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All Journal Biospecies Bioscience Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Agrotek Tropika JURNAL PENDIDIKAN TAMBUSAI Eksakta : Berkala Ilmiah Bidang MIPA Atrium Pendidikan Biologi Serambi Biologi MANAZHIM Pelita Eksakta Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi Jurnal Biosense Scientific Journal Jurnal Embrio Sabangka Abdimas Jurnal Pengabdian Masyarakat Sabangka Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi Jurnal Sains dan Kesehatan Edumaspul: Jurnal Pendidikan
Moralita Chatri
Universitas Negeri Padang
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Test of the Ability of Fluorescent Pseudomonas to Dissolve Phosphate Norman, Mayanda; Advinda, Linda; Chatri, Moralita; Handayani, Dezi
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.11143

Abstract

Phosphate is a vital nutrient required for plants, yet its presence in the soil is frequently limited due to being held by specific ions. Microorganisms that solubilize phosphate, particularly fluorescent Pseudomonas, are considered among the most efficient agents for phosphate solubilization. This research intends to assess the capacity of six fluorescent Pseudomonas isolates derived from the rhizosphere of different plants to solubilize phosphate, and to analyze their IKF values. The research was carried out descriptively from June to August 2025 at the Plant Physiology Laboratory, Faculty of Mathematics and Natural Sciences, Padang State University. Observations were conducted by noticing the development of clear zones and determining the IKF values. The findings indicated that every isolate, specifically PfPj1, PfS36, PfLAHP2, PfCas, PfS31, and PfKd7, was capable of solubilizing phosphate. The highest IKF value was found in isolate PfKd7 (4.91), whereas the lowest was recorded for PfS36 (4.08). Variations in IKF values indicate differences in the capacity of each isolate to release organic acids, which are involved in the phosphate solubilization process. This discovery reinforces the capability of fluorescent Pseudomonas as an eco-friendly biological agent to enhance phosphorus availability.
UJI EFEKTIVITAS FUNGISIDA KIMIA TERHADAP JAMUR PATOGEN Fusarium sp. SECARA IN VITRO Insyira, Cleoraissa; Alhusaeri Siregar, Bayo; Chatri, Moralita
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8001

Abstract

Fusarium sp. is a pathogenic fungus causing wilt disease in Acacia crassicarpa nurseries and reducing seedling quality in industrial forest plantations. The control of this disease is generally done with chemical (synthetic) fungicides, but the most effective one is not yet known. This study aimed to evaluate the effectiveness of several chemical fungicides against Fusarium sp. growth in vitro. The study was conducted from February to June 2025 at the Plant Protection Department (PPD) Laboratory, R&D Division of PT. Arara Abadi using a Completely Randomized Design with four treatments: control, Octave (prochloraz–manganese chloride complex), Besromil (metalaxyl), and Odeon (chlorothalonil), with three replications. The observed parameter was the percentage of fungal growth inhibition. Data were analyzed using ANOVA followed by Duncan’s test at a 5% significance level. The results showed that Octave exhibited the highest inhibitory effect with 100% inhibition and was significantly different from other treatments. Besromil and Odeon showed low inhibition and were not significantly different from the control. Therefore, prochloraz–manganese chloride complex was the most effective fungicide in inhibiting Fusarium sp. growth in vitro.
KEMAMPUAN PSEUDOMONAS BERFLUORESEN SEBAGAI AGEN ANTAGONIS TERHADAP Ralstonia solanaceaerum PENYEBAB LAYU TANAMAN NILAM (Pogestemon cablin Benth) Pratama Putra, Abil; Advinda, Linda; Chatri, Moralita; Handayani, Dezi
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8088

