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All Journal Buletin Peternakan Biotropika Biology, Medicine, & Natural Product Chemistry Journal of Tropical Biodiversity and Biotechnology BIOMA : Jurnal Biologi Makassar Jurnal Biodjati Jurnal Penelitian Pendidikan IPA (JPPIPA) BIOEDUSCIENCE Jurnal Biologi Tropis Justek : Jurnal Sains Dan Teknologi Biosel: Biologi Science and Education Jurnal Pengabdian Magister Pendidikan IPA Biota BIO-EDU: Jurnal Pendidikan Biologi Jurnal Abdidas Koordinat : Jurnal Pembelajaran Matematika dan Sains Bioscientist : Jurnal Ilmiah Biologi Media Eksakta Journal Evaluation in Education (JEE) AGRI-TEK : Jurnal Penelitian Ilmu-ilmu Eksakta Interdisciplinary International Journal of Conservation and Culture Otus Education Jurnal Biologi dan Pendidikan Biologi Journal of Biology Science and Education
Manap Trianto
Universitas Tadulako
Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Pengaruh Ekstrak Tembuluk Kelapa Merah (Cocos nucifera var. rubescens) Terhadap Penurunan Kadar Glukosa Darah Pada Mencit (Mus musculus) Novi, Winda Cinthia; Sutrisnawati, Sutrisnawati; Ramadhan, Achmad; Trianto, Manap; Masrianih, Masrianih; Kundera, I Negah
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.16418

Abstract

This study aims to determine the effect of red coconut embryo extract (Cocos nucifera var. rubescens) on reducing blood glucose levels in mice (Mus musculus). The research employed a true experimental method using a completely randomized design (CRD). A total of 24 mice were used, divided into 6 groups with 5 replications each. The normal control group (KN) was given food and water ad libitum, while the positive control (K+), negative control (K−), and treatment groups (PI, PII, PIII) were induced with alloxan for 7 days. The positive control group (K+) was administered 1.3 mg of synthetic metformin per 20 grams of body weight. Both the normal control (KN) and negative control (K−) groups were given food and water ad libitum. The treatment groups PI, PII, and PIII were administered red coconut embryo extract at varying concentrations. Treatment group 1 (PI) received 40% extract, group 2 (PII) received 60%, and group 3 (PIII) received 80%. Observation data were analyzed using ANOVA. The results showed that the red coconut embryo extract had a significant effect on reducing blood glucose levels in alloxan-induced mice. The most effective concentration was 40% (PI), with an average blood glucose level of 98.6 mg/dL.
Pengaruh Ekstrak Bunga Rosella (Hibiscus sabdariffa L.) Terhadap Kadar Asam Urat Pada Tikus Putih (Rattus norvegicus) Yang Diinduksi LDL Fariza, Ifat; Ramadhan, Achmad; Sutrisnawati, Sutrisnawati; Mawaddah, Hayyatun; Rauf, Abd; Trianto, Manap
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.16594

Abstract

This study aims to determine the effect of Rosella flower extract (Hibiscus sabdariffa L.) on uric acid levels in White Rats (Rattus norvegicus) induced by LDL. The research method used is the Laboratory experiment method with a Completely Randomized Design (CRD) pattern. The number of samples used was 21 White Rats consisting of 7 treatment groups and 3 replications. The normal control group (KN) is a group that is only given food and drink, the negative control group (K-) is given LDL induction for 30 days, the positive control group (K+) is given LDL induction for 30 days and allopurinol, and the group given LDL induction for 30 days and Rosella flower extract, namely concentrations of 25% (PI), 50% (PII), 75% (PIII), and 100% (PIV) for 14 days. The data obtained were tabulated and analyzed using one-way ANOVA statistical test using SPSS version-30. The results showed that Rosella flower extract had a significant effect (p <0.05) on reducing uric acid levels. The effective concentration for reducing uric acid levels is a concentration of 50% (PII) compared to concentrations of 75% and 100% because there are chemical compounds that are antagonistic to the active compound, thus reducing the extract's ability to reduce blood uric acid levels. The results of the Rosella flower study can reduce uric acid levels so that it can be used as an alternative natural treatment.
Identifikasi Bacillus sp. pada Fermentasi Tapai Singkong Saridewi, Uut; Kundera, I Nengah; Budiarsa, I Made; Nurdin, Musdalifah; Trianto, Manap; Windarsih, Yulia
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.16707

