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Journal : Jurnal Medik Veteriner

Pengaruh Paparan Uap Rokok Elektrik Terhadap Gambaran Histopatologi Organ Pulmo Tikus Putih (Rattus norvegicus) Ardy, Carolina; Hamid, Iwan Sahrial; Yudhana, Aditya; Widjiati, Widjiati
Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (913.565 KB) | DOI: 10.20473/jmv.vol3.iss1.2020.38-44

Abstract

Studi ini bertujuan untuk mengidentifikasi pengaruh paparan uap rokok elektrik terhadap gambaran histopatologi organ pulmo. Tikus putih yang digunakan sebanyak 20 ekor yang terbagi menjadi 5 kelompok, kontrol (K) adalah yang tidak diberikan paparan uap rokok elektrik dan kelompok perlakuan yang diberikan paparan uap dengan dosis yang berbeda, kelompok Perlakuan I (0,3 mg/ml), P2 (3 mg/ml), P3 (12 mg/ml), P4 (36 mg/ml). Setelah 14 hari perlakuan, tikus putih dieuthanasia dan dinekropsi serta organ pulmo diawetkan untuk dilakukan pembuatan preparat histopatologi dengan menggunakan pewarnaan Hematoxilin-Eosin. Hasil menunjukkan terdapat kerusakan pada gambaran histopatologi pulmo yang diamati pada perbesaran 400x. analisis data menggunakan Kruskal-Wallis dan dilanjutkan dengan uji Mann Whitney menunjukkan perbedaan yang signifikan (p<0.05). Rata-rata kerusakan organ pulmo tikus putih adalah kontrol (K)=3.00; (P1)=6.00; (P2)=12.00; (P3)=13.00 dan (P4)= 18.50 dan dosis minimal dari nikotin adalah 3 mg/ml. Kesimpulan, paparan uap rokok elektrik dapat menyebabkan kerusakan yang signifikan pada organ pulmo tikus putih.
Seroprevalensi Antibodi Newcastle Disease (ND) pada Itik di Desa Temuasri, Sempu, Banyuwangi Rozi, Fachrur; Rahmahani, Jola; Hamid, Iwan Sahrial; Yudhana, Aditya; Praja, Ratih Novita
Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (592.26 KB) | DOI: 10.20473/jmv.vol3.iss1.2020.108-113

Abstract

Tujuan penelitian ini untuk mendeteksi titer antibodi ND pada itik di Desa Temuasri Kecamatan Sempu Kabupaten Banyuwangi. Deteksi antibodi ND diperiksa dengan uji Hemaglutinasi (HA) dan Hambatan Hemaglutinasi (HI). Penelitian menggunakan desain penelitian non-eksperimental dengan metode survei deskriptif. Kegiatan penelitian ini mengambil 72 sampel darah itik di Desa Temuasri Kecamatan Sempu Kabupaten Banyuwangi dengan metode cross-sectional. Serum dipisahkan dari bekuan darah, kemudian serum dipindahkan ke dalam microtube. Sebelum melakukan uji HA dan uji HI, sampel harus diberi perlakuan dengan menggunakan Red Blood Cell (RBC) 100% dan diinaktifasi ke dalam waterbath pada 56oC selama 30 menit, tujuannya untuk menghilangkan aglutinin non-spesifik dan menonaktifkan reaksi non-spesifik serum. Uji HI positif ketika menunjukkan pengendapan eritrosit berbentuk titik pada dasar sumuran micoplate. Hasil penelitian ini menunjukkan 20 (27.77%) dari 72 sampel positif ND.
Cemaran Jamur pada Pasir Sarang Semi Alami Penyu Hijau (Chelonia mydas) di Taman Nasional Meru Betiri Banyuwangi Muhammad, Abdul Latif; Rahmahani, Jola; Hamid, Iwan Sahrial; Praja, Ratih Novita
Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (599.688 KB) | DOI: 10.20473/jmv.vol3.iss1.2020.1-8

