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Ysrafil, Ysrafil
Departemen Farmakoterapi, Fakultas Kedokteran, Universitas Palangka Raya
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Antibacterial Activity of Pasak Bumi Stem (Eurycoma longifolia J.) Extract against Salmonella typhi Sirait, Christine; Hanasia, Hanasia; Martani, Natalia Sri; Ysrafil, Ysrafil; Fatmaria, Fatmaria
Majalah Obat Tradisional Vol 29, No 2 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.91168

Abstract

Typhoid fever is caused by consuming food or water contaminated with Salmonella typhi. The disease develops from bacterial infection through the consumption of contaminated sustenance and drink. The bacterium can cause bacteremia, which is bacteria living in the blood, penetrating the mucosal epithelium of the small intestine and entering the lymphatic flow. Therefore, this study aimed to assess the potential inhibitory effect of ethanol extracts derived from stems of Eurycoma longifolia (pasak bumi) on the growth of Salmonella typhi bacteria. Compounds such as alkaloids, saponins, flavonoids, terpenoids, and tannins in pasak bumi stems possessed antibacterial properties. Extracts were made using 96% ethanol at varying concentrations (10%, 20%, 30%, 40%, 50%) with distilled water and chloramphenicol as negative and positive control. The result showed that pasak bumi stem extracts inhibited Salmonella typhi, with increasing efficacy at higher concentrations and statistical analysis reported significant differences between all treatment groups (p<0.001). Average zone diameter was 0 mm and 23.10 mm for negative and positive control, as well as 2.75 mm, 4.10 mm, 5.24 mm, 6.98 mm, and 8.55 mm for 10%, 20%, 30%, 40% and 50% extracts, respectively. This study provided verification of antibacterial effects of pasak bumi stem ethanol extracts against Salmonella typhi.
PELATIHAN PEMBUATAN MIE KAYA SERAT-PROTEIN DARI UBI JALAR DAN IKAN SELUANG SEBAGAI UPAYA PENCEGAHAN STUNTING Yuliana Yuliana; Hanasia Hanasia; Ysrafil Ysrafil; Muh. Supwatul Hakim; Dwi Hermayantiningsih
JMM (Jurnal Masyarakat Mandiri) Vol 9, No 1 (2025): Februari
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v9i1.27789

Abstract

Abstrak: Desa Jabiren menjadi lokus stunting tertinggi di Kabupaten Pulang Pisau mencapai angka 31,6%, lebih tinggi dari rata-rata prevalensi stunting di Kota Palangka Raya (27,8%) dan Provinsi Kalimantan Tengah (26,9%). Kurangnya pemahaman orang tua terhadap asupan gizi balita dan faktor-faktor mencetus stunting berkontribusi atas kejadian 26 balita mengalami stunting dari total 80 balita di Desa Jabiren. Kegiatan ini bertujuan untuk memberikan pelatihan pengolahan pangan lokal yaitu ubi jalar dan ikan seluang menjadi inovasi pangan berupa Mie Kaya Serat-Protein (KASEIN). Peserta kegiatan berjumlah 40 orang dari kader PKK dan kader posyandu. Kegiatan ini dilaksanakan mulai dari persiapan, sosialisasi stunting, pelatihan membuat mie, dan evaluasi. Evaluasi menggunakan kuesioner menunjukkan bahwa peserta sangat setuju (68,33%) dan setuju (31,67%) kegiatan ini dilakukan. Pengetahuan peserta terkait stunting juga meningkat hingga 93,60% dan pelatihan mengolah pangan lokal menjadi Mie KASEIN berhasil dilakukan. Nilai uji organoleptik mie memperoleh 51,7% (sangat suka) dan 48,3% (suka). Peserta diharapkan terus berinovasi mengolah pangan lokal sebagai upaya pencegahan stunting.Abstract: Jabiren Village has the highest stunting prevalence in Pulang Pisau District at 31.6%, higher than the average stunting prevalence in Palangka Raya City (27.8%) and Central Kalimantan Province (26.9%). Parents' lack of understanding of toddler nutrition and the factors that trigger stunting contributed to the incidence of 26 stunted toddlers out of a total of 80 toddlers. This project aims to provide training in processing local food, namely sweet potato and seluang fish into food innovations in the form of Fiber-Protein Rich Noodles. There were 40 participants from PKK and Posyandu cadres. The activity begins with preparation, socialization of stunting, training in making noodles, and evaluation. Questionnaire evaluation showed that participants strongly agreed (68.33%) and agreed (31.67%) with project implementation. Participants' knowledge of stunting also increased to 93.60% and the training was successfully. The organoleptic test value of the noodles was 51.7% (very like) and 48.3% (like). Participants are expected to keep innovating in processing local food to prevent stunting.
Evaluation of the combination patch of betel leaf extract (Piper sp.) - ultrasonic assisted extraction as a transdermal delivery system on fibroblast cell formation and collagen density Valencia, Regina; Trinovita, Elsa; Shinta, Herlina Eka; Toemon, Angeline Novia; Ysrafil, Ysrafil
JURNAL ILMU KEFARMASIAN INDONESIA Vol 23 No 1 (2025): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v23i2.1687

