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HAYATI Journal of Biosciences
ISSN : 19783019     EISSN : 20864094     DOI : -
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 1,091 Documents
Juvenile Fish Composition and Their Growth Aspects on the East Coast of Banyuwangi, East Java, Indonesia Nazal, Muhammad Faris; Simanjuntak, Charles P.H.; Lumban-Gaol, Jonson; Kurniawan, Fery; Cheung, William W.L.; Reygondeau, Gabriel; Teh, Lydia; Suhita, Ni Putu Asri Ratna
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1185-1198

Abstract

The east coast of Banyuwangi, Bali Strait, has the potential to function as a nursery area for juvenile fish. Therefore, it is essential to research the ecology of juvenile fish resources. This study aimed to uncover the composition of juvenile fishes and their growth aspects through length-weight relationships and condition factors. We collected 9748 fish specimens from floating lift nets during April, September, December 2023, and February 2024. Identification and morphometric analysis were conducted to calculate fish juvenile assemblage composition, growth patterns from length-weight relationships, and condition factors. The study found 48 species from 26 families and 17 orders. The small pelagic fish group was the most common, including species from the Carangidae, Dorosomatidae, Spratelloididae, Engraulidae, Atherinidae, and Scombridae families in terms of the number of individuals and overall biomass. The fish growth on the east coast of Banyuwangi is optimal, with most fish species exhibiting positive allometric and isometric growth patterns. The overall condition factor of the juvenile fish is above 1.00, indicating that the health of fish resources is good. This suggests that the east coast of Banyuwangi, Bali Strait, can support the growth of fish juveniles and maintain the sustainability of fish resources.
Tannase Activity Optimization and Antibiotic Resistance Profiling of Bacteria Isolated from Goat Feces and Ruminal Fluid Mohd Zaki, Nur Farah Syuhada; Aris, Farizan; Mohamed Yunus, Norfatimah; Mat Jalil, Mohd Taufiq; Zakaria, Nurul Aili
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1387-1399

Abstract

Tannase is a vital enzyme produced by microorganisms in the rumen and gastrointestinal tracts of animals, capable of converting tannins—a common anti-nutritional factor in feeds. This study optimized physicochemical conditions of pH, temperature, substrate concentration, and incubation time for evaluating crude tannase activity in tannin-degrading bacteria (TDB) isolated from ruminal fluid (TDB17: Lysinibacillus macroides (KR780381), TDB18: Acinetobacter nosocomialis [MH084921], TDB23: Acinetobacter nosocomialis [MT540255]), and goat feces (TDB24: Acinetobacter nosocomialis [MT540255]). Among these, TDB23: A. nosocomialis (MT540255) demonstrated the highest tannase activity, reaching 96.83 U/ml under optimized conditions. Interestingly, TDB17: L. macroides (KR780381) and TDB24: A. nosocomialis (MT540255) exhibited thermostable tannase across a temperature between 20°C and 80°C, with sustained activity in the range of 60.15-50.34 U/ml and 29.93-28.98 U/ml, respectively. Additionally, the antibiotic resistance profile of these TDB and the synergistic effects of its crude tannase were evaluated using a disc diffusion assay. All TDBs were susceptible to meropenem, tigecycline, gentamicin, streptomycin, and chloramphenicol but resistant to penicillin G, cephalothin, cefoxitin, and vancomycin. Notably, A. nosocomialis (TDB18, TDB23, and TDB24) demonstrated sensitivity to sulfamethoxazole, while L. macroides (TDB17) exhibited resistance. Moreover, the crude tannase synergistically enhanced the antibacterial activity of antibiotics (p<0.05) against both Gram-positive and Gram-negative bacteria.
Genetic External Characteristics of Tukong: Indonesian Rumpless Indigenous Chicken Tribudi, Yuli Arif; Nurgiartiningsih, Veronica Margaretha Ani; Ulfah, Maria; Sari, Aprilianna Putri Zahara Nafsina Luvita; Hariyono, Dwi Nur Happy; Sitaresmi, Pradita Iustitia; Crooijmans, Richard PMA; Natsir, Muhammad Halim; Gusmalawati, Dwi; Ibrahim, Alek
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1147-1156

