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All Journal Jurnal Veteriner Jurnal Ilmiah Mahasiswa Veteriner Jurnal Kedokteran Hewan Jurnal Medika Veterinaria Jurnal Agripet
Dwinna Aliza
Faculty Of Veterinary Medicine, Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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EFFECT OF SALINITY CHANGES ON METHIONINE CONTENT IN TIGER GROUPER JUVENILE (Epinephelus fuscoguttatus) Muhammadar Abdullah Abbas; Muchlisin Zainal Abidin; Dedi Fazriansyah Putra; Firdus Firdus; Agung Setia Batubara; Asmawati Muhammad Sail; Syafruddin Syafruddin; Roslizawaty Roslizawaty; Amalia Sutriana; Dwinna Aliza
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.12363

Abstract

This study was conducted to determine the effect of water salinity changes on methionine content in tiger grouper juvenile (Epinephelus fuscoguttatus). A total of 2,560 tiger grouper juveniles were used and divided into five groups consist of 1 control group (without exposed to salinity changes) and 4 treatment groups. The salinity was changed every 2, 3, 4, and 6 hours in treatment A, B, C, and D, respectively. Salinity levels were changed during 24 hours by lowering salinity level from 32 psu to 22 psu. Twenty five of fish were collected from each treatment for methionine content analysis. Data were analysed using one way analysis of variance (ANOVA). The results showed that there was a decrease in methionine content in all treatments. A significant decrease (P0.05) of methionine content in treatment A, B, and C were observed after 20 hours (1.15%), 18 hours (1.27%), and 16 hours (1.24%), respectively. While at 0 hours (control), the methionine content was 2.02%. Methionine level in treatment D was not significantly different (P0.05) compared to control group. As conclusion, rearing the tiger grouper juvenile with salinity fluctuations every 6 hours did not lead to methionine deficiency.
IDENTIFIKASI PROTEIN IMUNOGENIK TAKIZOIT DAN BRADIZOIT TOXOPLASMA GONDII STRAIN LOKAL UNTUK PENGEMBANGAN KIT DIAGNOSTIK Muhammad Hanafiah; Dwinna Aliza
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.559

Abstract

Telah dilakukan penelitian untuk mengisolasi dan mengidentifikasi protein 28 kDa (GRA2) dari stadium takizoit dan bradizoit Toxoplasma gondii strain lokal. Cairan asites mencit galur Balb/c digunakan sebagai protein sampel melalui elektroforesis (SDS-PAGE), serum kambing untuk uji antigenitas protein 28 kDa (GRA2) dan serum darah kucing untuk melihat tingkat sensitivitas dan spesifisitas protein 28 kDa (GRA2). Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa protein 28 KDa (GRA2) telah dapat diisolasi dari stadium takizoit dan bradizoit Toxoplasma sp. Nilai OD405 antigenesitas ES adalah sebesar 0,578 sedangkan pada antigen GRA2 (28 kDa) diperoleh nilai OD405 adalah sebesar 0,178. Tingkat sensitivitas protein 28 kDa (GRA2) terhadap antibodi dalam serum darah kucing terinfeksi adalah sebesar 61,53% dan nilai spesifisitasnya adalah 33,33%. Tingkat sensitivitas protein ES terhadap antibodi dalam serum darah kucing terinfeksi adalah 76,92% dan tingkat spesifisitasnya adalah 33,33%.
ACEH CATTLE FOLLICLE DYNAMIC UNDER ENVIRONMENTAL HEAT STRESS Teuku Armansyah; Tongku Nizwan Siregar; Dwinna Aliza; Juli Melia; Nellita Meutia; Budianto Panjaitan; Hafizuddin Hafizuddin; Mulyadi Adam; Mahdi Abrar
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.6377

