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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Pengolahan Hasil Perikanan Indonesia Media Veteriner Jurnal Sain Veteriner Jurnal Veteriner Jurnal Perikanan dan Kelautan Berkala Perikanan Terubuk Majalah Kedokteran Bandung Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Agricultural Science Indonesian Journal of Pharmaceutical Science and Technology agriTECH Warta IHP (Warta Industri Hasil Pertanian) INDONESIAN JOURNAL OF PHARMACY JURNAL PANGAN Agroteknika Jurnal Mutu Pangan : Indonesian Journal of Food Quality Canrea Journal: Food Technology, Nutritions, and Culinary Journal Narra J Jurnal Kedokteran Hewan Molekul: Jurnal Ilmiah Kimia Jurnal Kefarmasian Indonesia Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research)
Tutik Wresdiyati
IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Journal : agriTECH

 1 
POTENSI BIJI DAN EKSTRAK BIJI TERATAI (Nymphaea pubescens Willd) SEBAGAI PENCEGAH DIARE PADA TIKUS PERCOBAAN YANG DIINTERVENSI E.coli ENTEROPATOGENIK Yuspihana Fitrial; Made Astawan; Soewarno Soekarto; Komang Wiryawan; Tutik Wresdiyati
agriTECH Vol 32, No 3 (2012)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.475 KB) | DOI: 10.22146/agritech.9611

