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Efektivitas Ekstrak Daun Komak (Lablab purpureus) Terhadap Sistem Imun Ikan Nila (Oreochromis niloticus) Yang Diinjeksi Bakteri Aeromonas hydrophila Rabi’atussamiah; Azhar, Fariq; Abidin, Zaenal; setyowati, Dewi Nur’aeni
Jurnal Media Akuakultur Indonesia Vol 4 No 4 (2024): Indonesian Journal of Aquaculture Medium
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/mediaakuakultur.v4i4.6140

Abstract

This study aims to determine the effect of comma leaf extract on the immune system of tilapia injected with A. hydrophila bacteria. This research was conducted for 60 days at the Fish Production and Reproduction Laboratory and Aquaculture Fish Health Laboratory, Aquaculture Study Program, Department of Fisheries and Marine Sciences, Faculty of Agriculture, University of Mataram. The method used was a completely randomized design (CRD) experiment with five treatments and three replications, namely where K- was not given comma leaf extract and was injected with 0.9% NaCl, K+ was not given comma leaf extract and was injected with A. Hydrophila bacteria, P1 (0.5%) extract comma leaf and injected with A. hydrophila bacteria, P2 (1%) of komak leaf extract and injected with A. hydophila bacteria, P3 (2%) of komak leaf extract and injected with A. hydrophila bacteria. The observed parameters were total erythrocytes, total leukocytes, hemoglobin, hematocrit, differential leukocytes, phagocytosis activity, total bacteria and survival (SR). The results showed that the average value of total erythrocytes was 1.22-1.82 x106 cells/mm3, total leukocytes were 2.08-2.8 x104 cells/mm3, hemoglobin was 3.4-5.5%, hematocrit was 14.1-17.3%, differential leukocytes consisted of 4 types of cells, namely lymphocytes. , monocytes, neutrophils and platelets. Lymphocytes 60.7-72.3%, monocytes 4.3-7.3%, neutrophils 17-24.3%, and platelets 6.3-7.7%, phagocytosis activity 44.8-66.3%, total bacteria (TB) OD620 3.39-3.84, survival (SR) 46.7-66.7 %. The addition of 2% of comma leaf extract to the feed can improve the immune system of tilapia injected with A. hydrophila bacteria
Growth of Sea Grapes (Caulerpa lentillifera) in Laboratory-Scale Cultivation With Urea As A Single Nitrogen Nutrient Eviana, Maulani; Mukhlis, Alis; Azhar, Fariq
Journal of Fish Health Vol. 4 No. 4 (2024): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v4i4.5956

Abstract

Sea grape (Caulerpa lentillifera) is a strategic green macroalgae with significant potential in aquaculture and functional food industries. However, its cultivation remains constrained by limited production techniques. This study investigated the influence of nitrogen concentration on C. lentillifera growth in a laboratory-scale cultivation system. A completely randomized experimental design was implemented with four treatments: a control group (0 ppm) and nitrogen supplementation levels of 5, 10, and 15 ppm, each replicated thrice over 21 days. Results demonstrated a significant positive correlation between nitrogen concentration and growth parameters. The 15 ppm nitrogen treatment exhibited the highest absolute growth (3.37 g), relative growth rate (13.50%), and specific growth rate (1.82% per day). Interestingly, no statistically significant difference was observed between 10 and 15 ppm treatments, suggesting a potential nitrogen uptake saturation point. Water quality parameters remained stable throughout the experiment, with temperature ranging from 28-30°C, salinity at 33-34 ppt, dissolved oxygen between 5.9-6.7 ppm, and pH maintained at 7.5-7.8. The study provides crucial insights into nitrogen's role in C. lentillifera cultivation, offering a scientific foundation for developing more efficient and sustainable macroalgae production strategies in aquaculture and functional food industries.
Efficiency of Using Kappa-Carrageenan as an Immunostimulant Agent in the Non-Specific Immune System of Vannamei (Litopenaeus vannamei) Infected with AHPND (Acute Hepatopancreatic Necrosis Disease) Azhar, Fariq; Scabra, Andre Rachmat; Muahiddah, Nuri; Affandi, Rangga Idris
Journal of Fish Health Vol. 5 No. 1 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i1.6358

