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Leukocyte Profile of Dexamethasone-Induced Quail After Administration of Bombyx Mori Silkworm Pupae Extract Santoso, Koekoeh; Mahendra, Arindina; Isdoni, Isdoni; Jumadin, La; Farajallah, Achmad
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v9i2.24061

Abstract

Poultry especially Japanese quails are susceptible to the environmental threat that induced glucocorticoid hormone secretion. Glucocorticoids stimulate oxidative stress and a decrease in the immune system. The purpose of this research is to study the potential of silkworm extract (Bombyx mori) to overcome oxidative stress and reduced the immune system in quails that were induced with dexamethasone as glucocorticoid. Thirty-two adult quails were used and were given dexamethasone. Those quails were divided into 4 groups, four groups were given 0 mg, 0.16 mg/kg, 0.32 mg/kg, and 0.64 mg/kg of silkworm extract for each quail. The parameters that were observed are total leucocyte count, differential leucocyte count, and heterophil/lymphocyte ratio. The results are not significant compared to the groups that were not given any supplements. This study shows that the administration of silkworm extract is not effective for recovery purposes.Keywords: antioxidant, quail, Bombyx mori, dexamethasone
Genetic Diversity and Differentiation of Sand Crab (Albunea symmysta) (Crustacea: Decapoda: Hippoidea) from Java and Papua, Indonesia Putri, Vinna Windy; Widayati, Kanthi; Wardiatno, Yusli; Farajallah, Achmad
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.671-677

Abstract

In Indonesia, the sand crab Albunea symmysta distribution includes Java, Sumatra, and Moluccas. Populations are assumed to be differentiated between western and eastern Indonesia due to the Wallace line; however, no information on the genetic diversity and differentiation of A. symmysta in Indonesia is available. This study was conducted to clarify the genetic diversity and differentiation of A. symmysta in Indonesia. During the sampling campaign, A. symmysta specimens were found only in Java and Papua. Genetic analysis based on the 643-bp fragment of the partial COI gene from 32 individuals revealed significant genetic differentiation between the Java and Papua populations (FST = 0.94, p < 0.005). The haplotype connectivity of the five A. symmysta sampling sites revealed a two-clade network. Based on analysis of molecular variance, most of the variation was found among populations (94.21%) rather than within populations (5.79%). Tajima's D and Fu's FS values indicated a population expansion. Overall, the findings support a high level of differentiation between the Java and Papua A. symmysta populations, suggesting the separation of population stocks due to low connectivity.
Genetic Structure of Gallus varius Based on Middle-Lower Section of Control Region mtDNA Achmad, Alfiyan; Farajallah, Achmad; Ulfah, Maria; Perwitasari-Farajallah, Dyah; Muladno, Muladno
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.548-558

Abstract

Gallus varius, an indigenous bird species of Indonesia, demonstrates significant genetic diversity. The genetic diversity assessment in G. varius encompassed both mitochondrial DNA (mtDNA) and nuclear DNA, with the mtDNA analysis primarily centered on domain I of the control region. This study aimed to describe the genetic diversity and structure of G. varius inhabiting Java, Madura, Lombok and Sumbawa based on the middle-lower part of control region mtDNA. Genomic DNA was extracted from the calamus tip of feather, then the middle-lower part of control region was amplified and sequenced using two pairs of primers. In the examined control region, spanning from 944 to 1,008 bp, 13 bp of nucleotide variation was observed, with nucleotide diversity at 0.0021. Between G. varius samples and the reference (NC_007238.1), a total of 7 haplotypes were identified, 5 unique and 2 shared haplotypes, with haplotype diversity at 0.7692. The substantial diversity of haplotypes in this study and two previous study suggests that the genetic diversity of G. varius has remained stable over the past two decades. Additionally, genetic distance data indicate there is multiple G. varius subspecies, and the haplotype network accentuates signs of population differentiation.
Food Habits of the Common Palm Civet (Paradoxurus hermaphroditus) in Pangandaran Nature Reserve, West Java, Indonesia: a Preliminary Report Tsuji, Yamato; Tatewaki, Takafumi; Farajallah, Achmad; Tanaka, Hiroyuki; Arum Widayati, Kanthi; Suryobroto, Bambang
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.687-692

