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Journal : PROSIDING SEMINAR NASIONAL

SAMPLING MIKROBIOLOGI LIMBAH BIOMEDIS RUMAH SAKIT DI KOTA SEMARANG JAWA TENGAH Stalis Norma Ethica; Sakti Imam Muchlissin; Ragil Saptaningtyas; Agus Sabdono
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Abstract

The present study aimed to propose a technical strategy of sampling for obtaining microbiological samples from liquid biomedical wastes generated by hospitals in Semarang City, Central Java Province, Indonesia.  The samples will be used to evaluate bioremediation ability of the hydrolytic bacteria isolated from the waste. It was hoped that the proposed strategy could help to guide researchers about appropriate sampling practices on hospital biomedical wastes from IPAL of Indonesian hospitals. As mandated by the Indonesian law, the handling of hospital liquid waste in Indonesia should follow Permenkes No.1204/2004, while the liquid waste standard should meet the Kepmen LH No.58/1995, reaffirmed by Perda Provinsi Jawa Tengah No.10/2004 about liquid waste standard issued by the Governor of Central Java. Based on formal regulations and safety aspects, it is proposed that the strategy for a proper microbiological sampling practice in terms of hospital liquid biomedical waste should include: (1) Permit letter from hospital where sampling location is; (2) Relevant sampling method based on research purpose (3) Standard, anti-leaked, properly labelled sampling containers(4) Proper sampling equipment based on sampling purpose (5) Adequate sampling transport equipment; (6) The use of standard personal protection equipment (PPE); and (7) Vaccine for the sampling workers (hepatitis B vaccination ismandatory).  Keywords: microbiological sampling, hospital biomedical waste, microbial bioremediation, sampling strategy,Indonesian sampling regulation
PROFIL PROTEIN BERBASIS SDS-PAGE PADA ULAT SAGU HASIL PENGERINGAN DENGAN GARAM DAN TANPA GARAM Aufit Fahima; Ana Hidayati Mukaromah; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Sago larvae is a potential source of animal protein because it is easy to digest, but it has a weakness, which is easy to rot. To avoid decay can be preserved by drying and salting the sago larvae. The purpose of this study was to determine the protein profile of the sago larvae which was dried with salt and without salt based on SDS-PAGE. The research sample used sago larvae with treatment:, 1) heated in oven with salt and 2) without salt, and also 3) salted without drying at 50 °C using an oven for 1 hour. The results of the study were obtained in the control of 26 bands and there were 6 major bands and 20 minor bands. In samples dried at 50°C using an oven for 1 hour without salting has 21 bands and  there  are  4  major  bands  and  17  minor  bands.  In  the  salted  sample concentration of 10% (b/b) for 1 hour it had 24 bands and there were 5 major bands and 19 minor bands. While the samples were dried and salted with a concentration of 10% (b/b) at a temperature of 50°C using an oven for 1 hour had 19 ribbons and there were 3 major bands and 16 minor bands. Based on the results of this study, the preservation of sago larvae by salting of 10% (b/b) is more recomended better than heating by oven for 1 hour at 50°C. In the salting  preservation,  number  of  protein  bands  of  sago  larvae  based  on  its protein profile decreases less than that in heating preservation . Keywords : Sago larvae, Drying, Salting, Protein Profile, SDS-PAGE.
Co-Authors A.A. Ketut Agung Cahyawan W Abdillah, Rifqi Aditya Rahman Ernanto Aditya Rahman Ernanto Aditya Rahman Ernanto Afriansyah, M. Ardi Afriansyah, Muhammad Ardi Agus Sabdono Agus Suyanto Agus Suyanto Aida Zakiyatul Fikriyah Ainutajriani Ainutajriani Ainutajriani, Ainutajriani Alisha Triwahyuni Alvira Intar Bella Cahya Ana Hidayanti Mukaromah Ana Hidayati Mukaromah Ana Hidayati Mukaromah Andri Sukeksi Aprianti, Nurannisa Fitria Aprilia Indra Kartika, Aprilia Indra Arfiyanti, Mega Pandu Arham, Chaerul Aufit Fahima Aulia Harun Aulia Meirizka Saputra Ayanti, Bio Putri Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Cendani, Tinezia Rivan Dewi Seswita Zilda Dhea Ayu Lestari Dian Purwo Jatinging Putri Diatri, Devita Dilin Rahayu Nataningtyas Diyanah, Dina Dwi Pamaya Elly Rustanti Ellyka Purwaningrum Endang Semiarti Endang Semiarti Endang Tri Wahyuni Maharani Ernanto, Aditya Rahman Fanda Indriyani Fandhi Adi Wardoyo Farabi, Meka Faizal Fauziyyah, Dinah Febrianti, Pepi Feri Feri Fitrah, Reza Fuad, Hayatun Hayatun Fuad Hikmah, Annisa Nurul Ika Dyah Kurniati Istini Istini Izza, Naimatul JAKA WIDADA Jaka Widada Janah, Inayah Qori Nur Jimat, Resi Tondho Jumardi, Muhamad Kazi Mohammad Zillur Rahman Khoirunisa, Asiva L. Hartanto Nugroho Lestari, Citra Rahayu Maya Dian Rakhmawatie Maya Linda Shafira Meturan, Dessy Marselina Meyliana, Adinda Mirza, Sa’if Revallindo Mudyawati Kamaruddin Muhammad Ardi Afriansyah Muhammad Ardi Afriansyah Mukarromah, Ana Hidayanti Musa, Susan Hasanah Mutia Srikandi Fitria Nanik Marfu'ati Ningsi, Ana Lestari Noverson Lidaya Nur Hidayah Nur Hidayah Nur Hidayati Nur Hidayati Nurcahyono Nurcahyono Nurcahyono Nurcahyono, Nurcahyono Nurrahman Nurrahman Nurrahman Nurrahman Nurrahman Nurrahman Oedjijono Oedjijono, Oedjijono Okta Yosiana Dewi Pratama, Aji Sukma Puji Lestari Putri, Vivi Vahtalava Radna Safitri Rafiyanto, Achmad Ragil Saptaningtyas Ragil Saptaningtyas Ragil Saptaningtyas, Ragil Richard David Silvestre Riswan Riswan Rivana Ariyadi Rosyida Azis Rizki Sakti Imam Muchlissin Samiroh Samiroh, Samiroh Setia Iriyanto Setia Iriyanto Silvestre, Richard David Siska Nurprihandayani Siti Aminah Siti Aminah Siti mutmainnah Sofyan Yosias Beslar Sri Darmawati Sri Darmawati Sri Darmawati Sri Elvira Sri Sinto Dewi Sri Sinto Dewi Sri Sinto Dewi Sri Sinto Dewi suardi suardi Sukowiyono Sukowiyono Sukowiyono Sukowiyono Sulistyaningtyas, Ayu Rahmawati Sultistyaningtyas, Ayu Rahmawati Tamimi, Iqbal Theresia Liyana Sejati Tri Joko Raharjo Tri Joko Raharjo Viandy, Amartya Desta Vicky Mahendra Nurzhulian Vradea Pramesta Wa Ode Inayatul Wahyuni, Arti Widyani, Dhiyananda Wikanastri Hersoelistyorini Wikanastri Hersoelistyorini Yuni Nurkuntari Zakiya, Roma Dhona Beauty