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All Journal Bioscience Pendidikan Biologi Biocelebes Jurnal Penelitian Pendidikan IPA (JPPIPA) Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Biologi Tropis Jurnal Agrotek Tropika JURNAL PENDIDIKAN TAMBUSAI FONDATIA AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Atrium Pendidikan Biologi Serambi Biologi Pelita Eksakta Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan Sainstek : Jurnal Sains dan Teknologi Jurnal Abdi Insani Journal On Teacher Education (Jote) Jurnal Biosense Al Jahiz: Journal of Biology Education Research Tsaqofah: Jurnal Penelitian Guru Indonesia MASALIQ: Jurnal Pendidikan dan Sains Jurnal Serambi Biologi JRTI (Jurnal Riset Tindakan Indonesia)
Irdawati Irdawati
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Effect of the Thermophilic Bacterial Biculture Consortium from Mudiak Sapan Hot Springs on Biofuel Production Irdawati Irdawati; Fahra Fahra; Dwi Hilda Putri; Dezi Handayani; Yusrizal Yusrizal
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.3597

Abstract

Biofuels are fuels derived from biomass and consist of biogas, biodiesel and bioethanol. Bioethanol is a biofuel whose main ingredients are from plants and generally use microorganisms in the fermentation process. One way is to use thermophilic bacteria with the advantage of low contamination levels and better product quality. Bacteria that exist in nature are not only in a single form but also exist in the form of a consortium in which there are beneficial or detrimental bacterial interactions. Favorable bacterial interactions indicate that the bacteria are compatible. Using a consortium with compatible bacteria gives better results than using a single bacteria. This study aims to determine the compatibility and effect of a consortium of thermophilic bacteria from Mudiak Sapan hot springs on biofuel yields. This research is a descriptive research. To test the cooperation between isolates, a compatibility test was carried out using the disk diffusion method. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%.
The Validity and Practicality of SSCS-Based Student Worksheet on Ecology Material and Environmental Change Syamsurizal; Irdawati; Violita; Refni Syahleli Afni; Muhamad Sholichin
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4444

Abstract

This research aims to produce student worksheet based on SSCS (Search, Solve, Create and Share). This type of research is development research using the Plomp model. The development stage of this model starts from the initial investigation stage, the development or prototyping stage, and the assessment stage. The instruments used in this development research were teacher questionnaire sheets, student response questionnaire sheets, self-evaluation sheets, and SSCS-based LKPD validation sheets (Search, Solve, Create and Share) by experts, as well as SSCS-based LKPD practicality assessment sheets (Search, Solve, Create and Share) which is filled in by teachers and students. The results showed that the SSCS-based LKPD (Search, Solve, Create and Share) was very valid with a score of 86.31%. The practicality assessment of student assessment in field trials (group evaluation) with a score of 92.00% with very practical criteria, and the practicality assessment by the teacher obtained a score of 96.00% with very practical criteria. It can be concluded that the LKPD based on SSCS (Search, Solve, Create and Share) that has been developed is included in the category of very valid and very practical.
Pengaruh Model Pembelajaran Kooperatif Two Stay Two Stray (TSTS) Berbantuan Media Interaktif Berbasis STEM Terhadap Berpikir Kritis dan Hasil Belajar Siswa Pada Materi Sistem Indera Di SMA Negeri 2 Kerinci Eria Marina Sepriyani; Lufri; Irdawati; Yuni Ahda
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4551

Abstract

This study aims to determine the effect of the two-stay two stray cooperative learning model (TSTS) assisted by STEM-based interactive media on critical thinking and student learning outcomes on sensory system material at SMA Negeri 2 Kerinci. This type of research is quasi-experimental research with a randomized control-group posttest-only design. The population of this study came from class XI students of SMA Negeri 2 Kerinci for the 2021/2022 Academic Year. The sampling technique used is a purposive sampling technique, namely that the determination of the sample takes into account certain criteria that have been made for the subject according to the research objectives. Data analysis techniques used the normality test, homogeneity test, and hypothesis test. Based on the research that has been done, it can be concluded that the STEM-based two stay two stray learning model has an effect on critical thinking and student learning outcomes on sensory system material at SMA Negeri 2 Kerinci.
Development of STEM-Based Biology E-Module to Improve Student Learning Outcomes Irdawati Irdawati; Moralita Chatri; Kurnia Wulansari; Abdul Razak; Suci Fajrina
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.4737

