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Karakter Morfologi dan Analisis Daerah Conserved Gen Elongation Factor 1? (EF1?) pada Lepidotrigona terminata Suprianto Suprianto; Manap Trianto; Nur Alam; Ni Gusti Ayu Galuh Candra Kirana
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p05

Abstract

Lepidotrigona terminata is one of the stingless bees of the Apidae family. Morphological characteristics of L. terminata species are very rare due to their genetic characteristics. The elongation factor 1? gene has been studied in the growth and development of both morphology and molecular systematics. This study aims to describe the morphological character of L. terminata from Temanggung Regency, Central Java with a purposive sampling method and analyze the conserved gene elongation factor 1? area to explain genetic variation based on the proportion of DNA bases analyzed in silico and computational methods using a database in GenBank with number access DQ813123.1, DQ813125.1 and DQ813124.1. The results of the analysis explained that the morphological character of L. terminata is very difficult to distinguish from several other Lepidotrigona groups and it is suspected that the genetic trait of the elongation factor 1? gene has an influence on the morphological character of L. terminata. The proportion of DNA bases of the target gene has a length of about 682 bp to 780 bp with the highest proportion of bases found in Adenine (A) around 216 bp (31.67%) to 251 bp (32.18%), the least number of bases found in Guanin (G) around 121 bp (17.74%) to 140 bp (17.95%) and has twelve conserved gene regions. The data obtained from the research provide basic information to study the morphological characteristics and conserved regions of the elongation factor 1? gene in L. terminata. keywords: L. terminata, morphology, elongation factor 1?, in silico, computational methods
KEANEKARAGAMAN JENIS RAYAP PADA PERKEBUNAN KELAPA SAWIT DAN PERKEBUNAN KARET DI KABUPATEN BANJAR, KALIMANTAN SELATAN Manap Trianto; Fajri Marisa; Nur'aini Nur'aini; Sukmawati Sukmawati
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 5 No. 2 (2020)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v5i2.10716

Abstract

Rayap merupakan salah satu serangga yang berasal dari ordo Blatodea dan kelas Heksapoda. Kondisi habitat yang berbeda dapat mempengaruhi keanekaragaman rayap pada suatu habitat. Penelitian ini bertujuan untuk mempelajari keanekaragaman jenis rayap pada Perkebunan Kelapa Sawit dan Perkebunan Karet. Penelitian dilaksanakan pada bulan Mei 2020 di dua lokasi yaitu Perkebunan Kelapa Sawit (PKS) dan Perkebunan Karet (PK), Kabupaten Banjar, Provinsi Kalimantan Selatan. Hasil penelitian menunjukkan bahwa pada kedua lokasi penelitian terdapat sebanyak 2 famili, 9 genus, dan 11 spesies rayap. Keanekaragaman rayap pada Perkebunan Karet (H’ = 2.18) lebih tinggi dibanding pada lokasi Perkebunan Kelapa Sawit (H’ = 1,81).
JENIS-JENIS BIVALVIA DI PERAIRAN DANAU LINDU, KABUPATEN SIGI, PROVINSI SULAWESI TENGAH Manap Trianto; Syech Zainal; Daratul Maulydiia
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 6 No. 1 (2021): Bioma, Januari - Juni 2021
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v6i1.12558

Abstract

Bivalvia merupakan organisme yang memiliki ciri khas berupa dua bagian cangkang yang kurang lebih simetris. Bivalvia banyak dimanfaatkan oleh masyarakat sebagai sumber makanan dan mempunyai nilai gizi yang cukup tinggi. Penelitian ini bertujuan untuk mempelajari jenis-jenis bivalvia di Perairan Danau Lindu, Kabupaten Sigi, Provinsi Sulawesi Tengah. Penelitian dilaksanakan pada bulan April 2020 di tiga stasiun pengamatan. Berdasarkan hasil identifikasi, diperoleh 3 genus dari 5 spesies bivalvia yaitu Corbicula (C. javanica, C. moltkiana, C. fluminea), Pseudodon (P. vondembuschianus), dan Pilsbryoconcha (P. exilis).
Serangga Pengunjung Pada Spesies Bunga Anggrek Vanda tricolor Sukmawati Sukmawati; Manap Trianto; Nuraini Nuraini; Fajri Marisa; Moh. Dahri Kisman
Justek : Jurnal Sains dan Teknologi Vol 2, No 2 (2019): November
Publisher : Unversitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/justek.v2i2.3643

