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All Journal Jurnal Ilmu Pertanian Indonesia Prosiding Seminar Biologi JURNAL RISET AKUNTANSI GOING CONCERN ACCOUNTABILITY Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Asy-Syari'ah Tazkia Islamic Finance and Business Review Risâlah, Jurnal Pendidikan dan Studi Islam Jurnal Penelitian Pendidikan IPA (JPPIPA) Inovasi: Jurnal Ilmiah Ilmu Manajemen Jurnal Bidang Ilmu Kesehatan Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) Dinasti International Journal of Education Management and Social Science Jurnal Untuk Masyarakat Sehat (JUKMAS) Abdi Laksana : Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian kepada Masyarakat Nusantara Journal of Industrial Engineering & Management Research (JIEMAR) JURNAL ABDIMAS TRIDHARMA MANAJEMEN INTERNATIONAL JOURNAL OF SOCIAL, POLICY AND LAW (IJOSPL) Jurnal Ilmiah Raflesia Akuntansi Proceeding Biology Education Conference Rengganis Jurnal Pengabdian Masyarakat Jurnal Pajak dan Bisnis (Journal of Tax and Business) Annals of Human Resource Management Research Balance : Jurnal Akuntansi dan Manajemen Arus Jurnal Psikologi dan Pendidikan Sewagati Intelektual : Jurnal Penelitian Dosen Akuntansi Bukhori: Kajian Ekonomi dan Keuangan Islam Jurnal Pengabdian Masyarakat Bangsa The Economic Review of Pesantren Jurnal Pelayanan dan Pengabdian Kesehatan untuk Masyarakat Pasundan Community Service Development Jurnal Sistem Informasi dan Manajemen Policy Brief Pertanian, Kelautan, dan Biosains Tropika Journal of Social Work and Science Education Jurnal Abdi Citra Jurnal Nusa Akuntansi Halal Studies and Society Qaulan Baligha: Jurnal Ilmu Manajemen Dakwah
Neneng Hasanah
Research Centre for Biotechnology, LIPI. Jl. Raya Bogor Km 46, Kec. Cibinong, Kab. Bogor, 16911. Indonesia. Telp: +62-21-8754587.
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Journal : Jurnal Ilmu Pertanian Indonesia

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Bioprospecting of Pectinase-Producing Bacteria from Marine Actinomycetes Hasanah, Neneng; Nadhifah, Hana; Rahayuningsih, Mulyorini; Atikana, Akhirta; Ratnakomala, Shanti; Lisdiyanti, Puspita; Rahmani, Nanik
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.689

Abstract

Pectinase is used in a variety of industries, including fruit juice production, textile processing, papermaking, biodegumming, coffee and tea manufacturing, medicines, feed, and nutraceuticals. The demand for pectinase enzymes grows year after year. Industrial applications require high-activity enzymes that can increase considerable product yields while also improving based on needs and byproduct use. Indonesia's industry continues to rely on imported pectinase enzymes. Indonesia, on the other hand, possesses megabiodiversity, particularly marine microorganisms, which have the potential to provide new enzymes with high activity for industrial applications. This work aims to undertake bioprospecting of marine actinomycetes producing pectinase enzymes that have the capacity to hydrolyze pectin polymer, both from commercial and biomass sources. A total of 20 marine actinomycetes isolates from sediment, seawater, and sponges were bioprospected, and one isolate was selected with high pectinase activity (BLH 1.20), which was then used to characterize pH, temperature, hydrolysis analysis on pectin polymers, and isolate identification using 16s rRNA analysis. The selected isolate (BLH 1.20) performed best in a sodium phosphate buffer with a pH of 6.0 and a temperature of 30°C, achieving an activity of 5.4 U/mL. The 16S rRNA analysis revealed that the isolate is from the genus Streptomyces and the species Streptomyces sampsonii. Keywords: bioprospecting, marine Actinomycetes, pectinase, Streptomyces sampsonii
Co-Authors Agus Afandi Agustin, Inka Nadiah Agustina, Santi Ahmad Fikri Ahmad Syukri Aisyah, Tasya Nur Akaputri, Della Erriani Anggini, Karlita Arif R, Kasmin Asmawati Asmawati Atikana, Akhirta Azijah, Izattul Azzahra, Amelia Desriani Desriani Dewi Hasanah Dharmarianti, Dian Putri Dwi Endah Kusumawati Dzikri, Mohd. Iqbal EDI SAPUTRA Edy Kusnadi Ekafitrianda, J. M. Eko Prabowo, Eko Endri Purnomo Fatwanti, Devi Fauzi, Ikbar Fitria Sari Fitriani, Eva Fitriyah Fitriyah Galantika, Adinda Evan Geulista Hamzah Haq, Daffa Aqomal Hardjanto Haryanti, Ely Hasan, Afrizal Hasanah, Qoriatul Hendra Hendra Herawati, Ita Ilham, Hadiid Ushama indriyani, jihan Putri Isnarayani, Yuni Jessica, Melia Lestari, Neneng Dini Indah Lina Herlina M Solehah, Siti M. Syahran Jailani Maisah Maisah MARIA BINTANG Mawarni, Endang Siti Miftahul Huda Miftahul Jannah Mohammad Iqbal Irfany Muhajir Muhajir, Muhajir Muhammadi, Acep Muwahid Mulyorini Rahayuningsih Mustika Mustika Muthohharoh, Marhamah Nadhifah, Hana NANIK RAHMANI Napisah Napisah, Napisah Nawang Sari, Dewi Neni, Neni Nofantoro, Dwi Nugroho, Sindunata Nurhaeni Nursaleha, Ervina Priyanto, Anindia Meil Purba, Elvrida Triana BR PUSPITA LISDIYANTI Putri, Dwi Angraeni Putri, Narti Eka Putri, Uly Anggraeni Rahmalia, Desi Ramadhan, Mustarim Ramadini, Kintan Nur Restiyani, Destika Riana Rizky, Riana Risthi, Alyssa Rosa, Tina Salahuddin Salahuddin Sanusi, Anisa Sarah Salsabila Sarfilianty Anggiani, Sarfilianty Sari, Dewi Nawang SHANTI RATNAKOMALA Shibghotillah, Nur Sihite, Debby Alfani Siradju, Diva Athaya Fairuz Siswani, Sri Siti Nurfadilah Siti Zubaidah Sofran, Sidorus Alfandrianus Su'ud, Su'ud SUDARMONO SUDARMONO Sugeng, Andry Sulistyowati, Yeny Suparman, Alviani Sutihat, Ihat Syamsul Falah Tamirano , Randi Velani, Intan Tunandha Widiyati, Dian Wiwit Amrinola Yatmi, Wanda Tri Yeni Nuraeni, Yeni Yulandari, Sri Zenabia, Tsarina Zulqarnin, Zulqarnin