Abstract

This study aims to identify Bacillus sp. in cassava tapai fermentation that has potential as a probiotic ingredient. The type of research used is descriptive exploratory using the Standard Plate Count (SPC) method, Gram staining, and biochemical testing with the Microbant System 12B. Cassava tapai samples were obtained by fermenting them for 5 days. Fermentation of cassava tapai was carried out in the Biology Education Laboratory. The results of the study showed that (1) The Standard Plate Count test yielded the highest value on the 4th day of observation at 231x10-2 CFU/ml, while the lowest value was observed on the 1st day at 162x10-2 CFU/ml. (2) Gram staining of the bacterial isolates revealed Gram-positive rod-shaped morphology. (3) Biochemical testing using the Microbant System 12B showed the ability to ferment several types of carbohydrates such as sorbitol, sucrose, lactose, arabinose, adonitol, and salicin, but it cannot ferment inositol, malonate, rhamnose, raffinose, and arginine. Thus, it can be concluded that the bacteria identified from cassava tapai fermentation can ferment in accordance with the physiological capabilities of Bacillus sp.
Kajian situasi perlebahan, tingkat pengetahuan peternak lebah tentang lebah madu lokal, karakteristik produksi dan perilakunya di Desa Namo Kecamatan Kulawi Kabupaten Sigi Sabran, Moh; Trianto, Manap; Suleman, Samsurizal M.; Kasim, Amiruddin
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 4 (2023): Oktober-Desember 2023
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v6i4.3700

Abstract

Budidaya lebah madu merupakan kegiatan yang ramah lingkungan untuk menunjang kebutuhan hidup bahkan mendukung konservasi dan pelestarian keanekaragaman hayati. Usaha tersebut dapat dilakukan oleh kaum perempuan, dengan tekonologi tepat guna yang mudah diakses oleh masyarakat baik yang berkemampuan ekonomi rendah maupun memiliki pengetahuan dan ketrampilan yang rendah. Usaha budidaya lebah madu di Desa Namo berpotensi sebagai obyek wisata edukatif di Sigi. Demikian pula, produk dari lebah madu, dapat ditonjolkan sebagai produk oleh-oleh yang khas. Pengusul dan Tim dari Program Studi Pendidikan Biologi FKIP UNTAD telah berinteraksi dengan Kelompok Tani Lebah Madu dengan ketua Bapak Anwar Pelobo. Bersama tim, pengusul telah melakukan survey yang ditindaklanjuti dengan pelatihan singkat mengenai budidaya lebah madu. Pengusul secara berkala juga melakukan kunjungan untuk berdiskusi dengan para peternak lebah madu. Melalui kegiatan pengabdian kepada masyarakat diharapkan terwujudnya Kampung Madu sebagai desa wisata edukasi dan pusat pelatihan dengan konsep Education for Sustainable yang meliputi keseimbangan ekonomi (produk madu asli), pelestarian potensi lokal (hutan rakyat, pekarangan dan lebah madu lokal) dan sosial (pemberdayaan masyarakat dengan melibatkan Kelompok Tani utamanya pada kelompok pemuda dan perempuan). Kegiatan ini telah terlaksana sesuai dengan usulan dan berhasil meningkatkan kapasitas kelembagaan utamanya dengan meningkatkan jumlah anggota kelompok tani serta meningkatkan pengetahuan dan keterampilan budi daya lebah madu para anggota. Kegiatan pelatihan secara tidak langsung juga membuka peluang untuk warga masyarakat menyampaikan penilaian mereka terhadap jalannya kelompok dan meningkatkan rasa memiliki di antara warga. Kegiatan pengabdian juga mencoba mengenalkan penggunaan beberapa peralatan standar budi daya. Diharapkan alat-alat tersebut akan mendorong efisiensi dan keamanan budi daya lebah madu di Desa Namo. Untuk terwujudnya Desa Namo sebagai pusat wisata edukatif dan sumber madu, sebaiknya dibentuk jaringan kerja antara masyarakat (peternak lebah), LSM, dan CSR BUMN yang diharapkan dapat bersinergi mengembangkan perlebahan dan wisata edukatif di Desa Namo, Kecamatan Kulawi, Kabupaten Sigi, sesuai harapan para pemegang kepentingan di Desa Namo.
The Impact of Problem Based Learning with Mind Mapping on Learning Outcomes in Ecosystem Lesson Mohammad Jamhari; Yulia Windarsih; Manap Trianto
Jurnal Penelitian Pendidikan IPA Vol 11 No 7 (2025): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i7.11284