Abstract

One of the factors for improving the quality of education in schools is teaching materials. Based on the results of observations at SMAN 1 Sitiung students have not used e-modules but use electronic teaching materials in the form of pdf files downloaded from the internet and only in the form of writing and a few pictures. Based on the questionnaire results, it is known that students have difficulty understanding the concept of the respiratory system material. This causes low student learning outcomes as evidenced by the daily test scores that are still below the minimum completeness criteria. So, it takes teaching materials that can help in visualizing the subject matter. This type of research is Plomp model development research which consists of three stages: the initial investigation stage, the development or prototyping stage, and the assessment stage. The research subjects were class XI students at SMAN 1 Sitiung for the 2022-2023 academic year. Validation results by the validator on e-module-based Science Technology Engineering and Mathematics (STEM) show an average grade 88.05% (very valid). Practical results by Biology teachers showed an average score of 87.50% (very practical) and 88.28% students (very practical). Effectiveness test data has effectiveness with very effective criteria in terms of cognitive competency assessment with an average of 83.59, affective with an average of 84.24, and psychomotor with an average of 81.08
Innovation in Bromelain Enzyme Production from Pineapple and Honey and Its Characteristics Weni Yulastri; Irdawati Irdawati; Yusrizal Yusrizal; Fathma Dwi Fatiha; Dwi Khayrun Nissha; Novicka Putri Anggraeni
Jurnal Penelitian Pendidikan IPA Vol 10 No 8 (2024): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i8.6976

Abstract

Bromelain enzyme is one type of protease enzyme that has the ability to hydrolyze protein peptide bonds into amino acids. Bromelain enzyme can be found in pineapple (Ananas comosus) which consists of 95% cysteine protease mixture. Bromelain is often used by athletes to treat physical injuries or minor wounds. In obtaining the bromelain enzyme found in pineapple, an isolation process is required, namely by extracting it extracellularly. The method in this study does not go through the cell breakdown process so that it is easier to separate from other impurities and does not mix with other cell materials. This study aims to develop an innovative method in the production of bromelain enzyme using pineapple and honey as raw materials and to evaluate the organoleptic properties, pH, and the presence of lactic acid bacteria. This study is a descriptive study presented in the form of graphs and images. From the research that has been done, it can be concluded that the manufacture of bromelain enzyme using pineapple and honey has succeeded in producing a product that has an organoleptic value of liking (60%), pH 3 (acid) with the number of lactic acid bacteria 2.43 cells/ml.
Pengaruh Model Pembelajaran Flipped Classroom Dengan Pendekatan Etnosains Terhadap Kemampuan Berpikir Kritis Siswa Oksa Afdarina; Moralita Chatri; Helendra; Irdawati
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.7191

Abstract

The application of the flipped classroom learning model with an ethnoscience approach trains students to learn independently in developing and sharpening critical thinking skills. The aim of the research is to determine the effect of the flipped classroom learning model with an ethnoscience approach on students' critical thinking abilities. This type of research is a quasi-experiment with a non-equivalent control group design. The sample used consisted of two classes, one class was designated as the experimental class and one class as the control class. The purposive sampling method was used to collect samples. The results of hypothesis testing using the t test show that t count is greater than table at a significance level of 0.05, which indicates that the hypothesis H0 is rejected. Thus, the flipped classroom learning model with an ethnoscience approach influences students' critical thinking abilities.
Studi Literatur: Metode Deteksi Pencemaran Mikroba pada Minuman Teh dalam Kemasan Standar ISO Melda Mei Sari; Irdawati Irdawati
TSAQOFAH Vol 6 No 4 (2026): JULI
Publisher : Lembaga Yasin AlSys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/tsaqofah.v6i4.10673

Abstract

Packaged tea beverages are processed food products widely consumed by Indonesian society and are at risk of microbial contamination due to unhygienic production, packaging, or distribution processes. This literature review aims to identify and evaluate methods for detecting microbial contaminants in packaged tea beverages based on ISO standards. The methods reviewed include the Most Probable Number (MPN/APM) based on ISO 7251:2005, Total Plate Count (TPC) based on ISO 4833-1:2013, total coliform enumeration based on ISO 4831:2006, quantitative Polymerase Chain Reaction (qPCR) based on ISO/TS 12869:2012, Enzyme-Linked Immunosorbent Assay (ELISA) based on ISO 21569:2005, membrane filtration based on ISO 8199:2018, and mold and yeast enumeration based on ISO 21527-1:2008. The results of the review show that each method has different specificity, sensitivity, and applicability according to the type of microbial contaminant and the matrix characteristics of the tea beverage sample. The MPN and TPC methods remain the main choices in routine laboratory testing because they are relatively easy to apply and compatible with national regulations, particularly the Indonesian National Standard (SNI). Meanwhile, molecular methods such as qPCR offer advantages in terms of speed and specificity but require more advanced laboratory infrastructure support. The conclusion of this review affirms that the selection of microbial detection methods needs to consider the purpose of testing, the type of target microorganism, laboratory capacity, and conformity with ISO standards and national regulations. The implications of this review can serve as a reference in determining appropriate microbiological testing methods to support quality control and food safety in packaged tea beverage products.
Pengembangan LKPD terintegrasi PJBL-STEM pada materi ekosistem untuk meningkatkan keterampilan berpikir kritis dan kolaboratif siswa sekolah menengah atas Alifda Putri Meivinia; Fitri Arsih; Heffi Alberida; Irdawati Irdawati
JRTI (Jurnal Riset Tindakan Indonesia) Vol. 11 No. 1 (2026): JRTI (Jurnal Riset Tindakan Indonesia)
Publisher : IICET (Indonesian Institute for Counseling, Education and Therapy)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29210/30036824000