Abstract

Tujuan dari penelitian ini adalah untuk mendeteksi adanya jamur pada sarang semi alami penyu lekang di Taman Nasional Meru Betiri Banyuwangi. Sebanyak 1 gram pasir pada 22 sarang penyu hijau digunakan sebagai sampel. Isolasi dan identifikasi dilakukan dengan pengamatan karakteristik makroskopis dari pertumbuhan koloni dan karakteristik mikroskopis dengan pewarnaan lactophenol cotton blue. Berdasarkan hasil penelitian sebanyak 3 sampel (13.6%) negatif dan 19 sampel (83.4%) positif terhadap pertumbuhan jamur. Beberapa jenis jamur yang telah ditemukan antara lain Aspergillus sp., Cunninghamella sp., Fusarium sp. dan Trichoderma sp.
Improvement of Feed Processing Skills using Probiotics in Glondok and Panggang Village Communities, Banyuwangi Hamid, Iwan Sahrial
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.170-174

Abstract

This community empowerment aimed to introduce probiotics and improve breeders' skills in processing feed. Community empowerment activities that have been carried out include providing probiotics for ruminants which are given directly to breeders by visiting the location of the beef cattle pen. The community empowerment program was carried out for 5 months including counseling, training, evaluation, and training for cadres. From the evaluation results, there was an increase in affective from 20% to 60%, cognitive from 28% to 75%, and psychomotor from 8% to 80%. The cadres who were assigned will be assisted so that the development of livestock after being given probiotics can be monitored continuously.
Sparganosis pada Ular Jali (Ptyas korros) Fransiska, Eunike Melanda; Hamid, Iwan Sahrial; Yudhana, Aditya; Triakoso, Nusdianto; Praja, Ratih Novita
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.258-262

Abstract

Ular Ptyas korros atau ular jali merupakan salah jenis ular yang ada di Indonesia.. Berbagai penyebab kepunahan adalah salah satunya masalah kesehatan seperti penyakit yang disebabkan parasit. Laporan kasus ini bertujuan untuk mengidentifikasi cacing yang ditemukan pada ular P. korros yang telah mati, metode identifikasi menggunaka pewarnaan carmine dan diamati dibawah mikroskop dengan perbesaran 40x dan 100x. Hasil identifikasi ditemukan cacing pita Spirometra sp. sebanyak 18 ekor di  daerah subkutan dan muskulus sepanjang tubuh ular. Cacing Spirometra sp. menyebabkan penyakit spargonosis yang bersifat zoonosis atau dapat menular pada manusia.
Serodeteksi Brucellosis dengan Metode Rose Bengal Test dan Complement Fixation Test pada Sapi Perah di Banyuwangi Wilujeng, Estiary; Suwarno, Suwarno; Praja, Ratih Novita; Hamid, Iwan Sahrial; Yunita, Maya Nurwartanti; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.188-195

Abstract

Tujuan penelitian adalah untuk mendeteksi adanya antibodi dari Brucella pada ternak sapi perah betina di Banyuwangi menggunakan uji Rose Bengal Test (RBT) untuk uji cepat dan dikonfirmasi dengan menggunakan Complement Fixation Test (CFT). Sebanyak 84 sampel dari 516 sapi perah betina di Banyuwangi, kemudian diambil secara acak. Sampel yang diambil berasal dari serum sapi perah betina. Hasil penelitian ditemukan dua sampel yang dinyatakan positif Brucellosis dengan uji RBT dan CFT. RBT merupakan uji screening yang dapat memberikan hasil positif palsu, dan kemudian dikonfirmasi dengan uji CFT untuk melihat titer hasil vaksin atau infeksi alam. Hasil positif pada uji RBT memiliki hubungan dengan hasil titer CFT karena titer antibodi pada RBT dapat diukur dari derajat aglutinasi yang terbentuk. Hasil seropositif penyakit Brucellosis di Banyuwangi sebesar 2%.
Effectiveness of Clove Flower Extract (Syzygium Aromaticum L.) as Analgesic on Licking Time Reaction in Male Mice with Formalin Induction Puspitasari, Vina; Wahjuni, Retno Sri; Saputro, Amung Logam; Hamid, Iwan Sahrial; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.226-230