Abstract

The combination of red betel (Piper crocatum) and green betel (Piper betle L.) leaves is believed to act synergistically in enhancing wound healing. Transdermal patches offer advantages over conventional topical forms due to their sustained drug release, ease of application, and prolonged therapeutic effect. This study evaluated the histopathological effects of a combination patch containing betel leaf extracts for incision wound treatment in Wistar rats. The studies used an true experimental post-test-only control group design, five groups were tested: a blank patch (K−), a povidone-iodine patch (K+), and combination extract patches at concentrations of 7.5% (K1), 15% (K2), and 30% (K3), applied over 14 days with six animals per group. Patch characteristics were assessed through organoleptic tests, thickness, weight uniformity, folding endurance, and pH, while wound healing was evaluated through histological analysis of fibroblast cell counts and collagen density. Statistical analysis was conducted using the Kruskal-Wallis test followed by Mann-Whitney post-hoc tests. The 30% combination patch (K3) showed the most significant healing effect, with fibroblast counts exceeding 50 cells and dense collagen formation scoring +3. These results indicate that a transdermal patch combining Piper crocatum and Piper betle L. extracts effectively promotes wound healing by enhancing fibroblast proliferation and collagen synthesis.
Antioxidant Potential of Secondary Metabolite from Kelakai (Stenochlaena palustris) as a Natural Growth Promoter Through Inhibition of Inducible Nitrite Oxide Synthase (iNOS) Irmawan, Muhammad; Kumalasari, Meiyanti Ratna; Sandriya, Ardi; Fariz Noorrahman, Nabil; Ysrafil, Ysrafil; Tesalonika, Lidya
The Journal of Pure and Applied Chemistry Research Vol. 14 No. 1 (2025): Edition January-April 2025
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2025.014.01.6534

Abstract

Kelakai (Stenochlaena palustris), a plant native to Central Kalimantan, is recognized for its potent antioxidant properties. This study aimed to investigate the preparation, extraction, phytochemical composition, antioxidant potential, and molecular interactions of secondary metabolites derived from Kelakai, emphasizing its health-promoting benefits and possible use as a natural growth enhancer. The findings revealed that the Kelakai extract contained various secondary metabolites, including alkaloids (8.868%), flavonoids (5.3%), saponins (10.76%), phenolics (1.246%), tannins (0.067%), and triterpenes (30.7%). The extract demonstrated a powerful antioxidant effect, with an IC50 value of 44.81 μg/mL. Molecular docking analysis identified chlorogenic acid and eucalyptol as essential compounds contributing to the extract's antioxidant activity. These compounds effectively inhibit the iNOS protein, with binding free energy (ΔGbinding) values of -10.3044 kcal/mol and -6.0872 kcal/mol, respectively. These findings suggest that kelakai extract possesses significant antioxidant potential and may offer valuable applications as a natural growth promoter for broiler chickens.
Comprehensive In Silico Analysis Of Bioactive Compounds From Pyrrosia piloselloides Leaves As Xanthine Oxidase Inhibitors Sinuhaji, Masira Sony Crespo; Havid, Husnul; Ysrafil, Ysrafil; Alexandra, Francisca Diana
Pharmacology and Clinical Pharmacy Research Vol 10, No 2 (2025)
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15416/pcpr.v10i2.60233

