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GAMBARAN HISTOPATOLOGIS HEPATOPANKREAS UDANG WINDU (Penaeus monodon) AKIBAT INFEKSI VIRUS HEPATOPANCREATICA PARVOVIRUS (HPV) Nazaruddin N; Dwinna Aliza; Siti Aisyah; Zainuddin Z; Syafrizal S
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui gambaran histopatologis hepatopankreas udang windu yang terinfeksi hepatopancreatica parvovirus (HPV). Sebanyak 10 ekor udang windu yang berasal dari tambak rakyat digunakan sebagai sampel penelitian terdiri atas 5 sampel positif terinfeksi HPV dan 5 sampel normal. Semua udang dinekropsi untuk diambil hepatopankreas dan difiksasi dengan larutan Davidson. Selanjutnya hepatopankreas dibuat preparat histopatologis sesuai dengan prosedur teknik yang biasa dilakukan di Laboratorium Patologi Fakultas Kedokteran Hewan Universitas Syiah Kuala Darussalam Banda Aceh dan diwarnai dengan hematoksilin eosin (HE) serta diamati di bawah mikroskop. Hasil penelitian menunjukkan bahwa pada hepatopankreas udang windu yang terinfeksi HPV ditemukan adanya hipertrofi basofilik badan inklusi intranuklear, degenerasi, dan lisis sel.

STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)) Sri Wahyuni; Srihadi Agungpriyono; I. Ketut Mudite Adnyane; Hamny Hamny; Muhammad Jalaluddin; Gholib Gholib; Muslim Akmal; Mulyadi Adam; Dwinna Aliza; Tongku Nizwan Siregar
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 µg/ml of concentration for PNA lectins and 15µg/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis. Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak

THE EFFECT OF MALACCA LEAVES (Phyllantus emblica) ETHANOLIC EXTRACT ON Plasmodium falciparum GROWTH IN VITRO Nuzul Asmilia; T Armansyah TR; Dwinna Aliza; Juli Melia; Erdiansyah Rahmi; Lingga Surya Maret Daulay
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 µg/mL, 75 µg/mL, 50 µg/mL, 25 µg/mL, and 5 µg/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.25±15.62, 8.50±2.52, 8.50±3.00, 9.25±0.95, 9.00±2.70, 9.79±2.06, and 10.75±2.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.00±0.00%, 84.62±4.55%; 84.62±5.43%; 83.26±1.73%; 83.71±4,90%; 82.35±3,73%; and 80.54±6.83%, respectively (P0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 µg/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 µg/mL.

UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN MALAKA (Phyllantus emblica) TERHADAP MENCIT (Mus musculus). (Acute Toxicity Test of Ethanolic Extract of Malaka (Phyllantus emblica) Leaves on Mice (Mus musculus)) T. Armansyah TR; Sudi Indriany; Amalia Sutriana; Rosmaidar Rosmaidar; Nuzul Asmilia; Budianto Panjaitan; Dwinna Aliza; Hamdan Hamdan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

ABSTRACT The aim of this research was to asses the acute toxicity of ethanolic extract of malaka leaves using lethal dose 50 (LD50) on mice (Mus musculus). Twenty male mice weighing between 20-30 g were randomly divided into 4 groups (group K1-K4) of 5 mice each. All mice in group K1, K2, K3, and K4 were administered ethanolic extract of malaka leaves with the dose of 2, 4, 8, and 16 g/kg bw, respectively. Single dose of ethanolic extract of malaka leaves were given by oral gavage prior to clinical observation . The observation period was 14 days post administration, for sign of toxicity symptom, weight loss, and mortality. The result showed that no mortality was observed in the experimental animals during this study. Slight reduction of body weight was observed in group K2, K3, and K4, and no toxicity sign was found during fourteen days of observation. The LD50 of ethanolic extract of malaka leaves was higher than 16 g/kg body weight, thus, the substance was practically non toxic substance.