Abstract

This research is about testing the effectiveness of clove flower extract (Syzygium aromaticum L.) as an analgesic against the beating time caused by formalin male mice. This research is experimental with the Complete Random Design formula producing four times repetition (N = 4) and divided into five treatment groups (t = 5). Data were analyzed using ANAVA factorial test then followed by Duncan test. All tests are carried out with Product Solutions and Service Statistics (SPSS). The results of research and statistical analysis prove that clove flower extract at a dose of 1% is an effective dose compared to other treatment groups, seen from the amount of time licking male mice  which is smaller than other treatment groups. It can be concluded that the administration of clove flower extract has analgesic effectiveness against the reaction of male mice induced by formalin. The concentration of clove flower extract 1% is the most effective in reducing licking time.
Gamal (Gliricidia Sepium) Leaf Extract Increase Mortality of Fasciola gigantica In Vitro Zulianti, Brillia; Hamid, Iwan Sahrial; Yudhana, Aditya; Rahmahani, Jola; Yunita, Maya Nurwartanti; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.193-198

Abstract

Fasciolosis is a disease caused by infection with liver fluke (Fasciola sp). Fasciolosis is a parasitic disease that is important because it can cause high economic losses. The aim of this study was to investigate the effect of gamal (Gliricidia sepium) leaf extract against mortality of Fasciola gigantica in vitro study. In present study was completely randomized design with six treatments and each treatment were divided into four replications. A total of 240 specimens from infected cattles were used in this study. The observation and recording of F. gigantica mortality in all treatments with concentrations of gamal leaf extract P1 (1%), P2 (2%), P3 (3), P4 (4%), K + (Nitroxynil 2%) and K- (physiological NaCl) rate was done in 30, 60, 90, 120, 150, 180, 210 and 240 minutes, respectively. The mortality of F. gigantica was confirmed if there was no movement when triggered by anatomy tweezers then dipped in slightly warm water (50 15í¢„Æ’"> ). The data was analyzed using ANAVA Faktorial and continued with Duncan Multiple Range Test. The results of this study were shown the lowest and the highest mortality rate recorded at minutes 30 and 240 respectively. Moreover, the mean of mortality rate were peaked in nitroxynil (K+) and 1% gamal leaf extract treatment, while the lowest mean of mortality rate occurs in NaCl (K-) treatment. Present study conclude that gamal leaf extract has anthelmintic effect on the mortality rate of Fasciola gigantica. Gamal leaf extract with 1% concentration was claimed to be the most effective.
Most Probable Number of Escherichia Coli in Fresh Milk at KPSP Ijen Makmur, Licin Sub-District, Banyuwangi Wijaya, Achmad Arby; Hamid, Iwan Sahrial; Yunita, Maya Nurwartanti; Tyasningsih, Wiwiek; Praja, Ratih Novita
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.207-212

Abstract

The study aimed to identify the total number of E. coli used in fresh cow milk in KPSP Ijen Makmur. The sample was used by as many as 16 samples from a group of cattle farmers. E. coli analyzed using Most Probable Number (MPN), 3 series of tubes. Before continuing the MPN test the milk must be diluted, 25 ml of milk was poured into the dilution of the 225ml peptone water buffered then homogenized for 2 minutes. MPN consist of presumptive coliform test if positive was found of gas and was cloudy, presumptive faecal coliform test positive was found in the gas and was cloudy, confirmed E.coli test if there was a black colony with or metallic green, continued by a biochemical test with red ring-positive Indole, Methyl Red positive the color is red, negative Voges Paskauer if there is no change in color, and negative citrate will turn green. Results showed that from the 16 samples of fresh milk used there were 7 samples of fresh milk that exceeded the contamination limit E. coli or < 3 apm /ml. Conclusion, number of E. coli in fresh milk at KPSP Ijen Makmur 43.75% of the total sample exceeded maximum contamination.
Effect of Robusta Coffee Extract on Histopathological in Mice Testes Induced with Monosodium Glutamat Plumeriastuti, Hani; Weliyani, Trima; Hamid, Iwan Sahrial; Hidanah, Sri; Prastiya, Ragil Angga; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.243-248

