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All Journal Media Kedokteran Hewan Airlangga Development Journal Ovozoa: Journal of Animal Reproduction Indian Journal of Forensic Medicine & Toxicology Jurnal Kedokteran Hewan Frontiers in Community Service and Empowerment

Sri Pantja Madyawati

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Author-ID : 2019316

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Electrical & Electronics Engineering Engineering Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Comparison of reproductive performance of Ongole cross and Ongole-Limousin cross in Bekri district, Central Lampung regency, Lampung province, Indonesia Sukma, Puspa Permata; Ismudiono Ismudiono; Sri Mulyati; Pudji Srianto; Sri Pantja Madyawati; Ira Sari Yudaniayanti; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.9-17

This study aims to compare the reproductive performance of the Ongole cross (PO) and Ongole-Limousin cross (Limpo) on smallholder farmer in Bekri district, Central Lampung regency, Lampung province, a Indonesia. This study used 30 PO cows, 30 Limpo F1 (first filial) cows and 30 Limpo F2 cows. Sample cows had at least two parities and had never had a history of reproductive disorders. This study used primary and secondary data related to sample cows. Primary data was obtained from interviews with farmers and secondary data was obtained from the Department of Plantations, Livestock and Fisheries of Central Lampung regency, and inseminator recording. Data were described descriptively and analyzed using a one-way Analysis of Variance in Statistical Product and Service Solution (SPSS) version 23. The result showed that the services per conception of Limpo F2 cows was higher (p <0.05) than for PO and Limpo F1 cows. Days open and calving interval for Limpo cows (F1 and F2) were longer (p <0.05) than for PO cows. Days open and calving interval for Limpo F1 cows were not significantly different (p >0.05) than for Limpo F2 cows. Reproductive performance of PO cows was better compared to Limpo F1 and Limpo F2 cows. However, there was a data discrepancy between reproductive performance and data on rearing systems, feed quality, and signs of estrus observed by farmers. It could be concluded that PO cows had better reproductive performance than Limpo cows.

Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen Aditya Irawan; Fajar Ramadhan Subiyantoro; Suherni Susilowati; Tri Wahyu Suprayogi; Sri Pantja Madyawati; Pudji Srianto; Nove Hidajati; Eduardus Bimo Aksono Herupradoto; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.161-170

This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing.

Uterine prolapse and related factors in beef cow at the Besuki Animal Health Center, Situbondo regency, East Java, Indonesia Kevin Mardotillah; Suzanita Utama; Erma Safitri; Herry Agoes Hermadi; Sri Pantja Madyawati; Djoko Legowo; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.146-152

This study aims to determine the factors related to uterine prolapse in beef cow in the Besuki Animal Health Center working area in Situbondo regency. The sample consisted of 100 cows that calved in 2020-2022, whose data was obtained from the Department of Animal Husbandry of Situbondo regency, East Java, Indonesia. Determination of the months of the rainy and dry season was obtained from the Meteorological, Climatological and Geophysical Agency, Juanda Class I Meteorological Station. Other data regarding breed, age, parity of cow and dystocia incidence in cow was obtained by conducting interviews with farmers followed by confirmation with inseminator records, and direct observation of the housing system. The collected data were cross-tabulated and analyzed using Chi-square analysis to determine possible factors causing uterine prolapse. The results showed that the incidence of uterine prolapse in beef cow in the working area was 35% (35/100). The factors of housing, age, parity, and incidence of dystocia were significantly related (p <0.05) to the incidence of uterine prolapse, while breed and season did not have a significant influence (p > 0.05). It could be concluded that the age and parity of the cow at the time of calving, the practice of housing cow, and the incidence of dystocia were related to the incidence of uterine prolapse in cows in the Besuki Animal Health Center operational area.

