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All Journal Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmiah Mahasiswa Veteriner Bulletin of Data Science Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Buletin Pengabdian Heca Journal of Applied Sciences Jurnal Medika Veterinaria Peternakan Abdi Masyarakat (PETAMAS)
Muhammad Hanafiah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Journal : Jurnal Veteriner

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PEMERIKSAAN LEUCOCYTOZOONOSIS PADA BROILER DAN ITIK MENGGUNAKAN METODE GERUSAN ORGAN DAN HAPUSAN DARAH (EXAMINATION OF LEUCOCYTOZOONOSIS IN BROILERS AND DUCKS USING ORGAN MASHED METHODS AND BLOOD SMEAR *) Muhammad Hanafiah; Rusli Sulaiman; Nuraini Latif
Jurnal Veteriner Vol 8 No 2 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The study has been done to examine Leucocytozoonosis case using organ mashed method in broiler and ducks slaughtered in chickens slaughter house in Banda Aceh Nanggroe Aceh Darussalam. From each of broiler and ducks fifty organs were collected for sample. Before those animals were killed blood smear were taken to find merozoites and gametosites stadium while smashed organs were made to find schizont stadium. The number of schizont of Leucocytozoonosis were analyzed descriptively. Result of research indicate that was higher positiveLeucocytozoonosis with mashed method better than blood smear in broiler and duck. The prevalence of Leucocylozoonosis with blood smear respectively are 30 % and 24 % from broiler while by using organ smashed methods are 58 % and 54 % respectively.
Produksi dan Isolasi Protein Membran Stadium Bradizoit Toxoplasma gondii : Suatu Usaha untuk Mendapatkan Material Diagnostik dalam Mendiagnosa Toksoplasmo Muhammad Hanafiah; Wisnu Nurcahyo; Mufti Kamaruddin; Fadrial Karmil
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted to isolate the membrane protein of Toxoplasma gondii at bradyzoid stage forthe development of intradermal diagnostic test in livestock infected by the parasite. Toxoplasma wasinitially collected from meat of goat serologically positive to the parasite. The infected meat was then fedinto uninfected cat to obtain oocyst. The oocyst was inoculated into the stomach of mice to produce tachyzoitwhich in turn produce cyst in tissue known as bradyzoit . The membrane protein was then isolated from thebradyzoit. The protein was analysed by sodium dodecyl sulfate electrophoresis (SDS-PAGE). The dataobtained were presented descriptively. The protein concentration isolated from each mouse infected at thedose of 1x107 oocysts was 11.91 mg. Two protein bands specific for bradyzoit were identified at 97.72 kDaand 67.60 kDa.
INTENSITAS CEMARAN LARVA NEMATODA GASTROINTESTINAL PADA RUMPUT GEMBALAAN TERNAK DOMBA DI ACEH BESAR Mufti Kamaruddin; Muhammad Hanafiah; Nurdianti -
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Telah dilakukan penelitian tentang intensitas cemaran larva nematoda dan jenis larva yang dominan pada rumput pengembalaan ternak domba di Aceh Besar. Sampel rumput diambil dari 4 desa, yaitu Meunasah Papeun, Lamreung, Leung Ie dan Cot Leupan. Pengambilan sampel dilakukan dengan membuat plot berbentuk huruf W pada sampel rumput yang akan diperiksa.
Gambaran Histopatologi Toksoplasmosis pada Kucing Peliharaan (HISTOPATHOLOGICAL FEATURES OF TOXOPLASMOSIS IN DOMESTIC CAT) Muhammad Hanafiah; Wisnu Nurcahyo; Joko Prastowo; Sri Hartati
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.81 KB) | DOI: 10.19087/jveteriner.2017.18.1.11