Abstract

Vibrio parahaemolyticus is one of the bacteria that can attack whiteleg shrimp and cause Acute Hepatopancreatic Necrosis Disease (AHPND) or Early Mortality Syndrome (EMS) in shrimp. One alternative that can be used is the use of seaweed-based immunostimulants, namely k-carrageenan. The purpose of this study was to evaluate the effectiveness of k-carrageenan mixed in feed with different doses on increasing the immune system of whiteleg shrimp infected with AHPND. This study was conducted for 60 days with an experimental method in the form of a Completely Randomized Design (CRD) consisting of 5 treatments and 3 replications, namely, P1 (Positive Control): Commercial Feed + Vibrio parahaemolyticus Infection; P2 (Negative Control): Commercial Feed + 0.9% NaCl Infection; P3: Commercial Feed + Kappa-carrageenan 8 g / kg + Vibrio parahaemolyticus Infection; P4: Commercial Feed + Kappa-carrageenan 10 g/kg + Vibrio parahaemolyticus Infection; P5: Commercial Feed + Kappa-carrageenan 12 g/kg + Vibrio parahaemolyticus Infection. The results of this study indicate that the addition of 12 g/kg of k-carrageenan to the feed affects the survival rate and immune system of whiteleg shrimp infected with Vibrio parahaemolyticus.
Hematological Parameters and Growth Performance of Nile Tilapia (Oreochromis niloticus) Fed Fermented Papaya (Carica papaya L.) Leaf Extract Lestari, Widia; Azhar, Fariq; Diamahesa, Wastu Ayu
Journal of Fish Health Vol. 5 No. 4 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i4.8335

Abstract

Nile tilapia (Oreochromis niloticus) is one of the most important aquaculture species worldwide, but intensive farming has increased susceptibility to bacterial infections, particularly Aeromonas hydrophila, which threatens productivity and survival. This study evaluated fermented papaya (Carica papaya L.) leaf extract (FPLE) as a natural immunostimulant and sustainable alternative to antibiotics in Nile tilapia culture. A 45-day feeding trial was conducted using a completely randomized design with five treatments (0, 16, 18, and 20 mL FPLE/kg feed) and three replicates. Hematological, immunological, growth, and bacterial resistance parameters were analyzed. Results showed that supplementation with 20 mL/kg FPLE significantly increased post-infection leukocyte counts (2.82×10⁴ vs. 2.40×10⁴ cells/mm³), phagocytic activity (46.26% vs. 39.51%), and erythrocyte levels (5.85×10⁶ vs. 5.25×10⁶ cells/mm³). Differential leukocyte analysis revealed lymphocyte dominance (90.33%) and elevated monocytes (13.33%), indicating enhanced adaptive and nonspecific immunity. In addition, total bacterial load decreased by 22.4%, and specific growth rate improved (4.34% vs. 4.22%/day), while water quality remained within optimal ranges. The synergistic effects of papaya-derived bioactive compounds and Lactobacillus casei probiotics from Yakult likely contributed to immune enhancement and nutrient absorption. FPLE supplementation at 20 mL/kg optimized immunostimulation without compromising survival (77–84%), demonstrating its dual role in disease prevention and growth promotion. These findings highlight FPLE as a safe, eco-friendly feed additive with strong potential for reducing antibiotic dependence and supporting sustainable tilapia aquaculture.
Feed production techniques using local raw materials for mud crab farmers in Madak Belek Hamlet, Sekotong District Diamahesa, Wastu Ayu; Lumbessy, Salnida Yuniarti; Azhar, Fariq; Abidin, Zaenal; Badrun, Badrun; Affandi, Rangga Idris; Sumsanto, Muhammad; Diniariwisan, Damai; Rahmadani, Thoy Batun Citra; Setyono, Bagus Dwi Hari
Penamas: Journal of Community Service Vol. 5 No. 4 (2025): Penamas: Journal of Community Service
Publisher : Nur Science Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53088/penamas.v5i4.2284

