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Cluster Analysis and SSR (Simple Sequence Repeats) Primers Selection for Aluminium Tolerance on Hot Pepper Catur Herison; Rustikawati; Ria Meita; Hasanudin
Jurnal Hortikultura Indonesia Vol. 11 No. 1 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.1.61-71

Abstract

Perakitan varietas unggul cabai merah toleran Al (Aluminium) merupakan solusi terbaik untuk mengatasi persoalan rendahnya produkvitas cabai di lahan kering masam. Identifikasi tetua toleran dan marka SSR (Simple Sequence Repeats) sangat bermanfaat dalam meningkatkan efektifitas program pemuliaan tanaman untuk merakit varietas toleran terhadap cekaman aluminium. Seleksi primer SSR adalah bagian awal yang penting dalam pengembangan marka molekuler tersebut. Penelitian bertujuan untuk menentukan kelompok genotipe toleran alumunium dan menentukan primer prospektif dalam pengembangan marka SSR untuk sifat toleran terhadap Al pada tanaman cabai. Evaluasi tingkat toleransi 27 genotipe cabai terhadap cekaman aluminium dilakukan menggunakan hidroponik sistem sumbu dengan larutan hara AB mix ditambah 500 ppm AlCl3.6H2O. Tahap selanjutnya adalah menguji 20 primer SSR dalam mengamplifikasi DNA genom cabai toleran dan peka yang mewakili. Hasil analisis cluster sifat toleransi terhadap 27 genotipe cabai menghasilkan 7 kelompok tingkat toleransi terhadap aluminium. Individu PBC396 dan PBC518 adalah genotipe yang sangat toleran terhadap cekaman aluminium yang unik sehingga keduanya sangat baik digunakan sebagai tetua dalam pengembangan cabai toleran aluminium. Primer SSR yang potensial menghasilkan marka polimorfik untuk sifat toleran terhadap cekaman aluminium pada cabai adalah TMS7 dan CAMS358. Primer tersebut selanjutnya harus diuji pada mapping population untuk menentukan pita yang dapat digunakan sebagai penanda toleransi terhadap aluminium. Kata kunci: Capsicum annuum, NTSYS, sidik gerombol, tenggang masam
Status Keberlanjutan Wilayah Berbasis Peternakan di Kabupaten Situbondo untuk Pengembangan Kawasan Agropolitan Suyitman Suyitman; Surjono Hadi Sutjahjo; Catur Herison; nFN Muladno
Jurnal Agro Ekonomi Vol 27, No 2 (2009): Jurnal Agro Ekonomi
Publisher : Pusat Sosial Ekonomi dan Kebijakan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jae.v27n2.2009.165-191

