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Journal : JURNAL BIOLOGI INDONESIA

Pengaruh Radiasi Sinar Gamma dan Asam Fusarat untuk Meningkatkan Ketahanan Abaka (Musa textilis Nee) terhadap Fasariurn oxysporum Damayanti, Fitri; Suharsono, Suharsono; Mariska, Ika
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (707.082 KB) | DOI: 10.14203/jbi.v3i4.3285

Abstract

ABSTRACTEffect of gamma radiation and fusaric acid for resistance to wild Fusarium disease onabaca plant (Mum textillis Nee). The problem in abaca production is wilt disease infectioncaused by Fusarium oxysporzim. The resistant variety against the pathogen has not beenavailable yet. The disease resistance character of the species might be improved throughsomaclonal variation and in vitro selection. Different pure toxin of fusaric acid concentration(0, 15,30,45,60, and 75 mg/l) was used as component selection to get new hope numbers ofresistant abaca to wilt Fusarium disease through in vitro selection. Concentration of 45 mgllfusaric acid is lethal for abaca, so we used this concentration as dose of selection to selectshoots from irradiated calli. Gamma irradiation was used as mutagent to increase somaclonalvariation on abaca. Six levels of gamma-ray radiation (0, 0.5, 1, 2, and 3 Krad) were appliedto embriogenic calli. Increasing dose of radiation decreased the viability of calli. LDso wasfound between 1-1.5 Krad of radiation dose. In vitro selection was carried out in two stages.The concentration of selection of hsaric acid in stage I1 was increased one level to theconcentration in stage 1. Stage I selection of shoots from irradiated calli on mediumcontaining pure toxin 45 mg/l fusaric acid, showed that the survival capacity decreasing withthe increasing doses of gamma irradiation. In stage 11, shoots from irradiated calli (at 0.5 and1 Krad) could survive on medium containing 60 mg/l fusaric acid. In medium selectioncontaining 50% filtrate F. oxysporum, fusaric acid resistant shoots were also filtrate resistant.There was a correlation between in vitro fusaric acid and filtrate of F. oxysporum resistantplant and conidia suspension of F. oxysporum resistkt plant in the greenhouse.Keywords: Gamma radiation, in vitro selection, fusaric acid, Musa textilis, Fusariumoxysporum
Co-Authors Aan Risdiana Abdilqoyyim, Fanky Abdul Rahim Acep Musliman Adawiyah, Asyifa Robiatul Alfarez, Faruq Anas Ansoriah Andri Indrawan Apriliana, Teguh Firmansyah Ariadi, Deni Arik Kurniawati Arrova Dewi, Deshinta A’ini, Zakiyah Fithah Christiana, Zubaidah Dafa Fahrezi, Nafian Dessy Herlisnawati Dewi Taurusyanti Doni Abdul Fatah Efri Gresinta Fauziah, Farrah Hany Firdaus Firdaus Fitri Minawati Gilang Patria, Asta Giry Marhento Haryani, Rini Hasbullah Hasbullah Hayatunnufus, Gina Hymawati, Hymawati Idang Nurodin Ika Mariska Ika Roostika Jibran, Mohammad Fadel Jupriadi, Jupriadi Kalyana, Dyani Khoiroh, Nisaul Kristiningrum, Kristiningrum Kusharisupeni Kusharisupeni Lana Zulkarnain, Ratna Larashati, Larashati Lintang Ratri Rahmiaji Marda’is Margaretta Serevina Hutagalung Mashitapasha, Revica Maspupah Mohammad Syarief, Mohammad Naufal Azmi Verdikha Noer, Shafa Nuraeni, Dede Eni Nurul Aisyah Nurul Isnaeni Ochtora, Danesty Oktavia Suzanti, Ika Permana, Yohan Pramana Syah Putra, Arlyan Prasetyo, Nanda David Pratiwi, Rosa Dewi Priyonggo Purba, Christina Purnawan, I Ketut Adi Putra, Erik Perdana Safitri, Vivi Hariyanti Samsurianto Samsurianto Satria Erlangga, Bima Sefudin, Akhmad Setyo B, Gideon Simanullang, Enjel Triastuti Siti Nurjanah Siti Nurul Qomariyah Sri Susanti Sri Trisnaningsih SUDARMAJI SUDARMAJI Sudrajat, Mahdi Suharno Suharno Suharsono Suharsono Sulis Supartini Sumarmia, Sumarmia Suprapto , Yoyon Kusnendar Supriyatin, Titin Tarwana, Wawan Taufik Jatnika Permana Titiek Hendriama, Titiek Tridahus Susanto, Tridahus Tridata, Aldrin Triyono Lukmantoro Ulyat, Ulyatun Fatonah Utami, Cintara Prili Widya Vivian Salsa Bila, Anandita Wahyudi Setiawan Wahyudi, Muhamad Machrus Ali Wibowo, Kukuh Cokro Wijaya, Hari Rachmat Yeni, Dede Yudi Nugraha Bahar Yuniarno , Eko Mulyanto Zania, Firda