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<![CDATA[UJI SENSITIVITAS PROPOLIS TERHADAP BAKTERI Staphylococcus aureus Dan Eschericia coli ]]>
<![CDATA[Anindya, Lokawanti Al Fahna]]>;
<![CDATA[Pestariati, Pestariati]]>;
<![CDATA[Wisnu, Istanto]]>
<![CDATA[ Analis Kesehatan Sains Vol. 10 No. 2 (2021): Analis Kesehatan Sains ]]>
Publisher : <![CDATA[Politeknik Kesehatan Kementerian Kesehatan Surabaya ]]>
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DOI: 10.36568/anakes.v10i2.18
<![CDATA[The threat of infectious diseases from strains of pathogenic bacteria Staphylococcus aureus and Eschericia coli is capable of causing resistance to antibiotics. The prevalence of MRSA cases was 8.2%. One of the efforts to control infections caused by antibiotic-resistant bacteria is to utilize several antimicrobial compounds derived from natural ingredients, namely propolis. Contains polyphenolic compounds and flavonoids that function as antimicrobials. The purpose of this study was to analyze the inhibition zone formed due to the antimicrobial activity of propolis extract against the growth of Staphylococcus aureus and Eschericia coli bacteria. This research was conducted in vitro at the Microbiology Laboratory of the Medical Laboratory Technology Department of Health Poltekkes Surabaya in February – April 2021. This study used the Kirby Bauer diffusion method with paper blank discs on MHA media by measuring the diameter of the inhibition zone formed from the growth of Staphylococcus aureus and Eschericia coli with units of mm. Research The concentration of propolis extract used was 5%, 10%, 15%, and 20%. In this study, it was concluded that propolis extract can be used as an antimicrobial compound, there is a different effect of concentration of propolis extract on Staphylococcus aureus from Eschericia coli. Data analysis on the Kruskal Wallis test has a value of 0.004 (<0.05) which indicates that there is a significant difference between each concentration of propolis extract both against Staphylococcus aureus and Eschericia coli. The effect of the effective concentration is at a concentration of 20% against Staphylococcus aureus which has an average inhibition zone diameter of 13 mm while that of Eschericia coli is 22.5 mm.]]>
<![CDATA[Pemanfaatan Kacang Kedelai (Glycine max (L.) Merill) Varietas Anjasmoro dan Dega-1 Sebagai Media Alternatif PDA (Potato Dextrose Agar) untuk Pertumbuhan Candida Albicans ]]>
<![CDATA[Putri, Ervina Wahyu]]>;
<![CDATA[Sasongkowati, Retno]]>;
<![CDATA[Astuti, Sri Sulami Endah]]>;
<![CDATA[Istanto, Wisnu]]>
<![CDATA[ Jurnal Penelitian Kesehatan SUARA FORIKES 2023 ]]>
Publisher : <![CDATA[FORIKES ]]>
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DOI: 10.33846/sf14nk123
<![CDATA[PDA (Potato Dextrose Agar) is a medium containing many nutrients needed by fungi, especially Candida albicans. Several researchers found alternative media for fungal growth using various carbohydrate sources such as cassava dextrose agar, canna tubers, yam tubers and arrowroot tubers on Candida albicans and Aspergillus niger. One of the components contained in PDA media is carbohydrates. Soybeans (Gllycine Max L. Merill) are a source of carbohydrates which are usually used as ingredients for food and beverage preparations (such as soy sauce, tempeh, tofu, and milk). Several soybean varieties that are included in the top 10 best quality with high nutritional content are the Anjasmoro variety and the Dega-1 variety. This study aimed to answer that the soybean (Glycine max (L.) Merill) varieties Anjasmoro and Dega-1 can be used as an alternative PDA medium for the growth of Candida albicans. The research method used was experimental to test the potential of the Anjasmoro variety and the Dega-1 variety as alternative media for PDA by