Abstract

Patchouli (Pogestemon cablin Benth) is a cultivated plant that can produce oil and generate income for farmers. However, attacks by plant pests (OPT) often pose a challenge to patchouli production in Indonesia. One organism that causes wilt disease in patchouli plants is the bacterium Ralstonia solanaceaerum. Therefore, more environmentally friendly and sustainable control methods are needed. One strategy is the use of biological agents, especially antagonistic rhizosphere bacteria such as fluorescent pseudomonas. This study was conducted from June to August 2025 at the Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This study used a completely randomized design (CRD) as the experimental design. In this study, there were 6 treatments (different isolates), each repeated 3 times. The parameter observed was the formation of an inhibition zone using the disc diffusion method. The data were analyzed using analysis of variance (ANOVA). If there were significant differences, Duncan's New Multiple Range Test (DNMRT) was used at a 5% level. Based on the results and discussions, it can be concluded that all fluorescent Pseudomonas isolates tested have the ability to inhibit R. solanaceaerum, the cause of wilt disease in patchouli plants. The isolate with the best ability to inhibit the growth of R. solanaceaerum on patchouli plants was isolate Pf S36 with an inhibition zone of 20.37 mm, but there was no significant difference between the other isolates because the inhibition effectiveness was relatively uniform.
KEANEKARAGAMAN TUMBUHAN ANGIOSPERMAE DI LINGKUNGAN KAMPUS FMIPA UNIVERSITAS NEGERI PADANG Khairani, Annisa; Chatri, Moralita
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8092

Abstract

Angiosperms are a group of closed seed plants that have an important role in maintaining the balance of the ecosystem, especially in the campus environment that functions as a green open space. This study aims to know the diversity of Angiosperms found in the Faculty of Mathematics and Natural Sciences (FMIPA) Campus environment, Padang State University (UNP). The method used is direct observation with exploration techniques in several locations of the FMIPA campus. The data collected include local names, scientific names, families, and classifications of Angiosperms. The results of the observations show that the FMIPA UNP Campus environment has a fairly high diversity of Angiosperms, consisting of various monocot and dicot species originating from various families. This diversity is influenced by environmental conditions, management of campus green spaces, and the use of plants as ornamental plants, shade plants, and medicinal plants. The presence of Angiosperms in the campus environment plays an important role in supporting ecological, aesthetic, and educational functions.
KEANEKARAGAMAN TUMBUHAN DIKOTIL DI KAMPUS UNIVERSITAS NEGERI PADANG Domisofa, Melani; Chatri, Moralita
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8093

Abstract

This study was motivated by the important role of campus green spaces in supporting plant biodiversity conservation. The aim of this research was to identify and describe the diversity of dicotyledonous plants on the campus of Universitas Negeri Padang, West Sumatra, Indonesia. The research was conducted from November to December 2025 using a descriptive method. Data were collected through direct field observations by recording morphological characteristics of dicotyledonous plants, including growth habit, stems, leaves, and flowers. Plant identification was supported by the PlantNet application and relevant botanical literature. The data were analyzed descriptively by classifying species based on taxonomic levels. The results showed that all recorded plants belonged to the dicotyledonous group within the class Magnoliopsida, comprising 25 species, 22 genera, 14 families, and 10 orders. Dominant families included Anacardiaceae, Myrtaceae, Euphorbiaceae, Rubiaceae, Apocynaceae, Solanaceae, and Combretaceae, which exhibit diverse ecological roles and benefits. In conclusion, the Universitas Negeri Padang campus supports a relatively high diversity of dicotyledonous plants and functions as an important habitat for plant biodiversity. This study is recommended as baseline data for green space management, further botanical research, and biology education.
ANALISIS KONTAMINASI JAMUR PADA KUNYIT GILING CURAH DI PASAR LUBUK BUAYA KOTA PADANG Azzahra, Zulia Putri; Handayani, Dezi; Chatri, Moralita; Irdawati
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8185

