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All Journal Jurnal Veteriner Media Kedokteran Hewan Airlangga Development Journal Ovozoa: Journal of Animal Reproduction Indian Journal of Forensic Medicine & Toxicology Jurnal Kedokteran Hewan

Sri Pantja Madyawati

Unknown Affiliation

Author-ID : 2019316

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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PROFIL PROTEIN TIROSIN KINASE DALAM SEMINAL PLASMA DOMBA MERINO DENGAN TEKNIK SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS Tantri Nawangwulan; Sri Pantja Madyawati; Hani Plumeriastuti; Trilas Sardjito; Suherni Susilowati; Erma Safitri
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The aim of this study was to find out protein tyrosine kinase profile in Merino Sheep seminal plasma. This study consist of collecting semen of Merino Sheep containing plasma and spermatozoa, the separation between seminal plasma and spermatozoa, then tyrosine kinase analysis using Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE). Semen was collected by artificial vagina and then centrifugated for 40 minutes at 4000 rpm to separated seminal plasma and spermatozoa. Protein was analyzed using SDS-PAGE, to separate each protein based on their molecular weight. The result showed that there were 13 protein bands in 3 seminal plasma samples with an average 149,63 kDa, 139,7 kDa, 114,97 kDa, 109,3 kDa, 97,33 kDa, 93,83 kDa, 86,23 kDa, 77,6 kDa, 64,6 kDa, 52,3 kDa, 41,93 kDa, 38,13 kDa, and 34,5 kDa. In conclusion, it is believed that tyrosine kinase located on the sixth band with molecular weight 93,83 kDa.

PENGARUH PEMBERIAN EKSTRAK KULIT BAGIAN DALAM SEMANGKA (Citrullus lanatus) TERHADAP KEUTUHAN MEMBRAN PLASMA DAN ABNORMALITAS MORFOLOGI TIKUS JANTAN (Rattus norvegicus) SETELAH DIPAPAR SUHU PANAS Dinda Reisinta; Widya Paramita L; Tatik Hernawati; Dewa Ketut Meles; Sri Pantja Madyawati; Tri Wahyu Suprayogi
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This study aims to determined the effect of watermelon’s inner rind extract (Citrullus lanatus) towards the integrity of the plasma membrane and morphological abnormalities of male Norway rats’s spermatozoa (Rattus norvegicus) after being exposed to hot temperature. The content of lycopene in the inner rind of watermelon serves an antioxidant counteracting free radicals. A total of 20 male Norway rats (Rattus norvegicus) were used in this study with an average age of 2 months. The study was divided into five treatments and four replications, consisting of: K(-) as negative control given CMC Na 0.5% as much as 0.5 ml, P0 as positive control was exposed to 40° C for 1 hour and CMC Na 0.5% of 0.5 ml. P1, P2 and P3 are exposed to 40° C for 1 hour and inner rind extracts of watermelon with a multilevel dose of P1: 20mg/head/day, P2: 40mg/head/day, and P3: 80mg/head/day that each dose was dissolved in CMC Na 0.5% of 0.5 ml. The results showed that giving inner rind extract of watermelon to the rats increased the integrity of the plasma membrane and decreased morphological abnormalities of male rat’s spermatozoa (Rattus norvegicus). The result of increased the integrity of the plasma membrane and highest decreased morphological abnormality was shown by P3 group given a dose of 80 mg/head/day.

PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI Mayrena Ayu Cesaria; A.T.Soelih Estoepangestie; Suherni Susilowati; Tatik Hernawati; Sri Pantja Madyawati; Indah Norma Triana
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This study aimed to determine the influence of mixture diluter of chicken egg yolk and young coconut water on the integrity of plasma membrane and abnormalities of Sapudi sheep spermatozoa. The experimental design used in this study was a complete random method. The data was analysed using ANOVA, and if there is a significant difference then proceed with Duncan’s Multiple Range Test ( DMRT ) at significance level of 5%. Based on the result of the research, the influence of mixture diluter of chicken egg yolk and young coconut water was not significantly different ( p > 0,05 ) on the intact plasma membrane of spermatozoa on the first day until the fourth day. Interaction between treatment and storage duration had no effect on spermatozoa abnormality on the first day, second day and the fourth day. It can be concluded that mixture diluter of chicken egg yolk and young coconut water had a good effect on the plasma membrane and spermatozoa abnormalities up to the third day.

DAMPAK PEMBERIAN ROTI AFKIR TERHADAP EFISIENSI REPRODUKSI SAPI PERAH Friesian Holstein DI WILAYAH KERJA KPSP SETIA KAWAN NONGKOJAJAR, PASURUAN Ricko Nurdianto S.; Sri Hidanah; Sri Pantja Madyawati; Abdul Samik; wurlina wurlina; Tri Wahyu Suprayogi
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this research is to know the impact of bread waste to efficiency reproduction of Friesian Holstein cow in regional working KPSP Setia Kawan Nongkojajar, Pasuruan by value Service per Conception (S/C), Conception Rate (CR), Days Open (DO). Method in this research is the dependent variable observed is the reproductive efficiency, the dependent variable as a supporter is the cow has given birth, the healthy dairy cow, the feed volume given. The data obtained is processed by using descriptive analysis to get the reproduction efficiency figure. The result of the research indicate that the value of Service per Conception (S/C) = 1.5 ± 0.69 times, Days Open (DO) = 95.6 ± 11.32 days and Conception Rate (CR) = 65% and Fertility Status (FS) = 72,4%. Results of this study can be concluded that bread waste provided by the breeder as an additional feed for dairy cow has no significant effect.