analyzing the growth characteristics of Candida albicans with variations in the mass of soybean powder for each variety. The results showed that soybean variety Anjasmoro variety was better than soybean variety Dega-1 and has the potential to be an alternative media because the average number of colonies and morphology of Candida albicans at 4.4 gram mass variation was almost equivalent to the fungus growing on Gold media standard.Keywords: Candida albicans; Potato Dextrose Agar; soybean variety Anjasmoro; soybeans of the Dega-1 variety ABSTRAK PDA (Potato Dextrose Agar) merupakan media berisi banyak kandungan nutrisi yang dibutuhkan oleh jamur terutama Candida albicans. Beberapa peneliti menemukan media alternatif untuk pertumbuhan jamur menggunakan berbagai sumber karbohidrat seperti singkong dextrose agar, umbi ganyong, umbi gembili dan umbi garut pada Candida albicans dan Aspergillus niger. Salah satu komponen yang terdapat dalam media PDA yaitu karbohidrat. Kacang kedelai (Gllycine Max L. Merill) merupakan sumber karbohidrat yang biasanya dimanfaatkan sebagai bahan olahan makanan dan minuman (seperti kecap, tempe, tahu, dan susu). Beberapa varietas kacang kedelai yang termasuk 10 besar kualitas terbaik dengan kandungan gizi yang tinggi yaitu varietas Anjasmoro dan varietas Dega-1. Penelitian ini bertujuan untuk menjawab bahwa kacang kedelai (Glycine max (L.) Merill) varietas Anjasmoro dan Dega-1 dapat dimanfaatkan sebagai media alternatif PDA untuk pertumbuhan Candida albicans. Metode penelitian yang digunakan adalah eksperimental untuk menguji potensi kacang kedelai varietas Anjasmoro dan Varietas Dega-1 sebagai media alternatif PDA dengan menganalisis karakteristik pertumbuhan Candida albicans dengan variasi massa serbuk kacang kedelai masing-masing varietas. Hasil penelitian menunjukkan bahwa kacang kedelai varietas Anjasmoro lebih baik dibandingkan kacang kedelai varietas Dega-1 dan berpotensi untuk menjadi media alternatif dikarenakan pada rata-rata jumlah koloni dan morfologi Candida albicans pada variasi massa 4,4 gram hampir setara dengan jamur yang tumbuh pada media Gold standart.Kata kunci: Candida albicans; Potato Dextrose Agar; kacang kedelai varietas Anjasmoro; kacang kedelai varietas Dega-1]]>
<![CDATA[Peningkatan Kemandirian Siswa dalam Menerapkan Pola Hidup Bersih dan Sehat untuk Mencegah dan Mengurangi Infeksi Soil Transmitted Helminths (Sth) di SD MI Sunan Ampel 1 Sidorogo-Trosobo Kecamatan Taman Kabupaten Sidoarjo ]]>
<![CDATA[Suliati]]>;
<![CDATA[Wisnu Istanto]]>;
<![CDATA[Anita Dwi Anggraini]]>
<![CDATA[ ABDIKESMAS MULAWARMAN : Jurnal Pengabdian Kepada Masyarakat Vol.3 No.1 Mei (2023) : ABDIKESMAS MULAWARMAN ]]>
Publisher : <![CDATA[Universitas Mulawarman ]]>
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DOI: 10.30872/abdikesmasmulawarman.v3i1.156
<![CDATA[Soil Transmitted Helminths (STH) adalah golongan cacing usus (Nematoda Usus) dalam perkembanganya membutuhkan tanah untuk men jadi bentuk infektif. Golongan STH yang habitatnya pada usus manusia adalah Ascaris lumbricoides, Hookworm (Necator americanus dan Ancylostoma duodenale), Strongiloides stercoralis, Trichuris trichiura. Madrasah Ibtidaiyah Sunan Ampel II mulai kiprahnya dalam dunia pendidikan sejak tahun1988 atas prakarsa beberapa tokoh agama, tokoh masyar akat dan adanya tuntutan dari masyarakat setempat. Mengingat semakin banyaknya output yang dihasilkan. Madrasah Ibtidaiyah Sunan Ampel II setiap tahunnya, juga kondisi masyarakat setempat yang tergolong mene ngah kebawah, sedangkan letak desa tersebut jauh dari lembaga pendidikan SMP dan setingkatnya yang ada disekitar desa tersebut. Penelitian dilakukan oleh Megadkk tahun 2020 di SD MI Sunan Ampel 1 Desa Sidorogo Kecamatan Taman Kabupaten di didapatkan hasil terdapat siswa sebanyak 44% terinfeksi