Characterization of Inhibin from Culture and Non Culture of Granulose Cells for Monoclonal Antibody of Inhibin Production Amiruddin Amiruddin; Tongku Nizwan Siregar; Amalia Sutriana; Dwinna Aliza; T. Armansyah
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

This study has long-term objectives to obtain immunogenic prototype that can be used to induce multiple ovulation in goats. Working steps of this study were begun with the collection of ovarium from goats, collection of granulose cells, culture of granulose and characterization of molecular weight and isoelectric point (pI) of inhibin protein of granulose cells obtained from culture and non-culture of granulose cells, and followed by preparation of monoclonal antibody toward inhibin. The results showed that inhibin isolated either from culture or non-culture of granulose cells produced a 32 kDa band. Molecular weight of inhibin was measured by Western Blot. The 32 kDa band of SDS PAGE product appeared on Western Blot result was inhibin molecules produced by granulose cells collected fom culture and non-culture of granulose cells that can be identified by Mab-inhibin. Product of IEF gel electrophoresis suggested that inhibin molecule collected from culture of granulose cells has no charge at isoelectric points ranging from 5-6, depends on its total amino acid composition.

Characterization of Inhibin from Culture and Non Culture of Granulose Cells for Monoclonal Antibody of Inhibin Production Amiruddin Amiruddin; Tongku Nizwan Siregar; Amalia Sutriana; Dwinna Aliza; T. Armansyah
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9789

This study has long-term objectives to obtain immunogenic prototype that can be used to induce multiple ovulation in goats. Working steps of this study were begun with the collection of ovarium from goats, collection of granulose cells, culture of granulose and characterization of molecular weight and isoelectric point (pI) of inhibin protein of granulose cells obtained from culture and non-culture of granulose cells, and followed by preparation of monoclonal antibody toward inhibin. The results showed that inhibin isolated either from culture or non-culture of granulose cells produced a 32 kDa band. Molecular weight of inhibin was measured by Western Blot. The 32 kDa band of SDS PAGE product appeared on Western Blot result was inhibin molecules produced by granulose cells collected fom culture and non-culture of granulose cells that can be identified by Mab-inhibin. Product of IEF gel electrophoresis suggested that inhibin molecule collected from culture of granulose cells has no charge at isoelectric points ranging from 5-6, depends on its total amino acid composition.

IDENTIFICATION OF COPPER-INDUCIBLE GENES IN SWORDTAIL FISH (Xiphophorus spp.) USING DIFFERENTIAL DISPLAY Dwinna Aliza; Tengku Muhammad
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i1.3498

Differential display technique was used to identify differentially expressed gene ofswordtail fish (Xiphophorus spp.) that induced by 1 µg/ml copper for 24 hours. From eightprimers that have been used for differential display, seven of them showed the differentialgenes which were not present in the control. The reamplification of these fragments rangingfrom below 100 to 200 bp. But only two fragments were successfully cloned and sequenced,those are H-AP10 and H-AP11. Sequence analysis showed that both of the sequences revealedhigh similarity with Homo sapiens stress-associated endoplasmic reticulum protein, a signalgenerated that induces apoptosis.Keywords: differential display, swordtail fish, copper pollution, mRNA expression, biomarker

Effect of Storage Time of Epididymis at 5 oC on Spermatozoa Quality of Aceh Local Goat Hamdan Hamdan; Budianto Panjaitan; Amalia Sutriana; Dwinna Aliza; Erdiansyah Rahmi
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9811

The objective of this study was to evaluate the effect of storage time of epididymis at 5 oC on spermatozoa quality of Aceh local goat. This research was conducted at Reproduction Laboratory, FKH Unsyiah from May to June 2009. Nine epididymis from local goat at the age of 1.5-2.0 years were used in this study. The epididymis were collected from abattoir in Banda Aceh. The experimental design used in this study was completely randomized design with 3 treatments and 3 replications. Spermatozoa quality which consist of sperm concentration, motility, amount of life sperm, and sperm abnormality were examined after the collection of epididymis on day 0 (H-0), day 1 (H-1), and day 3 (H-3) post storage at 5 oC. Data were analyzed using one-way analyses of variance (ANOVA). The result showed that no significant difference (P0.05) seen in the spermatozoa quality after different storage time. The average of spermatozoa concentration on H-0, H-1, and H-3 were 318x107/ml, 282x107/ml, and 241x107/ml respectively. On the average, the percentage of spermatozoa motility on H-0, H-1, and H-3 were 82,27Â±2,75; 80,25Â±2,83; and 78,07Â±0,92%, respectively. Life spermatozoa observed on H-0, H-1, and H-3 were 82,29Â±2,71; 80,63Â±1,87; and 80,09Â±3,31%, respectively. Observation on spermatozoa abnormality showed that the average of spermatozoa abnormality on H-0, H-1, and H-3 were 7,23Â±0,27; 8,21Â±0,55; and 10,75Â±3,14%, respectively. It could be concluded that the spermatozoa quality were not affected by the storage time at temperature of 5 oC.