Abstract

This study aimed to evaluate the potential of bioactive compounds from Pyrrosia piloselloides leaves as xanthine oxidase (XO) inhibitors through an in silico approach. Methods included molecular docking and molecular dynamics simulations to identify the binding affinity of test compounds to XO. Among 59 screened compounds, 3 compounds (2,6,10,14,18,22-Tetracosahexaene, 6-Hydroxymethaqualone, and β-Sitosterol acetate) exhibited the lowest binding energy in comparison to the control, Allopurinol, with key residue contributions from GLU 802, ARG 880, and THR 1010. Molecular dynamics simulations confirmed the interaction stability, with β-Sitosterol acetate showing the lowest total binding free energy (-46.23 ± 0.66 kcal/mol). ADMET predictions revealed promising pharmacokinetic profiles for these compounds. This study highlights Pyrrosia piloselloides as a promising source of novel XO inhibitors, with further validation required through in vitro and in vivo experiments.
Pengaruh konsentrasi ekstrak daun pare (momordica charantia l.) terhadap kematian larva aedes aegypti Halim, Rahmawati; Toemon, Agnes Immanuela; Ysrafil, Ysrafil; Fatmaria, Fatmaria; Turnip, Oktaviani Naulita
Jurnal SAGO Gizi dan Kesehatan Vol 6, No 2 (2025): Agustus
Publisher : Poltekkes Kemenkes Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/gikes.v6i2.2320

Abstract

Background: Dengue Hemorrhagic Fever (DHF) is a health problem in Indonesia, transmitted by the Aedes aegypti mosquito. Bitter melon leaves contain active compounds such as saponins, alkaloids, and flavonoids belived to be effective as larvacides and are expected to serve as a more environmentally friendly alternative for mosquito control in preventing the spread of DHF.Objective: To determine the potential of natural biolarvacide from bitter melon leaf extract (Momordica charantia L.) against Aedes aegypti larvae.Methods: An experimental study was conducted at the Public Health Laboratory of Tanah Bumbu in July 2024. This experimental research used 375 Aedes aegypti larvae with five treatments and three repetitions, observing the number of larvae that died after 24 hours. The data were analyzed using the Shapiro-Wilk test, Levene's test, Kruskal-Wallis test, Mann-Whitney test, and probit analysis.Results: Bitter melon leaf extract at concentrations of 0,3% and 0,6% did not cause larval death, so it was not effective in inhibiting the growth of Aedes aegypti. While at concentrations of 0,9% showed a significant effect in inhibiting larval growth. The results of the Kruskal-Wallis test on the death of larvae treated with bitter melon leaf extract obtained a significance value of 0,008 (p <0,05). The results of the Mann-Whitney test showed that there was a significant difference between the concentration of 0,9% bitter melon leaf extract and the positive control abate, as well as between the negative control aquadest and the concentration of 0,9% bitter melon leaf extract. The results of the probit analysis test obtained an LC50 value of 1,202%.Conclusion: The 0.9% concentration of bitter melon leaf extract can inhibit the growth of Aedes aegypti larvae. However, it is not yet suitable as an alternative bio-larvicide.
The Cytotoxicity of Endophytic Fungi Isolated from Kelakai (Stenochlaena palustris) Against MCF-7 and Vero Cells Dali; Mashar, Harlyanti Muthma’innah; Damiti, Sukmawati Ahmad; Ysrafil, Ysrafil
Public Health of Indonesia Vol. 11 No. 3 (2025): July - September
Publisher : YCAB Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36685/phi.v11i3.1002

