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All Journal HAYATI Journal of Biosciences Jurnal Natur Indonesia AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL ILMU PERTANIAN Biogenesis: Jurnal Ilmiah Biologi CEMARA Journal of Degraded and Mining Lands Management Jurnal Keteknikan Pertanian Tropis dan Biosistem Jurnal Agro Planta Tropika JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrin : Jurnal Penelitian Pertanian AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA Jurnal Agrotek Indonesia Jurnal Abdi Insani Jurnal Ilmiah Agribios BIOVALENTIA: Biological Research Journal Journal of Applied Agricultural Science and Technology Journal of Soilscape and Agriculture PAPUMA: Journal of Comunnity Services Jurnal Natur Indonesia
Khozin, Mohammad Nur
Program Studi Agronomi, Fakultas Pertanian, Universitas Jember, Jawa Timur
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences

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Journal : Jurnal Agro

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Respon kultur antera tembakau terhadap media bernutrisi dan AgNPs secara in vitro Khozin, Mohammad Nur; Ahnaf, Yusuf Dary; Dewanti, Parawita; Restanto, Didik Pudji; Iryono
Jurnal AGRO Vol. 12 No. 2 (2025)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/j.agro.49119

Abstract

Tanaman tembakau (Nicotiana tabacum L.) merupakan tanaman musiman bernilai ekonomi tinggi, namun produksinya di Indonesia terus mengalami penurunan dalam beberapa tahun terakhir. Penggunaan varietas hibrida dapat menjadi solusi karena memiliki produktivitas dan ketahanan penyakit yang lebih baik, tetapi pembentukannya secara konvensional memerlukan waktu lama. Teknik kultur antera dapat digunakan untuk mempercepat pembentukan galur murni karena menghasilkan tanaman homozigot hanya dalam satu generasi. Penelitian ini bertujuan untuk menganalisis interaksi antara jenis media kultur (MS dan Chu’s N6) dengan penambahan beberapa konsentrasi Nanopartikel silver (AgNPs) yaitu 0,0; 2,5; 5,0; 7,5; dan 10,0 mg L⁻¹ terhadap pertumbuhan antera tembakau secara in vitro. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 10 perlakuan dan 3 ulangan, kemudian data dianalisis menggunakan ANOVA dan diuji lanjut DMRT dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa media MS merupakan media terbaik dalam mempercepat dan memperbanyak induksi swelling, kalus, tunas, dan akar, sedangkan konsentrasi AgNPs terbaik diperoleh pada 2,5 mg L⁻¹ yang mampu meningkatkan respons pertumbuhan serta sedikit mengurangi tingkat kontaminasi media. Hasil penelitian ini menunjukkan bahwa kombinasi media MS dan AgNPs dosis rendah mampu meningkatkan efisiensi regenerasi antera tembakau dan berpotensi digunakan sebagai protokol standar dalam produksi galur murni secara cepat, efisien, dan steril di laboratorium kultur jaringan. ABSTRACT Tobacco (Nicotiana tabacum L.) is a seasonal crop with high economic value, but its production in Indonesia has continued to decline in recent years. The use of hybrid varieties can be a solution because they have better productivity and disease resistance, but their conventional formation requires a long time. Anther culture techniques can be used to accelerate the formation of pure lines because they produce homozygous plants in just one generation. This study aims to determine the interaction between the types of culture media (MS and Chu's N6) with the addition of several concentrations of silver nanoparticles (AgNPs), namely 0, 2.5, 5, 7.5, and 10 mg L⁻¹ on the growth of tobacco anthers in vitro. The study used a Completely Randomized Design (CRD) with 10 treatments and 3 replications, then the data were analyzed using ANOVA and further tested with DMRT at the 95% level. The results showed that MS medium was the best medium in accelerating and increasing the induction of swelling, callus, shoots, and roots, while the best concentration of AgNPs was obtained at 2.5 mg L⁻¹ which was able to increase the growth response and slightly reduce the level of media contamination. The findings of this study indicate that the combination of MS medium and low-dose AgNPs can increase the efficiency of tobacco anther regeneration and has the potential to be used as a standard protocol in the production of pure lines quickly, efficiently, and sterilely in tissue culture laboratories.
Co-Authors . Usmadi Agustin, Vira Audita Ahmad Iqbal Rananda Ahnaf, Yusuf Dary Aji, Seto Purnomo Alfarisy, Fariz Kustiawan Alfarizi, Zulfikar Ahmad Aultavia, Reza Novita Azmi, Hilmi Tsabiatul Azzahro, Firyal Nurul Haifa Bakhroini Habriantono Bintaro, Jatmiko Hardi Bintoro, Jatmiko Hardi Budha, Al Sura Tri Budi Kriswanto Budi Kriswanto, Budi Chalen Vito Nanda Didik , Pudji Restanto Didik Pudji Restanto Didik Pudji Restanto Dimas Ganda Permana Putra Distiana Wulanjari Dwi Erwin Kusbianto Farchan Mushaf Al Ramadhani Gusna Merina, Gusna Habriantono, Bakhroini Handayani, Etty Hanifah, Fairuz Luthfi Hasbi Mubarak Suud Hidayatullah, Raihan Iqbal Felayati Iqbal Felayati Iryono Laily Ilman Widuri, Laily Ilman Listya Purnamasari Mochammad Wildan Jadmiko Mohammad Candra Prayoga Mona, Muhammad Dima Say Muhammad Dima Say Mona Munandar, Denna Eriani Nada Nur Rizkiyah Nadiya, Nisma Riyadh Niagra, Risnanda Deva Martha Pamungkas, Wahyu Eka Parawita Dewanti Parawita Dewanti Parawita Dewanti Prayoga, Mohammad Candra Putri, Widya Kristyanti Rachmi Masnilah Raihan Hidayatullah Ratnasari, Tri Restanto, Didik Pudji Rusdiana, Riza Yuli Safira, Arlinda Safitri, Berliana Desta Sandy Al Firdauzi Setiawan, Moh Rizqi Sholeh Avivi Sigit Soeparjono Slameto Slameto Slameto Soeharto Suandana, Febery Hery Suhartiningsih Dwi Nurcahyanti Suharto Suharto Sukamto, Ika Sumiyarsi Sundahri, Sundahri Suud, Hasbi Mubarak Thohirin, Ahmad Nazmi Tri Agus Siswoyo Vega Kartika Sari Vito Nanda, Chalen Wagiyana Wagiyana Wagiyana, Wagiyana Wahyu Indra Duwi Fanata Wahyu Indra Duwi Fanata Widya Kristiyanti Putri Wirayudo, Haykal FIkram Wulanjari, Distiana Zahro, Fauziatuz