Abstract

Education plays a critical role in shaping the cognitive, affective, and psychomotor development of students. In the 21st century, students are expected not only to acquire knowledge but also to develop the ability to think critically, solve problems, and apply concepts in real-life situations. For this reason, this study aims to determine the effect of applying the problem-based learning model assisted by mind mapping on student learning outcomes in ecosystem lesson. This research employed a quasi-experimental approach using the Pretest-Posttest Non-Equivalent Control Group Design, involving both control and experimental classes. The study population included all tenth-grade students at SMA Negeri 1 Biau. Through purposive sampling, four classes were selected, totaling 121 students. Learning outcomes data were collected using a test instrument comprising 30 multiple-choice questions. The data were analyzed using the Mann-Whitney statistical test at a significance level of 0.05. The analysis results revealed that the use of the problem-based learning model combined with mind mapping had a statistically significant impact on student learning outcomes, with a p-value less than 0.05 (0.000 < 0.05). Therefore, it can be concluded that the integration of mind mapping within the problem-based learning model has a positive influence on learning achievement. These findings suggest that this instructional approach is effective and can be implemented in lessons focused on ecosystem.
The Effect of Seaweed Soy Tempe Extract on the Reduction of Blood Sugar Levels in Alloxan Induced Rats (Mus musculus) Abdul Hakim Laenggeng; Asriani Hasanuddin; Sitti Nuryanti; Manap Trianto
Jurnal Penelitian Pendidikan IPA Vol 11 No 7 (2025): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i7.11307

Abstract

Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia due to impaired insulin secretion or action. This study aimed to examine the antihyperglycemic effect of seaweed soybean tempeh extract in alloxan-induced diabetic mice. A Completely Randomized Design (CRD) was used with 20 healthy male mice (2–3 months old, 25–30 g), divided into five groups: negative control, positive control (metformin), and three treatment groups receiving extract doses of 25, 75, and 125 mg/kg BW. Prior to treatment, mice underwent acclimatization to ensure uniform baseline conditions. Blood glucose levels were measured to assess the effects of the extract. Data were analyzed using ANOVA followed by the Least Significant Difference (LSD) test. The results showed that alloxan effectively induced hyperglycemia by damaging pancreatic beta cells. Administration of seaweed soybean tempeh extract significantly reduced blood glucose levels (p < 0.05). The highest dose (125 mg/kg BW) produced the most notable effect, comparable to metformin, while the 75 mg/kg BW dose also demonstrated significant efficacy. Both were significantly more effective than the lowest dose. In conclusion, seaweed soybean tempeh extract has potential as an alternative antihyperglycemic agent, especially at higher doses.
Diversity of Insect Visitors to Oil Palm Flowers in Smallholder Plantations as a Potential Resource for Environment Based Learning in Education Samsurizal M Suleman; Manap Trianto; Syech Zainal
Journal Evaluation in Education (JEE) Vol 6 No 3 (2025): July
Publisher : Cahaya Ilmu Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37251/jee.v6i3.1737

Abstract

Purpose of the study: Insect visitors to oil palm flowers, an important component in the pollination process and ecosystem services, are evaluated for their diversity in this study. The research explores their potential as a resource for environment-based learning rooted in local contexts within smallholder plantations, aiming to raise awareness and support sustainable education practices in the community. Methodology: Insects were collected using sweep nets, yellow pan traps, and direct observation. Identification was conducted using a Nikon SMZ445 stereo microscope and reference to entomological keys. Data analysis was carried out descriptively, focusing on species composition and abundance. An e-pocket book was developed as a learning resource. Main Findings: The results showed a total of 50 morphospecies of insect visitors on both male and female oil palm flowers. The number of insect species visiting male flowers was higher, consisting of 38 morphospecies belonging to 21 families and 10 orders. The results of the study were developed into a local wisdom-based pocket book focused on the diversity of insect visitors to oil palm flowers in smallholder plantations. The learning resource was validated by content experts, design experts, media experts, and students, with an average score of 81.7%, indicating it is highly suitable for use in environment-based education. Novelty/Originality of this study: This study highlights insect diversity in smallholder oil palm plantations, which is rarely documented. It also links ecological data with educational applications, providing a model for utilizing local biodiversity as a teaching resource in environmental and biology education.
Diversity and Composition of Insect Species Associated with Natural Rattan Habitats in Namo Village, Kulawi, Central Sulawesi Musdalifah Nurdin; Samsurizal M Suleman; Syech Zainal; Moh Sabran; Raya Agni; Manap Trianto
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1289-1295

