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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis Buletin Kimia VALENSI CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) JURNAL KIMIA SAINS DAN APLIKASI Microbiology Indonesia Current Biochemistry The Journal of Pure and Applied Chemistry Research Jurnal Riset Kimia Indonesian Journal of Pharmaceutical Science and Technology Jurnal Sumberdaya Hayati SC (Savana Cendana) : Jurnal Pertanian Konservasi Lahan Kering BERITA BIOLOGI BIOMA Indonesian Journal of Chemistry EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Menara Perkebunan Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia Pharma Xplore : Jurnal Sains dan Ilmu Farmasi Indonesian Journal of Applied Research (IJAR) Jurnal Pusat Inovasi Masyarakat Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Indonesian Journal of Jamu
LAKSMI AMBARSARI
Departemen Biokimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, IPB University Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Journal : Current Biochemistry

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Bioethanol Production by Using Detoxified Sugarcane Bagasse Hydrolysate and Adapted Culture of Candida tropicalis Inda Setyawati; Laksmi Ambarsari; Siti Nur'aeni; Suryani Suryani; Puspa Julistia Puspita; Popi Asri Kurniatin; Waras Nurcholis
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

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Abstract

Ethanol is considered as the most promising alternative fuel, since it can be produced from a variety of agriculturally-based renewable materials, such as sugarcane bagasse. Lignocellulose as a major component of sugarcane bagasse is considered as an attractive renewable resource for ethanol production due to its great availability and relatively low cost. The major problem of lignocellulose is caused by its need for treatment to be hydrolyzed to simple sugar before being used for bioethanol production. However, pretreatment using acid as hydrolyzing agent creates some inhibitor compounds that reduce ethanol production because these compounds are potential fermentation inhibitors and affect the growth rate of the yeast. Reduction of these by-products requires a conditioning (detoxification and culture starter adaptation). Thus, the aim of this study was to evaluate bioethanol production by fermentation with and without detoxified sugarcane bagasse acid hydrolysate using adapted and non-adapted culture of C. tropicalis. According to this study, the highest ethanol amount was obtained about 0.43 % (v/v) with an ethanol yield of 2.51 % and theoretical yield of 4.92 % by fermentation of sugarcane bagasse hydrolysate with detoxification using the adapted strain of C. tropicalis at 72 hours fermentation time. Furthermore, the addition of 3 % glucose as co-substrate on detoxified-hydrolysate media only achieved the highest ethanol concentration 0.21 % after 24 hours fermentation with the ethanol yield 0.69 % and theoretical ethanol yield 1.35 %, thus it can be concluded that the addition of glucose could not increase the ethanol production.
The Addition Effects of Glucose as a Co-substrate on Xylitol Production by Candida guilliermondii Laksmi Ambarsari; Suryani Suryani; Steffanus Gozales; Puspa Julistia Puspita
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

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High cost production is one of the constraints of the commercial xylitol production due to high energy needed and pure raw materials. Therefore, it is necessary to improve the xylitol production eficiently with lower production cost by using microorganisms. The research objectives were to determine the optimum xylitol production from xylose by metabolism of C. guilliermondii and effect of glucose as a co-substrate in fermentation medium. The ratio of glucose : xylose (g/L) was 1:25, 1:12, 1:5 and 1:2.5 respectively. The xylitol concentration was measured by spectrophotometer method (D-sorbytol/D-xylitol kit). The result showed that the exponential phase of Candida guilliermondii was 12 h to 36 of incubation and optimum of incubation time to produce the highest xylitol was 72 h. The best ratio- of glucose : xylose to produce xylitol was 9 g/L glucose : 45 g/L xylose (1 : 5). The xylitol concentration produced from medium with the addition of glucose was 2.85 g/L. This concentration increased five times compared to that in the medium without addition of glucose that only reached 2.85 g/L. According to this study, the addition of glucose as a co-substrate could increase the xylitol production.
Anti-inflammatory Activity of Temulawak Nanocurcuminoid Coated with Palmitic Acid in The Sprague Dawley Rat Rini Novita; Laksmi Ambarsari; Syamsul Falah; Popi Asri Kurniatin; Waras Nurcholis; Latifah K Darusman
Current Biochemistry Vol. 2 No. 2 (2015)
Publisher : IPB University

