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All Journal Jurnal Veteriner Jurnal Ilmiah Mahasiswa Veteriner Jurnal Kedokteran Hewan Jurnal Medika Veterinaria Jurnal Agripet

Dwinna Aliza

Faculty Of Veterinary Medicine, Universitas Syiah Kuala

Author-ID : 2751520

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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GAMBARAN HISTOPATOLOGIS HEPATOPANKREAS UDANG WINDU (Penaeus monodon) AKIBAT INFEKSI VIRUS HEPATOPANCREATICA PARVOVIRUS (HPV) Nazaruddin N; Dwinna Aliza; Siti Aisyah; Zainuddin Z; Syafrizal S
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui gambaran histopatologis hepatopankreas udang windu yang terinfeksi hepatopancreatica parvovirus (HPV). Sebanyak 10 ekor udang windu yang berasal dari tambak rakyat digunakan sebagai sampel penelitian terdiri atas 5 sampel positif terinfeksi HPV dan 5 sampel normal. Semua udang dinekropsi untuk diambil hepatopankreas dan difiksasi dengan larutan Davidson. Selanjutnya hepatopankreas dibuat preparat histopatologis sesuai dengan prosedur teknik yang biasa dilakukan di Laboratorium Patologi Fakultas Kedokteran Hewan Universitas Syiah Kuala Darussalam Banda Aceh dan diwarnai dengan hematoksilin eosin (HE) serta diamati di bawah mikroskop. Hasil penelitian menunjukkan bahwa pada hepatopankreas udang windu yang terinfeksi HPV ditemukan adanya hipertrofi basofilik badan inklusi intranuklear, degenerasi, dan lisis sel.

STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)) Sri Wahyuni; Srihadi Agungpriyono; I. Ketut Mudite Adnyane; Hamny Hamny; Muhammad Jalaluddin; Gholib Gholib; Muslim Akmal; Mulyadi Adam; Dwinna Aliza; Tongku Nizwan Siregar
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 µg/ml of concentration for PNA lectins and 15µg/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis. Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak

THE EFFECT OF MALACCA LEAVES (Phyllantus emblica) ETHANOLIC EXTRACT ON Plasmodium falciparum GROWTH IN VITRO Nuzul Asmilia; T Armansyah TR; Dwinna Aliza; Juli Melia; Erdiansyah Rahmi; Lingga Surya Maret Daulay
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 µg/mL, 75 µg/mL, 50 µg/mL, 25 µg/mL, and 5 µg/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.25±15.62, 8.50±2.52, 8.50±3.00, 9.25±0.95, 9.00±2.70, 9.79±2.06, and 10.75±2.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.00±0.00%, 84.62±4.55%; 84.62±5.43%; 83.26±1.73%; 83.71±4,90%; 82.35±3,73%; and 80.54±6.83%, respectively (P0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 µg/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 µg/mL.

UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN MALAKA (Phyllantus emblica) TERHADAP MENCIT (Mus musculus). (Acute Toxicity Test of Ethanolic Extract of Malaka (Phyllantus emblica) Leaves on Mice (Mus musculus)) T. Armansyah TR; Sudi Indriany; Amalia Sutriana; Rosmaidar Rosmaidar; Nuzul Asmilia; Budianto Panjaitan; Dwinna Aliza; Hamdan Hamdan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

ABSTRACT The aim of this research was to asses the acute toxicity of ethanolic extract of malaka leaves using lethal dose 50 (LD50) on mice (Mus musculus). Twenty male mice weighing between 20-30 g were randomly divided into 4 groups (group K1-K4) of 5 mice each. All mice in group K1, K2, K3, and K4 were administered ethanolic extract of malaka leaves with the dose of 2, 4, 8, and 16 g/kg bw, respectively. Single dose of ethanolic extract of malaka leaves were given by oral gavage prior to clinical observation . The observation period was 14 days post administration, for sign of toxicity symptom, weight loss, and mortality. The result showed that no mortality was observed in the experimental animals during this study. Slight reduction of body weight was observed in group K2, K3, and K4, and no toxicity sign was found during fourteen days of observation. The LD50 of ethanolic extract of malaka leaves was higher than 16 g/kg body weight, thus, the substance was practically non toxic substance.

Characterization of Inhibin from Culture and Non Culture of Granulose Cells for Monoclonal Antibody of Inhibin Production Amiruddin Amiruddin; Tongku Nizwan Siregar; Amalia Sutriana; Dwinna Aliza; T. Armansyah
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

This study has long-term objectives to obtain immunogenic prototype that can be used to induce multiple ovulation in goats. Working steps of this study were begun with the collection of ovarium from goats, collection of granulose cells, culture of granulose and characterization of molecular weight and isoelectric point (pI) of inhibin protein of granulose cells obtained from culture and non-culture of granulose cells, and followed by preparation of monoclonal antibody toward inhibin. The results showed that inhibin isolated either from culture or non-culture of granulose cells produced a 32 kDa band. Molecular weight of inhibin was measured by Western Blot. The 32 kDa band of SDS PAGE product appeared on Western Blot result was inhibin molecules produced by granulose cells collected fom culture and non-culture of granulose cells that can be identified by Mab-inhibin. Product of IEF gel electrophoresis suggested that inhibin molecule collected from culture of granulose cells has no charge at isoelectric points ranging from 5-6, depends on its total amino acid composition.