Abstract

Keamanan pangan sangat penting untuk melindungi kesehatan masyarakat, khususnya untuk produk pangan yang rentan terhadap kontaminasi mikroba. Kunyit bubuk curah yang dijual di pasar tradisional berisiko terkontaminasi jamur karena praktik penanganan yang tidak higienis, yang dapat mengurangi kualitas produk dan menimbulkan bahaya kesehatan. Penelitian ini bertujuan untuk mengetahui tingkat kontaminasi jamur pada kunyit bubuk curah yang dijual di Pasar Lubuk Buaya, Kota Padang, dan untuk mengidentifikasi karakteristik makroskopis jamur yang tumbuh. Penelitian ini dilakukan dari Oktober 2025 hingga Januari 2026 di Laboratorium Mikrobiologi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang. Desain penelitian deskriptif digunakan dengan metode pengambilan sampel purposif. Sampel kunyit bubuk curah dipilih dan dikumpulkan dari lima pedagang dengan tingkat kebersihan yang berbeda. Kontaminasi jamur dinilai menggunakan metode penghitungan kapang dan ragi (MYC) dengan teknik spread plate pada media Potato Dextrose Agar (PDA), diikuti dengan pengamatan makroskopis morfologi koloni jamur. Hasil menunjukkan bahwa semua sampel kunyit bubuk curah terkontaminasi jamur, dengan tingkat kontaminasi berkisar antara 2,67 × 10⁵ hingga 2,9 × 10⁶ CFU/g. Semua sampel melebihi batas maksimum yang diizinkan untuk kontaminasi mikroba sebagaimana ditetapkan oleh Badan Pengawas Obat dan Makanan Indonesia (BPOM). Jamur kontaminan yang diidentifikasi meliputi Aspergillus flavus, A. niger, Penicillium sp., dan ragi. Oleh karena itu, dapat disimpulkan bahwa kunyit bubuk curah yang dijual di Pasar Lubuk Buaya, Kota Padang, tidak memenuhi standar keamanan pangan mikrobiologis.
POTENSI ISOLAT Pseudomonas BERFLUORESEN DALAM MENGHASILKAN ASAM SIANIDA (HCN) UNTUK APLIKASI AGENS HAYATI aulia, fadila; Advinda, Linda; Chatri, Moralita; Farma, Siska Alicia
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.6804

Abstract

Penelitian ini bertujuan untuk menilai kemampuan beberapa isolat Pseudomonas berfluoresen dalam menghasilkan asam sianida (HCN) sebagai indikator potensi agens hayati. Lima isolat diuji, yaitu Pf Cas 3, Pf Cas, Pf LAH T2, Pf LAH, dan Pf LAH S1, serta satu kontrol tanpa inokulum. Pengujian dilakukan menggunakan indikator asam pikrat–Na₂CO₃ dan pengukuran nilai absorbansi dengan spektrofotometer pada panjang gelombang 625 nm. Hasil menunjukkan bahwa isolat Pf Cas 3, Pf Cas, dan Pf LAH T2 menghasilkan HCN dengan nilai absorbansi masing-masing 0,021; 0,014; dan 0,012. Sementara Pf S32 dan Pf LAH S1 menunjukkan hasil negatif dengan nilai absorbansi rendah (0,003 dan 0,002). Kontrol menunjukkan nilai 0,00. Isolat Pf Cas 3 dikategorikan paling potensial untuk dikembangkan sebagai agens hayati penghasil HCN dalam mendukung sistem pertanian berkelanjutan.
UJI ANTAGONIS BAKTERI ENDOFIT Pseudomonas BERFLUORESEN TERHADAP RALSTONIA SOLANACEARUM PENYEBAB LAYU TANAMAN TOMAT DAN NILAM Nazri, Laila Mardhiyah; Putri, Dwi Hilda; Advinda, Linda; Nuraini, Fauzi Eka; Handayani, Dezi; Chatri, Moralita
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.6876