Abstract

Orchidaceae merupakan kelompok tumbuhan berbunga yang memiliki struktur unik, serta bentuk bunga yang sangat beragam, hal ini dapat mempengaruhi jenis serangga pengunjung. Penelitian ini bertujuan untuk mengetahui jenis serangga yang berkunjung pada bunga anggrek baik di dalam maupun diluar green house, khususnya spesies bunga anggrek Vanda tricolor. Penelitian ini dilaksanakan pada bulan November 2019. Pengamatan jenis serangga pengunjung pada jenis bunga anggrek dilakukan dengan metode scan sampling. Serangga pengunjung bunga Vanda tricolor di luar green house yaitu genus Crematogaster (anggota Ordo Hymenoptera: Familia Formicida), Anoplolepis (Hymenoptera: Formicidae), Xylocopa (Hymenoptera: Formicidae), Vespa (Hymenoptera: Formicidae), Drosophila (Diptera: Drosophilidae), Stomorhina (Diptera: Calliphoridae), Lucilia (Diptera: Calliphoridae), Chrysomya (Diptera: Calliphoridae), Musca (Diptera: Muscidae), Camptomyia (Diptera: Cecidomyiidae), Orchestes (Coleoptera: Curculionidae), Carpophilus (Coleoptera: Nititulidae), Stagmomantis (Mantodea: Mantiae) dan Ligocoris (Hemiptera: Miridae). Dari ke dua belas genus yang ditemukan mengunjungi bunga anggrek di luar green house, diketahui bahwa kedua genus yaitu Aphaenogaster dan Anoplolepis juga ditemukan mengunjungi bunga Vanda tricolor yang berada di dalam green house.
Eksplorasi Jenis-Jenis Ikan di Perairan Danau Lindu Provinsi Sulawesi Tengah Nurfadilah Rahma; Manap Trianto
Justek : Jurnal Sains dan Teknologi Vol 1, No 2 (2018): November
Publisher : Unversitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/justek.v1i2.3751

Abstract

Abstract: Fish are defined as vertebrates that live in water with characteristics of gills that function to take dissolve oxygen from water and fins used for swimming. This research aims to determine the species of fish that exist in Lindu Lake, Central Sulawesi Province. The method used is purposive sampling by using fishing gear net, fishing line and seser. Base on the result of this research was found 10 species of fish consisting of  9 Ordo and 6 Family, namely Oreochromis mossabicus, Oreochromis niloticus, Cyprinus caprio, Anabas testudineus, Osphroneus gourami, Anguilla marmorate, Aplocheilus panchax, Clarias batrachus, Channa striata and Puntius gonionatus. Abstrak: Ikan merupakan hewan bertulang belakang yang hidup di air dengan karakteristik memiliki insang yang berfungsi untuk mengambil oksigen terlarut dari air dan sirip yang digunakan untuk berenang. Penelitian ini bertujuan untuk mengetahui jenis ikan yang terdapat di Perairan Danau Lindu Provinsi Sulawesi Tengah. Metode yang digunakan adalah purposive sampling dengan menggunakan alat tangkap, pancing, dan seser. Berdasarkan hasil penelitian ditemukan 10 jenis ikan yang terdiri dari 9 ordo dan 6 family. Spesies ikan yang di peroeleh yaitu Oreochromis mossambicus, Oreochromis niloticus, Cyprinus carpio, Anabas testudineus, Osphronemus gourami, Anguilla marmorat, Aplocheilus panchax, Clarias batrachus, Channa striata dan        Puntius gonionatus.
Jenis Serangga Hama Pada Tanaman Cengkeh (Syzygium aromaticum) di Desa Salumpaga Kecamatan Tolitoli Utara Kabupaten Tolitoli Sukmawati Sukmawati; Manap Trianto; Nuraini Nuraini
Justek : Jurnal Sains dan Teknologi Vol 2, No 1 (2019): Mei
Publisher : Unversitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/justek.v2i1.3706

Abstract

Cloves (Syzygium aromaticum) are native to Indonesia which have high social and economic value, however cloves also have different types of pests. The latest information on insect pests in cloves is still very limited. This study aims to describe the types of insect pests found in clove (Syzygium aromaticum). This type of research is a descriptive exploratory research. Pest insect samples were taken at 3 observation locations, namely at a slope of 0%, a slope of <15% and a slope of> 15% and identification of the pests found. The results showed that at a 0% slope, the insect pests found were Coptotermes gestroi and Coptocercus biguttatus. At a slope of <15%, the insect pests found were Coptotermes gestroi, Hexamitodera semivelutina and Coptocercus biguttatus. Whereas at a slope of> 15%, the insect pests found were Coptotermes gestroi, Hexamitodera semivelutina, Coptocercus biguttatus and Nothopeus sp.
Keanekaragaman Genus Serangga Air sebagai Bioindikator Kualitas Perairan Manap Trianto; Nuraini Nuraini; Sukmawati Sukmawati; Moh Dahri Kisman
Justek : Jurnal Sains dan Teknologi Vol 3, No 2 (2020): November
Publisher : Unversitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/justek.v3i2.3562