Behavior and Gill profile of Nile Tilapia (Oreochromis niloticus) Induced by Density stress Dwinna Aliza
Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v8i1.3344

The aim of this research was to observe behavior and gill profile of nile tilaphia (Oreochromis niloticus) induced by density stress. A total of 50 nile tilapia fish with the age of 3-8 months and 80-100 bwwere divided into 4 groups. Group I was control group contained 5 fish, group II,III, and IV contained 10, 15, and 20 fish, respectively. Treatment was performed for 24 hours, and fish behavior was observed every 3 hours. Gillsample was examined by anatomical pathology then tissue samples were collected and fixed in 10% Davidson solution, routinely processed,embedded in paraffin, sectioned at 5 microns, stained with hematoxylin and eosin (HE), and examined by light microscopy. The data wasanalyzed descriptively. Behavior changessuch as swim up to water surface and rapid movement of operculum occur earlier than passivemovement and decreased reflex of fish on group II, III, and IV. The anatomical pathology of nile tilapia gills observed were darker colour ofgills, gill rott, and over mucus.Histopathological result showed that haematoma, necrosis, hyperplasia of primary and secondary lamella, andfusion lamella were observed in gills of nile tilapia induced by density stress.____________________________________________________________________________________________________________________Key words: histopathology, nile tilapia, gill, density stress

26. GAMBARAN HISTOPATOLOGIS GINJAL TIKUS PUTIH (Rattus novergicus) JANTAN YANG DIINFEKSIKAN Trypanosoma evansi DAN DIBERI EKSTRAK DAUN SERNAI (Wedelia biflora) (Histopathology of Male Rat (Rattus novergicus) Kidney Infected with Trypanosoma evansi and Treated with Sernai Leaves Extract (Wedelia biflora)) Yudha Fahrimal; Rahmiwati R; Dwinna Aliza
Jurnal Medika Veterinaria Vol 10, No 2 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i2.4386

This research was aimed to study histopathology of rat kidney infected with Trypanosoma evansi after administration of sernai leaves extract (Wedelia bifolora). The samples used were kidneys of 25 male rats strain Wistar which were equally divided into 5 treatment groups. Group P1 was uninfected and untreated control, group P2 was infected with 1x103 T. evansi but not sernai leaves extract, rats from groups P3, P4, and P5 were infected with 1x103 T. evansi and given sernai leaves extract with the dose of 30 mg/kg bw, 45 mg/kg bw, and 60 mg/kg bw respectively. The sernai extract was administered orally using a stomach tube for 3 consecutive days. The next day after last treatment, all rats were sacrificed and necropsied. Kidneys were collected for histopathological examination such as degeneration and necrosis of tubules cells, convoluted contortus duct lumen, and adhesion and atrophy of glomerulus. Sernai leaves extract with the dose of 45 mg/kg bw was the most effective dose to prevent kidney damage due to T. evansi infection.

9. The Effectiviteness of Jatropha salve (Jatropha curcas L) in Inflammatory Phase of cutaneous wound healing in Mice (Mus musculus) Histopathologically Indra Sitorus; M Nur Salim; Nazaruddin Nazaruddin; Dwinna Aliza; Denny Irmawati; Awaluddin Awaluddin; Cut Nila Thasmi; Muslim Akmal; Dian Masyitha
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.4374

This study aims to determine the effectiveness of 10% Jatropha salve (Jatropha curcas L. ) to inflammation reaction cutaneous wound healing in mice (Mus musculus ) in the inflammatory phase. This experiment used 9 male mices, weighing 25-40 grams and age 2-3 months old, were divided into three treatment groups, each treatment consisting of three mices. The wound were made along 2 cms in the back area. Wound therapy was carried out twice a day for 3 days. Group KI was administered and given by yellow vaseline, KII was given 10% Jatropha salve, and KIII was given gentamicin salve. The parameters measured are the increase in the number infiltrations of inflammatory cells, neovascular, and re-epitelization. All quantitative data were measured using ANOVA and continued with Duncan Test, the qualitative data were presented descriptively by showing the results of observation under a microscope. The statistical test on the infiltrations of inflammatory cells and neovasculars parameter 10% Jatropha salve group significantly different (P0.05) compared to KI and KIII group. Histopatologycal observations indicated the 10% Jatropha salve could be reduce the infiltrations of inflammatory cells, increase neovasculars and re-epitelization percentage. According to the result can be concluded the 10% Jatropha salve can accelerate the inflammation phase cutaneous wound healing in mice.