Abstract

Tukong chicken, also known as rumpless chicken due to the absence of a tailbone, is one of the indigenous chicken breeds in Indonesia currently at risk of extinction. Due to its scarcity, genetic information on the Tukong chicken remains limited, and its potential has not been fully explored. Therefore, this study aimed to determine the genetic external characteristics of the Tukong chickens. Data were collected from 205 chickens (52 males; 153 females) raised in Landak and Bengkayang Regency, West Kalimantan. The varieties are based on feather color, plumage color, feather pattern, feather flicker, comb shape, and shank color. The data were used to analyze the rate of the original gene, the frequency of autosomal genes, sex-linked genes, feather pattern, introgression rate, gene frequency, and expected heterozygosity. The majority of the Tukong chickens had colored feathers (88.78%), columbian feather type (47.80%), plain feather pattern (61.46%), silver feather flicker (54.63%), yellow-white shank (62.44%), and pea comb (77.07%), which were controlled by ii, ee, bb, SS, IdId, and P_ genes, respectively. The introgression rate from exotic breeds into Tukong chicken is 53.2%, with the original gene at 46.8%, while the average expected heterozygosity was 0.416±0.025, indicating a high level of genetic diversity of the traits. This observed variation in qualitative morphological characteristics might benefit both the conservation and utilization of the Tukong chicken.
Diterpene Lactones from Andrographis paniculata and In Vitro Studies on α-Amylase and α-Glucosidase Inhibitory Properties Rachmani, Eka Prasasti Nur; Rehana; Lestari, Puji; Fajriah, Sofa
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1295-1302

Abstract

Plants contain chemical compounds that have medicinal properties. Andrographis paniculata (bitter herb) is commonly used as a traditional medicine, including treating hyperglycemia. A. paniculata was known to contain major compounds, terpenoids, and flavonoids. The phytochemical investigation aimed to isolate and identify chemical compounds from the aerial part of the bitter herb and then evaluate the antihyperglycemic properties. A. paniculata was extracted using 96% ethanol. The extract was partitioned with three organic solvents successively to obtain the chloroform fraction, ethyl acetate fraction, and methanol fraction. Isolation was carried out on the ethyl acetate fraction using column chromatography with a silica stationary phase and an organic solvent mobile phase in various ratios. The isolate in the form of white powder was found in subfraction E8 with the mobile phase chloroform-ethyl acetate (1-2). The structure of the isolated compounds was characterized using spectroscopic methods, including 1D NMR (1H and 13C), 2D NMR (HSQC and HMBC), and LCMS. The isolated compounds were evaluated in vitro with α-amylase and α-glucosidase tests. The results of the data analysis indicated the identification of 14-deoxy-11,12-didehydroandrographolide and 14-deoxyandrographolide as the isolated compounds. The isolated compounds showed α-amylase inhibitory activity with an inhibitory concentration (IC50) of 167.31±4.92 μg/ml and α-glucosidase inhibitory activity with an IC50 of 267.10±6.72 μg/ml. The results of this study indicate that paniculata contains diterpene lactones, which are active in hyperglycemia therapy.
Development of Fermented Coconut Water and Red Ginger using Next Generation Probiotics as a Potential Biological Activity Agent Nabila, Sofi; Srihardyastutie, Arie; Aulanni’am, Aulanni’am; Ardiyati, Tri
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1337-1351

Abstract

Degenerative diseases have increased in prevalence worldwide, predominantly in Indonesia. Some studies found that probiotics have an impact on organic substances that address inflammation and gut microbiota imbalance. This study aims to develop a functional beverage based on coconut water and red ginger fermentation using Next Generation Probiotics (NGP) and characterize the product using metagenomic and metabolomic technologies. This experimental study involved the fermentation of coconut water and red ginger using a probiotic starter identified by 16S rRNA sequencing for 120 hours at room temperature. The analysis included microbial characterization using List the probiotics metagenomic sequencing, metabolite profiling by LC-HRMS, particle size by PSA, and in vitro antibacterial and anti-inflammatory activity testing. Metagenomic analysis identified the dominance of the family Acetobacteraceae (45%) and genus Acetobacter 942%. Fermentation reduced the tannin content by 28.5% and increased the number of metabolite compounds from 37 to 54. The fermentation results also showed antibacterial activity against E. coli CNN 0091 (inhibition zone 7.835 mm), and anti-inflammatory activity reached 1,000%. The combination of coconut water, red ginger, and NGP produced a complex metabolite profile with high therapeutic potential. The findings show significant potential for the prevention of degenerative diseases.
Medium Optimization for Recombinant Human Papillomavirus Type 52 L1 Protein Production in Pichia pastoris GS115 Platform on Bioreactor Scale Mustopa, Apon Zaenal; Nur Amani, Febriyanti; Irawan, Herman; Novianti, Ela; Swasthikawati, Sri; Ekawati, Nurlaili; Nurfatwa, Maritsa; Joko Wahyono, Daniel; Juanssilfero, Ario Betha; Mamangkey, Jendri; Purnomo, Yudi; Hertati, Ai; Wijaya, Hans; Dewi, Kartika Sari; Ningrum, Ratih Asmana
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1283-1294