Abstract

We conducted a preliminary survey of the wild common palm civet Paradoxurus hermaphroditus in a secondary forest in Pangandaran Nature Reserve, West Java, Indonesia. We collected 125 fresh fecal samples between October 2018 and August 2019. We conducted faecal analyses after species confirmation by camera trapping and faecal DNA analysis. Almost all faeces contained fruits and seeds (frequency of occurrence: 97.6%), while the percentage of animal matter (including mammals, birds, insects, non-insect arthropods, and molluscs) was much lower (22.4%). We observed no seasonal differences in major faecal contents. Higher dependence on fruits by the common palm civets was similar to those in other study sites. Seeds of at least eight different plant species were found in the faeces of the common palm civets, which implied that the common palm civets would play roles as seed dispersal agents.
Characterization of CDS Region of Exons 1 and 2 of SOX9 Gene as Potential Gene in Construction of Syrinx Structure in Junglefowl (Gallus sp.) Alfiyan, Achmad; Farajallah, Achmad; Maria, Ulfah; Perwitasari-Farajallah, Dyah; Muladno, Muladno
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1130-1143

Abstract

The crowing of male Gallus exhibits diverse sound patterns. This is believed to be related to the phenotypic diversity of vocal organs, one of which is influenced by the nucleotide diversity of the associated genes. The SOX9 gene, involved in cartilaginous tissue growth and development, is reported to contribute e in the development of larynx and syrinx. This study aimed to characterize the CDS regions of exons 1 and 2 of the SOX9 gene in junglefowl to assess its diversity. Genomic DNA was extracted from ten individuals of G. varius from Lombok and Sumbawa. The CDS regions of SOX9 gene exons 1 and 2 were amplified using two primer pairs. Additionally, the CDS regions of SOX9 gene exons 1 and 2 from 54 junglefowl SRA data in an online repository were mapped and analyzed. The study identified all nucleotide sequences as CDS regions of SOX9 gene exons 1 and 2. Six shared, and 24 unique haplotypes were constructed. A putative amino acid sequence common to all Gallus species was also identified. The diversity observed in the CDS regions of SOX9 gene exons 1 and 2 nucleotide sequence showed a different level with the diversity observed in its amino acid sequence.
PENGARUH SUHU TERHADAP DIFERENSIAL LEUKOSIT SERTA KADAR MALONDIALDEHIDE (MDA) BURUNG PUYUH (Coturnix coturnix Japonica) Maheshwari, Hera; Sasmita, Annissa Nuridfi; Farajallah, Achmad; Achmadi, Pudji; Santoso, Koekoeh
Bioma Vol. 13 No. 2 (2017): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (506.533 KB) | DOI: 10.21009/Bioma13(2).4

Abstract

The main problem in tropical poultry farming that often happens is a high mortality rate. Deaths occurrance mostly by heat stress, a condition which is caused by high temperature ( > 28 ° C). Prolonged heat stress was given to 24 quails grouped in four groups: A (39 ° C), B (41 ° C), C (43 ° C) and D (45 ° C). Provision of heat stress was done when the quails were 2 days old. Blood sampling was performed at the age of 6 weeks. Overall, there was an increase in the number of white blood cell, heterophile, eosinophil, monocyte, the ratio between heterophyll and lymphocyte and malondialdehyde (MDA) levels in all treatment groups. While the number of lymphocytes decreased in all treatment groups. Basophils had a significant increase in group C, but there was a decrease in group B and D decreased from control. This indicates that maintenance above temperature > 25 ° C can cause oxidative stress due to heat stress in quail.
Variations of Exon 2-3 of the Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha (BCKDHA) Gene and Its Flanking Intronic Region in Madura Cattle Febriana, Asri; Farajallah, Achmad; Wahidah, Baiq Farhatul; Perwitasari, Dyah
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13436

Abstract

Indonesia is one of a country with high biodiversity and genetic resources. One of them is Madura cattle that derived from crossing between exotic cattle, namely zebu cattle with local Indonesian cattle, namely Bali cattle. Branched-chain alpha-keto acid dehydrogenase is an enzyme complex that metabolizes branched chain amino acids, namely valine, leucine and isoleucine. This study aimed to analyze exons 2-3 variation of the BCKDHA gene and its flanking region in Madura cattle. The sequences of it were obtained using DNA sequencing techniques. A total of seven variations were found, one variation located in the flanking region adjacent to the 5' end of exon 2 of the BCKDHA gene. The A392G variation found in exon 3 does not cause any amino acid changes. There is no variation found in exon 2, it indicates that the exon regions of BCKDHA gene are more conserved than introns in the Madura cattle.
Variations of Exon 2-3 of the Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha (BCKDHA) Gene and Its Flanking Intronic Region in Madura Cattle Febriana, Asri; Farajallah, Achmad; Wahidah, Baiq Farhatul; Perwitasari, Dyah
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13436