Abstract

Background: The high prevalence of cancer has a direct impact on the quality of life and nutritional status of sufferers, mainly due to decreased appetite, metabolic disorders, and side effects of conventional therapies such as surgery, mastectomy, chemotherapy, hormonal therapy, or radiation therapy. These therapies, although aimed at inhibiting the growth and metastasis of cancer cells, often cause severe side effects that worsen the patient's condition. Therefore, it is important to explore alternative treatments that are effective and have minimal side effects. In this regard, biological sources, such as endophytic fungi kelakai, contain various active secondary metabolites that can inhibit cancer cell proliferation so that they can be part of a more public health-friendly supportive therapy approach.Objective: This study aims to prove the cytotoxic activity of the extract of endophytic fungi in kelakai against MCF-7 and Vero cells.Methods: Isolation, purification, and extraction process of secondary metabolites from the endophytic fungi isolates were carried out in the same manner as our previous research. The ethyl acetate extract of kelakai was obtained by extractive fermentation from pure isolates of the endophytic fungi in kelakai. Extraction was carried out by the fractionation method using the ethyl acetate solvent at a ratio of 1:1. Identification of chemical contents, including tannins, alkaloids, flavonoids, saponins, and steroids/terpenoids, was conducted qualitatively. Cytotoxic activity was tested by the MTT method.Results: From the isolate extraction, 2.4 g of ethyl acetate extract was produced. The identification of chemical contents showed positive results, meaning that the extract did contain tannins, flavonoids, alkaloids, steroids, and saponins. The extract cytotoxicity test against MCF-7 and Vero cells yielded IC50 values of 517.3015 μg/ml and 1,074.9152 μg/ml, respectively. Conclusion: Based on these results, the extract of kelakai was not toxic to Vero cells, but it was toxic to MCF-7 cells. These findings suggest that the kelakai can be the basis for exploring local plants as a source of natural medicine while supporting the conservation of endemic plants to provide safer alternative therapies and improve public health. Keywords: Stenochlaena palustris; Endophytic Fungi; MTT; MCF-7; Vero cell
The Antivirulence Mechanisms of Phytate Against Pathogenic Bacteria in Skin Infections Zhofiroh, Nabilatul; Praja, Rian Ka; Trinovita, Elsa; Ysrafil, Ysrafil; Surbakti, Ranintha BR
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.731-738

Abstract

Skin infections caused by the bacteria Staphylococcus aureus, Streptococcus pyogenes, and Propionibacterium acnes are often a common health problem. One treatment is antibiotics, but the cases of antibiotic resistance are increasing. Thus, new treatment alternatives are needed. This study aimed to analyze the molecular mechanism of phytate antivirulence against pathogenic bacteria of skin infection. This study used a bioinformatics approach involving analysis of phytate interactions with bacterial virulent proteins via STITCH, functional classification of proteins with VICMpred, and prediction of virulence properties using VirulentPred. B-cell and MHC epitopes were analyzed using IEDB, while protein subcellular location was determined through PSORTb. The results showed that phytate interacted specifically with virulent proteins in all three bacteria, most of which functioned in cellular and metabolic processes. These virulent proteins also have immunologically relevant epitopes. Subcellular location analysis showed that phytate protein targets were dispersed in the cytoplasmic membrane and cytoplasm. These findings indicated that phytate has a significant antivirulence mechanism by targeting virulent proteins of skin pathogenic bacteria, thus potentially becoming a therapeutic agent to treat skin infections while reducing antibiotic resistance.
siRNAs targeting icaD Gene of Staphylococcus aureus to Inhibit Biofilm Formation: Structural Analysis and Efficacy Sulistina, Dinda Ananda; Praja, Rian Ka; Anugerahny, Margaretha Yayu Indah; Hanasia, Hanasia; Ysrafil, Ysrafil
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.921-926

Abstract

Antibiotic resistance in Staphylococcus aureus infections, especially those involving biofilm formation, is a global health issue. Biofilm protects bacteria from the immune system and antibiotic treatment, making them 10 to 1000 times more resistant. The icaD gene, part of the ica operon, is crucial for biofilm synthesis by enhancing the enzymes responsible for forming the biofilm matrix. The icaD gene sequence of Staphylococcus aureus was obtained from the GenBank NCBI database with the accession code CP140612.1, with a gene sequence length of 306 bp and employed several bioinformatics methods, including siDirect for designing and evaluating effective siRNA sequences to select the most promising candidates. Additionally, siRNA Scales, MaxExpect, Duplex Fold, and siPred were employed to analyze the siRNA sequence length, secondary structure, binding energy, and efficacy predictions of siRNAs targeting the icaD gene. The study found that out of 54 siRNA candidates, siRNA22, siRNA50, and siRNA25 achieved inhibition rates of 93.69%, 92.82%, and 92.52%, respectively. These results bioinformatically demonstrated their potential to suppress the expression of the icaD gene and highlight their promise as siRNA-based antibacterial therapies to combat biofilm-related infections. The designed siRNA computationally shows potential as an innovative therapy to combat biofilm infections caused by Staphylococcus aureus.
Aktivitas Antioksidan Ekstrak Etanol Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb) Menggunakan Metode DPPH: Antioxidant Activity of Ethanol Extract of Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb) Using DPPH Method Halisa, Nida; Frethernety, Agnes; Permatasari, Silvani; Alexandra, Francisca Diana; Ysrafil, Ysrafil
Jurnal Surya Medika (JSM) Vol. 10 No. 2 (2024): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsm.v10i2.6151