Abstract

Human papillomavirus (HPV) stands as the primary etiological agent in the development of invasive cervical cancer worldwide. The L1 protein is a pivotal constituent of prophylactic HPV vaccines. Notably, HPV type 52 is one of the most prevalent genotypes found in squamous cell carcinoma cases in Indonesia. This research endeavor aims to enhance the productivity of recombinant HPV-52 L1 protein by optimizing the culture conditions of P. pastoris GS115 cells. In this study, we conducted trials employing 17 different media variants to optimize the expression of recombinant HPV-52 L1 protein. The results from small-scale experiments revealed three media, namely SYN6.10, BMMY, and SYN6.1, which exhibited promising yields of recombinant HPV-52 L1 protein as assessed through ELISA or immunoassay analysis. We succeeded in refining the SYN6.10 derivative, denoted as SYN6.10b, specifically designed for use in 1-L and 5-L bioreactors. This achievement was realized by adjusting Trace Element Solution (TES) and Vitamin Solution (VS) concentrations and implementing a methanol fed-batch phase with the addition of 0.3% methanol after 24 and 48 hours of fermentation in the P. pastoris medium. Further visualizations through SDS-PAGE and western blot analysis confirmed the protein after 72 hours of fermentation in a 1-L bioreactor using the SYN6.10b medium. In conclusion, the SYN6.10b medium required a 72 hours fermentation period to successfully express recombinant HPV-52 L1 protein in the P. pastoris platform.
Species Diversity of Genus Aspergillus, Endophytic Fungal Isolated From Mangrove Ceriops tagal and their Antibiotic Potential Sophia, Anggun; Agustien, Anthoni; Chairul; Syamsuardi
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1174-1184

Abstract

The rapid increase in antimicrobial resistance has encouraged development of new natural and urgent strategies to fight drug-resistant pathogens, one of which is using endophytic fungi. Endophytic fungi found in Ceriops tagal mangrove plant, which can adapt to extreme salinity environments of up to 60 ppt. Endophytic fungi are isolated from leaves, branch, and roots. Each isolate was identified morphologically and cultured in potato dextrose broth media for 21 days. After incubation, liquid culture was extracted with ethyl acetate and evaporated to obtain a thick extract. The Kirby Bauer method was used to evaluate the antibiotic potential of each endophytic fungal extract against pathogenic microbes Staphylococcus aureus, Escherichia coli, and Candida albicans. Concentration used was 5%, antibiotics used as positive controls were chloramphenicol (30μg/disk), nystatin (100 IU/disk), as negative controls DMSO. Three endophytic fungi were isolated from roots (ECT7, ECT 8, ECT 10), two from leaves (ECT 26, ECT 28) and six from branches (ECT 31, ECT 47, ECT 48, ECT 51, ECT 55 and ECT 85). Fungal isolates ECT10 has been proven to effectively exhibit strong antibiotic activity against microorganisms E. coli, S. aureus, and C. albicans, with inhibition zones measuring 13.0 mm, 11.8 mm, and 11.6 mm, respectively.
Metagenomic Analysis of Bacterial Communities in the Musi River Estuary, South Sumatra, Indonesia Melki; Mustopa, Apon Zaenal; Meiyerani, Jeni; Ramadhian, M. Zalfa; Purwiyanto, Anna Ida Sunaryo; Putri, Wike Ayu Eka; Hartoni
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1157-1173