STH, hal ini dikarenakan siswa yang kurang pengetahuan tentang kecacingan sebanyak 19 siswa (76%), sedangkan cukupnya pengetahuan tentang kecacingan sebanyak 4 siswa (16%), dan baiknya pengetahuan tentang kecacingan sebanyak 2 siswa (8%). dengan jumlah keseluruhan sebanyak 25 anak (100%). sebagian besar siswa maupun orang tua belum mengenal jenis-jenis cacing yang berbahaya bagi kesehatan, ataupun mengetahui cara pencegahan dan akibat kecacingan. Upaya yang dapat dilakukan adalah kegiatan penyuluhan yang berlangsu ng sangat interaktif dan komunikatif dan setiap peserta penyuluhan dapat memahami penjelasan yang disampaikan baik melalui media poster baik melalui tanya jawab. Berdasarkan hasil pretest dan post terdapat peningkatan pengetahun rata-rata 23% menjadi 71% ,sehingga total peningkatan pengetahuan peserta bertambah sebesar 48%.]]>
<![CDATA[The Approval of Reading Speed Capacities for Laboring Textbooks to Improve Reading Speed for Laboring Textbooks for Students of Vocational Health ]]>
<![CDATA[Era Yunita Fitria]]>;
<![CDATA[Wisnu Istanto]]>;
<![CDATA[Noer Amelia]]>;
<![CDATA[Merlissa Suemith]]>
<![CDATA[ Bulletin of Science Education Vol. 4 No. 2 (2024): Bulletin of Science Education ]]>
Publisher : <![CDATA[CV. Creative Tugu Pena ]]>
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DOI: 10.51278/bse.v4i2.1431
<![CDATA[If students are to pursue vocational health or any other technical area of specialization, then they have to acquire effective reading skills. Most learners experience difficulties in speed and comprehension, which impacts negatively on both academic and professional pursuits later in life. This problem is exacerbated by the technical nature of the language used in vocational health education and the bulk of information carried within textbooks, which is imperative in translating classroom knowledge into practice. This study aims to improve the reading speed of labor-intensive textbooks that vocational health students go through, as a professional would face situations in the field where interpreting data speedily is inevitable. While reading speed and comprehension remain critical, remarkably few published studies have covered this topic in vocational health. The present study fills that gap by examining some interventions for improving reading speed for the type of learner under study. This present study employed a mixed-methods approach, which involved gathering both qualitative and quantitative data. For the current study, the participants included 78 vocational health students. An experimental group received targeted speed-reading and comprehension strategies, while the control group followed their normal study routine. Descriptive statistics, T-tests, and correlation analyses were applied to analyze pre-test and post-test data on reading speed and comprehension, together with students' feedback. While the control group indicated very slight improvements, the experimental group showed significant gains in reading speed and comprehension, thus targeted interventions could work well.]]>
<![CDATA[THE EFFECT OF DELAY IN TEMPERATURE VARIATIONS ON RESULTS OF GLUCOSE TEST IN DM PATIENTS WITH HYPERCHOLESTEROLEMIA ]]>
<![CDATA[Khotul Ula, Chori]]>;
<![CDATA[Edy Haryanto, Edy Haryanto]]>;
<![CDATA[Syamsul Arifin, Syamsul Arifin]]>;
<![CDATA[Istanto, Wisnu]]>
<![CDATA[ Journal of Vocational Health Studies Vol. 7 No. 1 (2023): July 2023 | JOURNAL OF VOCATIONAL HEALTH STUDIES ]]>
Publisher : <![CDATA[Faculty of Vocational Studies, Universitas Airlangga ]]>
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DOI: 10.20473/jvhs.V7.I1.2023.10-16
