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Efektivitas Ekstrak Daun Komak (Lablab purpureus) Terhadap Sistem Imun Ikan Nila (Oreochromis niloticus) Yang Diinjeksi Bakteri Aeromonas hydrophila Rabi’atussamiah; Azhar, Fariq; Abidin, Zaenal; setyowati, Dewi Nur’aeni
Jurnal Media Akuakultur Indonesia Vol 4 No 4 (2024): Indonesian Journal of Aquaculture Medium
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/mediaakuakultur.v4i4.6140

Abstract

This study aims to determine the effect of comma leaf extract on the immune system of tilapia injected with A. hydrophila bacteria. This research was conducted for 60 days at the Fish Production and Reproduction Laboratory and Aquaculture Fish Health Laboratory, Aquaculture Study Program, Department of Fisheries and Marine Sciences, Faculty of Agriculture, University of Mataram. The method used was a completely randomized design (CRD) experiment with five treatments and three replications, namely where K- was not given comma leaf extract and was injected with 0.9% NaCl, K+ was not given comma leaf extract and was injected with A. Hydrophila bacteria, P1 (0.5%) extract comma leaf and injected with A. hydrophila bacteria, P2 (1%) of komak leaf extract and injected with A. hydophila bacteria, P3 (2%) of komak leaf extract and injected with A. hydrophila bacteria. The observed parameters were total erythrocytes, total leukocytes, hemoglobin, hematocrit, differential leukocytes, phagocytosis activity, total bacteria and survival (SR). The results showed that the average value of total erythrocytes was 1.22-1.82 x106 cells/mm3, total leukocytes were 2.08-2.8 x104 cells/mm3, hemoglobin was 3.4-5.5%, hematocrit was 14.1-17.3%, differential leukocytes consisted of 4 types of cells, namely lymphocytes. , monocytes, neutrophils and platelets. Lymphocytes 60.7-72.3%, monocytes 4.3-7.3%, neutrophils 17-24.3%, and platelets 6.3-7.7%, phagocytosis activity 44.8-66.3%, total bacteria (TB) OD620 3.39-3.84, survival (SR) 46.7-66.7 %. The addition of 2% of comma leaf extract to the feed can improve the immune system of tilapia injected with A. hydrophila bacteria
Growth of Sea Grapes (Caulerpa lentillifera) in Laboratory-Scale Cultivation With Urea As A Single Nitrogen Nutrient Eviana, Maulani; Mukhlis, Alis; Azhar, Fariq
Journal of Fish Health Vol. 4 No. 4 (2024): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v4i4.5956

Abstract

Sea grape (Caulerpa lentillifera) is a strategic green macroalgae with significant potential in aquaculture and functional food industries. However, its cultivation remains constrained by limited production techniques. This study investigated the influence of nitrogen concentration on C. lentillifera growth in a laboratory-scale cultivation system. A completely randomized experimental design was implemented with four treatments: a control group (0 ppm) and nitrogen supplementation levels of 5, 10, and 15 ppm, each replicated thrice over 21 days. Results demonstrated a significant positive correlation between nitrogen concentration and growth parameters. The 15 ppm nitrogen treatment exhibited the highest absolute growth (3.37 g), relative growth rate (13.50%), and specific growth rate (1.82% per day). Interestingly, no statistically significant difference was observed between 10 and 15 ppm treatments, suggesting a potential nitrogen uptake saturation point. Water quality parameters remained stable throughout the experiment, with temperature ranging from 28-30°C, salinity at 33-34 ppt, dissolved oxygen between 5.9-6.7 ppm, and pH maintained at 7.5-7.8. The study provides crucial insights into nitrogen's role in C. lentillifera cultivation, offering a scientific foundation for developing more efficient and sustainable macroalgae production strategies in aquaculture and functional food industries.
Efficiency of Using Kappa-Carrageenan as an Immunostimulant Agent in the Non-Specific Immune System of Vannamei (Litopenaeus vannamei) Infected with AHPND (Acute Hepatopancreatic Necrosis Disease) Azhar, Fariq; Scabra, Andre Rachmat; Muahiddah, Nuri; Affandi, Rangga Idris
Journal of Fish Health Vol. 5 No. 1 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i1.6358