Abstract

EnglishThe government of Situbondo Regency has not decided the location of  the agropolitan development. In this regard, a study on livestock-based regional sustainability development is necessary before the implementation of the agropolitan program  This study aims to analyze the sustainability index and the status of Situbondo area through five dimensions of sustainability. The study used a Multidimensional Scaling analysis (MDS) method called Rap-BANGKAPET and the results are shown in the form of sustainability index and status. The Leverage and Monte Carlo analyses were used to determine the attributes that sensitively affect the index and the status of sustainability and its impact. Sustainability analysis indicate that the ecological dimension is less sustainable (46.50%), economic dimention is sufficiently sustainable (69.53%), social and cultural dimensions is also sufficiently sustainable (55.14%), infrastructure and technology dimensions are at low level of sustainability (45.48%), and similarly at low level of sustainability for legal and institutional dimensions (47.46%). Of the 73 attributes, 24 of these attributes require direct treatment immediately because of its sensitive effect on sustainability index and status (significant at 95% of confidence level). The study suggests a progressive-optimistic scenario to improve the sustainability of the future status (long term) with overall improvement of sensitive attributes. IndonesianPemerintah Daerah Tingkat II Kabupaten Situbondo sampai saat ini masih belum menetapkan wilayahnya untuk pengembangan kawasan agropolitan. Untuk itu sebelum program agropolitan dilaksanakan perlu dikaji terlebih dahulu tingkat keberlanjutan wilayah berbasis peternakan di Kabupaten Situbondo. Penelitian ini bertujuan untuk menganalisis indeks dan status keberlanjutan wilayah berbasis peternakan di Kabupaten Situbondo ditinjau dari lima dimensi keberlanjutan, yaitu: dimensi ekonomi, sosial budaya, infrastruktur dan teknologi, serta hukum dan kelembagaan. Penelitian meggunakan metode analisis Multidimensional Scaling (MDS) yang disebut RAP-BANGKAPET dan hasilnya dinyatakan dalam bentuk indeks dan status keberlanjutan. Untuk mengetahui atribut yang sensitif berpengaruh terhadap indeks dan status keberlanjutan dan pengaruh galat dilakukan analisis Leverage dan Monte Carlo.   Hasil analisis keberlanjutan menunjukkan bahwa dimensi ekologi berada pada status kurang berkelanjutan (46,50%), dimensi ekonomi cukup berkelanjutan (69,53%), dimensi sosial budaya cukup berkelanjutan (55,14%), dimensi infrastruktur dan teknologi kurang berkelanjutan (45,48%), serta dimensi hukum dan kelembagaan kurang berkelanjutan (47,46%). Dari 73 atribut yang dianalisis, 24 atribut yang perlu segera ditangani karena sensitif berpengaruh terhadap peningkatan indeks dan status keberlanjutan dengan tingkat galat (error) yang sangat kecil pada taraf kepercayaan 95 persen. Dalam rangka meningkatkan status keberlanjutan ke depan (jangka panjang), skenario yang perlu dilakukan adalah skenario progresif-optimistik dengan melakukan perbaikan secara menyeluruh terhadap semua atribut yang sensitif dalam peningkatan status kawasan.
Analisis Keberlanjutan Wilayah Perbatasan Kalimantan Barat-Malaysia untuk Pengembangan Kawasan Agropolitan (Studi Kasus Kecamatan Dekat Perbatasan Kabupaten Bengkayang) nFN Thamrin; Surjono H. Sutjahjo; Catur Herison; Supiandi Sabiham
Jurnal Agro Ekonomi Vol 25, No 2 (2007): Jurnal Agro Ekonomi
Publisher : Pusat Sosial Ekonomi dan Kebijakan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jae.v25n2.2007.103-124