Estrus quality, non-return rate and conception rate in the incidence of repeat breeding in dairy cow after 2%povidone iodine therapy combined with prostaglandin F2α at KUD Tani Wilis Tulungagung Zahrorona, Amira Halimah; Sri Pantja Madyawati; Pudji Srianto; Hermin Ratnani; Sunaryo Hadi Warsito; Sri Hidanah
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 1 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i1.2025.46-51

Repeat breeding is a condition in which cows fail to conceive after being bred three or more times. This study aims to determine the quality of estrus, non-return rate (NRR) and conception rates obtained after cows were treated with 2% povidone iodine. The study involved 30 Holstein Friesian cows, each having calved three times and experiencing repeat breeding. Cows in estrus were treated with 10 mL of 2% povidone iodine intrauterinely, followed by an intramuscular injection of 2 mL (250 mg/head) of prostaglandin F2α (PGF2α, Luteosyl) five days later. Estrus quality was observed by examining changes in vulvar mucosa color, mucus discharge, and vulvar temperature. Pregnancy diagnosis was made at 60 days. Results showed that 43.3% of cows had estrus quality score 3 (cows showed dark red vulvar mucosa, transparent mucus, temperature 38.9 ± 0.5 ºC), 23.3% had score 2 (cows showed reddish vulvar mucosa, some cloudy mucus, temperature 38.7 ± 1.0 ºC), and 33.3% had score 1 (cows showed pink vulvar mucosa, cloudy mucus, temperature 38.6 ± 0.5 ºC). Of the 30 cows, 20 had a non-return rate (NRR) of 66.7%. Based on these results, it can be concluded that 2% povidone iodine has a positive effect on estrus quality, NRR, and conception rates in dairy cows experiencing repeat breeding.

Factors associated with repeat breeding in Holstein Friesian cows at the Tani Wilis Village Cooperative Unit, Sendang District, Tulungagung Regency, Indonesia Dimas Kunto Satrio; M’ Izi Kumala Lazuardi Sultoni; Tasya Apritalia Putri; Soeharsono Soeharsono; Dadik Rahardjo; Djoko Legowo; Gandul Atik Yuliani; Iwan Sahrial Hamid; Mohammad Anam Al-Arif; Sunaryo Hadi Warsito; Pudji Srianto; Sri Pantja Madyawati; Tita Damayanti Lestari; Wurlina Wurlina
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 2 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i2.2025.67-75

This study aimed to evaluate the association of factors such as nutrition, thermal humidity index (THI), age, and lactation period with the occurrence of repeat breeding of dairy cows. The sample consisted of Holstein Friesian cows aged 1-11 years, with lactation periods ranging from 1 to 7 months, currently in lactation, and experiencing repeat breeding (inseminated three or more times without conception, despite exhibiting normal estrous cycles and intervals). The results showed that feeding practices by farmers did not significantly differ (p >0.05) in relation to the incidence of repeat breeding. THI values recorded in several barns also showed no significant differences (p >0.05), with the cows experiencing light to moderate heat stress. Similarly, the frequency of artificial insemination and the estrous cycle intervals remained within the normal range. The highest incidence of repeat breeding was observed in cows aged 2.5 to 5.5 years, accounting for 78.13%, and during lactation periods of 1-3 months, accounting for 81.26%. It can be concluded that there were no significant differences in the provision of forage and leguminous feed between normal cows and those with repeat breeding, and the crude protein content of forage did not influence its occurrence. Additionally, there was no indirect relationship between THI and repeat breeding. However, the incidence of repeat breeding tended to increase in cows aged 3-4 years, and during the second and third lactation periods.

Effect of fruit juices in skim milk extender in maintaining Sapudi ram spermatozoa quality at chilled temperature Retta, Ayun Tria Marga; Suherni Susilowati; Sri Pantja Madyawati; Tatik Hernawati; Wurlina Wurlina; Retno Sri Wahjuni
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 2 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i2.2022.49-53

This study aimed to determine the effect of the addition of cherry tomato, california papaya, and honey melon juice into skim milk extender in maintaining the quality of Sapudi ram semen at chilled temperature (5oC) storage. Five replication of ejaculates were divided equally into four groups. In control group (T0) semen was diluted in skim milk extender, while in T1, T2, and T3 groups semen were diluted in skim milk extender contained 20% of cherry tomato, california papaya, and honey melon juice, respectively. The extended semen was stored at a chilled temperature, and semen quality (based on sperm motility, viability, and plasma membrane integrity) was observed daily for five days. The result showed that semen quality declined day by day during the five days of storage. Based on the minimum standard of post-thawed semen motility (40%), the spermatozoa of the control group only lasted by the second day. Meanwhile, in the groups with the addition of fruit juice motility could last up to the third day, with the highest motility (p <0.05) on the addition of california papaya juice. This study concluded that the addition of 20% (v/v) california papaya juice in skim milk extender could maintain the percentage of spermatozoa motility up to three days at a chilled temperature.