Abstract

Vibrio parahaemolyticus is one of the bacteria that can attack whiteleg shrimp and cause Acute Hepatopancreatic Necrosis Disease (AHPND) or Early Mortality Syndrome (EMS) in shrimp. One alternative that can be used is the use of seaweed-based immunostimulants, namely k-carrageenan. The purpose of this study was to evaluate the effectiveness of k-carrageenan mixed in feed with different doses on increasing the immune system of whiteleg shrimp infected with AHPND. This study was conducted for 60 days with an experimental method in the form of a Completely Randomized Design (CRD) consisting of 5 treatments and 3 replications, namely, P1 (Positive Control): Commercial Feed + Vibrio parahaemolyticus Infection; P2 (Negative Control): Commercial Feed + 0.9% NaCl Infection; P3: Commercial Feed + Kappa-carrageenan 8 g / kg + Vibrio parahaemolyticus Infection; P4: Commercial Feed + Kappa-carrageenan 10 g/kg + Vibrio parahaemolyticus Infection; P5: Commercial Feed + Kappa-carrageenan 12 g/kg + Vibrio parahaemolyticus Infection. The results of this study indicate that the addition of 12 g/kg of k-carrageenan to the feed affects the survival rate and immune system of whiteleg shrimp infected with Vibrio parahaemolyticus.
Hematological Parameters and Growth Performance of Nile Tilapia (Oreochromis niloticus) Fed Fermented Papaya (Carica papaya L.) Leaf Extract Lestari, Widia; Azhar, Fariq; Diamahesa, Wastu Ayu
Journal of Fish Health Vol. 5 No. 4 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i4.8335

Abstract

Nile tilapia (Oreochromis niloticus) is one of the most important aquaculture species worldwide, but intensive farming has increased susceptibility to bacterial infections, particularly Aeromonas hydrophila, which threatens productivity and survival. This study evaluated fermented papaya (Carica papaya L.) leaf extract (FPLE) as a natural immunostimulant and sustainable alternative to antibiotics in Nile tilapia culture. A 45-day feeding trial was conducted using a completely randomized design with five treatments (0, 16, 18, and 20 mL FPLE/kg feed) and three replicates. Hematological, immunological, growth, and bacterial resistance parameters were analyzed. Results showed that supplementation with 20 mL/kg FPLE significantly increased post-infection leukocyte counts (2.82×10⁴ vs. 2.40×10⁴ cells/mm³), phagocytic activity (46.26% vs. 39.51%), and erythrocyte levels (5.85×10⁶ vs. 5.25×10⁶ cells/mm³). Differential leukocyte analysis revealed lymphocyte dominance (90.33%) and elevated monocytes (13.33%), indicating enhanced adaptive and nonspecific immunity. In addition, total bacterial load decreased by 22.4%, and specific growth rate improved (4.34% vs. 4.22%/day), while water quality remained within optimal ranges. The synergistic effects of papaya-derived bioactive compounds and Lactobacillus casei probiotics from Yakult likely contributed to immune enhancement and nutrient absorption. FPLE supplementation at 20 mL/kg optimized immunostimulation without compromising survival (77–84%), demonstrating its dual role in disease prevention and growth promotion. These findings highlight FPLE as a safe, eco-friendly feed additive with strong potential for reducing antibiotic dependence and supporting sustainable tilapia aquaculture.
Feed production techniques using local raw materials for mud crab farmers in Madak Belek Hamlet, Sekotong District Diamahesa, Wastu Ayu; Lumbessy, Salnida Yuniarti; Azhar, Fariq; Abidin, Zaenal; Badrun, Badrun; Affandi, Rangga Idris; Sumsanto, Muhammad; Diniariwisan, Damai; Rahmadani, Thoy Batun Citra; Setyono, Bagus Dwi Hari
Penamas: Journal of Community Service Vol. 5 No. 4 (2025): Penamas: Journal of Community Service
Publisher : Nur Science Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53088/penamas.v5i4.2284

