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All Journal Jurnal Akuakultur Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences Journal of Environmental Engineering and Sustainable Technology AJIE (Asian Journal of Innovation and Entrepreneurship) Jurnal Akuakultur Sungai dan Danau Indonesian Aquaculture Journal Jurnal Riset Akuakultur CARADDE: Jurnal Pengabdian Kepada Masyarakat Journal of Aquaculture and Fish Health Dinamika Journal : Pengabdian Masyarakat Jurnal Abdi Insani Buletin Poltanesa Jurnal MAIYAH Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Lempuk: Jurnal Ilmu Kelautan dan Perikanan Journal of Artha Biological Engineering Artha Imperium: Jurnal Pengabdian Kepada Masyarakat
Soedibya, Petrus Hary Tjahja
Fakultas Perikanan Dan Ilmu Kelautan Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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GROWTH PERFORMANCE AND INTESTINAL Aeromonas spp. OF BONYLIP BARB (Osteochilus vittatus) AFTER RECEIVING DIET CONTAINING SALT AND HERBAL CONCOCTION SUPPLEMENTATION Syakuri, Hamdan; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Ekasanti, Anandita; Kasprijo, Kasprijo; Kusuma, Rima Oktavia; Wijaya, Rudy; Nugrayani, Dewi; Listiowati, Emyliana; Palupi, Mustika
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.111-122

Abstract

Salt and herbal supplementations in fish feed are expected to promote aquaculture productivity, including for slow-growing fish such as Bonylip barb (Osteochilus vittatus). The objective of this study was to evaluate the growth performance and occurrence of Aeromonas spp. in the intestine of Bonylip barb fed a diet supplemented with salt and herbs. This experiment was conducted using a completely randomized design with four treatments: 1) control, 2) salt 3% supplementation, 3) herbal supplementation, and 4) Salt 3% and herbal supplementation. Growth performance evaluation included weight gain, relative growth rate, and specific growth rate after 30 and 60 days of rearing.  Bacterial samples were isolated on trypticase soy agar (TSA) and glutamate starch phenol red agar (GSP) media. Yellow colonies on GSP were counted and confirmed as Aeromonas spp. via molecular identification based on 16S rDNA sequence. Results showed that herbal supplementation, either alone or in combination with 3% salt, insignificantly increased the fish growth performance in the short-term (30 days), but tended to decrease it over the long-term (60 days). Salt supplementation consistently decreased fish growth performance but improved fish survival rates. Aeromonas isolates comprised more than 60 % of the culturable intestinal bacteria in all treatments. Furthermore, herbal supplementation appeared to increase the percentage of Aeromonas spp. in the intestine, with identified species including Aeromonas veronii, Aeromonas caviae, and Aeromonas jandaei.
ISOLASI, IDENTIFIKASI, DAN ANALISIS EKSPRESI GEN PENGKODE GROWTH HORMONE PADA IKAN SIDAT (Anguilla bicolor) Syakuri, Hamdan; Wibowo, Satrio Haryu; Ekasanti, Anandita; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Listiowati, Emyliana; Nugrayani, Dewi; Sukardi, Purnama
Jurnal Riset Akuakultur Vol 19, No 1 (2024): (Maret 2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.1.2024.69-83

