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All Journal Bioscience Jurnal Pengajaran MIPA Biogenesis: Jurnal Ilmiah Biologi BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pendidikan IPA (JPPIPA) BIOEDUKASI Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology BIOEDUSCIENCE Jurnal Pengabdian Masyarakat AbdiMas Indonesian Journal of Biotechnology and Biodiversity Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Jurnal Kreativitas PKM Manuju : Malahayati Nursing Journal Jurnal Dedikasi Pendidikan Poltekita : Jurnal Ilmu Kesehatan Journal of Appropriate Technology for Community Services Jurnal Abdi Insani Jurnal Marketing Nusantara Science and Technology Proceedings Jurnal Pengabdian UNDIKMA Jurnal Pengabdian Nasional (JPN) Indonesia Jurnal Pengabdian kepada Masyarakat JURNAL STUDIA KOMUNIKA KATALIS: Jurnal Penelitian Kimia dan Pendidikan Kimia DIKSAINS : Jurnal Ilmiah Pendidikan Sains Jurnal Locus Penelitian dan Pengabdian Muhafadzah: Jurnal Ilmiah Bimbingan dan Konseling Islam RANGGUK: Jurnal Pengabdian Kepada Masyarakat
Seprianto, Seprianto
Program Studi Bioteknologi, Fakultas Ilmu-ilmu Kesehatan, Universitas Esa Unggul Jl. Arjuna Utara No. 09 Kebun Jeruk, Jakarta 11510
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : BIOEDUKASI

 1 
Optimization of Real-Time PCR Conditions for COVID-19 Diagnosis with Logix Smart Reagent™ Anisa Febriyanti; Seprianto Seprianto; Titta Novianti; Febriana Dwi Wahyuni; Oktaviani Naulita Turnip; Roselein Putri; Henny Saraswati
BIOEDUKASI Vol 20 No 1 (2022)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v20i1.28356

Abstract

Corona Virus Disease 2019 or COVID-19 is a new type of virus that attacks the respiratory tract and can cause death. Laboratory examinations play an essential role in diagnosing COVID-19 with a set of reagents or kits. Sampiand sampling is carried out with a nasopharyngeal swab or oropharyngeal swab. Positive samples of COVID-19 patients used in this study were converted into RNA at the COVID-19 Referral Clinic in Bekasi, after which volume optimization was carried out with a total volume of 5 µl, 8 µl, and 10 µl with the Logix Smart™ kit. The method in this study uses One-Step Real-time PCR. This method is the best method for carrying out several bear tests because it can reduce the possibility of sample contamination. The procedure is fast and has high sensitivity. The fluorescence detection used in this study was FAM with a specific target of COVID-19 RNA and ROX with a particular DNA target of RNase-P. This research was conducted to obtain optimal volume conditions under the manufacturer's standards in detecting the SARS-CoV-2 virus. The results of this study indicate that a total volume of 5 l is the optimal total volume for detecting the presence of the SARS- CoV-2 virus in samples taken from patients.
Co-Authors Abda Abda Adelia, Ismi Agustinus Robert Uria alfero Putra Iryanto Alham, Fiddini Anisa Febriyanti Ariyadi, Noprizal Aroem Naroeni Ayu, Yulia Agustina Azwar, Beni callista marsya Chikita, Daien Cyndi Prasetya Daflaini, Daflaini Darwata, Siti Riva DEWI PURNAMA Dewi Seswita Zilda Dewi Seswita Zilda Dewi, Lisa Rahma Dewi, Mustika Sandra Dina Hajja Ristianti, Dina Hajja Effendi, Desy Irafadillah Erani, Friska Fadila Fadila, Fadila Fajariyanto, Gunawan Febriana Dwi Wahyuni feby feby Feliatra Gloria, Meilina Gusvina, Fadilla Hana Nurullita Prestisia Harna, Harna Hasibuan, Molani Paulina Henny Saraswati Henny Saraswati Idi Warsah Irfandi, Ahmad Jofrishal Jofrishal Juniyati, Sintia Kevin Febrianus Moda Kibtiyah, Maryatul Lawrence, Yoel Lesmawan, Hendra Mahyuny, Siska Rita Manalu, Mely Grace Manalu Maulida, Maulida Mauliza, Mauliza Muhamad Amza Sidiq Muhammad Arreza nie nie Noer Khasanah Noperta, Noperta Nora, Adri Novianti, Titta Nugraha, Jaka Setia Nurmalasari, Mieke Nursanah, Nursanah Ogi Danika Pranata Oksal, Efriyana Oktaviani Naulita Turnip Oktaviani, Coryna PUSPITA LISDIYANTI Puspita Lisdiyanti PUTRI HANDAYANI Putri Handayani putri mega utami Putri, Mentari Darma Rahmah, Rasya Aulia Rahmayanti R, Andi Riansyah, Irza Rini Hidayati roaslein putri Roaslein Putri Roselein Putri Ruhmayanti, Nur Ayu Sa'pang, Mertien Sabar Pambudi Saraswati, Henny Saraswati, Henny Sari, Ratih Permana Seda, Ananda Putri Sianturi, Dody Jhonatan Wilfiez Sidauruk, Christina Siti Komariyah Situmorang, Larissa Kristina T.T. Soleha, Sovatunisa Suharsono Suharsono Surgasari, Tania Susanti, Sela Syamsul Rizal Titania Tjandrawati Nugroho Veza Azteria Widodo Widodo Wiriatmaja, Nuraini Ulya Yusro Nuri Fawzya Yusro Nuri Fawzya Zadrax, Adellino Zhafira, Alipya