Abstract

The objective of this research is to know Aceh cattle follicle dynamic under environmental heat stress condition. This research was conducted on December 2012 until August 2013. Animals used in this research were 20 Aceh cattle aged 5-8 years old, weighing of 150-250 kg, and had at least 2 regular cycles. All cattle used were clinically in good body condition score. The cattle were divided into two groups of 10, used in two different period of times (December-January and July-August) to examine the effect of environmental heat to their follicle dynamic. Each group is separated into two different keeping management; one group was kept in pens while the others in the pasture, 5 cattle in each management group. Research timing was based on information acquired from Indrapuri Agency for Meteorology, Climatology, and Geophysics (BMKG) which predicted that extreme weather would last from July to August while December to January would be relatively normal in Aceh region. All cattle’s estrous cycle were synchronized by 5 mg/mL of PGF2α intramuscularly (Lutalyse™, Pharmacia Upjohn Company, Pfizer Inc.). Ultrasonography examination was performed to monitor ovary follicle’s growth and dynamic during one cycle. Days during ovulation marked by standing heat was regarded as Day 0 of estrous cycle. Follicular dynamic examination during estrous cycle on December-January and also on July-August, both penned or pastured cattle showed the follicle waves that was 3 follicle growth waves. The size of follicle growth on first wave (1st DF) on cattle kept in pen on July-August and December-January were relatively similar. The size of follicle growth on first wave for cattle kept in pasture on July-August and December-January were relatively similar. To conclude, Aceh cattle’s follicle dynamic is not change during environment heat stress condition and is not affected by different cattle-keeping management.
GAMBARAN HISTOPATOLOGIS HEPATOPANKREAS UDANG WINDU (Penaeus monodon) AKIBAT INFEKSI VIRUS HEPATOPANCREATICA PARVOVIRUS (HPV) Nazaruddin N; Dwinna Aliza; Siti Aisyah; Zainuddin Z; Syafrizal S
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1251

Abstract

Penelitian ini bertujuan mengetahui gambaran histopatologis hepatopankreas udang windu yang terinfeksi hepatopancreatica parvovirus (HPV). Sebanyak 10 ekor udang windu yang berasal dari tambak rakyat digunakan sebagai sampel penelitian terdiri atas 5 sampel positif terinfeksi HPV dan 5 sampel normal. Semua udang dinekropsi untuk diambil hepatopankreas dan difiksasi dengan larutan Davidson. Selanjutnya hepatopankreas dibuat preparat histopatologis sesuai dengan prosedur teknik yang biasa dilakukan di Laboratorium Patologi Fakultas Kedokteran Hewan Universitas Syiah Kuala Darussalam Banda Aceh dan diwarnai dengan hematoksilin eosin (HE) serta diamati di bawah mikroskop. Hasil penelitian menunjukkan bahwa pada hepatopankreas udang windu yang terinfeksi HPV ditemukan adanya hipertrofi basofilik badan inklusi intranuklear, degenerasi, dan lisis sel.
STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)) Sri Wahyuni; Srihadi Agungpriyono; I. Ketut Mudite Adnyane; Hamny Hamny; Muhammad Jalaluddin; Gholib Gholib; Muslim Akmal; Mulyadi Adam; Dwinna Aliza; Tongku Nizwan Siregar
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3396

Abstract

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 µg/ml of concentration for PNA lectins and 15µg/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis. Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak
THE EFFECT OF MALACCA LEAVES (Phyllantus emblica) ETHANOLIC EXTRACT ON Plasmodium falciparum GROWTH IN VITRO Nuzul Asmilia; T Armansyah TR; Dwinna Aliza; Juli Melia; Erdiansyah Rahmi; Lingga Surya Maret Daulay
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.10215

Abstract

The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 µg/mL, 75 µg/mL, 50 µg/mL, 25 µg/mL, and 5 µg/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.25±15.62, 8.50±2.52, 8.50±3.00, 9.25±0.95, 9.00±2.70, 9.79±2.06, and 10.75±2.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.00±0.00%, 84.62±4.55%; 84.62±5.43%; 83.26±1.73%; 83.71±4,90%; 82.35±3,73%; and 80.54±6.83%, respectively (P0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 µg/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 µg/mL.
UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN MALAKA (Phyllantus emblica) TERHADAP MENCIT (Mus musculus). (Acute Toxicity Test of Ethanolic Extract of Malaka (Phyllantus emblica) Leaves on Mice (Mus musculus)) T. Armansyah TR; Sudi Indriany; Amalia Sutriana; Rosmaidar Rosmaidar; Nuzul Asmilia; Budianto Panjaitan; Dwinna Aliza; Hamdan Hamdan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5137