Abstract

This community service activity focused on developing feed production techniques using local raw materials for mud crab farmers in Madak Belek Hamlet, Sekotong District. The main challenge faced by farmers is the high cost of commercial feed, which reduces profit margins. In fact, the area is rich in underutilized nutrient-rich local resources such as trash fish and seafood processing by-products. A participatory approach was applied, including observation, socialization, demonstration, hands-on training, and evaluation. The training emphasized feed formulation and the application of 5% wheat gluten as a binder, which had been proven in preliminary studies to enhance feed stability in water. The results showed an improvement in participants’ knowledge and skills in processing local ingredients into quality feed. The expected impacts include reducing dependence on commercial feed, lowering production costs, and improving the efficiency and sustainability of mud crab farming. For further optimization, feed performance testing on crab growth and economic feasibility analysis are required.
IMPROVING THE HEALTH OF VANNAMEI SHRIMP (Penaeus Vanname) MAINTAINED IN LOW-SALINITY MEDIA WITH POTASSIUM MINERAL SUPPLEMENTATION Scabra, Andre Rachmat; Azhar, Fariq; Affandi, Rangga Idris; Iskandar, M Sopiyan
Jurnal Perikanan Unram Vol 15 No 6 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i6.1056

Abstract

Penambahan berbagai jenis mineral pada kegiatan budidaya udang vannamei media air tawar diketahui menghasilkan pertumbuhan udang vannamei yang lebih tinggi. Namun demikian, pertumbuhan udang vannamei pada habitat aslinya, yaitu air laut, masih menghasilkan pertumbuhan yang masih lebih tinggi. Penelitian ini bertujuan untuk meningkatkan pertumbuhan dan menganalisa respon imun udang vannamei L. vannamei yang dipelihara pada media air tawar dengan penambahan mineral kalium dan diuji tantang dengan bakteri vibrio parahaemoliticus. Informasi tentang respon imun tersebut menjadi dasar pengembangan formulasi mineral kalium yang dapat mendukung kesehatan udang sehingga pertumbuhannya dapat berjalan dengan maksimal. Metode penelitian yang digunakan adalah eksperimental menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 5 perlakuan dan 3 ulangan (P1 : Pemeliharaan media air laut (30 ppt); P2 : Suplementasi Kalium 0 mg/L + injeksi vibrio sp; P3 : Suplementasi Kalium 10 mg/L + injeksi vibrio sp; P4 : Suplementasi Kalium 20 mg/L + injeksi vibrio sp; P5 : Suplementasi Kalium 30 mg/L + injeksi vibrio sp.). Berdasarkan penelitian yang telah dilakukan, dapat disimpulkan bahwa dosis penambahan kalium terbaik terjadi pada perlakuan P5 yaitu sebesar Kalium 45 mg/L. Pada dosis tersebut, perlakuan memberikan pengaruh nyata terhadap nilai SR, pertumbuhan, efisiensi pakan, dan sistem imun (THC, DHC, AF). Pada P5, nilai Survival Rate 50%, Pertumbuhan bobot mutlak  34 g, Efisiensi pakan 57%, THC 28 × 106, AF 58 %.
Tocotrienol Berbasis Nabati dalam Akuakultur: Solusi Inovatif untuk Produktivitas, Kesehatan Ikan, dan Keberlanjutan Diamahesa, Wastu Ayu; Azhar, Fariq
JURNAL SAINS TEKNOLOGI & LINGKUNGAN Vol. 11 No. 3 (2025): JURNAL SAINS TEKNOLOGI & LINGKUNGAN
Publisher : LPPM Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jstl.v11i3.864