Abstract

Understanding the diversity, function, and ecological dynamics of bacterial communities depends on unique transitional environments, estuaries. Knowledge on bacterial taxa in the Musi River estuary, South Sumatra still eludes, however. This effort aims to identify bacterial species in estuary waters using metagenomic analysis based on 16S rRNA gene. Surface water samples from three study locations were analysed by DNA isolation, nanodrop spectrophotometer qualitative assessment, 16S rRNA gene amplification, electrophoresis, and Illumina NovaSeq sequencing. Results showed that Proteobacteria predominated at all sites, followed by Campilobacterota, Cyanobacteria, and Bacteroidota. At the class level, Gammaproteobacteria was most common, followed by Alphaproteobacteria and Campylobacteria. Dominant bacterial orders were Campylobacterales, Rhodobacterales, and Pseudomonadales while the most common families were Arcobacteraceae, Rhodobacteraceae, and Pseudomonadaceae. The most plentiful genera were Rheinheimera, Pseudomonas, and Pseudarcobacter. Variations in bacterial spread among stations suggest environmental factors including salinity, nutrient availability, and human activities influencing microbial community composition. Ternary plots, heat maps, and krona diagrams were employed to disclose distinct patterns of bacterial community dispersion in the estuary. This paper underscores the importance of metagenomic research in illuminating microbial diversity in estuarine environments and its impact on ecological dynamics and water quality.
Evaluating the Repellent Efficacy of a Vitex trifolia L. Leaf Powder Botanical Formulation Against Oriental Fruit Flies (Bactrocera dorsalis) Ainun, Andi Dzul Arsyi; Sjam, Sylvia; Sulaeha, Sulaeha; Fitriyanti, Nur Azizah
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1251-1259

Abstract

Fruit flies (Diptera: Tephritidae) are important horticultural crop pests that cause significant losses in both crop quality and quantity. Vitex trifolia L., often considered a weed, contains bioactive compounds with potential as fruit fly repellents. This research aims to evaluate the repellent activity of V. trifolia against oriental fruit flies (Bactrocera dorsalis) at various concentrations. Laboratory experiments were conducted using a completely randomized design (CRD) with four treatments (1%, 3%, 5%) and one control, each repeated five times, Testing was carried out using an olfactometer. The results showed that all tested concentrations significantly reduced B. dorsalis visits compared to the control (0%), with the highest repellency (65.83%) observed at the 5% concentration. A negative linear relationship was established between formulation concentration and B. dorsalis activity, including visits, ovipositor punctures, and larval presence. The formulation significantly decreased oviposition punctures and larval infestation, resulting in a lower percentage of infested fruits (28.57% at 5% concentration compared to 82.86% in the control). Physical and organoleptic assessments confirmed the formulation's suitability for practical application, with a moisture content of (2.66%), a light green color, and a distinctive spicy aroma. Gas Chromatography–Mass Spectrometry (GC-MS) analysis identified key bioactive compounds, including phenol, methyl β-D-glucopyranoside, vitexifolin D, and viteosin A, which are known for their repellent properties. These findings suggest that V. trifolia leaf powder is a promising, eco-friendly alternative for managing B. dorsalis infestations in horticultural crops.
Spatial Variability of Water and Sediment Quality in Pond Outlet: Implications for Coastal Ecosystems in Mangrove Areas, Pasuruan, East Java Rizky, Putri Nurhanida; Halim, Atika Marisa; Nisa, Andina Chairun; Prama, Ega Aditya; Ulfauza; Oktahimawan, Muhammad Khusni
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1313-1323

Abstract

Aquaculture expansion in Pasuruan's coastal areas raises concerns about water and sediment pollution, but the spatial variability and magnitude of these impacts remain insufficiently quantified. This study aimed to assess the water and sediment quality at pond outlets across different aquaculture systems. Samples were collected from seven intensive (high-input shrimp farming), traditional (low-input, extensive ponds), and silvofishery (integrated aquaculture with mangroves) aquaculture farms from July to September 2024, coinciding with peak farming activity. Water and sediment samples were collected from seven farms and analyzed for chemical parameters, organic matter content, and redox potential. Results of the current research record extensive spatial and temporal heterogeneity that is driven by aquaculture management and external events like the WSSV epizootic in August 2024. Ammonia concentrations increased dramatically at stations within intensive vannamei shrimp farms (5.5 mg/L), while downstream stations exhibited natural dilution and mitigation by mangroves. Sediment quality analysis demonstrated a reducing condition with redox potential values from as low as -100 mV and SOM concentrations as high as 23%. Correlation analysis highlighted intensive farming systems as the main drivers of water and sediment degradation, with mangrove belts demonstrating resistance through nutrient adsorption and filtration with COD, TSS, and organic matter as primary pollution contributors. These findings emphasize the urgent need for adopting sustainable practices, such as polyculture systems, reduced feed input strategies, the implementation of constructed wetlands, and enhanced mangrove rehabilitation around aquaculture zones, to minimize environmental impacts and preserve coastal ecosystem health.

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