Abstract

Penyakit layu bakteri yang disebabkan oleh Ralstonia solanacearum merupakan salah satu kendala utama dalam budidaya tomat dan nilam karena mampu menurunkan produktivitas secara signifikan. Penggunaan agen hayati seperti pseudomonas berfluoresen menjadi alternatif pengendalian yang lebih aman dan ramah lingkungan. Penelitian ini dilakukan untuk mengetahui kemampuan antagonis tujuh isolat bakteri endofit pseudomonas berfluoresen yang berasal dari akar pisang buai dalam menghambat pertumbuhan R. solanacearum yang menginfeksi tomat dan nilam. Penelitian memakai pendekatan deskriptif-kuantitatif dan dilaksanakan di Laboratorium Biologi FMIPA UNP pada Agustus–November 2025. Isolat pseudomonas berfluoresen dan R. solanacearum diremajakan pada media selektif, kemudian diuji antagonismenya menggunakan metode swab pada medium MHA. Suspensi patogen distandarkan pada McFarland 0,5, sedangkan isolat antagonis diinokulasikan secara titik. Zona hambat yang terbentuk setelah inkubasi 24 jam diukur menggunakan jangka sorong dan dianalisis secara deskriptif. Hasil penelitian menunjukkan adanya variasi kemampuan penghambatan antar isolat. Pada patogen tomat, isolat PfB305, PfB309, PfB311, dan PfB322 menghasilkan zona hambat yang relatif besar, sedangkan PfB32, PfB51, dan PfB52 tidak menunjukkan aktivitas antagonis. Pada patogen nilam, tiga isolat yang sebelumnya tidak aktif pada patogen tomat justru menghasilkan zona hambat, sementara empat isolat lainnya tidak membentuk zona hambat. Penelitian ini menunjukkan bahwa efektivitas pseudomonas berfluoresen dipengaruhi oleh spesifisitas isolat terhadap patogen serta potensi produksi metabolit antibakteri
The Influence of the STEM-Based Problem-Based Learning Model on Students' Science Literacy Skills in Science Learning Zaharani, Arum; Syamsurizal; Fadilah, Muhyiatul; Chatri, Moralita
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.13435

Abstract

This research is motivated by the low level of science literacy among students in Indonesia, including at SMP Negeri 17 Kota Jambi. The study aims to determine the effect of the STEM-based PBL model on the science literacy of seventh-grade students at SMP Negeri 17 Kota Jambi. This type of research is a quasi-experiment with a pre-test post-test control group design. The research sample consisted of two classes: the experimental class (VII C) with 32 students who received the STEM-based PBL model treatment, and the control class (VII E) with 31 students who used the direct instruction model.  The research results science literacy skills improved, with an average pre-test score of 58.5 and a post-test score of 79.75, while the control class increased from 38.32 to 60.38. The N-Gain value for science literacy in the experimental class was 50.83 (moderate category), which was higher than the control class's 38.32. The t-test results show a significance value of 0.037 < 0.05, which means there is a significant difference between the two groups. It can be concluded that the implementation of the STEM-based PBL model is effective in improving students' science literacy skills in science learning.
Etnobotani Tumbuhan Obat di Nagari Mungka Kecamatan Mungka Kabupaten Lima Puluh Kota Farras, Fadhila Annisa; Putri, Dwi Hilda; Chatri , Moralita; Handayani , Dezi
Scientific Journal Vol. 5 No. 2 (2026): SCIENA Volume V No 2, March 2026
Publisher : CV. AKBAR PUTRA MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56260/sciena.v5i2.318