Abstract

Gen hormon pertumbuhan (growth hormone, GH) perlu dipelajari untuk mendukung domestikasi ikan sidat Anguilla bicolor di Indonesia. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi gen pengkode GH serta menganalisis ekspresinya pada sampel ikan sidat A. bicolor. Primer untuk amplifikasi gen GH ikan sidat A. bicolor didesain berdasarkan sekuen gen GH dari beberapa spesies ikan sidat lain yang tersedia di GenBank. Sampel cDNA hipofisa dan otak ikan sidat digunakan untuk amplifikasi gen GH. Hasil amplifikasi disekuensing dan hasilnya dianalisis menggunakan analisis BLAST, multiple sequences alignment, hormon signature, dan filogenetik. Analisis ekspresi gen GH dilakukan menggunakan teknik realtime PCR dengan metode delta-delta CT pada 14 sampel ikan sidat (23,1 ± 19,6 g; 24,5 ± 4,2 cm). Fragmen DNA sepanjang 486 bp berhasil diamplifikasi dan disekuensing. Sekuen gen GH A. bicolor memiliki similaritas nukleotida sebesar 98,49-99,14% jika dibandingkan dengan gen GH ikan sidat A. anguilla, A. australis, dan A. japonica. Sekuen parsial tersebut secara in silico diketahui mengkode bagian dari protein GH sepanjang 155 asam amino (aa). Sekuen asam amino protein GH A. bicolor sangat mirip dengan sekuen spesies ikan sidat lain dengan hanya tiga perbedaan asam amino dan membentuk satu percabangan pada pohon filogenetik. Tingkat ekspresi gen GH pada sampel ikan sidat memiliki variasi yang tinggi. Hasil penelitian ini dapat menjadi dasar untuk studi selanjutnya khususnya berkaitan dengan peran gen GH dalam pertumbuhan ikan sidat A. bicolor.The growth hormone (GH) gene needs to be studied to support the domestication of the Anguilla bicolor eel in Indonesia. This study aimed to isolate and identify the GH coding gene and analyze its expression in samples of the eel A. bicolor. Primers for amplification of the GH gene of A. bicolor eels were designed based on GH gene sequences from several other eel species available in GenBank. Pituitary and brain cDNA samples of eel were used for GH gene amplification. The amplification results were sequenced and the results were analyzed using BLAST analysis, multiple sequence alignment, hormone signature, and phylogenetic analysis. The GH gene expression analysis was carried out using the real-time PCR technique with the delta-delta CT method on 14 eel samples (23.1 ± 19.6 g; 24.5 ± 4.2 cm). The 486 bp DNA fragment was successfully amplified and sequenced. The GH gene sequence of A. bicolor has a nucleotide similarity of 98.49-99.14% when compared with the GH gene of other eel species A. anguilla, A. australis, and A. japonica. This partial sequence was found in silico to code 155 amino acids (aa) GH protein. The amino acid sequence of the A. bicolor GH protein is very similar to that of other eel species with only three amino acid differences and forms one branch on the phylogenetic tree. The expression level of the GH gene in eel samples had high variations. The results of this study could be a basis for further studies, especially regarding the role of the GH gene in the growth of the eel A. bicolor. 
Penggunaan infusum daun ketapang (Terminalia catappa) sebagai bahan anestesi terhadap diferensiasi leukosit dan waktu induktif sedatif ikan nila (Oreochromis niloticus) Luthfiannisa, Aisyah; Hary Tjahja Soedibya, Petrus; Pramono, Taufik Budhi
Lempuk: Jurnal Ilmu Kelautan dan Perikanan Vol. 3 No. 1 (2024): Juni
Publisher : Fishery Product Technology Study Program, Yudharta University, Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/lempuk.v3i1.5453

Abstract

Tilapia is one of the leading commodities in fisheries and requires proper handling in the distribution process so that fish do not experience stress and death. One of them is by using anesthesia. Anesthetic ingredients are divided into two, namely natural and artificial, one of the natural ingredients that has the potential as an anesthetic is ketapang leaves because it has flavanoid and alkaloid compounds that are considered capable of providing hypnotic sedative effects and anti-anxiety substances for fish. The study applied 4 treatments (5 ppm; 10 ppm; 15 ppm; and 20 ppm) of ketapang leaf infusum solution with the method used was descriptive qualitative to obtain research conclusions. The results obtained were the use of ketapang leaf infusion was able to increase lymphocyte levels from 42.13-58.15% to 52.35-60.21%. At a concentration of 20 ppm ketapang leaf infusion has the fastest inductive time of 23 minutes 41 seconds with a sedative time of 18 minutes 52 seconds. From these results it can be concluded that ketapang leaf infusion has potential as a natural anesthetic, but other studies are still needed regarding the percentage of content, effective dose and toxicity of ketapang leaf infusion solution on the hematological condition of tilapia.
Effect of Additional Dried Tubifex sp. in Commercial Feed Against Color Intensity of Guppy (Poecilia reticulata) Nurjihan, Haniah Istiqomah; Kasprijo, Kasprijo; Muslih, Muslih; Soedibya, Petrus Hary Tjahja; Fitriadi, Ren; Esa, Yuzine
Journal of Aquaculture and Fish Health Vol. 13 No. 2 (2024): JAFH Vol. 13 No. 2 June 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i2.39061

