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All Journal Bioscience Jurnal Pengajaran MIPA Biogenesis: Jurnal Ilmiah Biologi Jurnal Media Gizi Indonesia (MGI) BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pendidikan IPA (JPPIPA) BIOEDUKASI Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology BIOEDUSCIENCE Jurnal Pengabdian Masyarakat AbdiMas Indonesian Journal of Biotechnology and Biodiversity Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Jurnal Kreativitas PKM Manuju : Malahayati Nursing Journal Jurnal Dedikasi Pendidikan Poltekita : Jurnal Ilmu Kesehatan Journal of Appropriate Technology for Community Services Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Jurnal Abdi Insani Jurnal Marketing Nusantara Science and Technology Proceedings Jurnal Pengabdian UNDIKMA Jurnal Pengabdian Nasional (JPN) Indonesia Jurnal Pengabdian kepada Masyarakat JURNAL STUDIA KOMUNIKA KATALIS: Jurnal Penelitian Kimia dan Pendidikan Kimia DIKSAINS : Jurnal Ilmiah Pendidikan Sains Jurnal Locus Penelitian dan Pengabdian Jurnal Pengabdian Nasional (JPN) Indonesia Muhafadzah: Jurnal Ilmiah Bimbingan dan Konseling Islam RANGGUK: Jurnal Pengabdian Kepada Masyarakat
Seprianto, Seprianto
Universitas Esa Unggul
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Journal : BERITA BIOLOGI

 1 
TRANSFORMASI PLASMID REKOMBINAN pRI_101-AN MEMBAWA SISIPAN GEN cryIII MELALUI Agrobacterium tumefaciens Febriana Dwi Wahyuni; Muhamad Amza Sidiq; Seprianto Seprianto; Henny Saraswati
BERITA BIOLOGI Vol 21, No 1 (2022)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i3.4256

Abstract

Sweet potato is a plant that give many benefits because of its high carbohydrate content and is an alternative food source. Behind the benefits of sweet potatoes, there is a threat of pest attack that can cause a decrease in production of up to 19.91%. Sweet potato weevil attack on the leaves, stalks, stems, and tubers. The solution to against this pest attack is by engineering using the cry III gene mediated by A. tumefaciens. The aim of this study was to transform a recombinant plasmid carrying the insertion of the cry III gene into A. tumefaciens cells. The results showed that the recombinant plasmid pRI_101-AN carrying the insertion of the cry III gene was successfully transformed into A. tumefaciens cells by electroporation method by producing 36 recombinant clones on LB media with neomycin and kanamycin antibiotics. Results of colony PCR, DNA bands measuring 1900 bp were successfully amplified using gene-specific primers. Results of the isolation of plasmid DNA bands measuring 12000 bp were obtained from the results of electrophoresis. This size corresponds to the recombinant plasmid pRI_101-AN-cry III.
Co-Authors Abda Abda Adelia, Ismi Agustinus Robert Uria alfero Putra Iryanto Alham, Fiddini Alipya Zhafira Anisa Febriyanti Ariyadi, Noprizal Aroem Naroeni Aroem Naroeni Ayu, Yulia Agustina Azwar, Beni callista marsya Chikita, Daien Cyndi Prasetya Daflaini, Daflaini Darwata, Siti Riva DEWI PURNAMA Dewi Seswita Zilda Dewi Seswita Zilda Dewi, Mustika Sandra Dijaya, Rendy Dina Hajja Ristianti, Dina Hajja Dionysius Subali Effendi, Desy Irafadillah Efriyana oksal Erani, Friska Fadila Fadila, Fadila Fajariyanto, Gunawan Febriana Dwi Wahyuni feby feby Feliatra Gifari, Nazhif Gloria, Meilina Gusvina, Fadilla Hana Nurullita Prestisia Hardinsyah Harna, Harna Hasibuan, Molani Paulina Henny Saraswati Henny Saraswati Himarwan, Aditya Idi Warsah Irfandi, Ahmad Jofrishal Jofrishal Juniyati, Sintia Kevin Febrianus Moda Kibtiyah, Maryatul Lawrence, Yoel Lesmawan, Hendra Lisa Rahma Dewi Mahyuny, Siska Rita Manalu, Mely Grace Manalu Maulida, Maulida Mauliza, Mauliza Muhamad Amza Sidiq Muhammad Arreza Mury Kuswari nie nie Noer Khasanah Noperta, Noperta Nora, Adri Novianti, Titta Nugraha, Jaka Setia Nurmalasari, Mieke Nursanah Nursanah Ogi Danika Pranata Oktaviani Naulita Turnip Oktaviani, Coryna PUSPITA LISDIYANTI Puspita Lisdiyanti Putra, Felicia Kartawidjaja PUTRI HANDAYANI putri mega utami Putri, Mentari Darma Rahmayanti R, Andi Rasya Aulia Rahmah Rian Adi Pamungkas Riansyah, Irza Rimbawan , Rini Hidayati Rini Hidayati Roaslein Putri roaslein putri Roselein Putri Ruhmayanti, Nur Ayu Sa'pang, Mertien Sabar Pambudi Saraswati, Henny Saraswati, Henny Sari, Ratih Permana Seda, Ananda Putri Sianturi, Dody Jhonatan Wilfiez Sidauruk, Christina Siti Komariyah Situmorang, Larissa Kristina T.T. Soleha, Sovatunisa Suharsono Suharsono Susanti, Sela Syamsul Rizal Tania Surgasari Titania Tjandrawati Nugroho Veza Azteria Widodo Widodo Wiriatmaja, Nuraini Ulya Yusro Nuri Fawzya Yusro Nuri Fawzya Zadrax, Adellino