Abstract

This community service activity focused on developing feed production techniques using local raw materials for mud crab farmers in Madak Belek Hamlet, Sekotong District. The main challenge faced by farmers is the high cost of commercial feed, which reduces profit margins. In fact, the area is rich in underutilized nutrient-rich local resources such as trash fish and seafood processing by-products. A participatory approach was applied, including observation, socialization, demonstration, hands-on training, and evaluation. The training emphasized feed formulation and the application of 5% wheat gluten as a binder, which had been proven in preliminary studies to enhance feed stability in water. The results showed an improvement in participants’ knowledge and skills in processing local ingredients into quality feed. The expected impacts include reducing dependence on commercial feed, lowering production costs, and improving the efficiency and sustainability of mud crab farming. For further optimization, feed performance testing on crab growth and economic feasibility analysis are required.
PENINGKATAN PRODUKTIVITAS DAN EFISIENSI BUDIDAYA IKAN NILA MELALUI PENERAPAN PAKAN ALTERNATIF Wolffia arrhiza DI DESA BAGIK POLAK, LOMBOK BARAT Awan Dermawan; Dewi Putri Lestari; Abd Saddam Mujib; Muhammad Junaidi; Bagus Dwi Hari Setyono; Zaenal Abidin; Andre Rachmat Scabra; Rizky Regina Kawirian; Fariq Azhar; Rangga Idris Affandi; Laily Fitriani Mulyani; Sahrul Alim
Indonesian Journal of Fisheries Community Empowerment Vol 6 No 1 (2026): Jurnal Pengabdian Perikanan Indonesia
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppi.v6i1.10171

Abstract

Budidaya ikan nila (Oreochromis niloticus) di Desa Bagik Polak, Kecamatan Labuapi, Kabupaten Lombok Barat menghadapi kendala tingginya biaya produksi akibat ketergantungan pada pakan pabrikan komersial yang harganya terus meningkat. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan produktivitas dan efisiensi produksi pembudidaya ikan nila melalui penerapan pakan alternatif berbasis bahan baku lokal. Kegiatan dilaksanakan pada bulan Agustus 2025 dengan metode penyuluhan teknis dan pendampingan selama satu bulan. Hasil kegiatan menunjukkan peningkatan pemahaman dan keterampilan peserta secara signifikan dalam pengenalan, pemilihan, dan aplikasi bahan pakan alternatif lokal. Penerapan pakan alternatif lokal terbukti mampu menekan biaya pakan sehingga meningkatkan efisiensi produksi tanpa mengurangi kualitas hasil budidaya. Program ini sejalan dengan upaya pemberdayaan masyarakat menuju kemandirian pangan yang berkelanjutan.
Pengaruh Penambahan Serbuk Daun Pepaya pada Pakan untuk Meningkatkan Pertumbuhan Ikan Lele Muhammad Bangun; Muhammad Marzuki; Fariq Azhar
Journal of Aquaculture Science Vol 6 No 2 (2021): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v6i2.141

Abstract

Daun pepaya merupakan bahan alami yang sering dimanfaatkan dibidang perikanan air tawar khususnya sebagai pakan tambahan, karena memiliki kandungan enzim yang dibutuhkan oleh ikan. Penelitian ini bertujuan untuk menganalisis pengaruh penambahan serbuk daun pepaya pada pakan untuk meningkatkan pertumbuhan ikan lele (Clarias sp). Pakan yang digunakan pada penelitian ini yaitu pakan komersil dengan kandungan protein 30% yang ditambahkan serbuk daun pepaya. Serbuk daun pepaya didapatkan dari daun pepaya yang dikeringkan terlebih dahulu sampai benar-benar kering. Setelah kering daun pepaya dihaluskan dengan menggunakan blender. Penelitian dilakukan secara eksperimental menggunakan Rancangan Acak Lengkap (RAL), dengan 4 perlakuan dan 3 ulangan. Perlakuan 1 (P1) sebagai kontrol (tanpa pemberian serbuk daun pepaya), P2 (pakan dengan serbuk daun pepaya 2 %), P3 (Pakan dengan serbuk daun pepaya 3,5 %), P4 (pakan dengan serbuk daun pepaya 4,2 %). Parameter pertumbuhan merupakan parameter utama yang diamati pada penelitian ini. Analisis data menggunakan ANOVA untuk meliihat perbedaan pengaruh dari masing masing perlakuan dengan selang kepercayaan 95% (p>0.05). Hasil penelitian menunjukan bahwa penambahan serbuk daun pepaya memberikan pengaruh nyata terhadap laju pertumbuhan panjang dan berat (p>0.05), dimana perlakuan terbaik terdapat pada P3 dengan pertumbuahan panjang sebesar 2,69 g dan 3,51 cm, berat dan panjang spesifik 3,89 g dan 5,89 cm, serta FCR 0,517.   Kata kunci: pertumbuhan, serbuk, daun papaya
Response of Neem Leaves Azadirchta indica Extract Immune System of (Oreochromis niloticus) Lutfi Hamzan Wari; Ayu Adhita Damayanti; Fariq Azhar
Journal of Aquaculture Science Vol 5 No 1 (2020): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v5i1.83

