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Journal : Research Journal of Life Science

Development of Candidate Antigens for Rapid Test Kit to Detect Autoantibodies in Patients with Systemic Lupus Erythematosus Wisnu Barlianto; Hani Susianti; Singgih Wahono; Nelly Ismayasih; Rossy Meilani; Kusworini Handono
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (693.941 KB) | DOI: 10.21776/ub.rjls.2017.004.01.8

Abstract

Systemic Lupus Erythematosus (LES) is an autoimmune inflammatory disease characterized by the formation of anti-nuclear antibodies (ANA) and anti-double stranded DNA (anti-dsDNA) antibodies as diagnostic markers. Detection of such autoantibodies requires advanced equipment and trained personnel.This study was conducted to acquire candidate antigens that can be used for rapid test kit for practical and accurate detection of ANA and anti-dsDNA to speed up SLE diagnosis.Nuclear proteins and DNA derived from cell lines, hair follicles, and leukocytes of SLE patients and healthy individuals were isolated using QiaGEN kit and modified-manual procedure. Antigen-antibody bonds were tested by dot blot assay.The strongest binding between DNA antigens of a healthy individual and antibodies occurred at dilution factors of 1:5,120 for the antigen and 1:2,560 for the antibody. The strongest binding between nuclear protein antigens from the cell line and antibodies occurred at dilution factors of 1:512 for the antigen and 1:1,600 for the antibody.Nuclear antigens derived from cell line and DNA antigens of healthy individuals were antigen candidates for the development of ANA and anti-dsDNA rapid detection tests.
Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus Nephritis Hani Susianti; Wisnu Barlianto; Dian Sukma Hanggara; Kusworini Handono; Purwanto Adipireno; Lisyani Suromo
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (875.828 KB) | DOI: 10.21776/ub.rjls.2017.004.01.6

Abstract

Background. Current biomarkers for evaluating disease activity or severity in lupus nephritis (LN) are considered to be unsatisfactory. Pathological changes in glomerular basement membrane and selectivity of electrical discharge are causing specific patterns of urine proteins excretion. Together with alpha-1 antitrypsin (AAT), they are expected to become new biomarkers to assess LN activity.Method. Seventy-one urine samples were collected from healthy controls and LN patients. Patterns of urine specific proteins were determined using column chromatography and SDS-PAGE tests, LN activity was calculated using SLEDAI-renal domain score, and AAT concentrations was measured by ELISA.Result. The majority of proteins in the control group have molecular weights of >66 kDa (88%) and 21- to 25-kDa proteins were observed only in the case group. The p values for differences in urine AAT concentration between active LN and healthy controls, inactive LN and healthy controls, and active LN and inactive LN were 0.004, 0.046, and 0.054, respectively, whereas those for urine AAT/creatinine ratio were 0.489, 0.019, and 0.915, respectively.Conclusion: There were differences in the patterns of the molecular weight of proteins and urine AAT concentrations between case group and control group. However, no such differences were identified between active and inactive LN. 
Co-Authors Abd. Rasyid Syamsuri Abdurrachman Abdurrachman Achmad Rifa’i, Achmad Achmad Rudijanto Achmad Rudijanto Agustina T Endharti, Agustina T Agustina Tri E Agustina Tri Endharti Ahmad Bayhaqi Nasir Aslam Airlangga, Dimas Ikhsan Albaar, Thoha M. Alfandy, Tommy N. Asih, Sari Wulan Ati Rastini Atma Gunawan Atma Gunawan Atma Gunawan Aulanni'am, Aulanni'am Balindra, Fredlina Rossa Bambang Rahardjo Benny A Pradana, Benny A Bowo Hery Prasetyo, Bowo Hery BP Putra Suryana BP Putra Suryana Cesarius Singgih Wahono Dalhar, Mochamad Daniel Happy Putra Dantara, Tri W.I. Dany Farida Desfryda, Elynca Putri Dessy Setiawati desy wulandari Dewi Purnama Sari Dewi, Elvira S. Dhani, Fauzan K. Dhian E. P. Harnandari Dian Ayu Wulansari, Dian Ayu Dian Hasanah Dian Sukma Hanggara Dimas Ikhsan Airlangga Djoko W. Soeatmadji Dona Marisa, Dona Dwinadella, Sephia Eko, Mudjiwijono Handaru Elvira Sari Dewi Ema Pristi Yunita Ema Pristi Yunita, Ema Pristi Engli, Katherina Enny Listyawati Erawati, Dini Rachma Erna Amin Faisal Faisal Farida ** Fatchiyah Fatchiyah Firdaningrum, Nimas Eka Gizta, Aura Bella Handono Kalim Hani Susanti, Hani Hani Susianti Hanik Ruliani Haribowo, A S Hidayat Sujuti HMS Chandra Kusuma I Putu A.S Ibrahim Njoto Ihda Dian Kusuma Indah Jayani Ivan A Hartono, Ivan A Janasti, Laksmitha Krisni Subandiyah Kurnianingsih, Nia L Enggar Fitri Laksmi Karunia Tanuwijaya Laksmi Karunia Tanuwijaya Lisyani Suromo Loeki Enggar Fitri M Rasjad Indra Machlusil Husna, Machlusil Maghfirah, Halimi Bidaimi Marsetyawan HNE Soesatyo Muhammad Anshory, Muhammad Muhammad Masyhur Nelly Ismayasih Nurdiana Nurdiana Nurhadi, Pradana Perdana Aditya Rahman Poetri, Levrita Nindya Pramadhani, Almira Prasetyo, Dwi A. Pratama, Mirza Z. Pratama, Mirza Zaka Purnomo, Athaya F. Purwanto Adipireno Putri, Choirinnisa Meilia Ayu Putu A. Herliawati R.I R.I Radhitio A Nugroho, Radhitio A Rahmawati, Hanifa Rizky Ria Famuji, Siti Roziah Rifa’i, A Rizky Fachry, Ade Wlidan Rossy Meilani Rulli Rosandi Safrina Dewi Ratnaningrum, Safrina Dewi Sari, Riana Trinovita Sari, Tita Luthfia Satrio Wibowo Singgih Wahono Solly Aryza Sri Andarini Indreswari Sri Poeranto, Sri Subandi Subandi Suharyo Hadisaputro Sulistomo, Hikmawan Wahyu Sulistyorini Sulistyorini Sumarno . Sumarta, Norma Hanifah Suryana, Bagus Putu Putra Sutrisno Sutrisno Suwito, Mat Syahrul Chilmi Tatit Nurseta Tatit Nurseta Torajasa Achamar Tri Yudani Mardining Raras VALENTINA YURINA VALENTINA YURINA Wahono, Caesarius Singgih Wahono, Cesarius S. Wahono, Cesarius Singgih Wisnu Barlianto Wisnu Barlianto Wisnu Barlianto Yahya Irwanto Yona One Sidarta, Yona One Yudhanto, Hendy Setyo Yuliana Salman Zaenal Kusuma