Abstract

EnglishThe Government of Bengkayang Regency has decided that the border area is one of the agropolitan region development programs. Hence, a study on the sustainability level of border area as the agropolitan region development is required.  The first objective of this study was to analyze the index and sustainability status of the border area, Bengkayang Regency, based on five sustainable dimensions. The analysis used Multi Dimensional Scaling (MDS) Method, called Rap-BENGKAWAN and the results were stated in the index and sustainability status. The second objective was to analyze the attributes that affect sensitively on index and sustainability status and the effect of error using Laverage and Monte Carlo Analysis. The results of the study revealed that ecological dimension was in the status of less sustainable (40.37%), economical dimension was sufficient sustainable (66.54%), socio-culture dimension was sufficient sustainable (67.06%), dimension of infrastructure and technology was not sustainable (24.49%) and dimension of law and institutional was sufficient sustainable (60,10).  Out of the 47 attributes analyzed, there were 22 attributes need to be handled immediately as they affect sensitively on the increase of index and sustainability status with a negligible error in the level of 95% confidence limit. IndonesianPemerintah Kabupaten Bengkayang menetapkan wilayah perbatasan sebagai salah satu program pengembangan kawasan agropolitan. Untuk itu, perlu dikaji tingkat keberlanjutan wilayah perbatasan sebagai kawasan pengembangan agropolitan. Penelitian ini bertujuan untuk menganalisis indeks dan status keberlanjutan wilayah perbatasan Kabupaten Bengkayang dari lima dimensi keberlanjutan. Analisis menggunakan metode Multi-Domensional Scaling (MDS) yang disebut Rap-BENGKAWAN dan hasilnya dinyatakan dalam bentuk indeks dan status keberlanjutan. Untuk mengetahui atribut yang sensitif berpengaruh terhadap indeks dan status keberlanjutan dan pengaruh galat, dilakukan analisis Laverage dan Monte Carlo. Hasil analisis menunjukkan bahwa dimensi ekologi berada pada status kurang berkelanjutan (40,37%), dimensi ekonomi cukup berkelanjutan (66,54%), dimensi sosial-budaya cukup berkelanjutan (67,07%), dimensi infrastruktur dan teknologi tidak berkelanjutan (24,49%), dan dimensi hukum dan kelembagaan cukup berkelanjutan (60,10%). Dari 47 atribut yang dianalisis, 22 atribut yang perlu segera ditangani karena sensitif berpengaruh terhadap peningkatan indeks dan status keberlanjutan dengan tingkat galat (error) yang sangat kecil pada taraf kepercayaan 95%.
The Potencial of Bamboo Shoot for Health Yenni Okfrianti; Catur Herison; Fahrurrozi Fahrurrozi; Budiyanto Budiyanto
AGRITEPA: Jurnal Ilmu dan Teknologi Pertanian Vol 8 No 2 (2021)
Publisher : UNIVED Press, Universitas Dehasen Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37676/agritepa.v8i2.1471

Abstract

Bamboo is naturel mixture because it can survive in many habitats and belongs to the order of angiosperms monocots. There are around 1200 – 1500 species of bamboo in the world that grow in hilly areas but do not grow in alkaline, desert and rice fields. Bamboo shoots can be processed into food and even herbal and traditional medicines. Bamboo shoots are a sourece of fiber that can be used as a nutraceutical. Bamboo shoots contain as much as 2.23 – 4.20 gfiber in 100 wet weights in the form of flour, thin slices and capsules. Dendrocalamus asper bamboo shoot flour, Bambusa tuldoides and Bambusa vulgaris with air content < 10 g /100, protein, lipid and ash content< 3 g /100 g and can obtain mineral content > 60 g /100g. Bamboo shoots are a good source of dietary fiber. This review supports the increasing benefits of bamboo shoots in the supports the increasing benefit in the health world to prevent an increase in blood sugar, as antihypertention, and antihyperuresemia. Bamboo shoots can be processed into fermented products containing Lactid Acid Bacteria (BAL) which are beneficial for intestinal microflora which are recommended as probiotics.
KERAGAAN PERTUMBUHAN VEGETATIF DAN REPRODUKTIF HIBRIDA JAGUNG PERSILANGAN GALUR INBRIDA MUTAN (M4) PADA LATOSOL DARMAGA Rustikawati Rustikawati; Catur Herison; Surjono H. Sutjahjo
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 12, No 1 (2010)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.12.1.55-60

Abstract

[VEGETATIVE AND REPRODUCTIVE PERFORMANCES OF MAIZE HYBRIDS GENERATED FROM INBRED MUTANTS (M4) ON DARMAGA LATOSOL]. The development of hybrids from local germplasms are extensively done as alternative to the expensive imported hybrid seeds. The farthest genetic distance of parental lines is required to develop the best hybrids. The objective of this study was to compare the vegetative and reproductive performances of hybrids generated from crosses of M4 inbred lines, generated from the selected gamma irradiated mutants. Twenty eight hybrids derived from diallel crosses of M4 inbred lines and a check hybrid variety (NK33) were evaluated on Darmaga latosol. The results showed that all hybrids had lower performances on vegetative and reproductive characters compared to NK33. However, based on the selection index involving all characters, hybrids of G3 x G8, G6 x G8, and G8 x G6  with the index values of   9.45, 7.85, and 6.72, respectively,  performed better than or similar to  NK33.
Genetic Diversity Analysis in 27 Tomato Accessions Using Morphological and Molecular Markers Catur Herison; Surjono H. Sutjahjo; Ineu Sulastrini; Rustikawati Rustikawati; Siti Marwiyah
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i1.726

