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All Journal HAYATI Journal of Biosciences Jurnal Natur Indonesia AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL ILMU PERTANIAN Biogenesis: Jurnal Ilmiah Biologi CEMARA Journal of Degraded and Mining Lands Management Jurnal Keteknikan Pertanian Tropis dan Biosistem Jurnal Agro Planta Tropika JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrin : Jurnal Penelitian Pertanian AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA Jurnal Agrotek Indonesia Jurnal Abdi Insani Jurnal Ilmiah Agribios BIOVALENTIA: Biological Research Journal Journal of Applied Agricultural Science and Technology Journal of Soilscape and Agriculture PAPUMA: Journal of Comunnity Services Jurnal Natur Indonesia
Khozin, Mohammad Nur
Program Studi Agronomi, Fakultas Pertanian, Universitas Jember, Jawa Timur
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences

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Journal : BIOVALENTIA: Biological Research Journal

 1 
Porang (Amorphophallus muelleri B.) Indirect Organogenesis Nur Khozin, Mohammad; Ahmad Iqbal Rananda; Didik , Pudji Restanto
BIOVALENTIA: Biological Research Journal Vol. 10 No. 2 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.10.2.2024.334

Abstract

Organogenesis is regeneration mechanism on the process of organ formation derived from somatic or non-meristematic tissue on in vitro technique. Organogenesis can occur directly or indirectly. This research used porang leaves as explant, and medium MS Basal with addition such as sucrose, agar, combination of PGR’s 2,4 D and NAA, and aquadest. This research method used the Completely Randomize Design (CRD) with 9 different level combination of 2,4 D and NAA such as 2,0 ppm 2,4 D + 1,5 ppm NAA (A1), 2,0 ppm 2,4 D + 2,0 ppm NAA (A2), 2,0 ppm 2,4 D + 2,5 ppm NAA (A3), 2,5 ppm 2,4 D + 1,5 ppm NAA (A4), 2,5 ppm 2,4 D + 2,0 ppm NAA (A5), 2,5 ppm 2,4 D + 2,5 ppm NAA (A6), 3,0 ppm 2,4 D + 1,5 ppm NAA (A7), 3,0 ppm + 2,0 ppm NAA (A8), 3,0 ppm 2,4 D + 2,5 ppm NAA (A9) with 3 repetitions for each combination. Quantitative data such as the percentage of explants that appeared callus and roots were tested statistically by using ANOVA to determine the significance of the effect of the treatment. Treatments that had significant results were then tested using the DMRT test with a level of α=5%. Qualitative data were observed visually and analyzed descriptively. Combination concentration treatment of 2,4 D and NAA have the significant effect on callus and root formation. The level of 2 ppm 2,4 D and 2 ppm NAA is the best treatment on callus formation process in terms of 100% callus emerge and the early day appears at 24 days after planting and the best treatment on the process of root formation in terms of percentage roots emerge 100% and the early day roots appears at 46 days after planting. Indirect organogenesis is type of of root regeneration.
Co-Authors . Usmadi Agustin, Vira Audita Ahmad Iqbal Rananda Ahnaf, Yusuf Dary Aji, Seto Purnomo Alfarisy, Fariz Kustiawan Alfarizi, Zulfikar Ahmad Aultavia, Reza Novita Azmi, Hilmi Tsabiatul Azzahro, Firyal Nurul Haifa Bakhroini Habriantono Bintaro, Jatmiko Hardi Bintoro, Jatmiko Hardi Budha, Al Sura Tri Budi Kriswanto Budi Kriswanto, Budi Chalen Vito Nanda Didik , Pudji Restanto Didik Pudji Restanto Didik Pudji Restanto Dimas Ganda Permana Putra Distiana Wulanjari Dwi Erwin Kusbianto Farchan Mushaf Al Ramadhani Gusna Merina, Gusna Habriantono, Bakhroini Handayani, Etty Hanifah, Fairuz Luthfi Hasbi Mubarak Suud Hidayatullah, Raihan Iqbal Felayati Iqbal Felayati Iryono Laily Ilman Widuri, Laily Ilman Listya Purnamasari Mochammad Wildan Jadmiko Mohammad Candra Prayoga Mona, Muhammad Dima Say Muhammad Dima Say Mona Munandar, Denna Eriani Nada Nur Rizkiyah Nadiya, Nisma Riyadh Niagra, Risnanda Deva Martha Pamungkas, Wahyu Eka Parawita Dewanti Parawita Dewanti Parawita Dewanti Prayoga, Mohammad Candra Putri, Widya Kristyanti Rachmi Masnilah Raihan Hidayatullah Ratnasari, Tri Restanto, Didik Pudji Rusdiana, Riza Yuli Safira, Arlinda Safitri, Berliana Desta Sandy Al Firdauzi Setiawan, Moh Rizqi Sholeh Avivi Sigit Soeparjono Slameto Slameto Slameto Soeharto Suandana, Febery Hery Suhartiningsih Dwi Nurcahyanti Suharto Suharto Sukamto, Ika Sumiyarsi Sundahri, Sundahri Suud, Hasbi Mubarak Thohirin, Ahmad Nazmi Tri Agus Siswoyo Vega Kartika Sari Vito Nanda, Chalen Wagiyana Wagiyana Wagiyana, Wagiyana Wahyu Indra Duwi Fanata Wahyu Indra Duwi Fanata Widya Kristiyanti Putri Wirayudo, Haykal FIkram Wulanjari, Distiana Zahro, Fauziatuz