Abstract

Monosodium glutamate (MSG) is a sodium salt from glutamic acid which is currently very popular to be used as a food flavoring ingredient to stimulate appetite. Exessive consumption of MSG can disrupt the balance of antioxidants and ROS, and cause the negative effects of oxidative stress on testes. Coffee has high chlorogenic acid, function for antioxidants and reduce the negative effect on cell damage in the testes. This study aims to analyze the effect of robusta coffee extract on histological of mice testes induced with MSG. This study was completely randomized design (CRD), using 20 male mice for 5 treatment groups: K+ (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) orally for 42 days. Histophatological scores were analyzed with Saphiro-Wilk Test and Anova. There is no effect of increasing testicular weight. 0,12 mg MSG can cause a decrease in the number of spermatogonia and spermatocytes. 0,2 mg robusta coffee extract can maintain the number of spermatogonia and spermatocytes.
Co-Authors A.A. Ketut Agung Cahyawan W Abdul Samik, Abdul Annise Proboningrat Ardy, Carolina Arianto, Intan Diah Safitri Arimbi Budi Utomo Chairul Anwar Dermayati, Cyntia Zain Dermayati, Cyntia Zain Desy Fatmawati Dewi, Sapna Kurnia Dwi, Welyanto Kurniawan E Bimo Aksono Erma Safitri Estoepangestie, Agnes Theresia Soelih Fachrur Rozi Faisal Fikri Fedik Abdul Rantam Ferdiansyah, Muhammad Billy Firdausyi, Aulia Amatul Fransiska, Eunike Melanda Hani Plumeriastuti Harelas, Genoveva Callista Hermadi, Herry Agus Herupradoto, Eduardus Bimo Aksono Hestiana, Eka Pramyrtha Hidanah, Sri IMAM MUSTOFA Ira Sari Yudaniayanti Ivanalee, Angela Swasti Jeanita, Winny Kadek Rachmawati Kariyaningtias, Vivi Kartikasari, Anjani Marisa Kresnamurti, Angelica Kusumaningrum, Putri Anggraheni Lake, Werenfridus Kono Lastoaji, Firman Lianny Nangoi Lokapirnasari, Widya Paramitha Lovela, Aprinda Ratna Lucia Hendriati Luqman, Epy Muhammad Mafruchati, Maslichah Mardatillah, Meidy Meles, Dewa Ketut Muhammad, Abdul Latif Mustofa Helmi Effendi Muzhaffar, Adam Maulana Novitasari, Seline Putri Nugrahani, Anggi Dwi Nusdianto Triakoso octavia, Arinda Praja, Ratih Novita Pramesti, Nugraheni Anggara Prastiya, Ragil Angga Primarizky, Hardany Puspitasari, Vina Putrianirma, Rizki Rahmahani, Jola Rahmatullah, Aldin Akbar Rahmi Sugihartuti, Rahmi Rasdianto, Dwi Damayanti Ratna Damayanti Restiadi, Tjuk Imam Retno Bijanti Salahuddin, Muhammad Soleh Santoso, Kuncoro Puguh Saputro, Amung Logam Sarudji, Suryanie Satria, Rafif Galih Solikhah, Tridiganita Intan Sri Mulyati Sri Pantja Madyawati, Sri Pantja Sucita, Ricky Eka Suherni Susilowati Sukmanadi, Mohammad Sunarso, Agus Supriyadi Supriyadi Suresti, Suresti Surya, Regiskha Hermandanie Suwarno Suwarno Tatik Hernawati Teguh Widodo Tito, Muhammad Tri Wahyu Suprayogi Tyasningsih, Wiwiek Wahjuni, Retno Sri Wahyudi, Alif Ekacahya Weliyani, Trima Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Wijaya, Achmad Arby Willyanto, Jennifer Rachel Wilujeng, Estiary Wurlina, W Yudhana, Aditya Yunita, Maya Nurwartanti Yuseni Kusuma P Yustinasari, Lita Rakhma Zahro, Vida Seanita Zulianti, Brillia