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Variations of Exon 2-3 of the Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha (BCKDHA) Gene and Its Flanking Intronic Region in Madura Cattle Febriana, Asri; Farajallah, Achmad; Wahidah, Baiq Farhatul; Perwitasari, Dyah
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13436

Abstract

Indonesia is one of a country with high biodiversity and genetic resources. One of them is Madura cattle that derived from crossing between exotic cattle, namely zebu cattle with local Indonesian cattle, namely Bali cattle. Branched-chain alpha-keto acid dehydrogenase is an enzyme complex that metabolizes branched chain amino acids, namely valine, leucine and isoleucine. This study aimed to analyze exons 2-3 variation of the BCKDHA gene and its flanking region in Madura cattle. The sequences of it were obtained using DNA sequencing techniques. A total of seven variations were found, one variation located in the flanking region adjacent to the 5' end of exon 2 of the BCKDHA gene. The A392G variation found in exon 3 does not cause any amino acid changes. There is no variation found in exon 2, it indicates that the exon regions of BCKDHA gene are more conserved than introns in the Madura cattle.
Variations of Exon 2-3 of the Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha (BCKDHA) Gene and Its Flanking Intronic Region in Madura Cattle Febriana, Asri; Farajallah, Achmad; Wahidah, Baiq Farhatul; Perwitasari, Dyah
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13436

Abstract

Indonesia is one of a country with high biodiversity and genetic resources. One of them is Madura cattle that derived from crossing between exotic cattle, namely zebu cattle with local Indonesian cattle, namely Bali cattle. Branched-chain alpha-keto acid dehydrogenase is an enzyme complex that metabolizes branched chain amino acids, namely valine, leucine and isoleucine. This study aimed to analyze exons 2-3 variation of the BCKDHA gene and its flanking region in Madura cattle. The sequences of it were obtained using DNA sequencing techniques. A total of seven variations were found, one variation located in the flanking region adjacent to the 5' end of exon 2 of the BCKDHA gene. The A392G variation found in exon 3 does not cause any amino acid changes. There is no variation found in exon 2, it indicates that the exon regions of BCKDHA gene are more conserved than introns in the Madura cattle.
DISTRIBUTION OF Charybdis (Archias) padangensis Leene & Buitendijk, 1952 (CRUSTACEA: DECAPODA: BRACHYURA) IN THE WATERS OF SUMATRA AND JAVA Hanim, Nisfa; Farajallah, Achmad; Widayat, Kanthi Arum i; Mashar, Ali
JURNAL BIOSAINS Vol. 10 No. 3 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i3.61142

Abstract

Until this decade, Charybdis (Archias) padangensis was only from Padang, West Sumatra. It was reported first and the only one in 1952. We obtained several crab samples based on the by-catch from daily fishermen in several locations. Based on morphological identification, we obtained several Charybdis (Archias) padangensis individuals from two locations in Sumatra and one location in Java. The results of this research add to data on the distribution of the species, which was previously only known from the locality type.
Polymorphism of Cyt-b Gene in Several Indonesian Cattle Using PCR-RFLP Method Agung, Paskah Partogi; Perwitasari, Dyah; Farajallah, Achmad; Said, Syahruddin; Kaiin, Ekayanti Mulyawati; Saputra, Ferdy; Hermansyah, Alfandy; Kuswati, Kuswati; Susilorini, Tri Eko; Zein, Moch. Syamsul Arifin
Buletin Peternakan Vol 48, No 2 (2024): BULETIN PETERNAKAN VOL. 48 (2) MAY 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i2.87484

Abstract

The cytochrome b (Cyt-b) gene is one of the genes that is located in the mitochondrial DNA. Variations in the Cyt-b gene can be used to compare different animal species to investigate the origin of certain animal species. This study aimed to assess the genetic diversity of Indonesian local cattle breeds, including Bali cattle as an Indonesian native cattle breed and Banteng as the wild type of Bali cattle, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A total of 336 individual DNA samples from Indonesian cattle breeds were analyzed in this study. The RFLP method using three restriction enzymes, i.e., HinfI, HaeIII, or XbaI, was used to identify the variation of the Cyt-b gene. The Cyt-b gene was polymorphic based on the PCR-RFLP method. There were six alleles of the Cyt-b gene found in this study, i.e., A and B allele (HinfI), C and D allele (HaeIII), X and Y allele (XbaI). All alleles can be found in Pasundan, Madura, and PO cattle. Pesisir cattle have the highest allele frequency D. The UPGMA results showed three clusters of Indonesian native cattle in this study. Cluster 1 consists of Pasundan, Banteng, and Bali cattle. Cluster 2 consists of Madura, PO, and SO cattle. Pesisir was separated from other cattle in cluster 3. The X allele could become an indicator to distinguish Banteng and Bali cattle.
Testing Reliability of Serum Samples as a DNA Source on Captive Breeding Long-tailed Macaques (Macaca fascicularis) Perwitasari-Farajallah, Dyah; Farajallah, Achmad
BIOTROPIA Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.976 KB) | DOI: 10.11598/btb.2014.21.2.306

