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Luluk Hermawati
Faculty Of Medicine And Health Sciences, Universitas Sultan Ageng Tirtayasa, Serang, Banten, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Calvaria Medical Journal

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Optimalisasi Waktu Fiksasi Menggunakan Formalin 10% Pada Ginjal, Hepar, dan Pankreas Tikus Sprague Dawley Untuk Pewarnaan Haematoxylin-Eosin Alwi, Muhammad Azharan; Rachmawati, Nurlaely Mida; Ariany, Devy; Hermawati, Luluk; Zulfa, Hilizza Awalina
Calvaria Medical Journal Vol 3 No 2 (2025): Edisi Desember 2025
Publisher : Institute for Research and Community Services (LPPM) of University of Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/kp751w77

Abstract

Background: Histotechnology is a series of procedures used to prepare histological specimens for microscopic analysis. One of the critical steps in this process is fixation, which serves to preserve tissue structure and prevent changes in shape or size. However, excessive fixation duration may lead to over-hardening of tissues, resulting in reduced quality of histological observations. Objectives: To evaluate the feasibility of two week formalin 10% fixation period on histological quality of kidney, liver, and pancreas tissues. Methods: After a 14-day adaptation period, male Sprague Dawley rats were anesthetized, necropsied, and their kidney, liver, and pancreas were fixed in 10% formalin. Histotechnical procedures included graded alcohol dehydration, toluene clearing, paraffin embedding, 6 µm sectioning, and HE staining. Preparations were observed using an Olympus BX41 microscope at magnifications of 4x–40x. Results: Two-week fixation caused structural damage to the kidney, liver, and pancreas. Damage observed included glomeruli and tubules in the kidney, the portal triad structure in the liver, and the Langerhans islet cells in the pancreas. Statistical results (if any) should be included here. Conclusions: Fixation for two weeks did not produce adequate histological preparations, and therefore, it is not recommended as a reference for developing histotechnology SOPs in related laboratories.
Co-Authors Abd Rahman Abdul Haris Kuspranoto Abdul Rasyid Adhayati, Baety Agus Sugiharto Agustianti, Erika Agustianti, Erika Ainina Al Shadrina Alwi, Muhammad Azharan Amanah Eva Fidusia Fidusia Ariany, Devy Asma’ul Husna, Firda Awalina Zulfa, Hilizza Awalina, Hilizza Cantika Abadi, Kenny Damayanti , Putri Damayanti, Putri Devy Ariany Dewi, Adinda Puspita Dewi, Lola Febriana Ekawati Rini Wulansari Erfira, Erfira Erika Agustianti Erni Trisnasari Farida, Nida Fathurrohim, Fathurrohim Febriandriani, Dini Fidusia, Amanah Eva Fidusia, Amanah Eva Fitria Mahrunnisa Furqoni , Abdul Hadi Furqoni, Abdul Hadi Ghea Farmaning Thias Putri Herlia, Renata Zhafira Putri Hilizza Awalina Zulfa Hilizza Awalina Zulfa Zulfa Husna, Firda Asma'ul Husna, Firda Asma’ul I Made Arya Subadiyasa Irawati, Nur Bebi Ulfah Ivana Utami, Louisa Kania Pradiva Maharani Kusuma Wardani, Rizky Muchtar Ali Setyo Yudono Muhammad Usman, Muhammad Munifatuzzahra, Munifatuzzahra Muntasiroh, Laily Naldi, Revi Nasution, Silvia Fitrina Neshya Ruriana Putri, Neshya Ruriana Nitalia, Mery Nofisah, Nur Lailatul Nur Bebi Ulfah Irawati Ulfah Irawati Nurlaely Mida Rachmawati Prahanarendra, Galang Pratiwisitha, Maytia Rachmat, Omat Rachmawati, Nurlaely Mida Rifdah Hanifah Rini Wulansa, Ekawati Roslina, Sri Rossa Amelia, Rossa Rukman Abdullah Selfie, Selfie Siti Darifah Siti Darifah Sulthany, Ade Ichwan Susanto, Ratno Utami, Louisa Ivana Wibowo, David Widyawati, Dwi Yuda Nabella Prameswari Yuniarti, Tricahyani Endah Zukhra, Shelly  Zulfa, Hilizza Awalina