG BIJI BUNGA MATAHARI DAN QUINOA Raharjeng, Sa'bania Hari; Kardina, Rizki Nurmalya; Prayekti, Endah; Tullah, Auria Hidayah; Sabrina, Shinta Milenia
Darussalam Nutrition Journal Vol. 8 No. 2 (2024): Darussalam Nutrition Journal
Publisher : Universitas Darussalam Gontor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/dnj.v8i2.11409

Abstract

Latar belakang: Angka kejadian penyakit tidak menular semakin meningkat dan berpotensi mengarah pada permasalahan kesehatan serta komplikasi penyakit lainnya. Kebiasaan konsumsi camilan dari bahan makanan tinggi lemak jenuh menjadi salah satu penyebab terjadinya permasalahan tersebut. Kebutuhan akan camilan yang lebih sehat dengan kandungan lemak tidak jenuh perlu menjadi perhatian. Tujuan: Penelitian ini bertujuan untuk menganalisis perbedaan kandungan zat gizi Polyunsaturated Fatty Acids (PUFA) dan Monounsaturated Fatty Acids (MUFA) pada kue semprong berbasis tepung biji bunga matahari dan tepung quinoa. Metode: Penelitian ini merupakan penelitian eksperimental dengan perlakuan berupa perbedaan komposisi tepung biji bunga matahari dan tepung quinoa dengan rasio Formula 1 (100:0), Formula 2 (75:25), dan Formula 3 (0:100). Hasil uji kandungan zat gizi dianalisis menggunakan uji one-way ANOVA. Hasil: Penggunaan tepung biji bunga matahari dan tepung quinoa sebagai pengganti tepung beras pada kue semprong menunjukkan hasil perbedaan kandungan Polyunsaturated Fatty Acids (PUFA) dan Monounsaturated Fatty Acids (MUFA) yang signifikan antar kelompok perlakuan (p<0,05). Formula 1 rata-rata mengandung 20,48g PUFA dan 9,62g MUFA; Formula 2 rata-rata mengandung 14,86g PUFA dan 8,27g MUFA; Formula 3 rata-rata mengandung 3,17g PUFA dan 4,27g MUFA.  Simpulan: Formula terbaik ada pada Formula 1 yang mengandung PUFA dan MUFA paling tinggi. Sedangkan formula 3 mengandung PUFA dan MUFA paling rendah. Semakin tinggi kandungan tepung biji bunga matahari dalam formula, maka kandungan PUFA dan MUFA akan semakin tinggi. Kata Kunci : biji bunga matahari, kue semprong, monounsaturated fatty acids, polyunsaturated fatty acids, quinoa
Detection of mecA Genes from Staphylococcus aureus Bacteria in Raw Cow Milk in the Surabaya Regional Farm Putri Lestari, Ajeng Anindhita; Prayekti, Endah Prayekti; Hidayat, Muhammad Taufiq
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.416

Abstract

Milk is a very important food ingredient in meeting nutritional needs and a good growth medium for microorganisms. Staphylococcus aureus bacteria are pathogens that often contaminate milk. Staphylococcus aureus bacteria can cause a disease called mastitis. The acropolis is very susceptible to infections, treatment and prevention need to be done to avoid mastitis. Treatment of mastitis until now still uses antibiotics, improper use of antibiotics can cause Staphylococcus aureus resistance to antibiotics. The mecA gene is the gene responsible for beta-lactam antibiotic resistance. This study aims to determine the presence of the mecA gene in Staphylococcus aureus isolate from raw cow's milk. The samples from this study are 24 samples taken from dairy farms in the Surabaya Region. Isolation of Staphylococcus aureus bacteria by conventional microbiology based on culture properties, Gram staining and biochemical tests. The results of this study showed that there were 5 samples that were positive for Staphylococcus aureus. Molecular detection by PCR of the mecA gene in Staphylococcus aureus was not found in the five Staphylococcus aureus isolates. Based on this study has the result that the sample found Staphylococcus aureus but no mecA gene was found.
Pemberdayaan Masyarakat: Upaya Peningkatan Kompetensi Praktisi Kesehatan dalam Analisis Molekuler untuk Diagnosis dan Penatalaksanaan Penyakit: Indonesia Andini, Ary; Nugraha, Gilang; Hidayat, Muhammad Taufiq; Prayekti, Endah; Salimi, Syafrina Maulidyatus; Navis, Khiliah; Sari, Widya Novita; Fakurazi, Sharida
Jurnal Abdimas Madani dan Lestari (JAMALI) Volume 07, Issue 01, Maret 2025
Publisher : UII