Abstract

Tropical forest ecosystems are home to diverse insect communities that play essential ecological roles, including pollination, decomposition, and nutrient cycling. Among the key forest plants, rattan (Arecaceae) provides both ecological and economic benefits and serves as an important microhabitat for various insect species. This study aimed to identify and analyze the diversity of insect species associated with natural rattan habitats in Namo Village, Kulawi District, Central Sulawesi, Indonesia. Insect sampling was carried out using sweep nets, pitfall traps, and yellow pan traps along rattan-dominated forest transects. A total of 125 individual insects were collected, representing 7 orders, 28 families, and 37 species. The order Hymenoptera showed the highest abundance (41.6%), dominated by Formicidae and Apidae families, followed by Coleoptera (25.6%) and Diptera (17.6%). The Shannon–Wiener Diversity Index (H’ = 2.84) indicated moderate species diversity with high evenness (E = 0.83). Functionally, the insect community consisted of pollinators (38.2%), herbivores (25.4%), decomposers (19.6%), and predators (16.8%). These findings demonstrate that natural rattan ecosystems support diverse and functionally balanced insect assemblages, reflecting stable ecological conditions and minimal anthropogenic disturbance. Conserving natural rattan stands is therefore crucial for maintaining biodiversity and sustaining ecological functions such as pollination and decomposition within tropical forest ecosystems.
Analysis of Nutritional Composition and Antioxidant Activity of Yogurt Enriched with Butterfly Pea (Clitoria ternatea L.) Extract Yulia Windarsih; I Made Budiarsa; Manap Trianto; Akram Akram; Abdul Ashari
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.831-841

Abstract

The growing demand for functional foods has encouraged the development of dairy products enriched with natural bioactive ingredients to improve nutritional value and health benefits. This study aimed to analyze the nutritional composition and antioxidant activity of yogurt enriched with butterfly pea (Clitoria ternatea L.) extract. Yogurt was prepared from fresh goat milk with varying concentrations of butterfly pea extract and evaluated for protein, fat, carbohydrate, moisture, ash, vitamin C contents, antioxidant activity, and sensory acceptance. Proximate analysis, UV–Vis spectrophotometry, and the DPPH method were used for chemical determinations, while a hedonic test assessed color, taste, aroma, and texture. Results showed that increasing butterfly pea extract significantly decreased protein and fat contents but increased vitamin C and antioxidant activity, with the highest values observed in yogurt containing 60% extract and 4% formulation (N60K2). Moisture and ash contents were not significantly affected. Sensory evaluation indicated that yogurt with 60% extract and 2% formulation (N60K1) was preferred regarding color, taste, aroma, and texture. Considering compliance with the Indonesian National Standard (SNI) and consumer acceptance, N60K1 was the optimal formulation. The findings suggest that butterfly pea extract can enhance the functional properties of yogurt, particularly its antioxidant potential, while maintaining acceptable sensory quality. This provides insights for developing functional dairy products with natural plant-based bioactives.
Molecular Identification of Lepidopteran Predators of Coccidae on Coffee Plants (Coffea canephora) in Sigi Regency I Made Budiarsa; Manap Trianto; Bahana Aditya Adnan
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.983-992

