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Seprianto, Seprianto
Program Studi Bioteknologi, Fakultas Ilmu-ilmu Kesehatan, Universitas Esa Unggul Jl. Arjuna Utara No. 09 Kebun Jeruk, Jakarta 11510
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : BIOEDUSCIENCE

 1 
Optimasi Volume Kit Da An Gene Untuk Deteksi SARS-CoV-2 dengan Real Time RT-PCR Seprianto; Muhammad Arreza; Titta Novianti; Febriana Dwi Wahyuni; Oktaviani Naulita Turnip; Roaslein Putri; Henny Saraswati
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (566.732 KB) | DOI: 10.22236/j.bes/628595

Abstract

Background: SARS-CoV-2 is a new type of coronavirus of the genus Betacoronavirus and the family Coronaviridae that causes a respiratory disease called COVID-19. The virus has a sheath and genetic material in the form of single-chain RNA. The genome structure of this virus is divided into two types, namely genes that encode non-structural proteins consisting of the ORF1a / ORF1b gene and genes that encode structural proteins consisting of spike glycoprotein (S), envelope (E), membrane glycoprotein (M), and nucleocapsid protein (N). Methods: The method of detecting SARS-CoV-2 with real time RT-PCR is the most recommended method because it has high specificity and accuracy. The specificity of a method is necessary to be able to specifically recognize the pathogen that causes the disease. Real time RT-PCR requires sampling with a swab on the oropharynx or nasopharynx to be examined in the laboratory which later the presence of viral RNA becomes a molecule that is assessed for diagnosis results. In this study, volume optimization was carried out on the Da An Gene kit used for the detection of SARS-CoV-2 with Reverse Transcription Polymerase Chain Reaction (Real time RT-PCR) with the aim of saving the use of reagents from available kits but with amplification results remaining optimal and accurate. Results: There were three SARS-CoV-2 RNA samples used consisting of N62, N63, and N79 samples and three types of total volume used were 20 μl, 15 μl, and 10 μl. The results of this study showed that the three positive samples contained SARS-CoV-2 with a Cq value of < 40. Conclusion: A volume of 20 μl is the optimal volume, which is more efficient than the manufacturer's recommended volume of 25 ul.
Optimasi Volume Kit Da An Gene Untuk Deteksi SARS-CoV-2 dengan Real Time RT-PCR Seprianto Seprianto; Muhammad Arreza; Titta Novianti; Febriana Dwi Wahyuni; Oktaviani Naulita Turnip; Roaslein Putri; Henny Saraswati
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/628595

Abstract

Background: SARS-CoV-2 is a new type of coronavirus of the genus Betacoronavirus and the family Coronaviridae that causes a respiratory disease called COVID-19. The virus has a sheath and genetic material in the form of single-chain RNA. The genome structure of this virus is divided into two types, namely genes that encode non-structural proteins consisting of the ORF1a / ORF1b gene and genes that encode structural proteins consisting of spike glycoprotein (S), envelope (E), membrane glycoprotein (M), and nucleocapsid protein (N). Methods: The method of detecting SARS-CoV-2 with real time RT-PCR is the most recommended method because it has high specificity and accuracy. The specificity of a method is necessary to be able to specifically recognize the pathogen that causes the disease. Real time RT-PCR requires sampling with a swab on the oropharynx or nasopharynx to be examined in the laboratory which later the presence of viral RNA becomes a molecule that is assessed for diagnosis results. In this study, volume optimization was carried out on the Da An Gene kit used for the detection of SARS-CoV-2 with Reverse Transcription Polymerase Chain Reaction (Real time RT-PCR) with the aim of saving the use of reagents from available kits but with amplification results remaining optimal and accurate. Results: There were three SARS-CoV-2 RNA samples used consisting of N62, N63, and N79 samples and three types of total volume used were 20 μl, 15 μl, and 10 μl. The results of this study showed that the three positive samples contained SARS-CoV-2 with a Cq value of < 40. Conclusion: A volume of 20 μl is the optimal volume, which is more efficient than the manufacturer's recommended volume of 25 ul.
Co-Authors Abda Abda Adelia, Ismi Agustinus Robert Uria alfero Putra Iryanto Alham, Fiddini Anisa Febriyanti Ariyadi, Noprizal Aroem Naroeni Ayu, Yulia Agustina Azwar, Beni callista marsya Chikita, Daien Cyndi Prasetya Daflaini, Daflaini Darwata, Siti Riva DEWI PURNAMA Dewi Seswita Zilda Dewi Seswita Zilda Dewi, Lisa Rahma Dewi, Mustika Sandra Dina Hajja Ristianti, Dina Hajja Effendi, Desy Irafadillah Erani, Friska Fadila Fadila, Fadila Fajariyanto, Gunawan Febriana Dwi Wahyuni feby feby Feliatra Gloria, Meilina Gusvina, Fadilla Hana Nurullita Prestisia Harna, Harna Hasibuan, Molani Paulina Henny Saraswati Henny Saraswati Idi Warsah Irfandi, Ahmad Jofrishal Jofrishal Juniyati, Sintia Kevin Febrianus Moda Kibtiyah, Maryatul Lawrence, Yoel Lesmawan, Hendra Mahyuny, Siska Rita Manalu, Mely Grace Manalu Maulida, Maulida Mauliza, Mauliza Muhamad Amza Sidiq Muhammad Arreza nie nie Noer Khasanah Noperta, Noperta Nora, Adri Novianti, Titta Nugraha, Jaka Setia Nurmalasari, Mieke Nursanah, Nursanah Ogi Danika Pranata Oksal, Efriyana Oktaviani Naulita Turnip Oktaviani, Coryna PUSPITA LISDIYANTI Puspita Lisdiyanti PUTRI HANDAYANI Putri Handayani putri mega utami Putri, Mentari Darma Rahmah, Rasya Aulia Rahmayanti R, Andi Riansyah, Irza Rini Hidayati roaslein putri Roaslein Putri Roselein Putri Ruhmayanti, Nur Ayu Sa'pang, Mertien Sabar Pambudi Saraswati, Henny Saraswati, Henny Sari, Ratih Permana Seda, Ananda Putri Sianturi, Dody Jhonatan Wilfiez Sidauruk, Christina Siti Komariyah Situmorang, Larissa Kristina T.T. Soleha, Sovatunisa Suharsono Suharsono Surgasari, Tania Susanti, Sela Syamsul Rizal Titania Tjandrawati Nugroho Veza Azteria Widodo Widodo Wiriatmaja, Nuraini Ulya Yusro Nuri Fawzya Yusro Nuri Fawzya Zadrax, Adellino Zhafira, Alipya