Abstract

Penelitian ini bertujuan untuk mengembangkan dan menguji dampak integrasi PjBL-STEM dalam Lembar Kerja Peserta Didik (LKPD) materi Ekosistem terhadap peningkatan keterampilan berpikir kritis dan kolaboratif siswa SMA. Penelitian ini merupakan Research and Development (R&D) menggunakan model pengembangan 4-D (Define, Design, Develop, Disseminate) dari Thiagarajan, dengan tahap Disseminate tidak dilaksanakan. Subjek penelitian adalah 31 siswa kelas X SMA Pembangunan Laboratorium UNP, Padang. Instrumen pengumpulan data meliputi lembar validasi ahli, angket respons siswa, lembar observasi kolaborasi, dan tes esai berpikir kritis. Analisis dampak diukur menggunakan uji beda pre-test dan post-test serta N-Gain score. Hasil validasi ahli menunjukkan bahwa LKPD terintegrasi PjBL-STEM sangat layak digunakan dengan persentase kevalidan 91% dan kepraktisan 95%. Implementasi LKPD menunjukkan adanya perbedaan yang signifikan pada hasil pre-test dan post-test. Uji N-Gain menunjukkan dampak peningkatan keterampilan berpikir kritis sebesar 0,67 (kategori sedang) dan keterampilan kolaboratif sebesar 0,89 (kategori tinggi). Disimpulkan bahwa integrasi PjBL-STEM dalam LKPD Ekosistem tidak hanya valid dan praktis, tetapi juga berdampak positif dan signifikan dalam memberdayakan keterampilan berpikir kritis dan kemampuan kolaborasi siswa secara holistik.
PENGARUH VARIASI VOLUME CAIRAN RUMEN SAPI TERHADAP PRODUKSI BIOGAS BERBASIS KOTORAN SAPI Echa Azkia Afandi; Alya Hamdah; Irdawati; Dwi Hilda Putri
JURNAL BIOSENSE Vol 9 No 2 (2026): Edisi April 2026
Publisher : Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i2.7977

Abstract

Kebutuhan energi yang terus meningkat mendorong pengembangan sumber energi alternatif yang lebih ramah lingkungan, salah satunya biogas yang dihasilkan melalui proses fermentasi anaerob bahan organik. Limbah peternakan seperti kotoran sapi memiliki potensi besar sebagai substrat biogas karena kandungan bahan organiknya yang tinggi. Selain itu, cairan rumen sapi yang kaya akan mikroorganisme dan enzim pendegradasi bahan organik diduga dapat berperan sebagai inokulum untuk meningkatkan efisiensi proses fermentasi. Penelitian ini bertujuan untuk mengkaji pengaruh variasi volume cairan rumen sapi terhadap produksi biogas berbasis kotoran sapi. Metode yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan tiga perlakuan, yaitu tanpa penambahan cairan rumen (P1), penambahan 160 mL cairan rumen (P2), dan penambahan 320 mL cairan rumen (P3), masing-masing dengan dua ulangan. Fermentasi dilakukan selama 20 hari dalam kondisi anaerob dalam reaktor dengan balon karet sebagai penampung gas. Volume biogas diamati setiap 5 hari berdasarkan perubahan volume balon. Hasil pengamatan menunjukkan adanya peningkatan produksi biogas seiring bertambahnya volume cairan rumen yang digunakan. Perlakuan P3 memberikan hasil tertinggi dibandingkan perlakuan lainnya, diikuti oleh P2 dan P1. Hal ini mengindikasikan bahwa cairan rumen berpotensi meningkatkan aktivitas mikroorganisme dalam proses degradasi bahan organik menjadi gas metana sehingga berkontribusi terhadap peningkatan produksi biogas.
POLA PRODUKSI BIOENERGI SEL AMOBIL Bacillus licheniformis KGEB16 SISTEM MFC PADA REUSABILITAS KETIGA Shella Anggraeni; Irdawati; Linda Advinda; Siska Alicia Farma
JURNAL BIOSENSE Vol 9 No 2 (2026): Edisi April 2026
Publisher : Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i2.7979