Abstract

Guppy fish (Poecilia reticulata) is a type of ornamental fish that is in great demand, because of its small size and beautiful color. The color of ornamental fish will generally fade, due to a lack of carotenoids in their feed. The purpose of this study was to determine the effect of giving Tubifex sp. in commercial feed to increase the color intensity of guppy fish and the best dose of Tubifex sp. The test fish used were male guppy fish strain HB Red. This study used a completely randomized design (CRD) method with five treatments and three replications, that is commercial feed with the addition of Tubifex sp. with doses of 0% (P1), 2% (P2), 4% (P3), 6% (P4), and 8% (P5). Parameters observed were color intensity (chroma value), survival, and water quality. Addition of Tubifex sp. in commercial feed gave the effect with the highest yield on P5 at a dose of 8%, with an increase in color intensity (chroma value) of 4,21±0,25d. At P1 it gave an increase of 1,19±0,02a, P2 was 1,34±0,04a, P3 was 1,81±0,21b, and P4 was 2,88±0,18c. So, the best treatment is P5 (8%). Survival showed the results were not significantly different, that is 100%. Water quality is included in the tolerance limit of fish with the results of temperature 26,6 – 27,4°C, pH 7,4 – 7,9, and DO 6,2 – 7,7 mg/L.
The Effect of Cultivation Techniques in The Dry and Rainy Seasons on The Quality of The Seaweed Caulerpa racemosa Darmawati, Darmawati; Soedibya, Petrus Hary Tjahja; Anwar, Asni; Murni, Murni; Tang, Beddu
Journal of Aquaculture and Fish Health Vol. 13 No. 3 (2024): JAFH Vol. 13 No. 3 September 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i3.54784

Abstract

The seaweed Caulerpa racemosa has great potential to be developed because it has economic value and potential as a functional food. This seaweed contains nutrients, including protein, carbohydrates, polyunsaturated fatty acids, fiber minerals, and antioxidants. The availability of seaweed is still very dependent on nature and has not been appropriately cultivated. The planting season and appropriate cultivation methods will influence seaweed's quality/nutritional content. This research aims to analyze the nutritional content of seaweed C. racemosa cultivated in different growing seasons. The research method used a floating raft system with a completely randomized design and a factorial pattern carried out in two seasons, namely the dry and rainy seasons. Proximate analysis was carried out to determine the nutritional composition of seaweed C. racemosa, including protein, crude fiber, water, ash, fat, and carbohydrate (NFE). Next, it was analyzed using ANOVA with a further test with Tukey. The research results showed differences in the nutritional composition of seaweed C. racemosa from each treatment cultivated in the dry and rainy seasons. The nutritional composition of seaweed C. racemosa best treatment is found in the plant spacing of 30 cm with a depth of 50 cm in the dry season with the nutritional content namely protein (13.8%), NFE (20.75%), crude fiber (15.71%), a fluctuating fat content of 0.16-1.42%, highest ash (34.69%), water content (35%), moderate in the rainy season namely: protein (10.8%), BETN (10.78%), crude fiber (12.33%), fat content fluctuates (0.18-1.06%), highest ash (30.21%), water content (41.01%).
Activity of Cellulolytic Bacteria Isolated From The Digestive Tract Of Jerbung Shrimp (Feneus marguensis) Fitriadi, Ren; Sabdaningsih, Aninditia; Budi Prayitno, Slamet; Soedibya, Petrus Hary Tjahja; Sarjito, Sarjito; Subagiyo, Subagiyo
Journal of Aquaculture and Fish Health Vol. 14 No. 1 (2025): JAFH Vol. 14 No. 1 February 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i1.66897

