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UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN PORANG (Amorphophallus oncophyllus Prain.) TERHADAP BAKTERI Escherichia coli DAN BAKTERI Salmonella typhi Marpaung, Jon Kenedy; Suharyanisa; Darwita Juniwati Barus; Putri
JURNAL TEKNOLOGI KESEHATAN DAN ILMU SOSIAL (TEKESNOS) Vol. 3 No. 2 (2021): JURNAL TEKNOLOGI, KESEHATAN DAN ILMU SOSIAL (TEKESNOS)
Publisher : Universitas Sari Mutiara Indonesia

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Abstract

Escherichia coli and Salmonella typhi are included in the pathogenic bacteria. These bacteria can cause infectious diarrheal diseases. Porang (Amorphophallus oncophyllus Prain.) leaves contain antibacterial compounds that are effective against bacterial growth. This study aims to determine the antibacterial activity of Porang leaf extract against Escherichia coli and Salmonella typhi bacteria and to determine the difference in antibacterial activity with the use of various concentrations of extracts on bacterial growth. Porang leaf extract was obtained by maceration method using 96% solvent. This test used the paper disc agar diffusion method with concentrations of 10%, 15%, 20%, and 25% against Escherichia coli and Salmonella typhi bacteria, positive control used chloramphenicol, and negative control DMSO, then tested using statistics using the ANOVA method. The results of the study on Escherichia coli bacteria obtained that the concentration and diameter of the inhibition zone with an average of three repetitions were 10% (9.41mm), 15% (11.4 mm), 20% (12.4 mm), 25% (13 .3 mm) and for Salmonella typhi, 10% (12.8 mm), 15% (13.6 mm), 20% (15.5 mm), and 20% (17.5 mm). In the positive control of chloramphenicol, the diameter of the inhibition zone was obtained with an average of three repetitions for each bacterium, namely Escherichia coli (27.5 mm) and Salmonella typhi (29.4 mm) while the DMSO negative control did not show any inhibition. The conclusion of this study showed that Porang leaf extract had activity against Escherichia coli and Salmonella typhi bacteria with strong criteria, seen from the increase in the concentration of the test solution had an increase in the diameter of the growth inhibition zone on bacteria,
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN PISANG EMAS (Musa acuminata Colla.) TERHADAP BAKTERI Staphylococcus aureus Suharyanisa; Binsar Sitorus; Yenni Gustiani tarigan; Hestina; Suedy Jordan Nainggolan
JURNAL TEKNOLOGI KESEHATAN DAN ILMU SOSIAL (TEKESNOS) Vol. 3 No. 2 (2021): JURNAL TEKNOLOGI, KESEHATAN DAN ILMU SOSIAL (TEKESNOS)
Publisher : Universitas Sari Mutiara Indonesia

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Abstract

Banana gold (Musa acuminata Colla.) is a type of banana that contains a lot of sugar so that it has a sweeter taste when compared to other types of bananas. So a study was conducted on the test of antibacterial activity of Ethanol extract golden banana leaves (Musa acuminata Colla.) against Staphylococcus aureus bacteria. This study aims to determine the antibacterial activity of ethanol extract of golden banana leaves and find out at what concentrations have the most influence on the inhibitory power of staphylococcus aureus bacteria growth. This study was conducted by extracting Banana Leaves using 96% ethanol by the maceration method, then the extract obtained was made with several concentrations, namely 20, 40, and 80mg/mL. then antibacterial activity testing is carried out by the method of diffusion agar. In ethanol extract against Staphylococcus aureus bacteria with a concentration of 80mg/mL (14.62 ± 0.99) at a concentration of 40mg/mL (14.23 ± 0.70) and at a concentration of 20mg/mL (12.68 ± 0.42). Ethanol extract concentration of 80 mg/mL has a good ability to inhibit the growth of staphylococcus aureus bacteria with an inhibitory zone (14.62 ± 0.99) which is categorized as strong.
UJI DAYA HAMBAT INFUSA BATANG BROTOWALI (Tinospora crispa (L)) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli Suharyanisa; Evarina Sembiring; Ester Saripati Harianja; Sahma Desriani Sinaga
JURNAL TEKNOLOGI KESEHATAN DAN ILMU SOSIAL (TEKESNOS) Vol. 3 No. 2 (2021): JURNAL TEKNOLOGI, KESEHATAN DAN ILMU SOSIAL (TEKESNOS)
Publisher : Universitas Sari Mutiara Indonesia

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Abstract

Background: Brotowali stems (Tinospora crispa (L)) contain many chemical compounds that are efficacious in curing various diseases. Brotowali stems (Tinospora crispa (L)) contain bitter compounds (Picroretin), alkaloids, tinocrisposide, saponins, columbine, palmatin, kaemferol, and starch. Escherichia coli (Gram negatitve) is a bacterium that causes diarrhea. Research objectives: The purpose of this study was to determine the inhibitory power of brotowali stem infusion on the growth of Escherichia coli bacteria and to determine the effective concentration of brotowali stem infusion on the growth of Escherichia coli. Research method: This research methode uses an experimental methode of agar diffusion method of agar diffusion method to test the antibacterial and infusion of brotowali stems with concentrations of 25%w/v. 50%w/v, and 75%w/v. Results: The results of this study showed that concentrations of 25%, 50%, 75% of brotowali stem infusion had antibacterial inhibition against the growth of Escherichia coli as indicated by the inhibitory diameters of 7,5mm, 7,93mm, 10mm, respectively. Conclusion:  The conclusion of this study can be concluded that the infusion of brotowali stems (Tinospora crispa (L)) can inhibit the growth of Escherichia coli bacteria.