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Journal : ovozoa journal of animal reproduction

Profil bakteri non spesifik dalam lendir serviks sapi perah pada fase folikuler dan fase luteal Nur, Maysarah Oktavia; Mulyati, Sri; Sardjito, Trilas; Chusniati, Sri; Tyasningsih, Wiwiek; Mafruchati, Maslichah
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (830.154 KB) | DOI: 10.20473/ovz.v9i1.2020.17-22

Abstract

This research was conducted to identify non-specific bacteria found in the cervical mucus of dairy cattle in the follicular and luteal phase. Twenty cervical mucus samples were collected from ten cows during their follicular phase and ten cows during their luteal phase. Sampling was carried out with the help of an artificial insemination gun and a plastic sheath, which were protected by a sheath cover. After insertion into cervix, the tip of the plastic sheath was cut and put into a tube prefilled with phosphate buffered saline. All samples were cultured in Blood Agar media and isolated in Eosin Methylene Blue Agar, Mac Conkey Agar, and Manitol Salt Agar. Gram staining was applied to distinguish gram-positive and gram-negative bacteria; and followed with the catalase, spore, motility, and biochemical tests to further identify bacteria. The bacteria identified were of the genus Enterobacter, Staphylococcus, Corynebacterium, Streptococcus, Escherichia, and Pasteurella. It could be concluded that non-specific bacteria of the same genus were identified in cervical mucus of dairy cows in their follicular and luteal phase, in a various of percentages.
Co-Authors Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Amal, Mohammad Noor Azmai Amelia, Ezanti Nur Amrullah, Amirul Muslim Baroroh, Diana Nova Boedi Setiawan Bura, Maria Antonia Yersi Dua Chusniati, Sri Dadik Rahardjo, Dadik Darmawan, Moch. Rizky Dwi, Welyanto Kurniawan Erma Safitri Estoepangestie, Agnes Theresia Soelih Fadlilah, Shinta Levea Ni'matul Faisal Fikri Fedik Abdul Rantam Hamid, Iwan Sharial Hana Eliyani Hanifa, Muril Hizriyana Harijani, Nenny Hayati, Laila Nur Hendarti, Gracia Angelina Indah Purnamasari Indriyani, Dwi Putri Iwan Sahrial Hamid Kadek Rachmawati Khairullah, Aswin Rafif Khanand, Nadeem Khasana, Uswatun Kuncorojati, Suryo Kusuma, Agung Jati Kusumastuti, Imbi Legowo, Djoko Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Meles, Dewa Ketut Murjianingsih, Fitriani Mustofa Helmi Effendi Nur, Maysarah Oktavia Nurwartanti, Maya Perdana, Benny Permatasari, Dian Ayu Poetranto, Emmanuel Djoko Praja, Ratih Novita Prameswari, Ratna Ajeng Pratama, Dio Putra Rahadyan, Raihan Alif Raharjo, Dadik Rahmahani, Jola Ramandinianto, Sancaka Chasyer Rochmah Kurnijasanti Rozi Salleh, Annas Saputro, Amung Logam Sari, Putri Desi Wulan Sarudji, Suryanie Soeharsono Soeharsono Sri Mulyati Suwarno Suwarno Suwarno Tacharina, Martia Rani Tehupuring, Benjamin Chr. Tiara Wandira Hariyanto Trilas Sardjito Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Wijaya, Achmad Arby Wiwik Misaco Yuniarti Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwantanti Yunita, Maya Nurwartanti Yunus , Muchammad