Combination of 5% Dextrose Ringer's solution and egg yolk extender maintained the motility and viability of kampung rooster spermatozoa in chilled temperature Ahmad Hanif Azzam; Dadik Rahardjo; Tatik Hernawati; Sri Pantja Madyawati; Budi Utomo
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.109-114

This study aims to determine the motility and viability of kampung rooster (Gallus domesticus) spermatozoa in 5% Dextrose Ringer's solution as an extender with or without the addition of various egg yolk concentrations at 5ºC during storage. Semen was collected by massage in the morning. In this study, four types of extenders were used, i.e., 5% Dextrose Ringer's extender without egg yolk, 5% Dextrose Ringer's extender with 5, 7.5, and 10% egg yolk for T0, T1, T2, and T3, respectively. Then, 1000 IU Penicillin and 1 mg Streptomycin were added per mL extender. Fresh semen from each rooster was evenly divided into four volumes to be diluted 11 times in each group extender. Extended semen from all groups was stored at 5°C. Data were analyzed using ANOVA and Duncan's new multiple range test. The result showed a significant difference (p <0.05) between each treatment group for spermatozoa motility and viability. In conclusion, this study revealed that the addition of various concentrations of egg yolk into 5% Dextrose Ringer's solution as an extender maintained the motility and viability of kampung rooster spermatozoa.

Green tea (Camellia sinensis) leaf extract maintained spermatozoa plasma membrane integrity, viability, and motility of mice (Mus musculus) exposed to cigarette smoke Maryana Siska Silviani; Moh. Sukmanadi; Rochmah Kurnijasanti; Sri Pantja Madyawati; Epy Muhammad Luqman
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.130-136

Cigarette smoke chemicals caused oxidative stress by increasing Reactive Oxygen Species (ROS). ROS exposure could be neutralized by antioxidants, such as green tea (Camellia sinensis) leaf extract. This study aimed to determine the effect of green tea extract (GTE) on spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice (Mus musculus) exposed to cigarette smoke. Twenty-five mice were randomly divided into five groups. Group C- mice were given a placebo (1% Sodium carboxymethyl cellulose, Na-CMC). Group C+, T1, T2, and T3 mice were exposed to cigarette smoke and given 0, 20, 40, and 60 mg/kg BW GTE respectively. Cigarette smoke exposure used a clove cigarette per day. GTE in 1% Na-CMC solution was administered at 0.5 mL orally using a gastric probe. The treatment was conducted daily for 36 days, and on day 37, all mice were euthanized for spermatozoa evaluation. The results showed that all parameters evaluated in the C+ group were lower (p <0.05) than in C- group. Administration of GTE in the T2 group increased (p <0.05) all parameters compared to mice in the C+ group. However, administration of GTE to mice in the T3 group caused a decrease (p <0.05) in all parameters than those of the T2 group and was not significantly different (p >0.05) compared to those of the C+ group. It could be concluded that the administration of GTE at 40 mg/kg BW has maintained the spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice exposed to cigarette smoke.

Retained placenta in dairy cows living in an all-day cowshed rearing system Sayi Datur Rohmah; Hermin Ratnani; Sunaryo Hadi Warsito; Rimayanti Rimayanti; Sri Pantja Madyawati; Sri Mulyati; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.71-80

This study aimed to determine the prevalence and factors that influence the incidence of retained placenta in dairy cows reared under an all-day cowshed housing system at KUD Tani Wilis, Sendang District, Tulungagung. This study used 216 dairy cows, that had already calved, that had been identified to have medical and reproductive records for data exploration. Data collected included the sex and weight of calf, single or twin calves, age of the dam, cowshed structure (floor, puddles on the floor, gutter, distance of cowshed to waste disposal, type of floor and roof, and sunlight exposure), feed of the dam (forage, concentrate, mineral supplements), frequency of retained placenta cases on calving intervals and days open of the dams. The prevalence of retained placenta was presented descriptively. Factors related to the retained placenta and its effect on calving interval and days open were analyzed by Chi-square test using Statistical Product and Service Solution (SPSS) software version 20 for Windows. The result showed that the prevalence rate of retained placenta was 19.91% of all dairy cow populations in the district. Furthermore, the sex of the calf, age of the dam, sunlight exposure, and the quantity of feed (forage and concentrate) were related to the retained placenta (p <0.05). In addition, retained placenta increased along with extending of days open and calving interval. It could be concluded that the factors that influenced the prevalence of retained placenta were the sex of the calf, the age of the cow, the feed and concentrate, and the cowshed that is not facing the sun.

