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All Journal PROSIDING SEMINAR NASIONAL BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Pro Food Jurnal Farmasi Sains dan Komunitas Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pengabdian Pada Masyarakat Indonesian Journal of Chemistry Jurnal Kesehatan Prima Gorontalo Journal of Public Health INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Jurnal Pengabdian Masyarakat Indonesia Majalah Ilmiah UPI YPTK Research Fair Unisri JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Buletin Pengabdian Prosiding Seminar Nasional Unimus
Stalis Norma Ethica
Universitas Muhammadiyah Semarang
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Journal : Research Fair Unisri

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DETECTION OF VIRULENCE GENES phoP AND phoQ IN Salmonella spp. USING IN SILICO POLYMERASE CHAIN REACTION Stalis Norma Ethica; Hayatun Fuad; Nur Hidayah; Sri Sinto Dewi; Aditya Rahman Ernanto; Ayu Rahmawati Sulistyaningtyas; Richard David Silvestre; Sri Darmawati
RESEARCH FAIR UNISRI Vol. 4 No. 1 (2020): RESEARCH FAIR UNISRI
Publisher : Universitas Slamet Riyadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (701.748 KB) | DOI: 10.33061/rsfu.v4i1.3414

Abstract

Detection of Salmonella bacteria based on their virulence genes is among essential steps in the eradication of clinical infection by bacteria. In this study, two pair of primers, PhoPF-PhoPR: 5’- CCGCGCAGGAAAAACTCAAA-3’ and 5’-ATCTGTTCCAGCATCACCGG -3’ as well as PhoQF-PhoQR: 5’-AGAGATGATGCGCGTACTGG-3’ and 5’- CAGACGCCCCATGAGAACAT-3’, had been successfully designed using Primer3Plus to detect the presence of phoP and phoQ genes in Salmonella spp. Using genomic DNA of 44 genomic data of Salmonella spp. as templates, PhoPF-PhoPR could produce 520-bp amplicon, while PhoQF-PhoQR could result in 598-bp amplicon. Results of in silico PCR showed that both pairs of primers PhoPF-PhoPR and PhoQF-PhoQR could detect only Salmonella enterica species, and no Salmonella bongori species could be detected based on phoP and phoQ sequences. Both pairs of PhoPF-PhoPR and PhoQF-PhoQR primers were also able to detect the virulence genes in most of the studied subspecies of Salmonella enterica available in silico database unless Arizona subspecies. As conclusion, based on this in silico study, phoP and phoQ genes appeared to be biomarkers for Salmonella enterica species. Both pairs of primers designed in this study has potential to be used as detection tool to differentiate species Salmonella enterica from Salmonella bongori, and also to distinguish S.enterica subsp. enterica from subsp. Arizonae.Keywords: Gene detection, bacterial virulence, phoP, phoQ, Salmonella spp.
Co-Authors A.A. Ketut Agung Cahyawan W Abdillah, Rifqi Aditya Rahman Ernanto Aditya Rahman Ernanto Aditya Rahman Ernanto Afriansyah, M. Ardi Afriansyah, Muhammad Ardi Agus Sabdono Agus Suyanto Agus Suyanto Aida Zakiyatul Fikriyah Ainutajriani Ainutajriani Ainutajriani, Ainutajriani Alisha Triwahyuni Alvira Intar Bella Cahya Ana Hidayanti Mukaromah Ana Hidayati Mukaromah Ana Hidayati Mukaromah Andri Sukeksi Aprianti, Nurannisa Fitria Aprilia Indra Kartika, Aprilia Indra Arfiyanti, Mega Pandu Arham, Chaerul Aufit Fahima Aulia Harun Aulia Meirizka Saputra Ayanti, Bio Putri Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Cendani, Tinezia Rivan Dewi Seswita Zilda Dhea Ayu Lestari Dian Purwo Jatinging Putri Diatri, Devita Dilin Rahayu Nataningtyas Diyanah, Dina Dwi Pamaya Elly Rustanti Ellyka Purwaningrum Endang Semiarti Endang Semiarti Endang Tri Wahyuni Maharani Ernanto, Aditya Rahman Fanda Indriyani Fandhi Adi Wardoyo Farabi, Meka Faizal Fauziyyah, Dinah Febrianti, Pepi Feri Feri Fitrah, Reza Fuad, Hayatun Hayatun Fuad Hikmah, Annisa Nurul Ika Dyah Kurniati Istini Istini Izza, Naimatul JAKA WIDADA Jaka Widada Janah, Inayah Qori Nur Jimat, Resi Tondho Jumardi, Muhamad Kazi Mohammad Zillur Rahman Khoirunisa, Asiva L. Hartanto Nugroho Lestari, Citra Rahayu Maya Dian Rakhmawatie Maya Linda Shafira Meturan, Dessy Marselina Meyliana, Adinda Mirza, Sa’if Revallindo Mudyawati Kamaruddin Muhammad Ardi Afriansyah Muhammad Ardi Afriansyah Mukarromah, Ana Hidayanti Musa, Susan Hasanah Mutia Srikandi Fitria Nanik Marfu'ati Ningsi, Ana Lestari Noverson Lidaya Nur Hidayah Nur Hidayah Nur Hidayati Nur Hidayati Nurcahyono Nurcahyono Nurcahyono Nurcahyono, Nurcahyono Nurrahman Nurrahman Nurrahman Nurrahman Nurrahman Nurrahman Oedjijono Oedjijono, Oedjijono Okta Yosiana Dewi Pratama, Aji Sukma Puji Lestari Putri, Vivi Vahtalava Radna Safitri Rafiyanto, Achmad Ragil Saptaningtyas Ragil Saptaningtyas Ragil Saptaningtyas, Ragil Richard David Silvestre Riswan Riswan Rivana Ariyadi Rosyida Azis Rizki Sakti Imam Muchlissin Samiroh Samiroh, Samiroh Setia Iriyanto Setia Iriyanto Silvestre, Richard David Siska Nurprihandayani Siti Aminah Siti Aminah Siti mutmainnah Sofyan Yosias Beslar Sri Darmawati Sri Darmawati Sri Darmawati Sri Elvira Sri Sinto Dewi Sri Sinto Dewi Sri Sinto Dewi Sri Sinto Dewi suardi suardi Sukowiyono Sukowiyono Sukowiyono Sukowiyono Sulistyaningtyas, Ayu Rahmawati Sultistyaningtyas, Ayu Rahmawati Tamimi, Iqbal Theresia Liyana Sejati Tri Joko Raharjo Tri Joko Raharjo Viandy, Amartya Desta Vicky Mahendra Nurzhulian Vradea Pramesta Wa Ode Inayatul Wahyuni, Arti Widyani, Dhiyananda Wikanastri Hersoelistyorini Wikanastri Hersoelistyorini Yuni Nurkuntari Zakiya, Roma Dhona Beauty