Abstract

Aquaculture plays a pivotal role in global food security but faces challenges such as diseases, oxidative stress, and reliance on unsustainable feed ingredients. Tocotrienol, a plant-based vitamin E isomer, offers an innovative solution with superior antioxidant, anti-inflammatory, and immunomodulatory properties compared to α-tocopherol. This review examines the utilization of tocotrienol from natural sources like red palm oil (Elaeis guineensis) and annatto seeds (Bixa orellana)—containing up to 4,135 mg/L δ-tocotrienol—to enhance aquaculture productivity. Empirical studies demonstrate that tocotrienol supplementation in feed improves Nile tilapia (Oreochromis niloticus) growth and feed efficiency. Its mechanisms involve NF-κB pathway inhibition, Nrf2-ARE activation, and pparγ gene regulation for lipid metabolism. However, challenges such as thermal degradation during feed extrusion and species-specific responses require formulation strategies like nanoencapsulation or industrial byproducts (PFAD). Through integrated approaches, tocotrienol holds potential as a strategic feed additive to advance sustainable aquaculture by enhancing fish health, product quality, and economic efficiency.
PENINGKATAN PRODUKTIVITAS DAN EFISIENSI BUDIDAYA IKAN NILA MELALUI PENERAPAN PAKAN ALTERNATIF Wolffia arrhiza DI DESA BAGIK POLAK, LOMBOK BARAT Awan Dermawan; Dewi Putri Lestari; Abd Saddam Mujib; Muhammad Junaidi; Bagus Dwi Hari Setyono; Zaenal Abidin; Andre Rachmat Scabra; Rizky Regina Kawirian; Fariq Azhar; Rangga Idris Affandi; Laily Fitriani Mulyani; Sahrul Alim
Jurnal Pengabdian Perikanan Indonesia Vol 6 No 1 (2026): Jurnal Pengabdian Perikanan Indonesia
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppi.v6i1.10171

Abstract

Budidaya ikan nila (Oreochromis niloticus) di Desa Bagik Polak, Kecamatan Labuapi, Kabupaten Lombok Barat menghadapi kendala tingginya biaya produksi akibat ketergantungan pada pakan pabrikan komersial yang harganya terus meningkat. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan produktivitas dan efisiensi produksi pembudidaya ikan nila melalui penerapan pakan alternatif berbasis bahan baku lokal. Kegiatan dilaksanakan pada bulan Agustus 2025 dengan metode penyuluhan teknis dan pendampingan selama satu bulan. Hasil kegiatan menunjukkan peningkatan pemahaman dan keterampilan peserta secara signifikan dalam pengenalan, pemilihan, dan aplikasi bahan pakan alternatif lokal. Penerapan pakan alternatif lokal terbukti mampu menekan biaya pakan sehingga meningkatkan efisiensi produksi tanpa mengurangi kualitas hasil budidaya. Program ini sejalan dengan upaya pemberdayaan masyarakat menuju kemandirian pangan yang berkelanjutan.
Pengaruh Penambahan Serbuk Daun Pepaya pada Pakan untuk Meningkatkan Pertumbuhan Ikan Lele Muhammad Bangun; Muhammad Marzuki; Fariq Azhar
Journal of Aquaculture Science Vol 6 No 2 (2021): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v6i2.141

Abstract

Daun pepaya merupakan bahan alami yang sering dimanfaatkan dibidang perikanan air tawar khususnya sebagai pakan tambahan, karena memiliki kandungan enzim yang dibutuhkan oleh ikan. Penelitian ini bertujuan untuk menganalisis pengaruh penambahan serbuk daun pepaya pada pakan untuk meningkatkan pertumbuhan ikan lele (Clarias sp). Pakan yang digunakan pada penelitian ini yaitu pakan komersil dengan kandungan protein 30% yang ditambahkan serbuk daun pepaya. Serbuk daun pepaya didapatkan dari daun pepaya yang dikeringkan terlebih dahulu sampai benar-benar kering. Setelah kering daun pepaya dihaluskan dengan menggunakan blender. Penelitian dilakukan secara eksperimental menggunakan Rancangan Acak Lengkap (RAL), dengan 4 perlakuan dan 3 ulangan. Perlakuan 1 (P1) sebagai kontrol (tanpa pemberian serbuk daun pepaya), P2 (pakan dengan serbuk daun pepaya 2 %), P3 (Pakan dengan serbuk daun pepaya 3,5 %), P4 (pakan dengan serbuk daun pepaya 4,2 %). Parameter pertumbuhan merupakan parameter utama yang diamati pada penelitian ini. Analisis data menggunakan ANOVA untuk meliihat perbedaan pengaruh dari masing masing perlakuan dengan selang kepercayaan 95% (p>0.05). Hasil penelitian menunjukan bahwa penambahan serbuk daun pepaya memberikan pengaruh nyata terhadap laju pertumbuhan panjang dan berat (p>0.05), dimana perlakuan terbaik terdapat pada P3 dengan pertumbuahan panjang sebesar 2,69 g dan 3,51 cm, berat dan panjang spesifik 3,89 g dan 5,89 cm, serta FCR 0,517.   Kata kunci: pertumbuhan, serbuk, daun papaya
Response of Neem Leaves Azadirchta indica Extract Immune System of (Oreochromis niloticus) Lutfi Hamzan Wari; Ayu Adhita Damayanti; Fariq Azhar
Journal of Aquaculture Science Vol 5 No 1 (2020): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v5i1.83