Abstract

Indonesia is one of a country with high biodiversity and genetic resources. One of them is Madura cattle that derived from crossing between exotic cattle, namely zebu cattle with local Indonesian cattle, namely Bali cattle. Branched-chain alpha-keto acid dehydrogenase is an enzyme complex that metabolizes branched chain amino acids, namely valine, leucine and isoleucine. This study aimed to analyze exons 2-3 variation of the BCKDHA gene and its flanking region in Madura cattle. The sequences of it were obtained using DNA sequencing techniques. A total of seven variations were found, one variation located in the flanking region adjacent to the 5' end of exon 2 of the BCKDHA gene. The A392G variation found in exon 3 does not cause any amino acid changes. There is no variation found in exon 2, it indicates that the exon regions of BCKDHA gene are more conserved than introns in the Madura cattle.
DISTRIBUTION OF Charybdis (Archias) padangensis Leene & Buitendijk, 1952 (CRUSTACEA: DECAPODA: BRACHYURA) IN THE WATERS OF SUMATRA AND JAVA Hanim, Nisfa; Farajallah, Achmad; Widayat, Kanthi Arum i; Mashar, Ali
JURNAL BIOSAINS Vol. 10 No. 3 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i3.61142

Abstract

Until this decade, Charybdis (Archias) padangensis was only from Padang, West Sumatra. It was reported first and the only one in 1952. We obtained several crab samples based on the by-catch from daily fishermen in several locations. Based on morphological identification, we obtained several Charybdis (Archias) padangensis individuals from two locations in Sumatra and one location in Java. The results of this research add to data on the distribution of the species, which was previously only known from the locality type.
Polymorphism of Cyt-b Gene in Several Indonesian Cattle Using PCR-RFLP Method Agung, Paskah Partogi; Perwitasari, Dyah; Farajallah, Achmad; Said, Syahruddin; Kaiin, Ekayanti Mulyawati; Saputra, Ferdy; Hermansyah, Alfandy; Kuswati, Kuswati; Susilorini, Tri Eko; Zein, Moch. Syamsul Arifin
Buletin Peternakan Vol 48, No 2 (2024): BULETIN PETERNAKAN VOL. 48 (2) MAY 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i2.87484