Abstract

Genetic diversity is the most important aspect in tomato breeding activities. Better assessment on the diversity of the collected accessions will come up with better result of the cultivar development. This study aimed at analyzing the genetic diversity of 27 tomato accessions by morphological and molecular markers. Twenty seven accessions collected from various regions of Indonesia were planted in the field and evaluated for their morphological traits, and RAPD analyzed for their molecular markers. The UPGMA clustering analyzes, elaborating the combination of morphological and molecular data, indicated that the tomato accessions could be grouped into 5 major groups with 70 % genetic similarity levels. Current study indicated that although many accessions came from different locations, they congregated into the same group. Cherry, Kudamati 1 and Lombok 3 were the farthest genetic distant accessions to the others. Those three genotypes will be the most valuable accessions, when they were crossed with other accessions, for designing a prospective breeding program in the future.
Effect of BAP (6-Benzyl Aminopurine) on In Vitro Shoot Growth of Curcumas Rustikawati Rustikawati; Catur Herison; Entang Inoriah; Vera Dwisari
AGRITROPICA : Journal of Agricultural Sciences Vol 4, No 1 (2021)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.4.1.82-92

Abstract

Curcuma sp has been widely investigated for its anti-cancer properties. Conventionally, vegetative propagation needs a long time to produce a large number of planting materials, so that it is necessary to find an alternative approach through in vitro propagation.  The effect of BAP on the in vitro shoot formation of ‘temu putih’ and ‘temu putih’ has been investigated in this study. The experiment was a 4x2 factorial with 5 replications arranged in a completely randomized design.  The first factor was the concentration of BAP i.e. 0, 1.5, 3, and 4.5 ppm.  The second factor was the curcuma species consisting of ‘temu putih’ (Curcuma zedoaria Roch.) and ‘temu mangga’ (Curcuma mangga Val.).  Each experimental unit consisted of 2 in vitro bottles, each of which planted with 1 explant bud.  Analysis of variance was conducted on percentage of live explants, shoot height, number of roots, root length, wet weight, percentage of explants that sprouted, percentage of rooted explants and shoot color.  Mean comparison was performed by the Least Significant Difference (LSD).  The results showed that there was no interaction between BAP concentration and genotype on any variable observed.  The shoot growth of ‘temu putih’ was significantly higher than ‘temu mangga’ in vitro.  The best concentration of BAP for the growth of ‘temu putih’ and ‘temu mangga’ shoots was 1.5 ppm.
Response of 25 Hybrid Maize Against Salinity Stress and Their Performance in Coastal Area Meko Gustian; Rustikawati Rustikawati; Widodo Widodo; Catur Herison
AGRITROPICA : Journal of Agricultural Sciences Vol 2, No 2 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (958.619 KB) | DOI: 10.31186/j.agritropica.2.2.56-70