Abstract

ABSTRACT The aim of this research was to asses the acute toxicity of ethanolic extract of malaka leaves using lethal dose 50 (LD50) on mice (Mus musculus). Twenty male mice weighing between 20-30 g were randomly divided into 4 groups (group K1-K4) of 5 mice each. All mice in group K1, K2, K3, and K4 were administered ethanolic extract of malaka leaves with the dose of 2, 4, 8, and 16 g/kg bw, respectively. Single dose of ethanolic extract of malaka leaves were given by oral gavage prior to clinical observation . The observation period was 14 days post administration, for sign of toxicity symptom, weight loss, and mortality. The result showed that no mortality was observed in the experimental animals during this study. Slight reduction of body weight was observed in group K2, K3, and K4, and no toxicity sign was found during fourteen days of observation. The LD50 of ethanolic extract of malaka leaves was higher than 16 g/kg body weight, thus, the substance was practically non toxic substance.
Histopathologic Features of Trachea and Lungs in Chickens with Chronic Respiratory Disease Aiza Annisa; Darmawi Darmawi; Etriwati Etriwati; Ummu Balqis; M. Nur Salim; Nazaruddin Nazaruddin; Dwinna Aliza; Siti Aisyah; Awaluddin Awaluddin; Denny Irmawati Hasan; Muslim Akmal; T. Zahrial Helmi; Nuzul Asmilia
Jurnal Medika Veterinaria Vol 18, No 1 (2024): J.Med.Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v18i1.37429

Abstract

The purpose of this case study is to determine the changes that exist in chickens infected with Chronic Respiratory disease (CRD). A broiler male chicken aged ± 40 days was found dead in a chicken basket at Ulee Kareng chicken slaughterhouse in a state of feather loss and feces attached to cloaca, on January 6th, 2023. The chicken was brought to pathology laboratory of Faculty of Veterinary Medicine, Universitas Syiah Kuala for necropsy and organ collection. Organs associated with CRD cases were trachea and lungs and histopathological preparations were carried out, stained with Haematoxylin Eosin, and observed under a microscope. The results of histopathological examination showed hemorrhage and hyperplasia of the tracheal epithelium. Hemorrhage was caused by edema which causes increased vascular permeability and epithelial cell hyperplasia occured due to the body's response to infection or inflammation. In the lungs there was bleeding due to damage to the blood vessel wall which caused increased vascular permeability, so that fluid and blood plasma come out of the blood vessels. Fibrinogen then underwent polymerization which resulted in fibrinous thrombi. Congestion happened as the result of fluid accumulation in a location that occured in a passive process so the fluid failed to escape. Because of the accumulation of fluid (congestion), the blood supply to the lung tissue is interrupted, resulting in several points of necrosis in the lungs.
Behavior and Gill profile of Nile Tilapia (Oreochromis niloticus) Induced by Density stress Dwinna Aliza
Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v8i1.3344

