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Hematological Parameters and Growth Performance of Nile Tilapia (Oreochromis niloticus) Fed Fermented Papaya (Carica papaya L.) Leaf Extract Lestari, Widia; Azhar, Fariq; Diamahesa, Wastu Ayu
Journal of Fish Health Vol. 5 No. 4 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i4.8335

Abstract

Nile tilapia (Oreochromis niloticus) is one of the most important aquaculture species worldwide, but intensive farming has increased susceptibility to bacterial infections, particularly Aeromonas hydrophila, which threatens productivity and survival. This study evaluated fermented papaya (Carica papaya L.) leaf extract (FPLE) as a natural immunostimulant and sustainable alternative to antibiotics in Nile tilapia culture. A 45-day feeding trial was conducted using a completely randomized design with five treatments (0, 16, 18, and 20 mL FPLE/kg feed) and three replicates. Hematological, immunological, growth, and bacterial resistance parameters were analyzed. Results showed that supplementation with 20 mL/kg FPLE significantly increased post-infection leukocyte counts (2.82×10⁴ vs. 2.40×10⁴ cells/mm³), phagocytic activity (46.26% vs. 39.51%), and erythrocyte levels (5.85×10⁶ vs. 5.25×10⁶ cells/mm³). Differential leukocyte analysis revealed lymphocyte dominance (90.33%) and elevated monocytes (13.33%), indicating enhanced adaptive and nonspecific immunity. In addition, total bacterial load decreased by 22.4%, and specific growth rate improved (4.34% vs. 4.22%/day), while water quality remained within optimal ranges. The synergistic effects of papaya-derived bioactive compounds and Lactobacillus casei probiotics from Yakult likely contributed to immune enhancement and nutrient absorption. FPLE supplementation at 20 mL/kg optimized immunostimulation without compromising survival (77–84%), demonstrating its dual role in disease prevention and growth promotion. These findings highlight FPLE as a safe, eco-friendly feed additive with strong potential for reducing antibiotic dependence and supporting sustainable tilapia aquaculture.
Feed production techniques using local raw materials for mud crab farmers in Madak Belek Hamlet, Sekotong District Diamahesa, Wastu Ayu; Lumbessy, Salnida Yuniarti; Azhar, Fariq; Abidin, Zaenal; Badrun, Badrun; Affandi, Rangga Idris; Sumsanto, Muhammad; Diniariwisan, Damai; Rahmadani, Thoy Batun Citra; Setyono, Bagus Dwi Hari
Penamas: Journal of Community Service Vol. 5 No. 4 (2025): Penamas: Journal of Community Service
Publisher : Nur Science Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53088/penamas.v5i4.2284

Abstract

This community service activity focused on developing feed production techniques using local raw materials for mud crab farmers in Madak Belek Hamlet, Sekotong District. The main challenge faced by farmers is the high cost of commercial feed, which reduces profit margins. In fact, the area is rich in underutilized nutrient-rich local resources such as trash fish and seafood processing by-products. A participatory approach was applied, including observation, socialization, demonstration, hands-on training, and evaluation. The training emphasized feed formulation and the application of 5% wheat gluten as a binder, which had been proven in preliminary studies to enhance feed stability in water. The results showed an improvement in participants’ knowledge and skills in processing local ingredients into quality feed. The expected impacts include reducing dependence on commercial feed, lowering production costs, and improving the efficiency and sustainability of mud crab farming. For further optimization, feed performance testing on crab growth and economic feasibility analysis are required.
IMPROVING THE HEALTH OF VANNAMEI SHRIMP (Penaeus Vanname) MAINTAINED IN LOW-SALINITY MEDIA WITH POTASSIUM MINERAL SUPPLEMENTATION Scabra, Andre Rachmat; Azhar, Fariq; Affandi, Rangga Idris; Iskandar, M Sopiyan
Jurnal Perikanan Unram Vol 15 No 6 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i6.1056

Abstract

Penambahan berbagai jenis mineral pada kegiatan budidaya udang vannamei media air tawar diketahui menghasilkan pertumbuhan udang vannamei yang lebih tinggi. Namun demikian, pertumbuhan udang vannamei pada habitat aslinya, yaitu air laut, masih menghasilkan pertumbuhan yang masih lebih tinggi. Penelitian ini bertujuan untuk meningkatkan pertumbuhan dan menganalisa respon imun udang vannamei L. vannamei yang dipelihara pada media air tawar dengan penambahan mineral kalium dan diuji tantang dengan bakteri vibrio parahaemoliticus. Informasi tentang respon imun tersebut menjadi dasar pengembangan formulasi mineral kalium yang dapat mendukung kesehatan udang sehingga pertumbuhannya dapat berjalan dengan maksimal. Metode penelitian yang digunakan adalah eksperimental menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 5 perlakuan dan 3 ulangan (P1 : Pemeliharaan media air laut (30 ppt); P2 : Suplementasi Kalium 0 mg/L + injeksi vibrio sp; P3 : Suplementasi Kalium 10 mg/L + injeksi vibrio sp; P4 : Suplementasi Kalium 20 mg/L + injeksi vibrio sp; P5 : Suplementasi Kalium 30 mg/L + injeksi vibrio sp.). Berdasarkan penelitian yang telah dilakukan, dapat disimpulkan bahwa dosis penambahan kalium terbaik terjadi pada perlakuan P5 yaitu sebesar Kalium 45 mg/L. Pada dosis tersebut, perlakuan memberikan pengaruh nyata terhadap nilai SR, pertumbuhan, efisiensi pakan, dan sistem imun (THC, DHC, AF). Pada P5, nilai Survival Rate 50%, Pertumbuhan bobot mutlak  34 g, Efisiensi pakan 57%, THC 28 × 106, AF 58 %.
Tocotrienol Berbasis Nabati dalam Akuakultur: Solusi Inovatif untuk Produktivitas, Kesehatan Ikan, dan Keberlanjutan Diamahesa, Wastu Ayu; Azhar, Fariq
JURNAL SAINS TEKNOLOGI & LINGKUNGAN Vol. 11 No. 3 (2025): JURNAL SAINS TEKNOLOGI & LINGKUNGAN
Publisher : LPPM Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jstl.v11i3.864

Abstract

Aquaculture plays a pivotal role in global food security but faces challenges such as diseases, oxidative stress, and reliance on unsustainable feed ingredients. Tocotrienol, a plant-based vitamin E isomer, offers an innovative solution with superior antioxidant, anti-inflammatory, and immunomodulatory properties compared to α-tocopherol. This review examines the utilization of tocotrienol from natural sources like red palm oil (Elaeis guineensis) and annatto seeds (Bixa orellana)—containing up to 4,135 mg/L δ-tocotrienol—to enhance aquaculture productivity. Empirical studies demonstrate that tocotrienol supplementation in feed improves Nile tilapia (Oreochromis niloticus) growth and feed efficiency. Its mechanisms involve NF-κB pathway inhibition, Nrf2-ARE activation, and pparγ gene regulation for lipid metabolism. However, challenges such as thermal degradation during feed extrusion and species-specific responses require formulation strategies like nanoencapsulation or industrial byproducts (PFAD). Through integrated approaches, tocotrienol holds potential as a strategic feed additive to advance sustainable aquaculture by enhancing fish health, product quality, and economic efficiency.