Abstract

The cytochrome b (Cyt-b) gene is one of the genes that is located in the mitochondrial DNA. Variations in the Cyt-b gene can be used to compare different animal species to investigate the origin of certain animal species. This study aimed to assess the genetic diversity of Indonesian local cattle breeds, including Bali cattle as an Indonesian native cattle breed and Banteng as the wild type of Bali cattle, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A total of 336 individual DNA samples from Indonesian cattle breeds were analyzed in this study. The RFLP method using three restriction enzymes, i.e., HinfI, HaeIII, or XbaI, was used to identify the variation of the Cyt-b gene. The Cyt-b gene was polymorphic based on the PCR-RFLP method. There were six alleles of the Cyt-b gene found in this study, i.e., A and B allele (HinfI), C and D allele (HaeIII), X and Y allele (XbaI). All alleles can be found in Pasundan, Madura, and PO cattle. Pesisir cattle have the highest allele frequency D. The UPGMA results showed three clusters of Indonesian native cattle in this study. Cluster 1 consists of Pasundan, Banteng, and Bali cattle. Cluster 2 consists of Madura, PO, and SO cattle. Pesisir was separated from other cattle in cluster 3. The X allele could become an indicator to distinguish Banteng and Bali cattle.
Co-Authors A Anggraeni A Anggraeni A Anggraini A Batubara A. Anggraeni A. Anggraeni Achmad Alfiyan Achmad Alfiyan Achmad, Alfiyan AGUS A. HAKIM Agus Alim Hakim AKICHIKA MIKAMI ALFIYAN, ACHMAD Ali Mashar Ali Mashar Ali Suman AMIR HAMIDY AMIR HAMIDY Amir Hamidy Amir Hamidy Amir Hamidy ANIFA BINTAR Annawaty Annawaty, Annawaty ANTOINE FOUQUET Anzalia Eka Putri Arinana Arinana Arma Aditya Kartika Aron Batubara Aron Batubara Asri Febriana Asri Febriana, Asri AWAL RIYANTO AWAL RIYANTO B Tiesnamurti B. Tiesnamurti Baiq Farhatul Wahidah Bambang Purwantara Bambang Suryobroto Burhanuddin Burhanuddin C Sumantri C Sumantri C. SUMANTRI C. Sumantri Cece Sumantri D Perwitasari D, Nurhayati D. Herdiana D. Rahmat Daisy Wowor Daisy Wowor Daisy Wowor, Daisy Dede Setiadi DEDY D. SOLIHIN Devi Sandriliana DEWI APRI ASTUTI DEWI PURNAMA DIAH IRAWATI DWI ARINI DIKI MUHAMAD CHAIDIR DWI SENDI PRIYONO Dwi Sendi Priyono DYAH PERWITASARI Dyah Perwitasari DYAH PERWITASARI -FARAJALLAH E Andreas E. Andreas E. Andreas Eric N. Smith Eric N. Smith Erni Sulistiawati Eryk Andreas EVY ARIDA Farid Akhsani Farjun Lahiaro Fauzan, Muhammad Fakhri Ferdy Saputra H. Nuraini HANIM, NISFA Hera Maheshwari Hermansyah, Alfandy I Inounu Imas Batubara Intan Ratu Pratiwi Irma Shita Arlyza Isdoni Bustaman J F Salamena Jarmuji Jarmuji Jeane Siswitasari Mulyana Joni Setiawan Jumadin, La Jusmaldi Jusmaldi Kaiin, Ekayanti Mulyawati KANTHI ARUM WIDAYATI Khairiza Lubis khairiza lubis Koekoeh Santoso KREY KREY Kuswati Kuswati Lora purnamasari, Lora Luthfia Nuraini Rahman M Doloksaribu M Karmita M. CHANDRA WIDJAJA M. Doloksaribu Mahendra, Arindina Majariana Krisanti Maria Ulfah MAULIDA NUR ANNISA Mennofatria Boer Meutiya Agustina Meutiya Agustina, Meutiya MISBAHUL MUNIR Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin MUFTI SUDIBYO Mufti Sudibyo Muhammad Fakhri Fauzan Muhammad Khalid Yudhistiro MULYADI Mulyadi N Satriani NEVIATY PUTRI ZAMANI Nirmala Fitria Firdhausi NISFA HANIM Nisfa Hanim Nisfa Hanim Novita Anggraeni NUR RACHMAN Nurhayati D Nurlisa Alias Butet Nurulvadini Safrudin Paskah Partogi Agung Person Pesona Renta Pradana, Tengku Gilang Pristina Tuti Wijayanti Pudji Achmadi Putri, Vinna Windy R Diyono R Misrianti R R Noor R R Noor R.R. Noor R.R. Noor Rarah Ratih Adjie Maheswari REVA ADZANI NURJANNAH RIKA RAFFIUDIN Riki Andika RISKA RISMAWATI Rissa Herawati Ginting Riyanto, Awal Riyanto, Awal Rose Ida Ummah ROSE IDA UMMAH RUDI A. WAHYUDIN Rudi Alek Wahyudin Sasmita, Annissa Nuridfi Silvia Puspitasari Siti Lutvaniyah Slamet Mulyanto Smith, Eric N. Soemarno, Suprayogo Soemarno, Suprayogo Syahruddin Said Tanaka, Hiroyuki TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TARUNI SRI PRAWASTI Tatewaki, Takafumi Taufan Nurzaman Sulaeman TAUFAN NURZAMAN SULAEMAN Tazkiah Auliaputri Tri Eko Susilorini Tsuji, Yamato Tyani Fitrian Ummahatul Mujahidah Vella Nurazizah Djalil Vendi Eko Susilo WAHYU TRILAKSONO WAHYU TRILAKSONO Wahyudin Abd. Karim, Wahyudin Abd. Widayat, Kanthi Arum i Widayati, Kanthi WINDRA PRIAWANDIPUTRA, WINDRA Windra Priawandputra Yanti Ariyanti YANTI ARIYANTI Yanti Ariyanti Yudhistiro Muhammad Khalid Yusli Wardiatno