Abstract

 The aim of this research was to observe behavior and gill profile of nile tilaphia (Oreochromis niloticus) induced by density stress. A total of 50 nile tilapia fish with the age of 3-8 months and 80-100 bwwere divided into 4 groups. Group I was control group contained 5 fish, group II,III, and IV contained 10, 15, and 20 fish, respectively. Treatment was performed for 24 hours, and fish behavior was observed every 3 hours. Gillsample was examined by anatomical pathology then tissue samples were collected and fixed in 10% Davidson solution, routinely processed,embedded in paraffin, sectioned at 5 microns, stained with hematoxylin and eosin (HE), and examined by light microscopy. The data wasanalyzed descriptively. Behavior changessuch as swim up to water surface and rapid movement of operculum occur earlier than passivemovement and decreased reflex of fish on group II, III, and IV. The anatomical pathology of nile tilapia gills observed were darker colour ofgills, gill rott, and over mucus.Histopathological result showed that haematoma, necrosis, hyperplasia of primary and secondary lamella, andfusion lamella were observed in gills of nile tilapia induced by density stress.____________________________________________________________________________________________________________________Key words: histopathology, nile tilapia, gill, density stress 
26. GAMBARAN HISTOPATOLOGIS GINJAL TIKUS PUTIH (Rattus novergicus) JANTAN YANG DIINFEKSIKAN Trypanosoma evansi DAN DIBERI EKSTRAK DAUN SERNAI (Wedelia biflora) (Histopathology of Male Rat (Rattus novergicus) Kidney Infected with Trypanosoma evansi and Treated with Sernai Leaves Extract (Wedelia biflora)) Yudha Fahrimal; Rahmiwati R; Dwinna Aliza
Jurnal Medika Veterinaria Vol 10, No 2 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i2.4386

Abstract

This research was aimed to study histopathology of rat kidney infected with Trypanosoma evansi after administration of sernai leaves extract (Wedelia bifolora). The samples used were kidneys of 25 male rats strain Wistar which were equally divided into 5 treatment groups. Group P1 was uninfected and untreated control, group P2 was infected with 1x103 T. evansi but not sernai leaves extract, rats from groups P3, P4, and P5 were infected with 1x103 T. evansi and given sernai leaves extract with the dose of 30 mg/kg bw, 45 mg/kg bw, and 60 mg/kg bw respectively. The sernai extract was administered orally using a stomach tube for 3 consecutive days. The next day after last treatment, all rats were sacrificed and necropsied. Kidneys were collected for histopathological examination such as degeneration and necrosis of tubules cells, convoluted contortus duct lumen, and adhesion and atrophy of glomerulus. Sernai leaves extract with the dose of 45 mg/kg bw was the most effective dose to prevent kidney damage due to T. evansi infection.
Co-Authors . Nurliana Abdullah A. Muhammadar Aisyah Fadillah Tunnisa Aiza Annisa Alfin Oktarian Amalia Sutriana Amiruddin Amiruddin Arman Sayuti Asmawati Asmawati Awaluddin Awaluddin Awaluddin Awaluddin Bagus Dwijayanti Batubara, Agung Setia Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan chiara giri puspa Cut Nila Thasmi Darmawi Darmawi Dasrul Dasrul Dedi Fazriansyah Putra Denny Irmawati Denny Irmawati Hasan Dian Masyitha Dian Pratiwi Erdiansyah Rahmi Erdiansyah Rahmi Etriwati E Fadli A. Gani Firdus Firdus Fitriani Fitriani Gholib Gholib Hafizuddin Hafizuddin Hafizuddin Hafizuddin Hamdan Hamdan Hamdani Budiman Hamdani Budiman Hamny Sofyan Hattanul Mulia Indra Sitorus Jesica Ramadhanita Juli Melia Ketut Adnyane Mudite Lingga Surya Maret Daulay M Nur Salim M. Nur Salim Mahdi Abrar Mira Ayu Lestari Hasibuan Muchlisin Zainal Abidin Muhammad Hanafiah Muhammad Jalaluddin Mulyadi Adam Muslim Akmal Muslim Akmal Nazaruddin N Nazaruddin Nazaruddin Nazarudin Nazarudin Nellita Meutia Nurhazimah Nurhazimah Nuzul Asmilia Rahmiwati R Rastina Rastina Razali Daud Roslizawaty Roslizawaty Rosmaidar Rosmaidar Samadi S Siti Aisyah Srihadi Agungpriyono Sudi Indriany Sugito Sugito Syafrizal S Syafruddin Syafruddin T Reza Ferasyi T. Armansyah T. Armansyah T. Zahrial Helmi Tengku Muhammad Teuku Armansyah Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Ummu Balqis Wahyuni, Sri Yudha Fahrimal Zainuddin Zainuddin Zuhrawati Zuhrawati