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jamali.vol7.iss1.art5

Abstract

Molecular analysis has played a pivotal role in diagnosing and managing diseases during pandemics by enabling early outbreak detection and rapid identification of disease causes, leading to prompt treatment. However, there is still a pressing need to enhance the understanding and proficiency of molecular diagnostics in Southeast Asia. Therefore, proactive measures should be taken to improve comprehension through seminars and training programs focused on molecular analysis. The objective is to strengthen the diagnostic and management capabilities of healthcare workers concerning infectious diseases in Southeast Asia, ultimately aiming to enhance the overall health of the population. The target participants include healthcare professionals, medical laboratory technicians, educators, and students. Through collaboration with Universiti Putra Malaysia, two significant events were organized. The International Seminar on Medical Laboratory Technology (ISoMLatech), took place on July 5, 2023, with the theme "Advancements in Molecular Assay for Diagnosing and Managing Infectious Diseases in Southeast Asia." This event was held in a hybrid format, utilizing Zoom and the UNUSA Auditorium, and it attracted 510. Afterwards, a Short Course was conducted since August 13 to 15, 2023, at Universiti Putra Malaysia (UPM) with 22 participants, The materials received were as followed: the fundamentals of molecular examinations, immunochemical detection, epidemiology of infectious diseases, antimicrobial resistance, and visits to UPM Campus and Institute of Bioscience, UPM. The evaluation results indicate that over 75% of participants rated both ISoMLatech and Short Course as good or very good. Consequently, there is strong anticipation for the continued success and implementation of the program.
Stimulation of Creative Entrepreneurship: Utilizing Natural Materials in Healthcare through a Nanotechnology Approach Andini, Ary; Prayekti, Endah; Wulandari, Devyana Dyah; Romdloni , Muhammad Afwan; Syafiuddin, Achmad; Sari, Widya Novita; Zain, R.A Wafiqah Aulia; Akhmad, Nur Faradila; Suryandaru, Suryandaru; Kifli, Nurolaini
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 1 (2025)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

As the world becomes increasingly modern, technology continues to advance at a rapid pace. The rise of Artificial Intelligence (AI) has contributed to a decline in job opportunities, leading to an increase in unemployment rates. In response, the D-VI Health Analyst Study Program at UNUSA aims to nurture young entrepreneurs in the health sector by organizing seminars and training sessions on creative entrepreneurship. These focus on the utilization of natural materials in health services through a nanotechnology approach, targeting young people, medical laboratory technicians, lecturers, and students. The event featured speakers from industry and international experts to expand the knowledge of youth and the broader community. It was conducted online as part of the International Seminar on Medical Laboratory Technology (ISoMLatech), held on March 6, 2024, via Zoom, with 216 internal participants. Evaluation results showed that over 75% of participants rated the ISoMLatech event as good or very good. It is hoped that such activities will continue in the future, as they play a crucial role in enhancing the knowledge and skills of healthcare workers in advancing nanotechnology innovation within the global healthcare industry. Keyword: Nanotechnology, Natural product, Entrepreuner, Healtcare
Co-Authors Akbar Reza Muhammad Akhmad, Nur Faradila Andini, Ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Ardyarini Dyah Savitri Arsya Tazkiya Awaliyah, Arisma Putri Baihaqi, Muhammad Azhari Brina Thursina Dibiasi Devita Rahma Putri Devyana Dyah Wulandari dr Hartatiek Nila,Sp.OG Dwi Agustin Wulandari Elsya Octaviani Cantika Putri Endah Budi Permana Putri Ersalina Nidianti Eva Rosa Dwi Febrianti Evi Sylvia Awwalia Fadillah Triasmoro Fakurazi, Sharida Fauziah, Luqiana Fristiyanti, Regina Ayu Gilang Nugraha Hamiduumajid Ballihg Ballihgoo Hamiduumajid Ballihg Ballihgoo Hermawati, Septina Isro’ Hidayat, Muhammad Taufiq Husein Firdaus Ibrahim Dwi Waluyo Putra Indah Nur Kamaliyah Indaryanti, Reski Novita Junaedy, M Dwinanda Kamelia, Dina Kardina, Rizki Nurmalya Khusnah, Hidayatul Kifli, Nurolaini M Nasir Maat, Suprapto Maghfuroh, Qonita Iklila Mardhotillah, Rachma Rizqina Marinda Dwi Puspitarini Matin, Nur Sophia Melindah, Winda Mirshall Abiedama Prayoga Mochamad Faishal Riza Muhammad Afwan Romdloni Muhammad Taufiq Hidayat Mukarramah, Imraatul Mutiarawati, Diah Titik Nada Mufarrohah Nandasari, Anggy Dwipa Nastasya Nunki Nathalya Dwi Kartika Sari Navis, Khiliah Ngiluhtara Aditiya Putri Nisa, Fauziyatun Nur Naili Fahira Pratama, Nizar Revandini Prayoga, Mirshall Abiedama Prisnidiawati, Ajeng Puspitasari, Mia Putri Lestari, Ajeng Anindhita Putri, Ananda Ghufron Yuana Putri, Nasya Desinta Qotrinnada, Nabilah Rahmawati, Lili Susan Ramadani, Vania Nur Rhomadhoni , Muslikha Nourma Riza, Mochamad Faishal Riza, Mochammad Faishal Rizki Jalil, Ike Wasilatur Rizki Nurmalya Kardina Romdhoni, Muhammad Afwan Sa'bania Hari Raharjeng Sabrina, Shinta Milenia Sadza, Lailatus Salfa Salsabilah Zain Salimi, Syafrina Maulidyatus Salman Firmansyah Sari, Devi Fitriana Satya Nugraha Wirayudha Setiarsih, Dini Setyana Yuli, Azizah Ari Siti Husnul Khotimah Sofaria Munir, Ratna Sonia Fitriatul Qolbi Suliati Suliati Sunaryo, Merry Suryandaru, Suryandaru Syafiuddin, Achmad Syah, Seggaf Achmad Tauziah, Amalia Thomas Sumarsono Thomas Sumarsono Thomas Sumarsono Tullah, Auria Hidayah Uliyatul Laili, Uliyatul Umaha, Farida Wandara Sekar Ayu Pramesti Wideistianti, Annisa Pricilya Widya Novita Sari Wiwik Winarningsih Wulan, Wieke Sri Yauwan Tobing Lukiyono Yuliana Yuliana Yuni Nur Malita Sari Yuwono, Asma' Denaya Psari Zahrotul Illiyin Zain, R.A Wafiqah Aulia Zalfa, Anisatuz Zazabilla, Indira