Abstract

Study of histopathological changes of domestic cat organs which were serologically positive toxoplasmosis and laboratory infected which Toxoplasma have been undertaken. Histological section is prepared from organs including brain, liver, lung, kidney, duodenum, jejunum, ileum, and spleen then stained using hematoxylin and eosin (HE) and observed under microscope for histopathological changes. The results showed that in the serologically positive animals cell proliferation, infiltration of leucocyte and macrophage cells were observed in the ileum, whilst infiltration of eosinophil and leucocyte was seen in the kidney and liver. However, in other organ such as duodenum, jejunum, and spleen there were no changes observed. In cat experimentally infected with Toxoplasma, the infiltration of eosinophil cells were observed in the ileum and lung, while other organs such as kidney, liver, brain, jejunum, duodenum, and spleen showed no infiltration of inflammation cells. In conclusion, based on the results seropositive cat, showed proliferation of epithelial cells, leucocyte cells, and macrophage cells in the ileum, while in the lung, kidney, and liver showed infiltration of eosinophil and leucocyte. No infiltration of inflammation cells were observed in the brain, jejunum, duodenum, and spleen. ABSTRAK Penelitian mengenai histopatologi beberapa organ kucing peliharaan yang positif Toxoplasma baik secara serologi maupun yang diinfeksikan telah dilakukan. Tujuan penelitian adalah untuk mengetahui perubahan histopatologi pada organ kucing yang positif Toxoplasma. Data hasil pemeriksaan histopatologi yang terdapat pada preparat jaringan masing-masing organ dianalisis secara deskriptif dengan melihat gambaran perubahan histopatologi pada organ otak, hati, paru, ginjal, duodenum, jejenum, ileum, dan limpa. Hasil penelitian menunjukkan bahwa dengan menggunakan metode histopatologi organ kucing yang positif toksoplasmosis secara serologi teramati adanya proliferasi sel epitel, infiltrasi sel-sel leukosit dan makrofag pada ileum, ginjal, dan hati terlihat adanya infiltrasi eosinofil dan juga infiltrasi leukosit, sedangkan organ yang lain seperti jejenum, duodenum, dan limpa tidak teramati perubahan pada jaringan yang diperiksa. Sementara pada kucing yang dinfeksikan Toxoplasma, ileum dan paru teramati adanya infiltrasi sel-sel eosinofil, sedangkan organ lainnya seperti ginjal, hati, otak, jejenum, duodenum, dan limpa tidak teramati adanya infiltrasi sel-sel radang. Simpulan yang dapat ditarik adalah pada organ kucing yang positif toksoplasmosis teramati adanya proliferasi sel epitel, infiltrasi sel-sel leukosit, dan makrofag pada ileum, paru, ginjal dan hati teramati adanya infiltrasi eosinofil dan juga infiltrasi leukosit, sedangkan organ-organ lainnya seperti otak, jejenum, duodenum dan limpa tidak terlihat adanya infiltrasi sel-sel radang.
Co-Authors Abd. Rasyid Syamsuri Adam S, Koji Al Amalia Sutriana arif gumilar Arman Sayuti Armia, Yasser Aulia Malik Fajar Aza Annisa Utami Baehaqi Basri A. Gani, Basri A. Dwinna Aliza Dwinna Aliza Emi Minarni Erdiansyah Rahmi Erdiansyah Rahmi Erizal Erizal Fadrial Karmil Farid Wajidi Farida Athaillah Farida Farida Farida Farida Hamdani Budiman Hamdani Budiman Ilham Ilham Indra Wahyudi, Indra Ismail Ismail Joko Prastowo Juli Melia M. Hasan Maulida, Sri Mudatsir Mudatsir Mufti Kamaruddin Mufti Kamaruddin Muhammad Bahi Muhammad Hambal Muhammad Hambal Muhammad Jalalluddin N. Nazaruddin Nur Afriyanti Nuraini Latif Nurdianti - NURLIANA NURLIANA Nurul Hidayati Nuzul Asmilia Pratama, Said Mirza R. Rusli Rastina Rastina Rusli . Rusli Rusli Rusli Sulaiman Salim, Muhammad Nur Sembiring, Rinawati Sri Hartati Sri Hartati Subhaini Jakfar Sugito Sugito Sugito Sugito Sukarni Sukarni Sulastri, S t fadrial karmil Teuku Armansyah Teuku Reza Ferasyi Teuku Reza Ferasyi Teuku Zahrial Helmi Widya Nanda Winaruddin Winaruddin Wisnu Nurcahyo Wisnu Nurcahyo Yudha Fahrimal Yundari, Yundari