Abstract

Antioksidan dalam mendetoksifikasi radikal bebas akan ikut teroksidasi sehingga untuk mencukupi kebutuhan tubuh diperlukan antioksidan eksogen. Antioksidan eksogen dapat diperoleh dari tanaman herbal salah satunya Bajakah Kalalawit. Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb.) adalah salah satu jenis bajakah yang terdapat di Kelurahan Marang, Kalimantan Tengah yang berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk menganalisis aktivitas antioksidan dan kekuatan antioksidan Bajakah Kalalawit (Uncaria gambir (W. Hunter) Roxb.). Batang Bajakah Kalalawit diekstrak dengan metode maserasi 3x24 jam menggunakan pelarut etanol 96%. Setelah didapatkan ekstrak kental kemudian dianalisis kandungan senyawa metabolit sekunder dengan uji fitokimia. Uji aktivitas antioksidan dibuat dengan konsentrasi ekstrak 20ppm, 40ppm, 60ppm, 80ppm, dan 100ppm dengan metode DPPH (2,2-diphenyl-1-picrylhydrazyl). Hasil penelitian menunjukkan senyawa yang terdapat dalam ekstrak etanol Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb) adalah terpenoid, flavonoid, fenolik, steroid, saponin, alkaloid, dan tanin. Ekstrak etanol Bajakah Kalalawit memiliki aktivitas antioksidan yang sangat kuat dengan nilai IC50 8,69ppm.
Co-Authors Agnes Frethernety Ahmad Sukrianur Alayya Adistia Putri Alexandra, Francisca Diana Angeline Novia Toemon, Angeline Novia Anugerahny, Margaretha Yayu Indah Ardi Sandriya Dali Dali Damiti, Sukmawati A Damiti, Sukmawati Ahmad Denny Indra Setiawan Desyandri Desyandri Dwi Hermayantiningsih Elsa Trinovita Erwin Prasetya Toepak Erwin Prasetya Toepak Fariz Noorrahman, Nabil Fatmaria Fatmaria Fatmaria, Fatmaria Fihrina Mohamad Francisca Diana Alexandra Frethernety, Agnes Hakim, Muh. Supwatul Halisa, Nida Hanasia , Hanasia Hanasia Hanasia Hanasia, Hanasia Harlyanti Muthma'innah Mashar Hartati Hartati Hartati Hartati Havid, Husnul Indwiani Astuti Irmawan, Muhammad Ishak, Pratiwi Ismail Ismail Komara, Nisa Kartika Marvin Horale Pasaribu Mashar, Harlyanti Muthma’innah Miranti Maya Sylvani Muhammad Irmawan Natalia Sri Martani Oktriani R Permatasari, Silvani PRISCA SAFRIANI WICITA Rahman, Sudarman Rahmawati Halim Rahmawati, Rahmawati Ranintha br Surbakti Remi Ayu Pratika Rian Ka Praja Sari, Meiyanti Ratna Kumala Sa’adah N Sinuhaji, Masira Sony Crespo Sirait, Christine Sofia Mubarika Haryana Stevin Carolius Angga Sukmawati A. Damiti Sukmawati Damiti Sulistina, Dinda Ananda Syamsul Thalib Teguh Aryandono Tesalonika, Lidya Toemon, Agnes Immanuela Torizal GF Turnip, Oktaviani Naulita Valencia, Regina Vyani Kamba Wardana T Yuliana Yusuf, Ghaitsa Zahira Sopha Zaenal Abidin Zhofiroh, Nabilatul