Abstract

The aims of this study were to observe phytochemical components contained in waterlily seeds and its ethyl acetate extract; and to observe their antibacterial activities on rats that were intervened with diarrhea-causing bacteria (Enterophatogenic E. coli K1.1, EPEC). Treatment was given on the Sprague Dawley male rats (weighing 140 ± 5 g) for 28 days. Male rats were fed a basal diet (grup control) or the same diet containing 18.7 g/100g of waterlily’s seed flour or the same diet containing 6 g/100 g of FOS or a basal diet with 17.8 mg/ml waterlily’s seeds extract (orally route) for 2,3 or 4 weeks. After 2 weeks treatment feedings, rats were orally infected with 0.3 ml of 106 CFU/ml E. coli in a week. Cecal contents were collected at the end of each time period.The biological activity of treatment was observed by observing body weight, daily feed intake, feed effi ciency, total microorganisms, total E. coli and total lactic acid bacteria from the cecum contents of rats and histological picture of the small intestine of rats. The results showed that water lily seed contain alkaloids, fl avonoids, steroids, glycosides, tannins, saponins, and triterpenoids, whereas ethyl acetate extract of water lily seed contain alkaloids, fl avonoids, tannins, glycosides, saponins and triterpenoid. Substitution treatment with waterlily seed in rations affect to total E. coli in the cecum, both after EPEC intervention and after the intervention stopped. While the provision of waterlily seed extracts affect to total E. coli in the cecum after the intervention stopped. The feed effi ciency in group substituted with waterlily seed was higher than the control and its extract (P <0.05). Substitution treatment of waterlily seed and the extract could prevent damage to the intestinal villi resulting from enterophatogenic E.coli attack. The results of this study indicated that both waterlily seed and its extract contain active compounds that could prevent and inhibit the growth of E. coli causing diarrhea in rats.ABSTRAKPenelitian ini bertujuan untuk mengetahui komponen Þ tokimia yang terdapat pada biji teratai dan ekstrak etil asetat bijiteratai dan mengetahui aktivitas antibakterinya terhadap E.coli penyebab diare (E.coli Enteropatogenik K1.1, EPECK1.1) pada tikus percobaan. Perlakuan diberikan pada tikus jantan jenis Sprague Dawley (berat 140 ±5 g) selama28 hari. Tikus percobaan dibagi menjadi 4 grup yaitu, grup 1, kontrol (mendapatkan ransum standar), grup 2 yangmendapat ransum yang disubstitusi tepung biji teratai (18,7 g/100 g), grup 3 yang mendapat ransum yang disubstitusiFOS (fruktooligosakarida, 6 g/100 g), dan grup 4 yang mendapat ransum standar dan ekstrak etil asetat biji teratai(17,8 mg/ml). Setelah 2 minggu perlakuan ransum, tikus percobaan diintervensi secara oral dengan 0,3 ml dari 106CFU/ml EPEC K1.1 selama 1 minggu sehingga diare. Aktivitas biologis ransum perlakuan diamati dengan mengamatibobot badan, konsumsi ransum per hari, eÞ siensi ransum, total mikroba, total E.coli dan total bakteri asam laktatdari isi sekum tikus percobaan. Hasil penelitian menunjukkan bahwa biji teratai mengandung alkaloid, ß avonoid,steroid, glikosida, tanin, saponin, dan triterpenoid, sedangkan ekstrak etil asetat biji teratai mengandung alkaloid,ß avonoid, tanin, glikosida, saponin dan triterpenoid. Substitusi biji teratai pada ransum mampu menurunkan totalE.coli isi sekum, baik setelah intervensi maupun setelah intervensi EPEC dihentikan. Sementara pemberian ekstrakbiji teratai mampu menurunkan total E.coli isi sekum setelah intervensi dihentikan. EÞ siensi ransum pada grup yangdisubstitusi biji teratai lebih tinggi dibandingkan kontrol dan ekstrak biji teratai (P<0.05). Perlakuan substitusi bijiteratai, dan pemberian ekstrak biji teratai dapat mencegah kerusakan pada vili usus halus akibat dari serangan E.colienteropatogenik. Berdasarkan penelitian ini, baik bjii teratai maupun ekstrak biji teratai mengandung senyawa aktifyang mampu mencegah dan menghambat pertumbuhan E.coli penyebab diare.Kata kunci: Biji teratai, Enterophatogenic Escherichia coli, ekstrak etil asetat, antibakteri, diare.
Co-Authors . Komari A.A. Ketut Agung Cahyawan W Abidah, Puri Adzrok Adi Winarto Adurrasyid, Zaid Afifah, Diana N. Akmal, Muhammad Ichlasul Alamsah Firdaus Alfarisi, Hamzah Amilia Dayatri Uray Anak Agung Istri Sri Wiadnyani Ananda Putri Cahyani Andi Early Febrinda Andi Mu’nisa Ani Karmila Ans Budi Hartanta ANZS BUDY HARTANTA Arief Boediono Armando M Saragih Aziz, Sandra Azizatul Ulfa Bambang Pontjo Priosoeryanto Bambang Purwantara Bella Dinar Fauqii Cahyani Berry Juliandi Chitisankul, Wanida T. Citra Noviana Dadang Supriatna Dadi Hidayat Maskar Dadi Hidayat Maskar, Dadi Hidayat Damayanti, Aprilia F. Damiana Rita Ekastuti Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi DEDDY MUCHTADI Diini Fithriani Dimas Ahmad Rizaldi Dwi Febiyanti - Dwi Kesuma Sari Dwi Utami, Septi Eiichiro Fukusaki Eka Prasetiawan Eka Prasetiawan Eka Prasetiawan Elmeizy Arafah Erna Suzanna Evi Damayanti Evy Damayanthi Fithriani, Diini Fitrah Asma Ulhusna Fransiska R Zakaria Hadiningtias, Primanisa Hamzah Alfarisi Hamzah Alfarisi Hamzah Alfarisi Hamzah Alfarisi Hardinsyah Hidayati, Mustika I Komang Gede Wiryawan I Nyoman Suarsana Ichsani, Nadya Inas Suci Rahmawati Indah Fajarwati, Indah Indira Saputra Irma Isnafia Arief Isnafia Arief , Irma Ita Djuwita Jefriaman Sirait Joko Hermanianto Karnila, Rahman Karnila Ketut Adnyane Mudite koekoeh santoso Komang G Wiryawan Komari Komari Komari Komari Lasmiati, Ni Nengah LUSIA YUNI HASTANTI Made Astawan MADE ASTAWAN Made Astawan Made Darawati Manalu, Johanes Marojahan MARIA BINTANG Maryani Suwarno Maryani Suwarno Maryani Suwarno Muhamad Firdaus Muhamad Firdaus Muhammad Agus Muljanto Muhammad Ichlasul Akmal Muhammad Ichsan Mursyid . Mursyid Mursyid Mustika Hidayati Nadya Ichsani Nancy Dewi Yuliana NASTITI KUSUMORINI Nelis Imanningsih Ni Nengah Lasmiati Novita, Rias R. Palupi, Nurheni Sri Prabandari, Silvia Arin Prasetyawati, Renny Candra Prayudani, Ayu P G Prima Yaumil Fajri Prima Yaumil Fajri Putri, Anisya Saeila Putri, Sastia P. Putri, Sastia Prama Putty Anggi Lestari Rafidha Irdiani Rahmawati, Siti Irma Ramdhani, Rizal Pauzan Ratnaningsih Eko S. Renny Candra Prasetyawati Ria Ceriana Rini Kesenja Rita Khairina Sadiah, Siti Saithong, Pramuan Sandra Arifin Aziz Saputra, Indira Saragih, Armando M SARASWATI SARASWATI Sarwono Waspadji Sarwono Waspadji Sarwono Waspadji Sastia Prama Putri Savitri Novelina Septi Dwi Utami Setyawati S Karyono Setyawati S. K. Setyawati S. Karyono Sidik . Silvia Arin Prabandari Siti Harnina Bintari Siti Sa&#039;diah Siti Sa'diah Siti Sa'diah Siti Sadiah Siti Sa’diah SITI SA’DIAH Soewarno S Soekarto Soewarno Soekarto Sofiawati Sofiawati Sofiawati, Sofiawati Sri Rahmatul Laila Sri Widowati Sri Widowati Srihadi Agungpriyono Subangkit, Mawar Sukarno Sukarno Suliantari . Sussi Astuti Taopik Ridwan TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TEGUH SURANTA SINULINGGA Tessa Winandita Trioso Purnawan Tryas, Anisha Ayuning Ulhusna, Fitrah Asma VENNY FEBRIYANI Vera Di Nurwati Wasmen Manalu Yana Nurdiana Yeni Setiorini Yeni Setiorini Yenni MS Nababan YOLI ZULFANEDI Yuspihana Fitrial