Abstract

Penggunaan bahan bakar fosil yang terus menurun mendorong pengembangan energi terbarukan, salah satunya melalui teknologi Microbial Fuel Cell (MFC). Penelitian ini bertujuan menganalisis pola produksi bioenergi sel amobil Bacillus licheniformis strain KGEB16 dalam sistem MFC pada siklus reusabilitas ketiga. Sel bakteri diamobilisasi menggunakan matriks natrium alginat 5% (b/v) dengan metode penjebakan (entrapment), dan sistem MFC bertipe dual chamber digunakan sebagai bioreaktor. Tegangan listrik diukur setiap 2 jam selama 24 jam dengan 4 ulangan (n = 4) menggunakan multimeter digital. Hasil penelitian menunjukkan bahwa tegangan tertinggi tercatat pada jam ke-0 sebesar 0,553 V dan menurun secara konsisten hingga 0,117 V pada jam ke-24, dengan total akumulasi tegangan sebesar 3,277 V. Pola penurunan berlangsung dalam dua fase: penurunan tajam pada jam ke-0 hingga jam ke-8 akibat deplesi substrat, dilanjutkan penurunan gradual hingga jam ke-24 karena keterbatasan difusi substrat ke dalam matriks beads. Meskipun demikian, aktivitas elektrogenesis B. licheniformis KGEB16 masih terdeteksi hingga akhir pengamatan, yang menunjukkan bahwa sel amobil dalam matriks alginat masih mampu mempertahankan aktivitas metaboliknya pada reusabilitas ketiga.
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abror Abror Agustien Agustien Ahmad, Nurafni Fajarna Do Alifda Putri Meivinia Alya Hamdah Andrian, Richard Anthoni Agustien Apriansa, Apriansa Ardi Ardi Armiliandi, Rada Aryeni Fatwah, Monika Az-Zahra, Fauziah Azwir Anhar Bob Refianto Deni Kurniati Des M Des Desi Nurmayenti Dezi Handayani Dezi Handayani Dwi Hilda Putri Dwi Hilda Putri Dwi Khayrun Nissha Echa Azkia Afandi Elfah rahma Elsa Sri Handayani Eria Marina Sepriyani Fahra Fahra Fahra Fahra Fajri, Dilham Hidayatul Faradilla Rattriana Fathma Dwi Fatiha Fauzana Ahmad Feby Yeriska Fevria, Resti Fitri Angraini Fitri Arsih Fitri Arsih Fitri Arsih Francantika, Manja Ghiffari, Muhammad Gustina Indriati Heffi Alberida Helendra Helpida Helpida Ilsa Septia Putri Inayatul Fatia Indrawani Matondang Irma Leilani Jannah Koftiah Khalif Alfajri Kurnia Wulansari Larashinda, Mentari Lenty Supriwardi Linda Advinda Linda Advinda Lufri Lufri Luthfi, Afdal Mades Fifendy Marissa Angelina Maulina, Adinda Rizky Mei Niarti Melda Mei Sari Melsi Pratiwi Yusni Monhartini Monhartini Moralita Chatri Muhamad Sholichin Muhammad Aulia Rahman Muhammad Deedat Ayasy Muhyiatul Fadilah Mulia Mulia Mulia Mulyadi, Rival Muskhir, Mukhlidi Nada Nafion Nia Ramadhanti Nisa Afifah Novicka Putri Anggraeni Nur Shofiatun Nisa Nur Vaizi Oksa Afdarina Putri Andriani Putri Ardila Sari Putri Rachma Auliya Putri Rahma Padilla Putri Sarinami Rahmi, Elva Refni Syahleli Afni Rizka Putri Alti Roza Yolanda Sasmeri Sasmeri Selaras, Ganda Hijrah Shella Anggraeni Siska Alicia Farma Sri Wahyuni Suci Fajrina Suci Fajrina Suci Fajrina Suherman, Donny Summaiati, Titi Syahrastani, Syahrastani Syamsuardi Syamsuardi Syamsurizal S Threesia Anugrah Ilahi Tomi Apra Santosa Uthami, Feranis Nadia Vauzia Vauzia Vestimarta, Aldi Wahyuda Violeta Violita Violita Violita Violita Viona, Alda Wahyuda Vestimarta, Aldi Wardah Hayati, Wardah Wella Antika Weni Yulastri Windi Nopitasari Wirda Taufik Witri Nofita Safitri Y Rilda Yetria Rilda Yuandry, Syafrina Yuni Ahda Yusrizal Y Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Zahara Nurfatihah Z Zakri, Dwika Febriana Zulyusri, Zulyusri