Article Per Year (5 Year)
p-Index From 2021 - 2026
4.88
P-Index
This Author published in this journals
All Journal Bioscience Jurnal Pengajaran MIPA Biogenesis: Jurnal Ilmiah Biologi BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pendidikan IPA (JPPIPA) BIOEDUKASI Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology BIOEDUSCIENCE Jurnal Pengabdian Masyarakat AbdiMas Indonesian Journal of Biotechnology and Biodiversity Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Jurnal Kreativitas PKM Manuju : Malahayati Nursing Journal Jurnal Dedikasi Pendidikan Poltekita : Jurnal Ilmu Kesehatan Journal of Appropriate Technology for Community Services Jurnal Abdi Insani Jurnal Marketing Nusantara Science and Technology Proceedings Jurnal Pengabdian UNDIKMA Jurnal Pengabdian Nasional (JPN) Indonesia Jurnal Pengabdian kepada Masyarakat JURNAL STUDIA KOMUNIKA KATALIS: Jurnal Penelitian Kimia dan Pendidikan Kimia DIKSAINS : Jurnal Ilmiah Pendidikan Sains Jurnal Locus Penelitian dan Pengabdian Muhafadzah: Jurnal Ilmiah Bimbingan dan Konseling Islam RANGGUK: Jurnal Pengabdian Kepada Masyarakat
Seprianto, Seprianto
Program Studi Bioteknologi, Fakultas Ilmu-ilmu Kesehatan, Universitas Esa Unggul Jl. Arjuna Utara No. 09 Kebun Jeruk, Jakarta 11510
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

Published : 45 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Nusantara Science and Technology Proceedings

 1 
In Silico Analysis of Sox2 Gene for Pluripotency Detection at Mouse Embryonic Fibroblast and induced Pluripotent Stem Cell (iPSC) Aroem Naroeni; Seprianto; Kevin Febrianus Moda
Nusantara Science and Technology Proceedings 2nd Bioinformatics and Biodiversity Conference
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2101

Abstract

Stem cells are cells that have not been specialized and have a specific characteristic compared to other cells. There are several transcription factors like Sox2, Oct4, c-Myc, and Nanog to maintain embryonic stem cells. In pluripotent stem cells, sex-determining region Y-box 2 (Sox2) is a critical transcriptional regulator, and for somatic cell reprogramming, which involves returning differentiated cells to a pluripotent embryonic state by reversing their epigenetic arrangement. This study aimed to design primers for detecting the Sox2 gene expressed in Mouse Embryonic Fibroblasts and induced Pluripotency Stem Cell as pluripotency detection in stem cell research. In silico analysis was carried out to detect ofx2gene by using several software such as BLAST to search sequence Sox2 gene from Homo sapiens and Mus musculus, Bioedit for sequence alignment, SnapGene for PCR in silico, and PrimerBlast for online primer design. Primer candidates successfully designed were then analyzed for their secondary structure using NetPrimer. The results showed that forward primer (5'- CTACAGCATGTCCTACTCGCA -3') and reverse primer (5'- ACTTGACCACAGAGCCCA -3') were selected primers for M. musculus. Also, forward primer (5’-CTACAGCATGTCCTACTCGCA-3') and reverse primer (5'- ACTT-GACCACCGAACCCA-3') for Homo sapiens. Detection by PCR in silico using templates from H. sapiens and M. musculus sequences showed that the primers could specifically amplify the Sox2 gene in each species. Nevertheless, laboratory experiments need to be carried out for preliminary validation that has been designed. These primers will be used to measure the gene expression of Sox2 in qRT-PCR to detect the stemness characteristic of stem cells.
Co-Authors Abda Abda Adelia, Ismi Agustinus Robert Uria alfero Putra Iryanto Alham, Fiddini Anisa Febriyanti Ariyadi, Noprizal Aroem Naroeni Ayu, Yulia Agustina Azwar, Beni callista marsya Chikita, Daien Cyndi Prasetya Daflaini, Daflaini Darwata, Siti Riva DEWI PURNAMA Dewi Seswita Zilda Dewi Seswita Zilda Dewi, Lisa Rahma Dewi, Mustika Sandra Dina Hajja Ristianti, Dina Hajja Effendi, Desy Irafadillah Erani, Friska Fadila Fadila, Fadila Fajariyanto, Gunawan Febriana Dwi Wahyuni feby feby Feliatra Gloria, Meilina Gusvina, Fadilla Hana Nurullita Prestisia Harna, Harna Hasibuan, Molani Paulina Henny Saraswati Henny Saraswati Idi Warsah Irfandi, Ahmad Jofrishal Jofrishal Juniyati, Sintia Kevin Febrianus Moda Kibtiyah, Maryatul Lawrence, Yoel Lesmawan, Hendra Mahyuny, Siska Rita Manalu, Mely Grace Manalu Maulida, Maulida Mauliza, Mauliza Muhamad Amza Sidiq Muhammad Arreza nie nie Noer Khasanah Noperta, Noperta Nora, Adri Novianti, Titta Nugraha, Jaka Setia Nurmalasari, Mieke Nursanah, Nursanah Ogi Danika Pranata Oksal, Efriyana Oktaviani Naulita Turnip Oktaviani, Coryna PUSPITA LISDIYANTI Puspita Lisdiyanti PUTRI HANDAYANI Putri Handayani putri mega utami Putri, Mentari Darma Rahmah, Rasya Aulia Rahmayanti R, Andi Riansyah, Irza Rini Hidayati roaslein putri Roaslein Putri Roselein Putri Ruhmayanti, Nur Ayu Sa'pang, Mertien Sabar Pambudi Saraswati, Henny Saraswati, Henny Sari, Ratih Permana Seda, Ananda Putri Sianturi, Dody Jhonatan Wilfiez Sidauruk, Christina Siti Komariyah Situmorang, Larissa Kristina T.T. Soleha, Sovatunisa Suharsono Suharsono Surgasari, Tania Susanti, Sela Syamsul Rizal Titania Tjandrawati Nugroho Veza Azteria Widodo Widodo Wiriatmaja, Nuraini Ulya Yusro Nuri Fawzya Yusro Nuri Fawzya Zadrax, Adellino Zhafira, Alipya