<![CDATA[Background: The pre-analytic stage has the most significant contribution to error, which is 60% to 70%. This is an excellent contribution to the dependability of laboratory results. Delays in the examination often occur in the laboratory due to various factors influencing glucose examination outcomes. Purpose: To ascertain the effect of long delays in various temperatures on the results of plasma NaF and serum glucose tests in diabetes mellitus patients who have hypercholesterolemia. Method: Pre-test and post-test design used in a pre-experimental method. In the pretest, plasma NaF and serum glucose levels were checked immediately. In the posttest, the plasma NaF and serum samples were delayed for 4 and 8 hours at the temperature of the refrigerator (4°C) and room temperature (25°C). Result: The results of One-way ANOVA test analysis of the plasma NaF glucose level with an immediate examination, a delay of 4 and 8 hours at room temperature (25°C) showed no effect with a Sig. of 0.423 > 0.05. Plasma NaF glucose level with an immediate examination, 4 and 8 hours delay in refrigerator temperature (4°C) showed no effect with Sig. of 0.772 > 0.05. Serum glucose levels with an immediate examination, 4 and 8 hours delay at room temperature (25°C) showed no effect with a Sig. of 0.333 > 0.05. Serum glucose levels with an immediate examination, 4 and 8 hours delay in refrigerator temperature (4°C) showed no effect with a Sig. of 0.604 > 0.05. Conclusion: There was no effect of 4 hours and 8 hours of delay at the temperature of the refrigerator (4°C) and room temperature (25°C) on the results of NaF and serum glucose examinations in patients with diabetes mellitus who have hypercholesterolemia.]]>
<![CDATA[DETECTION OF PANTON-VALENTINE LEUKOCIDIN (PVL) GENE AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN DIABETIC ULCER PATIENTS ]]>
<![CDATA[Prisma Anjarlena]]>;
<![CDATA[Suliati]]>;
<![CDATA[Wisnu Istanto]]>;
<![CDATA[Retno Sasongkowati]]>
<![CDATA[ Journal of Vocational Health Studies Vol. 7 No. 1 (2023): July 2023 | JOURNAL OF VOCATIONAL HEALTH STUDIES ]]>
Publisher : <![CDATA[Faculty of Vocational Studies, Universitas Airlangga ]]>
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DOI: 10.20473/jvhs.V7.I1.2023.17-23
<![CDATA[Background: Diabetes Mellitus (DM) patients are susceptible to wound infections brought on by S. aureus bacteria, in dealing with infections caused by bacteria, antibiotic treatment was conducted. Nevertheless, misapplication of antibiotics might lead to drug resistance or MRSA. Panton-Valentine Leukocidin (PVL) is a crucial factor of S. aureus pathogenicity. Skin and soft tissue infections can be caused by the PVL gene. Purpose: To detect PVL gene in ulcer swabs of diabetic mellitus patients against MRSA strains. Method: Employing descriptive quantitative data analysis of PVL gene detection observations on ulcer swab of diabetes mellitus patients using the RT-PCR method. Ulcer swab samples were collected at Dharmahusada and Banyu Urip Diabetes Wound Specialist Hospital, Surabaya, East Java, Indonesia. The research was conducted at the Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, Bacteriology Laboratory for the isolation and identification of MRSA and Molecular Biology Laboratory for the detection of the PVL gene in April–May 2022. Result: From 30 samples of ulcer swabs, 8 samples were positive for MRSA and 22 samples were negative for MRSA. After that, the MRSA positive samples were detected using the RT-PCR method to detect the PVL gene. The results of PVL detection were obtained from 6 samples (75%) positive for detecting the PVL gene and 2 samples (25%) negative for detecting the PVL gene. Conclusion: The results of the study showed that ulcer swabs from diabetes mellitus patients had the PVL gene against MRSA.]]>
<![CDATA[Deteksi Gen blaTEM Dari Bakteri Klebsiella Pneumoniae Penghasil Extended Spectrum Beta-Lactamase (ESBL) Pada Pasien Infeksi Saluran Kemih ]]>