Abstract

Plant stress due to NaCl compound will have an impact on the reduction of plant growth and yield.  Tolerant varieties are required  to increase plant production in coastal areas since these areas are closely related to salinity.  This study aimed to (1) determine the appropriate NaCl concentration for saline tolerance in maize stress selection, (2) determine the response of maize plant to salinity stress in nutrient culture, and (3) determine the response of new hybrid maize having adaptibility in coastal area.  Two experiments were run from November 2017 until May 2018. The first experiment was conducted in a greenhouse at the Laboratory of Agronomy, Faculty of Agriculture University of Bengkulu  to determine the tolerance response of 25 new hybrid maize to salinity in nutrient culture.  The second experiment was conducted on the coastal areas in Beringin Raya Village, Muarabangkahulu District, Bengkulu City to determine the response of new hybrid maize having adaptibility in a coastal areas.  The concentration of  NaCl at which level the LC50 was determined was found to be in 150 mM. This concentration was then used to test the tolerance of 25 new hybrid maize to NaCl stress.  The hybrids of CT18, CT19, CT22, CT25, CT31, CT33, CT34, CT40, CT47, and  CT50 showed good responses to the concentration treatment  of 150 mM NaCl based on plant fresh weight and shoot dry weight. The hybrids of CT17,CT19, and CT20 showed good adaptibility on coastal area based on the length of cob, diameter of cob, seed weight per cob, and cob weight per plant.  Among those three hybrids, CT17 and CT20 resulted low fresh weight and low shoot dry weight.  The factors other than NaCl in coastal area maybe affect better response. However, CT19 proved as a hybrid which was consistently in a good response to salinity stress both in nutrient culture and in coastal growing area.
Evaluasi kinerja dan status keberlanjutan kawasan Agropolitan Perpat Belitung Hariyadi Hariyadi; Catur Herison; Edi Suwito
Agrikan: Jurnal Agribisnis Perikanan Vol 5, No 1 (2012)
Publisher : Sangia Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.agrikan.5.1.24-30

Abstract

Penelitian dilakukan pada kawasan agropolitan Perpat Belitung, selama bulan November 2009 sampai dengan bulan Maret 2010 dengan metode studi literatur, survey lapangan, wawancara dan questioner dan Analisis-analisis yang digunakan antara lain analisis Location Quetient (LQ), analisis unggulan dan andalan, metode pendekatan Multi Dimensional Scaling (MDS) Rap-Agrop, analisis Laverege,  analisis Monte Carlo dan analisis Prospektif serta analisis Deskriptif, yang bertujuan untuk : mengetahui potensi kawasan dalam mendukung pengembangan agropolitan Perpat,) mengevaluasi tingkat kinerja perkembangan kawasan agropolitan Perpat, mengevaluasi status keberlanjutan pengembangan kawasan agropolitan Perpat, mengetahui persepsi dan kebutuhan masyarakat terhadap agropolitan. Hasil penelitian menunjukan bahwa masing-masing kecamatan memiliki dominansi komoditas yang beragam diusahakan dan dibudidayakan dan terdapat lima (5) komoditas unggulan yaitu Padi, Ubi Kayu, Lada, Kelapa dan komoditas Sapi Potong yang ditetapkan sebagai komoditas unggulan utama program agropolitan, dengan tingkat kinerja perkembangan wilayah termasuk dalam Strata PRA II Kawasan Agropolitan yang berstatus keberlanjutan pengembangan Kawasan Agropolitan Perpat termasuk dalam Strata kurang berkelanjutan, dan penetapan Kawasan Agropolitan Perpat dengan komoditas unggulan utama sapi potong tidak tepat untuk dikembangkan oleh karena kondisi existing tingkat perkembangan yang rendah dan status keberlanjutan yang kurang keberlanjutan.
Peningkatan Pengetahuan Dan Ketrampilan Kelompok Tani Melalui Penerapan Paket Teknologi Tepat Guna Produksi Cabai Merah Di Lahan Masam Catur Herison; Rustikawati Rustikawati; Edhi Turmudi; Parwito Parwito; Edi Susilo
DHARMA RAFLESIA Vol 19, No 1 (2021): JUNI (ACCREDITED SINTA 5)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/dr.v19i1.15696