35. The distribution of melanomacrophage center in liver and kidney of Tilapia fish that infected by Aeromonas hydrophilic Dian Pratiwi; Dwinna Aliza; Nazarudin Nazarudin; Zuhrawati Zuhrawati; Hamdani Budiman; Razali Daud
Jurnal Medika Veterinaria Vol 13, No 2 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i2.3648

This research aims was to determine the number of melanomacrophage center (MMC) in the liver and kidney of tilapia (Oreochromis niloticus) were injected A.hydrophila. This research uses 15 tilapia fish with an average weight of 200-300 g were divided into 5 groups, the control group, and four treatment groups. All treatment groups were injected bacteria A. hydrophila 10-5 CFU dose as much as 0.01 ml intramuscularly. Kidney and liver of tilapia in the control group and K1 were collected on day 1 post-injection, K2 on day 2 post-injection, K3 on day 3 post-injection, K4 on day 4 post-injection, and K5 on day 5 post-injection. MMC Joomla Data were analyzed by ANOVA. Results of the study group K0, K1, K2, K3, K4, and K5 in the liver was 20 ± 18.19 K0, K1 55.67 ± 38.81, 69.67 ± 18.19 K2, K3 80.33 ± 6, 42, 84 ± 6 K4, K5 88.33 ± 4.9. While the results of the kidneys was 32 ± 4.0 K0, K1 57 ± 41.9, 112.3 ± 16.4 K2, K3 113.3 ± 15.3, 96.3 ± 13.5 K4, K5 119.6 ± 30.4. Conclusion The infection of the longer period the higher the number of MMC.

PENGARUH UMUR TERHADAP PENJANTANAN LARVA IKAN LELE DUMBO (Clarias gariepinus) YANG DIRENDAM MENGGUNAKAN HORMON METIL TESTOTERON ALAMI (The Influence of Age on Masculinization of Clarias gariepinus Larvae Immersed by Methyl Testosterone Natural Hormone) Hattanul Mulia; Rosmaidar Rosmaidar; Dasrul Dasrul; Dwinna Aliza; Dian Masyitha; Sugito Sugito
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i1.4036

This research aims to examines the influence of age on masculinization of Clarias gariepinus larvae immersed by methyl testosterone (MT) natural hormone. This research used 270 catfish larvae divided into 3 groups of age those are 5, 10, and 20 days. The research implemented complete random design (CRD). Immersion was performed using methyl testosterone hormone at the dose of 0.25 g/L for 20 hours. Observation of fish was done after 60 day-old fish. Data obtained was analyzed using one-way analysis of variance The average percentages of male-sex catfish after immersing in natural MT on group I, II, and III were 97.67, 91.07, and 86.67%, respectively. The results showed that the age of the larvae did not affect significantly (P0.05) on the percentage of male sex of Clarias gariepinus. The average percentage of male sex in 5 days age group was higher than 10 and 20 days age group. In conclusion, the masculinization of Clarias gariepinus larva immersed by MT natural hormone is not influenced by age of fish before dipping.

Co-Authors . Nurliana Abdullah A. Muhammadar Aisyah Fadillah Tunnisa Alfin Oktarian Amalia Sutriana Amiruddin Amiruddin Arman Sayuti Asmawati Asmawati Awaluddin Awaluddin Bagus Dwijayanti Batubara, Agung Setia Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan chiara giri puspa Cut Nila Thasmi Dasrul Dasrul Dedi Fazriansyah Putra Denny Irmawati Dian Masyitha Dian Pratiwi Erdiansyah Rahmi Erdiansyah Rahmi Fadli A. Gani Firdus Firdus Fitriani Fitriani Gholib Gholib Hafizuddin Hafizuddin Hafizuddin Hafizuddin Hamdan Hamdan Hamdani Budiman Hamdani Budiman Hamny Sofyan Hattanul Mulia Indra Sitorus Jesica Ramadhanita Juli Melia Ketut Adnyane Mudite Lingga Surya Maret Daulay M Nur Salim Mahdi Abrar Mira Ayu Lestari Hasibuan Muchlisin Zainal Abidin Muhammad Hanafiah Muhammad Jalaluddin Mulyadi Adam Muslim Akmal Nazaruddin N Nazarudin Nazarudin Nellita Meutia Nurhazimah Nurhazimah Nuzul Asmilia Rahmiwati R Rastina Rastina Razali Daud Roslizawaty Roslizawaty Rosmaidar Rosmaidar Samadi S Siti Aisyah Srihadi Agungpriyono Sudi Indriany Sugito Sugito Syafrizal S Syafruddin Syafruddin T Reza Ferasyi T. Armansyah T. Armansyah Tengku Muhammad Teuku Armansyah Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Ummu Balqis Wahyuni, Sri Yudha Fahrimal Zainuddin Zainuddin Zuhrawati Zuhrawati

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