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Temulawak or Curcuma xanthorrhiza Roxb, is usually used as traditional medicine (herbal medicine) that has antioxidant, anticancer, antihyperglycemic, anti-inflammatory, hepatoprotective and gastroprotective properties. The main components contained in temulawak responsible for its efficacy as a medicine are xantorhizol and curcuminoid. Curcuminoid has drawbacks, which are difficult to absorb and very quickly metabolized by the body, so that limit its bioavailability. The use of solid lipid nanoparticle carrier system (SLN) in form of palmitic acid, is known to improve the bioavailability of curcuminoid. This study aims to find the effective dose of nanocurcuminoid coated with palmitic acid that can be used as an anti-inflammatory agent. The methods used in this study, include the production of nanocurcuminoid with homogenization and ultrasonication methods, determination of particle size, polydispersity index, entrapment efficiency and anti-inflammatory activity test through rat feet edema. Nanocurcuminoid obtained in this study was 561.53 nm in size, with polydispersity index 0.309 and concentrations of curcuminoid absorbed and entrapment efficiency were 0.61±0.031 mg/mL, 58.93±3.021%, respectively. Anti-inflammatory activity of nanocurcuminoid through treated Sprague Dawley rats, showed that there were no significant difference compared with the positive control, curcuminoid extracts and empty nanoparticle. These results indicate that nanocurcuminoid with 175, 200 and 250 mg/kg.bw in doses, has greater anti-inflammatory activity (31.70%) compared to the other treatments.
Molecular Interaction Analysis of COX-2 against Curcuminoid and Xanthorizol Ligand as Anti Breast Cancer using Molecular Docking Ridho Pratama; Laksmi Ambarsari; Tony Ibnu Sumaryada
Current Biochemistry Vol. 2 No. 3 (2015)
Publisher : IPB University

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Abstract

Breast cancer is one of the most common cancer in the world that occurs in women. Thedevelopment process of cancer is regulated by a variety of pathways that involve various enzymes. COX-2 is an enzyme involved in the inflammatory process in further stages that will play importantroles in breast cancer cells progression. The uses of natural compound from plants give new hope forbreast cancer treatment with minimal side effects. Temulawak is a potential breast cancer drugs because it contains curcuminoid and xanthorizol. Curcumin and xanthorizol has been reported to have chemopreventive effect on colon cancer development. The drug that has same functions of these compounds needed to be examined with various approaches. One of the approach used in this research is molecular docking. Based on ligand analysis with Lipinski and toxicity test using ADMET, curcuminoid and xanthorizol met criteria as medicinal compounds. Curcumin had the highest binding affinity(?G) with the value -9.3 kcal/mol but still under commercial drug celecoxib binding affinity (?G) = -12,5. There were three hydrogen bonds in amino acid Arg106 and Tyr341 His75 which were amino acids in the active side of COX-2. There were 15 amino acids that have similar ties with commercial drug celecoxib. Based on the binding affinity and binding similiarity, curcuminoid and xanthorizol were predicted as compounds that have potential as competitive inhibitor of COX-2 enzyme.
Simulasi Docking Senyawa Kurkumin dan Analognya Sebagai Inhibitor Reseptor Androgen pada Kanker Prostat Arwansyah Arwansyah; Laksmi Ambarsari; Tony I Sumaryada
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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Curcumin, the major compound of Curcuma longa L, has been proven to have the toxicity effect on prostate cancer cell. This research was aimed to study the affinity and interaction of curcumin and its analogs as compettitive inhibitor to androgen hormon before working in vitro/in vivo research. Curcumin and its analogs were transformed into 3D structure, then docked to androgen receptor (3B67). The data of Gibbs energy (?G) value showed stability interaction between ligand and androgen receptor residues. The docking results showed that curcumin and its analogs have potential as inhibitor on androgen receptor. Based on results ?G score, analog 4 (1,7-bis-(3,4-dihydroxy-phenyl)-hepta-1,6-diene-3,5-dione) has highest potential as the inhibitor for androgen receptor.
Optimization of Formula Film based on Amylopectin Cassava Starch and Carrageenan as a Raw Materials of Capsule Shell G Jeni christi A; Laksmi Ambarsari; Heri Purwoto
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

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Abstract

Capsules are very important in the packaging of pharmaceutical preparations. Commercial capsule shell is generally made of gelatin from cows and pigs. Alternatives to gelatin from non-animal raw materials can be obtained from polysaccharides like starch and carrageenan. The purpose of this study was to obtain the optimum formula between amylopectin and carrageenan as a raw material subtitute for gelatin capsule shell. Program Design Expert 7.0.0 (trial version) with Response Surface Methodology (RSM) Central Composite Design was used to optimize formula with three variable factors and three response variables. Based on the analysis by determining the adjusted range, program recommends 29 optimization solution with desirability value 1. Formula 6 and 28 was selected for validation with factors 1,01% of amylopectin, 1.01% of carrageenan, 2.17% of glycerin (formula 6) and 3.00% of amylopectin, 2.00% of carrageenan, 2.90% of glycerin (formula 28). Prediction response value was 12.94% of moisture content, 6.35% of ash content (formula 6) and 12.99% of moisture content, 8.67% of ash content (formula 28). Validation result value was 21.45% of moisture content, 7.58% of ash content, 6.12 minutes of solubility in water (formula 6) and 17.67% of moisture content, 7.78% of ash content, 9.30 minutes of solubility in water.
Characterization and Toxicity of Temulawak Curcuminoid Nanoparticles Riki Riki; Popi Asri Kurniatin; Laksmi Ambarsari; waras Nurcholis; Latifah K Darusman
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