Abstract

Tilapia (Oreochromis niloticus) is a species of freshwater fish that is widely cultivated in areas that have a large quantity of fresh water. In the cultivation of tilapia fish are often infected by fish diseases which not infrequently thwart the growth and survival of fish resulting in death of fish that are cultivated (crop failure). This study aims to analyze the response of giving neem leaf extract (Azadiracta indicida) to the immune system and growth of tilapia. The fish were given neem leaf extract which was added to the feed for 45 days in 54cm x 37cm x 29cm containers each with 10 heads / container with a volume of 20 liters. The study was conducted in 5 treatments, control + (commercial feed and infected by Aeromonas hydrophila 106 cfu / ml as much as 0.1 ml / head), control - (commercial feed of bacterial infection), P1 (feed mixed with 0.5% extra neem leaves and infected with bacteria ), P2 (1% extracted and infected), P3 (2% extracted and infected). The results showed that administration of neem leaf extract at a dose of 1% was able to maintain SR by 100%, increasing the number of erythrocyte tilapia by 15.0 í­” 105 cells / ml and leukocytes by 15.0 í­” 104 cells / ml and hemoglobin by 6.0 % of the total blood cells contained in P4 that play a role in the immune system of tilapia. Based on the results of this study, neem leaf extract can be used in tilapia aquaculture as an immunostimulant because it can improve survival, red blood cells, white blood cells and hemoglobin.Keyword : Extract neem leaves, Aeromonas hydrophila, Tilapia, Feed.
Co-Authors Abd Saddam Mujib Abdul Syukur Abidin, Z Agustin, Pradita Ayu Aini , Kuratul Alis Mukhlis Amanda, Dwika Melani Andre Rachmat Scabra Awan Dermawan Ayu Adhita Damayanti Badrun Badrun Bagus Dwi Hari Setyono Batun Citra Rahmadani, Thoy Damai Diniariwisan Dewi Nur'aini Setyowati Dewi Putri Lestari, Dewi Putri Diamahesa, Wastu Ayu Diniariwisan, Damai Dwina Angga, Prayogi Dwiyanti , Septiana Dyke Gita Wirasisya Eviana, Maulani Fadillah, Hayatun Farman, Yudi Faturrahman Febriana, Nurlita Febrianti, Baiq Yunita Hadzriil Jeeffry , Ku Hafizah, Imroatul Hapizah, Imro'atul I Wayan Rainbow Idris Affandi, Rangga Indarwati Indarwati Irmadiati, Irmadiati Iskandar, M Sopiyan Jaelani, Muhamad Mahsun Junaidi, Muhammad Laily Fitriani Mulyani Laksono Trisnantoro Lumbessy, Salnida Yuniarti Lutfi Hamzan Wari Maknun, Lu'lu'il Marniati Marniati Muhammad Amin Muhammad Bangun Muhammad Bangun Mubaraq Muhammad Junaidi Muhammad Junaidi Muhammad Marzuki Muhammad Sumsanto Mulyani, Laily Nanang Satria Sumarjan Nanda Diniarti Nur Fadillah Nur Fadillah, Nur Nurhasanah Nurhasanah Nuri Muahiddah Nurliah Nurliah Nurul Pratiwi Rabi’atussamiah Rahmadani, Thoy Batun Citra Rahman, Ibadur Rangga Idris Affandi Rizky Regina Kawirian Rosyida, Azilia Sahrul Alim Saptono Waspodo Setyowati, Dewi Nur'aeni Setyowati, Dewi Nur’aeni Setyowati, Dewi Nur’aeni Siti Hilyana Soleha, Anisa R. Syafitri, Iin Syawalina Fitria Thoy Batun Citra Rahmadani Widia Lestari Wilisetyadi, Lalu Wahyu yadin yadin, yadin Yahya, Aslam Zulfiqar Yuliana Asri Zaenal Abidin Zaenal Abidin