DIFERENSIAL LEUKOSIT DAN KETAHANAN HIDUP PADA UJI TANTANG Aeromonas hydrophila IKAN NILA YANG DIBERI STRES PANAS DAN SUPLEMENTASI TEPUNG DAUN JALOH DALAM PAKAN Sugito S; Nurliana N; Dwinna Aliza; Samadi S
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2652

Penelitian ini dilakukan untuk mengetahui efek suplementasi tepung daun jaloh (Salix tetrasperma Roxb) (TDJ) dalam pakan terhadap nilai diferensial leukosit dan ketahanan hidup setelah uji tantang dengan Aeromonas hydrophila pada ikan nila (Oreochromis niloticus) yang dipelihara pada akuarium diberi peningkatan suhu lingkungan. Penelitian ini menggunakan rancangan acak lengkap faktorial 2x4. Faktor pertama adalah penambahan jumlah tepung daun jaloh dalam pakan yaitu: 0% (P1), 5% (P2), 10% (P3), dan 15% (P4) dari berat pakan dan faktor kedua adalah suhu air dalam akuarium yaitu 29Â±1ï‚° C (S1) dan 35Â±1ï‚° C (S2), sehingga didapat 8 kombinasi perlakuan yaitu: P1S1, P2S1, P3S1, P4S1, P1S2, P2S2, P3S2, dan P4S2 dengan ulangan 10 ekor ikan per perlakuan. Sebanyak 80 ekor ikan nila dengan bobot badan 40-50 g secara acak dibagi ke dalam 8 perlakuan. Perlakuan dilakukan selama 30 hari. Pada hari ke 31 dilakukan pengambilan sampel darah dan uji tantang. Hasil penelitian ini menunjukkan bahwa suplementasi pakan dengan TDJ pada konsentrasi 5-15% tidak berpengaruh terhadap nilai diferensial leukosit ikan nila yang dipelihara pada suhu lingkungan yang berbeda. Suplementasi TDJ dalam pakan sebanyak 5-10% dapat mengurangi kematian ikan akibat infeksi Aeromonas hydrophila terutama pada suhu 35Â±1ï‚° C. Dari hasil penelitian ini dapat disimpulkan bahwa ikan nila yang dipelihara pada akuarium dengan suhu air 35Â±1ï‚° C dan diberi pakan yang disuplementasi TDJ 5-10% dapat meningkatkan daya tahan tubuh ikan nila.

Histopathological Changes of Erythrocyte and Quantity of immature Erythrocytes in Tegal Duckling (Anas Javanica) Induced by Lead (Pb) Hamdani Budiman; Dwinna Aliza
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9792

The aim of this research is to find out the histopathological changes of erythrocyte and the quantity of immature erythrocyte of tegal duckling treated with lead (Pb). Twenty five young female ducks with the age of 7 days and 60.55.07 g body weight alloted into 4 treatment groups and 1 control group (Ko) with 5 replication each. The treatment group fed with K1 (5.7), K2 (11.4), K3 (17.1), and K4 (22.8) mg Pb/kg body weight for 10 days, respectively. Young ducks showed clinical symptoms such as the color of feces were brown to black, green color of diarrhea, anemic and decreasing body weight. Histopathologically, erythrocyte showed that immature and abnormal in shape (like teardrops, dumb bell, and bottles). The result of variant analysis followed by linier regression shows that the higher the dosage of Pb in diet the higher the quantity of immature erythrocyte andabnormal erythrocyte shape found (P0.01).

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