Penggunaan kombinasi Gonadotropin untuk pengobatan hipofungsi ovarium pada sapi perah Novia Alfi Masruro; Sri Mulyati; Sri Pantja Madyawati; Abdul Samik; Hermin Ratnani; Nenny Harijani
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This case report aims to determine the response of estrus and pregnancy rates in dairy cows with ovarian hypofunction by administering a combination of gonadotropin hormones. The sample used was ten dairy cows that had been calving, were not pregnant, showed no signs of estrus for more than 60 days after the last calving and had ovarian hypofunction based on rectal palpation. All dairy cows were injected 300 IU PG-600, then divided into two groups, each injected with 100 and 300 IU hCG respectively for first group and second group, intramuscularly at the time of insemination. The results showed that the estrus response occurred in all cows, in 6-8 (6.8 ± 0.84) days (first group), and 6-7 (6.2 ± 0.45) days (second group). Trans-rectal palpation pregnancy diagnosis 60 days after artificial insemination showed that all dairy cows in both groups were pregnant. It could be concluded that the treatment of dairy cows suffering from ovarian hypofunction with a combination of 300 IU PG-600 and 100 IU hCG was sufficient to induce 100% estrus rates and 100% pregnancy rates.

Efisiensi reproduksi pada sapi perah yang teridentifikasi Bakteri non spesifik dalam saluran reproduksi Winda Nurul Lailatil Qodri; Sri Mulyati; Sri Pantja Madyawati; Rimayanti Rimayanti; Suherni Susilowati; Wiwiek Tyasmingsih
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 2 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i2.2020.41-47

The purpose of this study was to determine the reproductive efficiency of dairy cows in the presence of non-specific bacteria in the reproductive tract. This study was a descriptive study of 20 healthy and cycling dairy cows. The identification of non-specific bacteria in the reproductive tract was conducted in the previous study. Data were collected from interview, questionaire, and direct observation at the beginning of the study. Detection of estrus was performed visually, followed by artificial insemination 12-18 hours later. Non-return to estrus was checked after 21 days. Artificial insemination was repeated up to three times on cows returning to estrous. Pregnancy was checked through rectal palpation 60 days after the last insemination. Pregnant cows were followed until calving. The results showed that the estrus rate, non-return rate (NRR), conception rate (CR), calving rate (CvR), services per conception (S/C), and days open (DO), were respectively 100%, 85%, 85%, 85%, 1.3, and 93 days. It could be concluded that the presence of non-specific bacteria in the reproductive tract of dairy cows did not affect the reproductive efficiency so that pregnancy and calving could still occur.

Efektivitas pemberian GnRH pada sapi perah yang mengalami hipofungsi ovarium terhadap waktu timbulnya birahi dan angka kebuntingan Winadya Reika Ummaisyah; Sri Pantja Madyawati; Rimayanti Rimayanti; Wurlina Wurlina; Tjuk Imam Restiadi; Retno Sri Wahjuni
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.64-68

Ovarian hypofunction is a condition in which the follicles in the ovary can not develop due to lack of feed. It can affect the pituitary anterior, so the production of FSH and LH was low. GnRH can stimulate the pituitary anterior to produced FSH and LH. This study aimed to prove that GnRH injection in dairy cows with ovarian hypofunction can initiate estrus, followed by pregnancy. This study used 12 dairy cows with BCS ≥ 2.75, with anestrus due to ovarian hypofunction. All dairy cows were divided into two treatment groups, P1 injected with GnRH at a dose of 100 µg intramuscularly and P2 injected with GnRH at a dose of 300 µg intramuscularly. The results were analyzed using an independent t-test and Chi-Square test. The result showed that estrus rate of P1 and P2 were both 100%, meanwhile onset of estrus of P2 (53.83 ± 8.1 h) was shorter p <0.05) than P1 (74.17 ± 4.7 h). Both of P1 and P2 showed 100% pregnancy rate. It could be concluded that GnRH injection could initiate 100% estrus, and followed by 100% pregnancy in dairy cows with ovarian hypofunction.

