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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan journal of Basic Medical Veterinary Current Biomedicine
Tyasningsih, Wiwiek
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : journal of Basic Medical Veterinary

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Pengaruh Aflatoksin 9,58 ppb dalam Pakan Terhadap Biometrik Saluran Cerna dan Berat Badan Ayam Petelur Periode Awal Pertumbuhan Pratama, Dio Putra; Tehupuring, Benjamin Chr.; Poetranto, Emmanuel Djoko; Eliyani, Hana; Soeharsono, Soeharsono; Tyasningsih, Wiwiek; Hendarti, Gracia Angelina
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28585

Abstract

The purpose of this study was to determine the effect of aflatoxin content of 9.58 ppb in feed on gastrointestinal biometrics and weight gain of laying hens in the early growth period. Thirty-six day old chicks (DOC) were used in this study and were divided into two groups P0 and P1. P0 (control group) was fed with 100% basal diet and P1 (treatment group) was fed a diet with a composition of 80% basal feed + 20% aflatoxin-containing feed. After the DOC was adapted, the first feeding containing aflatoxin was given from the 20th day to the 60th day. Data collection was carried out three times, namely the 20th, 40th, and 60th days. At the end of each period, the chickens would be euthanized to collect data on the weight and length of the digestive tract and body weight. Weight gain with time increased, but had no significant effect (p>0.05) between the control and treatment groups. Body weight continues to increase because the body's homeresis function is still running well. Observations on gastrointestinal biometrics showed significant differences (p<0.05) in oesophagus weight, proventriculus weight, ventriculus weight, jejunum weight, ileum weight and length, and cecum weight because aflatoxins have a direct effect on the digestive tract they pass. The conclusion of this study was that the provision of 20% aflatoxin-containing feed did not affect weight gain but had shown an effect on the biometrics of several gastrointestinal tracts.
Detection of eae Gene as A Marker Strain EPEC (Enteropathogenic Escherichia coli) in Chicken Meat Sold at Some Traditional Markets in Surabaya Amrullah, Amirul Muslim; Rantam, Fedik Abdul; Raharjo, Dadik; Tyasningsih, Wiwiek; Estoepangestie, Agnes Theresia Soelih; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.31134

Abstract

This study aimed to detect the presence of eae gene in E. coli from chicken meat sold at the traditional market Surabaya. Identification of bacteria was carried out in several stages, inoculated on pre-enrichment media using Buffered Peptone Water (BPW), planting on Levine's Eosin Methylene Blue Agar (L-EMB) media, suspected colonies on L-EMB were subjected to Triple Sugar Iron Agar (TSIA) and Indol test for the confirmation of E. coli. The confirmed E. coli isolates were tested by Polymerase chain reaction (PCR) to determine the presence of the eae gene. The results showed that E. coli could be identified in 28 out of 100 (28%) chicken meat samples, and eae gene was detected in three E. coli isolates (11%).
Acute Toxicity Test Of The Green Viper Snake (Trimeresurus albolabris), Macroscopic Description Of The Kidney And Liver Of Mice (Mus musculus) Darmawan, Moch. Rizky; Rahardjo, Dadik; Tyasningsih, Wiwiek; Kurnijasanti, Rochmah; Legowo, Djoko; Setiawan, Boedi
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31135

Abstract

According to WHO (2010), the Trimeresurus albolabris snake is a venomous snake with a high number of bite cases in Indonesia. Information on the character and level of toxicity of snake venom in Indonesia, including Trimeresurus spp. snakes, is still minimal. This study aims to determine the LD50 value (lethal dose 50), clinical symptoms, and to determine the macroscopic appearance of the liver and kidneys of mice (Mus musculus) after being injected by a the white-lipped pit viper (Trimeresurus albolabris) venom. The LD50 value of Trimeresurus albolabris venom injected subcutaneously into mice was 5,09 mg/kgBW. Clinical symptoms that appear increased movement activity, increased respiratory rate, lethargy, decreased activity, and reduced grooming. Macroscopic appearance of the liver that looks abnormal is found (1) color changes in the form of pale, pale yellowish, and red spots (petechiae). (2) changes in surface structure found only one liver that is not flat. (3) changes in consistency in the form of brittle easily. In the kidney that looks abnormal, found (1) changes in color in the form of pale. (2) changes in surface structure were only found in one kidney in the form of nodules. (3) changes in consistency in the form of brittle easily and soft.
Co-Authors Amelia, Ezanti Nur Amrullah, Amirul Muslim Baroroh, Diana Nova Boedi Setiawan Chusniati, Sri Dadik Rahardjo, Dadik Darmawan, Moch. Rizky Dua Bura, Maria Antonia Yersi Dwi, Welyanto Kurniawan Erma Safitri Estoepangestie, Agnes Theresia Soelih Fadlilah, Shinta Levea Ni'matul Faisal Fikri Fedik Abdul Rantam Hamid, Iwan Sharial Hana Eliyani Hanifa, Muril Hizriyana Harijani, Nenny Hayati, Laila Nur Hendarti, Gracia Angelina Indah Purnamasari Indriyani, Dwi Putri Iwan Sahrial Hamid Kadek Rachmawati Khairullah, Aswin Rafif Khasana, Uswatun Kusuma, Agung Jati Kusumastuti, Imbi Legowo, Djoko Lucia Tri Suwanti, Lucia Tri Meles, Dewa Ketut Murjianingsih, Fitriani Mustofa Helmi Effendi Nurwartanti, Maya Permatasari, Dian Ayu Poetranto, Emmanuel Djoko Praja, Ratih Novita Prameswari, Ratna Ajeng Pratama, Dio Putra Rahadyan, Raihan Alif Raharjo, Dadik Rahmahani, Jola Ramandinianto, Sancaka Chasyer Rochmah Kurnijasanti Saputro, Amung Logam Sarudji, Suryanie Soeharsono Soeharsono Suwarno Suwarno Tacharina, Martia Rani Tehupuring, Benjamin Chr. Tiara Wandira Hariyanto Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Wijaya, Achmad Arby Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwantanti Yunita, Maya Nurwartanti