Abstract

Abstract:  Aquatic insects are groups of insects that most of their life in the water body. Aquatic insects are part of macrozoobenthos. Aquatic insects are very important roles in the ecological system due to various reasons. Insects are bio-indicators of water in water bodies such as rivers. The aims of the study were to determine the diversity and the potential roles of aquatic insects in the Kali Batu Anyar ecosystem. The research method used purposive random sampling method. The sampling of the data was conducted by Surber nets. The samples obtained were identified and analyzed using the Shannon-Wiener index. The result consist of 6 Orders, 10 Families and 4 Genus were founded. According to the Shannon-Wiener diversity index of (H'), the diversity of aquatic insects in the upper Kali Batu Anyar was higher than the bottom part of the river with value of H’ was 3,6. By using SIGNAL 2 grade, Order Ephemeroptera, Trichoptera and Coleoptera were grouped to be very sensitive and a sensitive insect therefore those can be used as indicator for water quality. It can be concluded that Kali Batu Anyar was in good water conditions.Abstrak: Serangga air merupakan kelompok serangga yang sebagian hidupnya berada di badan air. Serangga air termasuk dalam makrozoobentos. Serangga air sangat penting dalam sistem ekologi karena berbagai alasan. Serangga air adalah bioindikator dalam badan air seperti sungai. Tujuan Penelitian ini untuk mengetahui keanekaragaman dan potensi peranan serangga air di ekosistem. Peneltian menggunakan metode Purposive Random Sampling. Pengambilan sampel menggunakan jaring Surber. Sampel yang diperoleh diidentifikasi dan dianalisis menggunakan indeks Shanon Wiener. Serangga air yang diperoleh di kelompokan pada 6 Ordo terbagi dari 10 Famili dan 4 Genus. Indeks keanekaragaman Shanon-Wiener H’ : 3,6 menunjukan keanekaragaman serangga air yang tinggi di hulu kali Batu Anyar tinggi. Berdasarkan SIGNAL 2 grade, serangga air anggota Ordo Ephemeroptera, Trichoptera dan Coleoptera yang ditemukan bersifat sangat sensitif dan sensitif dapat digunakan sebagai indikator kualitas air. Kali Batu Anyar dalam kondisi perairan yang baik.
Diversity, Abundance, and Distribution Patterns of Natural Enemy Insects on Chili (Capsicum annum L.) in Bincau Village, Banjar Regency, South Kalimantan Manap Trianto; Fajri Marisa; Moh Dahri Kisman
Biota Vol 13 No 2 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i2.304

Abstract

Chili is a widely cultivated plant by the community because of its profit. Currently, in maintaining the quality of chili, people commonly use a lot of pesticides. In contrast, naturally, there are biological agents in the shape of natural enemy insects, more effective, and environmentally friendly. This study aims to determine the diversity, abundance, and distribution patterns of natural enemy insects in chili plantation (Capsicum annum L.) at Bincau Village, Martapura District, Banjar Regency, South Kalimantan. The sample is collected by pitfall trap, yellow pan trap, and sweep net method. Obtained data are analyzed by Shannon Wienner index (H'), Meyer abundance index, Morisita index (Id), and PCA method for environmental parameter analysis. The results showed the diversity of natural enemy insects on chili plants was moderate (H' = 2.396). Furthermore, the highest abundance in research is demonstrated on Hymenoptera order, which consisted of 9 families, 16 species, and 1,009 individuals. The distribution pattern of natural enemy insects in chili plantations is clustered (Id > 1). Based on the PCA analysis method, the temperature has the most impact on natural enemy insects' presence in this study.
Tetragonula laeviceps (Hymenoptera: Apidae: Meliponini): Morphology, Morphometric, and Nest Structure Manap Trianto; Fajri Marisa; Moh Dahri Kisman
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (885.25 KB) | DOI: 10.22236/j.bes/424924

Abstract

Background: Stingless bees is one group of eusocial insects living together in the hive. There are around 46 species of stingless bee in Indonesia with different morphological, morphometric and hive structure characteristics. This study aimed to describe the morphological, morphometric and beehives structure of Tetragonula laeviceps from Yogyakarta. Methods: Survey method is used by taking three sampling points of bamboo, house building and livestock crates in Bantul Regency. Sampling points determined by Purposive sampling method. An XSZ-107 BN binocular microscope analyzed samples of T. laeviceps with Optilab viewer and Image Raster software. Results: The results showed morphological characters of T. laeviceps are dominated shiny-black body, brownish-yellow antennas, klipeus on a head covered by fine silver hair, brownish-yellow mandible with two teeth, mesonotum in thorax covered by brownish to black hair, scutellum extended to propodeum, the ribbon of hair on the dorsal thorax is not very clear, and the hind tibia is rather hairy. Morphometric of T. laeviceps included body length between 3.44 - 3.76 mm, head width 1.55 - 1.70 mm, front wing length with tegula 3.76 - 4.37 mm, length of rear limbs tibia 1.37-1.57 mm, and the number of hamuli as many as 5. The beehive structure consisted of oval-shaped entrance formed funnel and varying internal hive in terms of the number of saplings, pollen cells, and honey cells. Conclusions: T. laeviceps have morphological, morphometric and hive structure characteristics that are different from other species and variated compared to similar species from other regions.
Keanekaragaman serangga parasitoid (Ordo: Hymenoptera) pada areal persawahan dan kebun sayur Manap Trianto; Varsha Salsabillah
BIOEDUSCIENCE Vol 5 No 1 (2021): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.985 KB) | DOI: 10.22236/j.bes/515049