<![CDATA[Widyawati, Keri Ayu]]>;
<![CDATA[Suliati]]>;
<![CDATA[Anggraini, Anita Dwi]]>;
<![CDATA[Wisnu Istanto]]>
<![CDATA[ Jurnal Media Analis Kesehatan Vol 15 No 2 (2024): JURNAL MEDIA ANALIS KESEHATAN ]]>
Publisher : <![CDATA[Potekkes Kemenkes Makassar ]]>
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DOI: 10.32382/jmak.v15i2.814
<![CDATA[Infeksi saluran kemih merupakan infeksi yang terjadi pada saluran uretra dan kandung kemih yang disebabkan oleh bakteri Klebsiella pneumoniae salah satunya. Pengobatan atau terapi yang sering digunakan untuk penderita infeksi saluran kemih yakni dengan pemberian antibiotik golongan beta-laktam. Namun, terdapat beberapa bakteri yang memiliki enzim beta-laktamase yang merupakan salah satu penyebab bakteri resisten terhadap antibiotik. Enzim tersebut dikodekan oleh gen salah satunya gen TEM yang dapat menghidrolisis antibiotik golongan beta-laktam. Tujuan studi ini untuk mendeteksi gen blaTEM terhadap bakteri Klebsiella pneumoniae penghasil Extended Spectrum Beta-Lactamase (ESBL) yang diisolasi dari urin pasien infeksi saluran kemih. Metode penelitian memanfaatkan Deskriptif kuantitatif dengan jumlah sampel penelitian 30 pasien infeksi saluran kemih di PSPAL Dr, Ramelan Surabaya. Studi ini dilakukan pada bulan Februari – Mei 2024. Identifikasi dan uji sensitivitas antibiotik memanfaatkan alat Vitek 2 Compact, uji konfirmasi ESBL menggunakan metode DDST, dan deteksi gen blaTEM menggunakan alat PCR Konvensional. Hasil penelitian menunjukkan bahwa terdapat 7(23%) sampel yang positif gen blaTEM, ditandai dengan terbentuknya pita DNA dengan panjang basa 445bp pada elektroforesis. Dapat disimpulkan bahwa gen blaTem pada bakteri Klebsiella pneumoniae terdeteksi sebagai produsen ESBL pada pasien infeksi saluran kemih]]>
<![CDATA[Promoting Storytelling in Enhancing Health Vocational Students’ Speaking Achievement ]]>
<![CDATA[Istanto, Wisnu]]>;
<![CDATA[Candra, Diah Arumita]]>;
<![CDATA[Heryani, Heni]]>
<![CDATA[ Anglophile Journal Vol. 2 No. 1 (2021): Anglophile Journal ]]>
Publisher : <![CDATA[CV. Creative Tugu Pena ]]>
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DOI: 10.51278/anglophile.v2i1.266
<![CDATA[Soft skills development requires the use of education media and forums. While storytelling may be used in competitions, media classes do not use it as a teaching media. Storytelling in the classroom hasn't been used to its fullest potential. English language proficiency can be improved through the use of digital storytelling, specifically on YouTube, by students. The study is intended to achieve similarities and discrepancies in the importance of the English-language achievement with general English and the implementation of digital storytelling. This study may be graded as an experimental investigation and classroom action research. This study employed cross-sectional research with independent speaking pre-test and post-test for experimental and control group design. Talking with storytelling therapy triggering different strategies is advised in experimental classes. It uses intervention research to change people's "talking," "doing" and "related practices". For the control group data combined with T-tests findings, a value of 0.417 has been achieved with an average value of -10.97436. In achieving speaking test outcomes, one may interpret that the treatment group has a more important meaning than the control group. The outcome of this study show that storytelling learning can help students at vocational health Level 2, more than the general English learning, achieve the high speaking achievement. Keywords: Promoting Storytelling, Speaking Achievement, Speaking Skill]]>
<![CDATA[Validation and Reliability of Self-Efficacy English Learning of Health Vocational Students with Jigsaw Learning Strategy ]]>