Abstract

Pendahuluan: Tumbuhan Obat merupakan tumbuhan yang digunakan sebagai bahan baku pengobatan dalam mengatasi penyakit. Tujuan penelitian: Mengetahui jenis-jenis tumbuhan obat, organ tumbuhan yang digunakan serta khasiat dan cara penggunaannya di Nagari Mungka. Metode : Jenis penelitian ini adalah deskriptif eksploratif. Sampel penelitian ini berjumlah 23 responden. Analisis data secara kualitatif dengan dukungan pustaka ilmiah, kemudian data yang diperoleh disajikan dalam bentuk tabel. Hasil : ditemukan 39 jenis tumbuhan obat yang terdiri dari 29 familia. Tumbuhan obat yang banyak ditemukan yaitu familia Euphorbiaceae, Fabaceae, Lamiaceae masing-masing sebanyak 3 species, Organ tumbuhan yang banyak digunakan adalah daun. Cara pengolahan tumbuhan obat yang digunakan dengan cara direbus, dibakar, digiling atau dihaluskan, dihancurkan, dan diremas. Sedangkan untuk pemanfaatan tumbuhan obat ada yang diminum, diurut, diusap, dioles, dtempel, digosok dan dikonsumsi langsung. Kesimpulan : Masyarakat di Nagari Mungka masih memanfaatkan tumbuhan sebagai obat tradisional.
Co-Authors . Mansyurdin Abdul Razak Abdul Razak Achyar, Afifatul Afdarina, Oksa Afifa Akhyar Ahmad Subari Alhusaeri Siregar, Bayo Amalia, Irna Amri Bakhtiar Annisa Tulhasanah Arlina, Sistika Atifah, Yusni Aulia, Fadila Auliani Arafah Ayunda Intan Kartika Az – Zahra Nabillah Azizah, Salum Azwir Anhar Azzahra, Zakila Azzahra, Zulia Putri Beni Gusnadi Billa Firdaus Tanjung, Shalsa cynthia perdana putri Des M Des M Des M Des Des M. Des M. M. Dezi Handayani Dian Intan Rahmadhni Dilla, Aprina Dina Sukma Dinda Alde Rahmadhani Rahmadhani Domisofa, Melani Dr. Des M.S Dr. Irdawati M. Si Dwi Hilda Putri Edwin Edwin Eldyana Rahayu Putri Elsa Yuniarti Eria Marina Sepriyani Erwina Simanullang fadia salsabil arsy FAJRINA, SUCI Farras, Fadhila Annisa Febriani Dika Suryendra Fevria, Resti Ficil Mikaf Filza Yulina Ade Fransisco, Sandi Fronica, Imelda Ghiffari, Muhammad Handayani , Dezi Hani Fitriani Heffi Alberida Helendra Helsa Rahmatika Herina, Nandia Idos Susila Ningsih Ilfia Islamiati Putri Imelda Nasrul, Putri Insyira, Cleoraissa IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati M.Si Irdawati Zalni Irma Leilani Eka Putri Jumjunidang Jumjunidang Kardiman, Reki Kartika Sari Khairani, Annisa Khotimah, Anggun Khumaira, Kayla Anaya Kurnia Illahi, Rani Kurnia Wulansari Larashinda, Mentari Latif, Zalik Muhammad Linda Advinda Linda Advinda Lufri Lufri Mades Fifendy Mantoviana, Tiffany Mardiana Ikhwan Marisa Marisa Marsha Utami Mayarlis Mei Niarti Meriza Fatma Mesy Maisarah Miftahul Jannah Milka Saputri Monica , Indiastri P Muhardini, Mike Muhyiatul Fadilah Muhyiatul Fadilah Mukhlis Mukhlis N.Sri Hartati Nabilah, Rezi Nadia Naldi, Henra Nanang Taryana Natasya Hersila Nazhifah, Naura Nazri, Laila Mardhiyah Niken Maharani Putri Norman, Mayanda Nuraini, Fauzi Eka Nurlita, Dwi Olan Tri Sinaga Perri Adnadi Pratama Putra, Abil Pratama, Sandi Fransisco Puspa Anggraeni Putri Putri Widianti Putri, Amaliani Putri, Aulia Devani Putri, Irma Leilani Putri, Irma Leilani Eka Putri, Ulfa Dwi Rahmawati Darussyamsu Rahmi Holinesti Rahmi, Elva Relsas Yogica, Relsas Resti Yulia Riana Aftafia Rizka Putri Alti Roza, Sri Yenica Rusendra, Nabilla Makra Sabrina Mandai, Della Saputra, M. Rizki Sari Yulianti Selaras, Ganda Hijrah Sholichin, Muhamad Silvi Lathifah SRI RAHAYU Sri Widyaningsih Suci Fajrina Suci Fajrina Syahrastani, Syahrastani Syamsurizal Syamsurizal - - Syamsurizal Syamsurizal Syamsurizal Syamsurizal Tita Yunia Zalni Tomi Apra Santosa Santosa Vauzia Vauzia M.Si Vauzia Vauzia Vauzia, Vauzia Vauzia, Vauzia Via Susana Gusni Violita Violita Violita Violita Violita Violita Violita Viona, Alda Wawan Sujarwo Wulansari, Kurnia Yanti Srinita Utami Yesti Yesti Yosi Safritry Yulanda Saputri Zaharani, Arum Zahratul Aini