Abstract

Bacteria found in the digestive tract of shrimp mostly play a major role in nutrient absorption. One of the nutritional contents that is quite difficult for shrimp to absorb is fiber. Bacteria that can degrade fiber content are known as cellulolytic bacteria. This study aimed to determine the activity index of cellulolytic bacteria found in the digestive tract of shrimp. The research method includes sampling shrimp with three different categories based on average length and weight, divided into three categories such as large (22.2 g ± 87.3 cm), medium (17.1 g ± 36.7 cm), and small (13.4 g ± 18.8 cm) taken from the north coast waters of Pemalang and the south coast waters of Cilacap. Furthermore, bacterial isolation, morphological observation, calculation of bacterial abundance, and testing of cellulolytic activity were carried out. The results showed that 150 bacteria were successfully isolated from both locations at different sizes and a total of 68 bacterial isolates had cellulolytic activity. There were 22 potential isolates from each coastal water that had a great cellulolytic activity index, ranging from 1.4 to 7.0. Moreover, the most potential activities were performed by 8 bacterial isolates encoded with GM.1, GM.2, GM.3, GM.4, GM.5, GM.6, GM.7, and GM.8 with a range of cellulolytic index obtained ranging from 3.0-7.0. The high activity index of the cellulolytic bacteria obtained is a fairly promising potential as a probiotic candidate that plays a role in degrading fiber in food found in the digestive tract of shrimp so that it can accelerate its growth.
Co-Authors Adinda Kurnia Putri Amalia Khoerunnisa, faza Aminuddin, Jamrud Aninditia Sabdaningsih Anwar, Asni Arif Mahdiana Darmawati Darmawati Deandra Aurel Azeli Desy Ratnaningsih, Navita Dewi Nugrayani Ekasanti, Anandita Emyliana Listiowati Emyliana Listiowati, Emyliana Esa, Yuzine Faza Fikriyya, Nabela Fitriadi, Ren Gyogyo, Iriyama Hastuti, Dewi Wisudyanti Budi Heri Ariadi Irwan Hidayatullah, Irwan Isdy Sulistyo Jefri Anjaini Kasprijo Kasprijo, Kasprijo Kusuma, Rima Oktavia Lilik Kartika Sari Linayati, Linayati Liu, Chun-Hung Luthfiannisa, Aisyah Marhaendro Santoso Muhammad Riady, Reza Murni Murni Muslih Muslih Mustika Palupi Nugrayani, Dewi Nuning Vita Hidayati Nurhafid, Mohammad Nurjihan, Haniah Istiqomah Palupi, Mustika Purnama Sukardi Purnama Sukardi Purnama Sukardi Putri, Desta Fatma Raharja, Ferry Cahya Rattanavichai, Wutti Ren Fitriadi Rika Prihati Cahyaning Pertiwi Riviani Riviani Riviani, Riviani Rosi Widarawati RR. Ella Evrita Hestiandari Saputra, Dian Rizqi Sarjito - Shokib, Maskhiyatus slamet budi prayitno Sri Marnani Sri Marnani Sri Marnani Sri, Marnani Subagiyo Subagiyo Sukiyati, Eny Sutanto Syakuri, Hamdan Tang, Beddu Taufik Budhi Pramono Taufik Budhi Pramono Taufik Budhi Pramono Taufik Budhi Pramono Tohap Simangunsong Tohir Tohir, Tohir Tri Yusufi Mardiana Trianingtyas, Laela Vita, Tika Ayunda Wibowo, Mahbub Fuad Wibowo, Satrio Haryu widianti, lia Wijaya, Rudy