Comparative effects of epigallocatechin-3-gallate supplementation in skim milk, egg yolk-citrate, and combined extenders on the spermatozoa quality of Ettawah crossbred bucks during storage at 5 °C Muhammad Ikmal Robih; Anas Bad’rosana Prawindi; Tatik Hernawati; Budi Utomo; Suherni Susilowati; Tri wahyu Suprayogi; Sri Pantja Madyawati; Kadek Rachmawati; Mustofa Helmi Effendi; Yeni Dhamayanti
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.147-158

This study aimed to determine the quality of Ettawah crossbred buck semen diluted in skim milk, egg yolk-citrate extenders, or their combination, supplemented with epigallocatechin-3-gallate (EGCG). Semen was collected from Ettawah crossbred bucks using an artificial vagina. Six ejaculates were used for replication and divided into three extender treatments: T1, skim milk; T2, egg yolk-citrate; and T3, a combination of both, each supplemented with 1.5 µg/mL EGCG. The extended semen was stored in a refrigerator at 5 °C. Diluted samples were evaluated every 24 hours until spermatozoa motility declined to 30%, the minimum requirement for artificial insemination. The results showed that spermatozoa motility, viability, plasma membrane integrity, and morphological abnormality decreased during six days of storage at 5 °C. Spermatozoa motility remained above 30% for five days in T1 (36.50±1.22%) and T2 (41.67±2.06%), and for six days in T3 (43.33±1.03%). On day six, semen in the combined extender supplemented with EGCG (T3) showed significantly higher motility, viability, and membrane integrity, and lower morphological abnormalities (p <0.05) than the other treatments. In T2, all spermatozoa quality parameters were higher (p <0.05) than in T1. It can be concluded that the combined skim milk-egg yolk-citrate extender containing 1.5 µg/mL EGCG best maintained the spermatozoa quality of Ettawah crossbred bucks during six days of storage at 5 °C. Based on spermatozoa motility, the semen remained suitable for artificial insemination.

Co-Authors A Ashiila Tsabita Abdullah Hasib Aditya Irawan Agung Budianto Achmad, Agung Budianto Ahmad Hanif Azzam Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Anas Bad’rosana Prawindi Badri Munir Sukoco BUDI UTOMO Candra Panji Asmoro, Candra Panji Cindy Ercha Aulia Putri Dadik Rahardjo Dimas Kunto Satrio Djoko Legowo Epy Muhammad Luqman Erick Fajar Subhekti ERMA SAFITRI Fachry Abda El Rahman Fajar Ramadhan Subiyantoro Fedik Abdul Rantam Frida Aulya Arningdiah Gandul Atik Yuliani Hariyono Hariyono Hermin Ratnani Herry Agoes Hermadi Hidajati, Nove Ika WAHYUNI Indira, Sitti Az-Zahra Ira Sari Yudaniayanti Ismudiono Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Kevin Mardotillah Kusnoto Kusnoto, Kusnoto Legowo, Djoko Leo Yosdimyati Romli Linda Sutanto Maryana Siska Silviani Moh. Sukmanadi Mufasirin Muhammad Al-Syafiq bin Abdul Halim Muhammad Aulia Rahman Muhammad Ikmal Robih Mustofa Helmi Effendi M’ Izi Kumala Lazuardi Sultoni Nove Hidajati Nuurin Ajrin Karim Pudji Srianto Rahmatullah, Aldin Akbar Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti . Rimayanti R Rimayanti Rimayanti Risanti Handayani Rochmah Kurnijasanti Sayi Datur Rohmah Soeharsono Soeharsono Sri Hidanah Sri Mulyati Suherni Susilowati Sukma, Puspa Permata Suzanita Utama Tasya Apritalia Putri Tatik Hernawati Teguh Hari Sucipto, Teguh Hari Tita Damayanti Lestari Tri Wahyu Suprayogi Trilas Sardjito Warsito, Sunaryo Hadi Widjiati . Widjiati W Widjiati Widjiati Widodo, Oky Setyo Wurlina, W Yeni Dhamayanti Zahrorona, Amira Halimah

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