<![CDATA[Istanto, Wisnu]]>;
<![CDATA[Amelia, Noer]]>;
<![CDATA[Anggoro, Sapto Dwi]]>
<![CDATA[ Anglophile Journal Vol. 3 No. 1 (2023): Anglophile Journal ]]>
Publisher : <![CDATA[CV. Creative Tugu Pena ]]>
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DOI: 10.51278/anglophile.v3i1.481
<![CDATA[The purpose of this study was to put to the test a self-efficacy survey of four language skills (listening, speaking, writing, and reading) using the Jigsaw Learning Strategy. This study was a descriptive qualitative study in which 20 question items about self-efficacy and four language skills in English mastery for health vocational students are tested. This qualitative study was carried out in two classrooms with 39 health vocational students each from January 2021 to August 2022. Two classrooms were set up for the lecture group, one for Jigsaw and one for non-Jigsaw. Self-efficacy questionnaires were used to gather information. In the learning group, there were fourteen sessions totaling two hours, during which each class learned the same practice and comprehension material. Four language skills were worked on by the students. The questionnaire data was analyzed by the product moment to determine validity, and all were found to be valid with results greater than the value of the r table and less than 0.025. The reliability test results on questionnaire data revealed that the data were reliable or consistent. The Keiser-Meyers-Oklin Self-Efficacy Measure in the group of questions for all questionnaire items could be considered appropriate. The Measure of Sampling Adequacy results were also used to determine whether the self-efficacy variable was large enough for further analysis. The communality value was greater than 0.03, indicating a positive relationship between variables. Descriptive analysis, jigsaw classes respond better to self-efficacy surveys than non-jigsaw classes, implying increased self-confidence in the English learning process. Keywords: Jigsaw Learning Strategy, Self-Efficacy English Learning, Validation and Reliability]]>
<![CDATA[The Implementation of Jigsaw Methods in Enhancing English Language Comprehension among Health Polytechnic Students ]]>
<![CDATA[Amelia, Noer]]>;
<![CDATA[Istanto, Wisnu]]>;
<![CDATA[Edi-Krause, Sulistyaning]]>;
<![CDATA[Anggoro, Sapto Dwi]]>;
<![CDATA[Suemith, Merlissa]]>
<![CDATA[ Anglophile Journal Vol. 3 No. 2 (2023): Anglophile Journal ]]>
Publisher : <![CDATA[CV. Creative Tugu Pena ]]>
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DOI: 10.51278/anglophile.v3i2.844
<![CDATA[The purpose of this research is to find out how much students at Health Polytechnic benefit from using Jigsaw techniques to better understand written English. In order to effectively communicate and understand medical principles and procedures, having a strong grasp of the English language is crucial in the healthcare industry. The study looked at how well 78 students from Poltekkes Kemenkes Surabaya learned using a Reading Proficiency test, traditional methods, and pre- and post-test experimental designs. It also used T-tests to look at the results. for a subset of the larger topic, which improves the group's ability to solve problems and make informed decisions. When students from different backgrounds have mastered a subject, they get together to share what they've learned and help each other out. The use of a group strategy not only leads to better overall comprehension, but also to the growth of interpersonal and communicative skills. The purpose of this research is to determine if teaching English using the Jigsaw method is effective for students at Health Polytechnics. The study will rely on statistical methods. Keywords: Jigsaw Method, Reading Proficiency, Cooperative Learning Strategy]]>