Abstract

Tilapia (Oreochromis niloticus) is a species of freshwater fish that is widely cultivated in areas that have a large quantity of fresh water. In the cultivation of tilapia fish are often infected by fish diseases which not infrequently thwart the growth and survival of fish resulting in death of fish that are cultivated (crop failure). This study aims to analyze the response of giving neem leaf extract (Azadiracta indicida) to the immune system and growth of tilapia. The fish were given neem leaf extract which was added to the feed for 45 days in 54cm x 37cm x 29cm containers each with 10 heads / container with a volume of 20 liters. The study was conducted in 5 treatments, control + (commercial feed and infected by Aeromonas hydrophila 106 cfu / ml as much as 0.1 ml / head), control - (commercial feed of bacterial infection), P1 (feed mixed with 0.5% extra neem leaves and infected with bacteria ), P2 (1% extracted and infected), P3 (2% extracted and infected). The results showed that administration of neem leaf extract at a dose of 1% was able to maintain SR by 100%, increasing the number of erythrocyte tilapia by 15.0 í­” 105 cells / ml and leukocytes by 15.0 í­” 104 cells / ml and hemoglobin by 6.0 % of the total blood cells contained in P4 that play a role in the immune system of tilapia. Based on the results of this study, neem leaf extract can be used in tilapia aquaculture as an immunostimulant because it can improve survival, red blood cells, white blood cells and hemoglobin.Keyword : Extract neem leaves, Aeromonas hydrophila, Tilapia, Feed.
The Addition of Mangrove Leaf Extract Rhizophora apiculata in White Shrimp (Litopenaeus vannamei) for Vibriosis Prevention Nur Fadillah; Saptono Waspodo; Fariq Azhar
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.75

Abstract

White shrimp is a brackish water commodity which is a solution to approve tiger shrimp (Penaeus monodon) which improves quality. One of the challenges in the cultivation of white shrimp is the attack of vibriosis, caused by the vibrio genus species V. parahaemolyticus. Rhizophora apiculata mangrove leaf extract with different dosages in white shrimp feed to increase vibriosis. Shrimp were given mangrove leaf extract for 40 days and were challenged for 7 days in a 40cm x 30cm x 28cm container, 20 heads/20 liter volume container. The study was conducted in 5 training, control + (Feed without extract + Infection), control- (feed without extract), P3 (0,5% + Infection), P4 (1% extract + Infection), P5 (2% extract + Infection ), the injected bacterial dose is 0.1 ml/heads density of 106 cfu/ml. The results showed that administration of mangrove leaf extract at a dose of 2% was able to maintain SR of 76,67%, increasing shrimp THC by 7,55í­”106 cells/ml and DHC (hyaline cells 84,3% and granular 15,3%) which plan in the shrimp immune system. The number of bacteria and the amount of vibrio in the intestine is, 64,7í­”108 cfu/ml and 16í­”108 cfu/ml. Based on the results of the study, the use of mangrove leaf extract can be used in the cultivation of white shrimp as an immunostimulant because it can increase survival, THC, DHC and reduce bacteria in the intestines of white shrimp. Keywords: extracts, mangrove leaves, vibriosis, white shrimp, feed.
Pelatihan Pembuatan Kepiting Presto Crispy Sebagai Diversifikasi Produk Pangan Berbasis Potensi Pesisir Di Kawasan Ekowisata Bagek Kembar, Sekotong Wastu Ayu Diamahesa; Bagus Dwi Hari Setyono; Muhammad Baiul Hak; Fariq Azhar; Yesica Marcelina Romauli Sinaga; Zaenal Abidin; Laily Fitriani Mulyani; Yuliana Asri; Sahrul Alim; Dewi Putri Lestari
Jurnal Pengabdian Magister Pendidikan IPA Vol 9 No 2 (2026): April-Juni 2026
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v9i2.15816