Abstract

The serum realibility was tested in captive breeding conditions on the long-tailed macaques (Macaca fascicularis). Serum and buffy coat were applied to standard protocol of DNA extration following the amplification of microsatellite DNA locus of D2S1777 by PCR and visualized PCR products by means of PAGE with silver staining.  The amount of genomic DNA extracted from serum was sufficient for genotyping individuals at DNA microsatellite locus of D2S1777 with the allele size is approximately 160 bp.  We show that serum can provide a reliable alternative for obtaining DNA where taking blood using anticoagulant is impracticable
Co-Authors A Anggraeni A Anggraeni A Anggraini A Batubara A. Anggraeni A. Anggraeni Achmad, Alfiyan Agus Alim Hakim AKICHIKA MIKAMI ALFIYAN, ACHMAD Ali Mashar Ali Suman AMIR HAMIDY Amir Hamidy Amir Hamidy Annawaty Annawaty, Annawaty Arma Aditya Kartika Aron Batubara Aron Batubara Arum Widayati, Kanthi Asri Febriana Asri Febriana, Asri B Tiesnamurti B. Tiesnamurti Baiq Farhatul Wahidah Bambang Purwantara Bambang Suryobroto Burhanuddin Burhanuddin C Sumantri C Sumantri C. Sumantri C. SUMANTRI Cece Sumantri D Perwitasari D, Nurhayati D. Herdiana D. Rahmat Daisy Wowor Daisy Wowor Daisy Wowor, Daisy Dede Setiadi Devi Sandriliana DEWI APRI ASTUTI DEWI PURNAMA Dwi Sendi Priyono Dyah Perwitasari DYAH PERWITASARI -FARAJALLAH E Andreas E. Andreas E. Andreas Eric N. Smith Eric N. Smith Erni Sulistiawati Eryk Andreas Farid Akhsani Fauzan, Muhammad Fakhri Ferdy Saputra H. Nuraini HANIM, NISFA HERA MAHESHWARI Hermansyah, Alfandy I Inounu Imas Batubara Irma Shita Arlyza Isdoni Bustaman J F Salamena Jarmuji Jarmuji Jeane Siswitasari Mulyana Joni Setiawan Jumadin, La Kaiin, Ekayanti Mulyawati KANTHI ARUM WIDAYATI Khairiza Lubis koekoeh santoso KREY KREY Kuswati Kuswati Lora purnamasari, Lora Luthfia Nuraini Rahman M Doloksaribu M Karmita M. Doloksaribu Mahendra, Arindina Maria Ulfah Maria Ulfah Mennofatria Boer Meutiya Agustina Meutiya Agustina, Meutiya Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin Mufti Sudibyo N Satriani NEVIATY PUTRI ZAMANI Nirmala Fitria Firdhausi Nisfa Hanim Nisfa Hanim Novita Anggraeni Nurhayati D Nurlisa Alias Butet Nurulvadini Safrudin Paskah Partogi Agung Person Pesona Renta Pradana, Tengku Gilang Pudji Achmadi Putri, Vinna Windy R Diyono R Misrianti R R Noor R R Noor R.R. Noor R.R. Noor Rarah Ratih Adjie Maheswari RIKA RAFFIUDIN Rissa Herawati Ginting Riyanto, Awal Riyanto, Awal Rose Ida Ummah Rudi Alek Wahyudin Sasmita, Annissa Nuridfi Silvia Puspitasari Siti Lutvaniyah Slamet Mulyanto Smith, Eric N. Soemarno, Suprayogo Soemarno, Suprayogo Syahruddin Said Tanaka, Hiroyuki TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TARUNI SRI PRAWASTI Tatewaki, Takafumi Tri Eko Susilorini Tsuji, Yamato Vella Nurazizah Djalil Wahyudin Abd. Karim Widayat, Kanthi Arum i Widayati, Kanthi Wijayanti, Pristina Tuti Yanti Ariyanti Yanti Ariyanti Yusli Wardiatno