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Abstract

Temulawak exctract contains curcuminoids which have anticancer potential. However, clinical application of curcuminoid has been limited due to its low bioavailability. One of the efforts that can be developed to solve this problem is incorporated curcuminoids into Solid Lipid Nanoparticles (SLN) carriers system. The objective of this study was to characterize dan evaluate anticancer potential of temulawak ethanolic fraction nanoparticles. HPLC method was used to determined curcuminoids content of temulawak ethanolic fraction. Characterization indicators like polydispersity index, particle size, morpholgy, and entrapment efficiency. HPLC chromatogram has shown of curcumin, demethoxycurcumin, and bisdemethoxycurcumin were found in temulawak ethanolic fraction. The particle size of nanoparticles obtained in this study was 648.4 ± 95 nm with polydispersity index value of 0.216. A uniform size distribution of nanoparticles as observed by Transmission Electron Microscopy (TEM). The entrapment efficiency of curcuminoid in nanoparticles was about 29.8%. Based on results of BSLT obtained temulawak extract Lethal Concentration (LC50) value of 213.24 ppm and 828.78 ppm of nanoparticles.
Antihyperglicemic Activity of Curcuma Xanthorrizha Roxb. Nanocurcuminoid Emulsion on Streptozotocin Induced Sprague-Dawley Rat Irma Rahmayani; Laksmi Ambarsari; Mega Safithri
Current Biochemistry Vol. 3 No. 2 (2016)
Publisher : IPB University

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Abstract

Curcuminoid, a natural compound isolated from Curcuma xanthorrhiza RoxB (Temulawak) has been reported to possess antioxidant, anti-inflammatory, anticancer, antimicrobial and anti-hyperglicemic properties. The curcuminoid is weakly soluble in water that restricts its bioavailability. This problem could be overcome by incorporating curcuminoid into solid lipid nanoparticles (SLN). Nanocurcuminoid was prepare by using homogenization-ultra sonication methods. Nanocurcuminoid obtained in this study was 523.5 nm in size and polydispersity index of 0.218 with entrapment efficiency of 24.2 %. Rats were made diabetic by induction of 50 mg / kg BW STZ, treated with the chosen formula (doses 5, 10, 20 mg/kg BW) orally for 15 days. Body weight and blood glucose levels were measured from day 0, 4th,7th,11th and 15th. The results showed that 15 days of daily treatment of 10 mg/kg BW nanocurcuminoid emulsion led to a reduction of blood glucose level by 30.93±14.90 % and body weight by 15.5±13.92 %. Curcuminoid formulated in solid lipid nanoparticles could suppress a decrease in body weight and lower blood glucose levels of rats.
Uji Fitokimia dan Identifikasi Senyawa-Senyawa Metabolit Sekunder Ekstrak Etil Asetat, Etanol dan Air Tanaman Zingiber zerumbet Asal Pulau Timor Origenes Boy Kapitan; Laksmi Ambarsari; Syamsul Falah
Current Biochemistry Vol. 3 No. 3 (2016)
Publisher : IPB University

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Development of Trichodermin Nanoemulsion Based on Medium Chain Triglycerides as Antifungal of Ganoderma boninense in vitro Muhammad Alwin Azhari; Ike Wahyuni Putri; Ahmad Irvan Pratama; Radika Evita Hidayah; Laksmi Ambarsari
Current Biochemistry Vol. 4 No. 2 (2017)
Publisher : IPB University