Pengaruh pemberian ekstrak daun kelor (Moringa oleifera Lam.) terhadap motilitas dan viabilitas spermatozoa tikus (Rattus norvegicus) dengan paparan panas Arinda octavia; Budi Utomo; Sri Pantja Madyawati; Tri Wahyu Suprayogi; Agus Sunarso; Iwan Sahrial Hamid
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.65-71

This study aimed to determine the effect of Moringa (Moringa oleifera Lam.) leaf extract on the viability and motility of spermatozoa of rats (Rattus norvegicus) exposed to heat (40 ºC). A total of 25 rats aged 2.5 months and weighed 200 grams were randomly divided into five groups. Rats in K (–) group were not exposed to heat and without Moringa leaf extract administration. Rats in K (+) group were exposed to heat (40 ºC) for 1 hour daily without Moringa leaf extract administration. Meanwhile, rats in P1, P2, and P3 groups were administrated with Moringa leaf extract 100, 200, and 400 mg/kg BW/day, then exposed to 40 ºC 1 hour daily for 14 days. The results showed that exposure to 40 °C one hour per day for 14 days in K (+) group caused a decrease (p <0.05) on viability and motility of rat spermatozoa compared to those of the K (–) group. The administration of Moringa leaf extract (P1, P2, and P3 groups) was followed by an increase (p <0.05) in viability and motility of rat spermatozoa compared to the K (+) group. Viability and motility of rat spermatozoa in the P3 group (given Moringa leaf extract 400 mg/kg BW/day and exposed to 40 ºC 1 hour daily for 14 days) were not significantly different (p >0.05) compared to the viability and motility of rat spermatozoa in K (–) group. It could be concluded that administration of Moringa leaf extract 400 mg/kg BW/day can maintain the viability and motility of spermatozoa in white rats exposed to heat.

Comparison of reproductive performance of Ongole cross and Ongole-Limousin cross in Bekri district, Central Lampung regency, Lampung province, Indonesia Sukma, Puspa Permata; Ismudiono Ismudiono; Sri Mulyati; Pudji Srianto; Sri Pantja Madyawati; Ira Sari Yudaniayanti; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.9-17

This study aims to compare the reproductive performance of the Ongole cross (PO) and Ongole-Limousin cross (Limpo) on smallholder farmer in Bekri district, Central Lampung regency, Lampung province, a Indonesia. This study used 30 PO cows, 30 Limpo F1 (first filial) cows and 30 Limpo F2 cows. Sample cows had at least two parities and had never had a history of reproductive disorders. This study used primary and secondary data related to sample cows. Primary data was obtained from interviews with farmers and secondary data was obtained from the Department of Plantations, Livestock and Fisheries of Central Lampung regency, and inseminator recording. Data were described descriptively and analyzed using a one-way Analysis of Variance in Statistical Product and Service Solution (SPSS) version 23. The result showed that the services per conception of Limpo F2 cows was higher (p <0.05) than for PO and Limpo F1 cows. Days open and calving interval for Limpo cows (F1 and F2) were longer (p <0.05) than for PO cows. Days open and calving interval for Limpo F1 cows were not significantly different (p >0.05) than for Limpo F2 cows. Reproductive performance of PO cows was better compared to Limpo F1 and Limpo F2 cows. However, there was a data discrepancy between reproductive performance and data on rearing systems, feed quality, and signs of estrus observed by farmers. It could be concluded that PO cows had better reproductive performance than Limpo cows.

Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen Aditya Irawan; Fajar Ramadhan Subiyantoro; Suherni Susilowati; Tri Wahyu Suprayogi; Sri Pantja Madyawati; Pudji Srianto; Nove Hidajati; Eduardus Bimo Aksono Herupradoto; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.161-170

This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing.

Co-Authors A.T.Soelih Estoepangestie Abdul Samik Abdullah Hasib Aditya Irawan Agung Budianto Achmad Al arif, Mohammad Anam Arinda octavia Badri Munir Sukoco BUDI UTOMO Cindy Ercha Aulia Putri Dadik Rahardjo Dimas Kunto Satrio Dinda Reisinta Djoko Legowo Eduardus Bimo Aksono Herupradoto Erick Fajar Subhekti ERMA SAFITRI Fajar Ramadhan Subiyantoro Fedik Abdul Rantam Frida Aulya Arningdiah Gandul Atik Yuliani Hani Plumeriastuti Hermin Ratnani Herry Agoes Hermadi Ika WAHYUNI Indah Norma Triana Ira Sari Yudaniayanti Ismudiono Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Kevin Mardotillah Linda Sutanto Mayrena Ayu Cesaria Meles, Dewa Ketut Mufasirin Muhammad Al-Syafiq bin Abdul Halim Muhammad Aulia Rahman M’ Izi Kumala Lazuardi Sultoni Nenny Harijani Nove Hidajati Novia Alfi Masruro Nuurin Ajrin Karim Pudji Srianto Rahmatullah, Aldin Akbar Retno Sri Wahjuni Ricko Nurdianto S. Rimayanti Rimayanti . Rimayanti R Rimayanti Rimayanti Soeharsono Soeharsono Sri Hidanah Sri Mulyati Suherni Susilowati Sukma, Puspa Permata Sunarso, Agus Sunaryo Hadi Warsito Suzanita Utama Tantri Nawangwulan Tasya Apritalia Putri Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Widjiati . Widjiati W Widjiati Widjiati Widodo, Oky Setyo Widya Paramita L Winadya Reika Ummaisyah Winda Nurul Lailatil Qodri Wiwiek Tyasmingsih Wurlina, W Zahrorona, Amira Halimah

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