Abstract

Background: The Hymenoptera Order is one of the most dominant orders in the insect class, both in number of species and in their distribution in various habitats. The role of the Hymenoptera Order, especially parasitoids, is very important in a habitat. The purpose of this study was to determine the diversity of parasitoid insects, especially the order Hymenoptera in rice fields and vegetable gardens. Methods: This research is analytic descriptive. The research method used is a line transect using a sampling technique. The study was conducted in two research areas (rice fields and vegetable gardens) and used three fishing gears (malaise traps, buckets, and insect nets). Results: The results showed that the total number of insects caught was 5037 individuals from 10 orders and 80 family. The diversity of parasitoid insects in this study is in the rice fields H' = 1.80 (moderate diversity) and vegetable gardens H' = 2.26 (moderate diversity). Conclusions: Hymenoptera parasitoid insect diversity was highest in the vegetable garden area.
Co-Authors Abd Rauf Abdul Hakim Laenggeng Achmad Ramadhan Achmad Ramadhan Adnan, Bahana Aditya Agung Dwi Kurniawan Ahmad Ramadhan Akram, Akram Alimudin, Sukmawati Amalia Buntu Amiruddin Kasim Anisa Anisa Aprilya, Dinda Ari Pratama Artha, Fandy Ashari, Abdul Asmara, Dudun Candra Asriani Hasanuddin Astija Bahana Aditya Adnan Baharuddin Hamzah Biung, Yelsan Kanan Burhanuddin, Alga Patricia Bustamin Bustamin Bustamin Bustamin Carolus Noprianto Carolus Noprianto Daratul Maulydiia Delima, Nur Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham, Dirham EFENDI Efendi Efendi Efendi Fajri Marisa Fajri Marisa Fajri Marisa Fajri Marisa Faried, Dirham Fariza, Ifat Fatmah Dhafir Febriawan, Aan Hamsa, Hamsa Hari Purwanto Hari Purwanto Hartina, Siti Hayyatun Mawaddah Herjayanti, Nur Herlangga Adiputra Hidyanisa, Dhea Hj. Musdalifah Nurdin I Ketut Suada I Made Budiarsa I Nengah Kundera Idham Cholik Ramadhan Isninar, Isnainar Ivan Permana Putra Jayanti, Zulaikhah Dwi Kaini, Kaini Kaltisa, Kaltisa Kundera, I Negah Kusumaningati, Lilis Tri Laksono Trisnantoro Lembah, Rocky Reviko T. Lestari M.P Alibasyah M. Abimanyu Marisa, Fajri Masriani . Masrianih Moh Dahri Kisman Moh Dahri Kisman Moh Dahri Kisman Moh Dahri Kisman Moh. Dahri Kisman Mohammad Jamhari Mursito S. Bialangi Ni Gusti Ayu Galuh Candra Kirana Ni Putu Siswandari Ningsi, Ririn Apriani Noprianto, Carolus Novalina Serdiati Novi, Winda Cinthia NOVITASARI Nur Alam Nur Herjayanti Nur Herjayanti Nur Hikmah Nur'aini Nur'aini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Trianto Nurfadila A, Nurfadila A Nurfadilah Rahma Nuryanti, Sitti Pratama, Rizaldy Pratama, Sandi Fransisco Puspitasari, Amira Putri, Regina Triana RAFIQA RAFIQA, RAFIQA Rahayu, Irma Septi Raya Agni Ritman Ishak Paudi Rizaldy Pratama Rocky Reviko T. Lembah Rocky RT Lembah Rosichon Ubaidillah Saliyem, Saliyem Samsurizal, M. Sulaeman Saridewi, Uut Sitti Nur Fadhilah Sri Hardianti sukmawati Sukmawati Sukmawati Sukmawati Sukmawati Sukmawati Suprianto Sutrisnawati Mardin Syech Zainal Tiktiek Kurniawati Trianto, Nuraini Tuty Arisuryanti Varsha Salsabillah Wahyudi Wahyudi Warsih, Eko Winarsih Winarsih Windarsih, Yulia Yulia Windarsih Yulia Windarsih Yulia Windarsih Windarsih Zasmita, Eka