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Abstract

Trichodermin has antifungal activity against Ganoderma boninense, but it is volatile and insoluble in water. Research on trichodermin as antifungal G.boninense has been done, but not yet at the stage of altering the nature of the compound. This study aims to develop trichodermin as antifungal G. boninense in the form of nanoenkapsulat with observed aspects including formulation, encapsulation, characterization, and antifungal activity. Trichodermin is extracted from Trichoderma harzianum by maceration. Nanoemulsion is made using Emulsion Inversion Point (EIP) method and encapsulation is done by spray drying. Antifungal activity was tested using a modified dual culture assay method. The results showed the rendement of trichodermin extraction of 0.70%. The best nanoemulsion formula is nanoemulsion with Tween 80 concentrations of 75% of the total organic phase. The formula nanoemulsi has a size of 231.95 nm, polydispersity index of 0.348, and zeta potential of -35.6 mV. Encapsulation has a yield of 5.79%. The encapsulated powder has a moisture content of 5.79%, contains a typical functional group of trichodermin compounds, and is round with an uneven surface. The antioxidant activity of nano-trichodermin encapsulation is higher than trichoderminextract and positive control (hexaconazole) with PIRG value of 84.40%.
Co-Authors -, Suryani . SURYANI A.E. Zainal Hasan Ade Heri Mulyati Adrian Adiva Adriansyah Nanda Putra Agnia Nurul Jannati Agung Isnanto Agustina L.N. Aminin Ahmad Irvan Pratama Ainia Hanifitri Aisyah Girindra Akbar, Nadhira Fathiaz Akhiruddin Maddu Akhmad Endang Zainal Hasan Alifia Mutiara Annisa Alimah, Shobiroh Nuur ANINDA INDRIANI Anja Meryandini Annisa Dhiya Athiyyah Khanza Aqilah, Rifany Fairuz Aris Tri Wahyudi Arwansyah Arwansyah Aryani, Hanifah Asrul Muhamad Fuad, Asrul Muhamad Assifah Eryandini Azhari, Muhammad Alwin Azzara Putri Elvaza Chelsea Chelsea, Chelsea Chelsy Narita Choirunnisa Miftahul Jannah Deki Geraldi Dewi Seswita Zilda Diana Widiastuti DIMAS ANDRIANTO Djarot Sasongko Hami Seno DWI NINGSIH SUSILOWATI DWI NINGSIH SUSILOWATI Dwi SUBIYARTI Dwiningsih Susilowati Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Efi Sanfitri Harahap Faliha Arinda Lestari Farhan Azhwin Maulana Fathin, Muhammad Faris Fatriani, Rizka Febrianti, Riska G Jeni christi A Genta Asvarhoza GIA PERMASKU Giovanny, Lisa Gustini Syahbirin H. M. Mochtar Hanhan Dianhar Harahap, Efi Sanfitri HARTUTIK EKA SUSANTI Hasim - Hayati Minarsih Heri Purwoto Hidayat, Radika Evita Husnawati Husnawati Husnawati, . I MADE ARTIKA I Made Samudera I Made Samudera, I Made I MADE SAMUDRA Ifa Manzila Ifa Manzila Ika Yuni Astutik Ika Yuni Astutik, Ika Yuni Ike Wahyuni Putri Ilham Kurniawan Ilham Kurniawan Imelia Dewi Imron RIYADI Inayah, Mazidah Noer Inda Setyawati, Inda Irma Rahmayani Isnanto, Agung Jaya Hardi Kapitan, Origenes Boy Karichsa Hariana Latifah K Darusman Lestari, Faliha Arinda Liliyani , Ni Putu Peggy Lisa Giovanny Made Suhandana Madyastuti, Rini Marfira, Nurul Mega Safithri Miantika, Shafillah Muhamad Rifai Muhamad Rifai Muhammad Alwin Azhari Muhammad Faris Fathin Muhammad Halim Mustika Luthfia Mustika Permatasari Mustopa, Syahrul Nabila Nur Nafiati NISA RACHMANIA MUBARIK Novia Winarti Nur Qadri Rasyid Nurhidayat Nurhidayat Nurul Marfira Nuur’Alimah, Shobiroh Okta Vino Pratama, Ahmad Irvan Purwantiningsih Sugita Puspa Julistia Puspita Putri, Ike Wahyuni Radika Evita Hidayah Rahayu, Dyah Utami Cahyaning Rasyid, Nur Qadri Ridho Pratama Rifany Fairuz Aqilah Riki Riki Riksa Nur Wahyuni Rini Kurniasih, Rini Rini Novita Riska Febrianti Rury Eryna Putri Sanro Tachibana Sarmila Sarmila Setyanto Tri Wahyudi Shobiroh Nuur' Alimah Shobiroh Nuur'Alimah Siti Nur'aeni Siti Nurjanah Siti Warnasih Soekarno Mismana Putra Steffanus Gozales Suryani - Suryani Suryani Syamsul Falah TATI NURHAYATI Tirta Setiawan Tony Sumaryada Trah Yudha Tri Puji Priyatno Tri Puji Priyatno Uswatun Hasanah Wahyuni, Riksa Nur Wanda Hamidah Waras Nurcholis Yadi Suryadi Yadi Suryadi Zahra Silmi Muscifa Zuniar Subastian