Abstract

Abstract: Coastal areas have considerable potential to support community-based food product innovation, particularly through the utilization of local fishery resources. This community service activity aimed to improve the knowledge and practical skills of POKDARWIS Bagek Kembar in processing crab into a value-added food product, namely crispy pressure-cooked crab. The activity was carried out in Bagek Kembar Ecotourism Area, Cendi Manik Village, Sekotong District, West Lombok Regency, West Nusa Tenggara, from July to September 2025. The program involved preliminary identification, partner needs assessment, counseling, demonstration, and hands-on training. A total of 15 participants joined the training. The material covered raw material preparation, crab cleaning, basic food hygiene, pressure-cooking technique, seasoning, flour coating, and frying process to produce a crispy texture. The results showed that the participants were actively involved in discussions and practical sessions and were able to follow the main stages of crispy pressure-cooked crab processing. This activity introduced a simple and applicable processing technique that can potentially support local culinary diversification in the ecotourism area. Further assistance is needed, particularly in product standardization, food safety, packaging, marketing, and business feasibility analysis.
Co-Authors Abd Saddam Mujib Abdul Syukur Abidin, Z Abidin, Zaenal Agustin, Pradita Ayu Aini , Kuratul Alis Mukhlis Amanda, Dwika Melani Andre Rachmat Scabra Awan Dermawan Ayu Adhita Damayanti Badrun Badrun Bagus Dwi Hari Setyono Damai Diniariwisan Dewi Nur'aini Setyowati Dewi Putri Lestari, Dewi Putri Diamahesa, Wastu Ayu Diniariwisan, Damai Dwina Angga, Prayogi Dwiyanti , Septiana Dyke Gita Wirasisya Eviana, Maulani Fadillah, Hayatun Farman, Yudi Faturrahman Febriana, Nurlita Febrianti, Baiq Yunita Hadzriil Jeeffry , Ku Hafizah, Imroatul Hapizah, Imro'atul I Wayan Rainbow Idris Affandi, Rangga Indarwati Indarwati Irmadiati, Irmadiati Jaelani, Muhamad Mahsun Junaidi, Muhammad Laily Fitriani Mulyani Laily Fitriani Mulyani Laksono Trisnantoro Lumbessy, Salnida Yuniarti Lutfi Hamzan Wari Maknun, Lu'lu'il Marniati Marniati Muhammad Amin Muhammad Baiul Hak Muhammad Bangun Muhammad Bangun Mubaraq Muhammad Junaidi Muhammad Junaidi Muhammad Marzuki Muhammad Sumsanto Mulyani, Laily Nanang Satria Sumarjan Nanda Diniarti Nur Fadillah Nur Fadillah, Nur Nurhasanah Nurhasanah Nuri Muahiddah Nurliah Nurliah Nurul Pratiwi Rabi’atussamiah Rahmadani, Thoy Batun Citra Rahman, Ibadur Rangga Idris Affandi Rizky Regina Kawirian Rosyida, Azilia Sahrul Alim Saptono Waspodo Setyowati, Dewi Nur'aeni Setyowati, Dewi Nur’aeni Setyowati, Dewi Nur’aeni Siti Hilyana Soleha, Anisa R. Syafitri, Iin Syawalina Fitria Widia Lestari Wilisetyadi, Lalu Wahyu yadin yadin, yadin Yahya, Aslam Zulfiqar Yesica Marcelina Romauli Sinaga Yuliana Asri Yuliana Asri Zaenal Abidin Zaenal Abidin