Abstract

This study aimed to identify the species of Lepidopteran predators that prey on soft scale insects (Coccidae) found on coffee plants in Sigi Regency using molecular techniques. DNA was extracted using the modified CTAB method (Doyle & Doyle, 1990). PCR amplification targeted the COI gene using universal primers LCO1490 and HCO2198 with a GeneAmp PCR System 9700. Visualization was done using 1% agarose gel and UV-transilluminator. Sequencing was conducted externally. Data were analyzed using BioEdit 7.2.5, BLAST, BOLD Systems, and MEGA11 software. The DNA sequence of the predator sample showed 97.91% similarity to Autoba rubra based on GenBank and 97.59% in BOLD Systems. Phylogenetic analysis confirmed a close genetic relationship with A. rubra, distinct from Eublemma. Morphological similarities with Eublemma were misleading, highlighting the accuracy of molecular identification. This study is the first to confirm the identity of a Lepidopteran predator of coffee scale insects in Indonesia using molecular techniques, revealing its close relation to Autoba rubra. The results correct previous morphological misidentifications and contribute new data on predator diversity in biological control contexts.
Co-Authors Abd Hakim Laenggeng Abd Rauf Abd Rauf Abd. Rauf Abdul Ashari Abdul Hakim Laenggeng Achmad Ramadhan Achmad Ramadhan Achmad Ramadhan Adi Fahri Agung Dwi Kurniawan Ahmad Ramadhan Akram Akram Alimudin, Sukmawati Amalia Buntu Amelia A Riuh Amiruddin Kasim Andi Nurjannah Anisa Anisa Aprilya, Dinda Ari Pratama Artha, Fandy Ashari, Abdul Asmara, Dudun Candra Asriani Hasanuddin Astija Aulia Putri Ramadhani Bahana Aditya Adnan Bahana Aditya Adnan Baharuddin Hamzah Biung, Yelsan Kanan Burhanuddin, Alga Patricia Bustamin Bustamin Bustamin Bustamin Bustamin Bustamin Bustamin Carolus Noprianto Carolus Noprianto Daratul Maulydiia Delia Nurhaliza Delima, Nur Devi Devi Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham, Dirham EFENDI Efendi Efendi Efendi Fajri Marisa Fajri Marisa Fajri Marisa Fajri Marisa Faried, Dirham Fariza, Ifat Fatma Dhafir Fatmah Dhafir Febriawan, Aan Hamsa, Hamsa Hari Purwanto Hari Purwanto Hartina, Siti Hayyatun Mawaddah Henny Chandra Herjayanti, Nur Herlangga Adiputra Hidyanisa, Dhea Hikma Abia Hj. Musdalifah Nurdin I Ketut Suada I Made Budiarsa I Made Budiarsa I Nengah Kundera Idham Cholik Ramadhan Iin B Mantulangi Isnainar Isnainar Isninar, Isnainar Ivan Permana Putra Jihan Winarti Kaini, Kaini Kaltisa, Kaltisa Khaerunnisa Kusumaningati, Lilis Tri Laksono Trisnantoro Lembah, Rocky Reviko T. Lestari M.P Alibasyah Lestari M.P Alibasyah Lestari MP Alibasyah Lilies Tangge M. Abimanyu Marisa, Fajri Masriani . Masrianih Masrianih Masrianih Mia Pinasti Azizah Moh Dahri Kisman Moh Dahri Kisman Moh Dahri Kisman Moh Dahri Kisman Moh Syahrial Farawansyah Moh. Dahri Kisman Mohammad Jamhari Murni Ammalia Mursito S Bialangi Mursito S. Bialangi Mursito S. Bialangi Musdalifah Nurdin Muslika Nifatun Umaya Ni Gusti Ayu Galuh Candra Kirana Ni Kadek Deladarmi Yanti Ni Putu Siswandari Ningsi, Ririn Apriani Noprianto, Carolus Novalina Serdiati Novi, Winda Cinthia NOVITASARI Nur Alam Nur Herjayanti Nur Herjayanti Nur Hikmah Nur'aini Nur'aini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Trianto Nurfadila A, Nurfadila A Nurfadilah Rahma Nurly Maftukhah Nurul Amna Moh Said Ongky Ongky Pratama, Rizaldy Pratama, Sandi Fransisco Puspitasari, Amira Putri, Regina Triana Rafiqa Rafiqa RAFIQA RAFIQA, RAFIQA Rafiqah Rafiqah Rahayu, Irma Septi Raya Agni Rifana Lemba Risnawati Risnawati Ritman Ishak Paudi Rizaldy Pratama Rizkia Khairunnisa Rocky Reviko T. Lembah Rocky RT Lembah Rosichon Ubaidillah Sabran , Moh. Safani Safani Salbia Salbia Saliyem, Saliyem Samsurizal, M. Sulaeman Saridewi, Uut Sinta Sinta Sitti Nur Fadhilah Sitti Nuryanti Sri Hardianti sukmawati Sukmawati Sukmawati Sukmawati Sukmawati Sukmawati Sukmawati Sukmawati Suprianto Sutrisnawati Mardin Sutrisnawati Sutrisnawati Sutrisnawati Sutrisnawati Syech Zainal Syech Zainal Tiara Tiara Tiktiek Kurniawati Tiktiek Kurniawati Trianto, Nuraini Tuty Arisuryanti Uzaini Nur Rahma Vadillah Vadillah Varsha Salsabillah Vita Indri Febriani Wahyudi Wahyudi Warsih, Eko Wayan Windyawati Winarsih Winarsih Windarsih, Yulia Yunita Messe Zasmita, Eka Zulaikhah Dwi Jayanti Zulaikhah Dwi Jayanti