Abstract

Petani pengelola lahan masam umumnya mengalami persoalan tidak mencu-kupinya hasil yang diperoleh karena produk-tivitas lahannya rendah.  Keinginan petani untuk meningkatkan pendapatan melalui budidaya tanaman yang bernilai ekonomi tinggi,khususnya cabai merah terkendala keterbatasan pengetahuan dan ketrampilan dalam budidaya secara intensif.  Kegiatan Pengabdian pada Masyarakat (PPM) ini bertujuan melakukan hilirisasi hasil penelitian kelompok peneliti Universitas Bengkulu dalam bentuk Paket Teknologi produksi cabai merah untuk membantu mengatasi permasalahan rendahnya produktivitas tanaman di lahan masam melalui peningkatan pengetahuan dan ketrampilan kelompok tani.  Kegiatan PPM dilakukan dengan metode pelatihan berupa penyuluhan, praktek dan demonstrasi plot, di Kelompok Tani Bangun Jaya, Kelurahan Bentiring, Kecamatan Muara Bangkahulu, Kota Bengkulu, dan Kelompok Tani Melati Mekar Bersama, Desa Pekik Nyaring, Kecamatan Pondok Kelapa, Kabupaten Bengkulu Tengah.  Berdasarkan evaluasi yang dilakukan, pelatihan paket teknologi budidaya cabai di ultisol dapat meningkatkan pengetahuan petani dalam pengelolaan lahan masam dan teknik budidaya cabai merah intensif. Metode penyuluhan dan demonstrasi plot efektif untuk menyampaikan tujuan kegiatan pengabdian pada masyarakat.
Co-Authors A.A. Ketut Agung Cahyawan W Adeputri, Eriana Agus Purwito Almunawar, Said Agil Alnopri Andria, Andria Angraini, Dila Anira, Rayuli Arifin Noor Sugiharto Arifin Noor Sugiharto Atang Sutandi Atra Romeida Atra Romeida Bertham, Yudhy H Budianto Budianto Budianto Budianto Budiyanto Budiyanto Dewi, Kurnia Herlina Dwi Wahyuni Ganefianti, Dwi Wahyuni Dwitama, Niko Edhi Turmudi EDHI TURMUDI Edi Susilo Edi Suwito Eko Suprijono Eko Suprijono Emilda Tri Mauli Entang Inoriah Fahrurrozi Fahrurrozi Fahrurrozi, Fahrurrozi Hairani, Putri Mian Hariyadi Hariyadi Hasanudin Hasanudin Hasanudin Hellianti Pennita Herlinda, Puput HESTI PUJIwATI Hutabarat, Grace May Sawita Indres, Indres Ineu Sulastrini Joko Suharjo, Usman Kris Laksono Trisnantoro Maemunah Maemunah Marulak Simarmata Marulak Simarmata Marulak Simarmata Marwanto Marwanto Meko Gustian Merakati Handajaningsih Merakati Handajaningsih Merakati Handajaningsih Merakati Handayaningsih Merakati Handayaningsih Mimi Sutrawati Muhammad Chozin, Muhammad Mukhtasar, Mukhtasar Nabila, Athya Kania Nadia, Nur Ain nFN Thamrin Noor Farid Novrika, Donda Nurjanah, Uswatun Nursalim Nursalim Nurwita Dewi Okticah, Anggi Parwito Parwito Reny Herawati Ria Meita Rustikawati Rustikawati Rustikawati Rustikawati Rustikawati Saputra, Pringky Okta Sari, Miliza Mayang Sari, Rossi Anandayu Sianipar, Mangara W Sigit Sudjatmiko, Sigit Simanihuruk, Bilman Wilman Sri Winarsih Sudarsono Suharjo, Usman K.J. Suharjo, Usman Kris Joko Sukisno Sukisno Sukisno Sukisno, Sukisno Sulastrini, Ineu Surjono H. Sutjahjo Surjono H. Sutjahjo Surjono H. Sutjahjo Surjono H. Sutjahjo Surjono H. Sutjahjo Surjono Hadi Sutjahjo Surmaini, Ervi Suryati, Dotti Sutjahjo, Surjono H. Suyitman Suyitman Suyitman, Suyitman Syakia Kurin, Aricha Thamrin, nFN Tunjung Pamekas, Tunjung Vera Dwisari Viona Juanda Putri Widodo Widodo Wuri Prameswari Yenni Okfrianti Yulian, Yulian Zainal Muktamar