Heri Setiyo Bekti
Department Of Medical Laboratory Technology, Politeknik Kesehatan Kemenkes Denpasar, Denpasar, Bali, Indonesia And Center Of Excelence In Science And Technology, Polytechnic Of Health Denpasar, Bali, Indonesia

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Analysis of LT and ST Genes of Enterotoxigenic Escherichia coli Using Polymerase Chain Reaction Method in Lawar Plek from Ketewel Village, Gianyar I Made Yogi Hardiyanta; I Gusti Agung Ayu Putu Swastini; Heri Setiyo Bekti
MEDICA (International Medical Scientific Journal) Vol. 6 No. 2 (2024): MEDICA (International Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i2.504

Abstract

Lawar plek is a traditional Balinese dish made from raw meat, which poses a potential risk of contamination by pathogenic bacteria, including Enterotoxigenic Escherichia coli (ETEC). This study aimed to identify the presence of ETEC in lawar plek samples from Ketewel Village, Gianyar. This research uses molecular methods. The identification was conducted in two stages: initial isolation of suspected bacteria on Eosin Methylene Blue Agar (EMBA), followed by molecular confirmation using Polymerase Chain Reaction (PCR). The study results show that in EMBA culture, 4 out of 12 samples (33.33%) showed colonies presumptively identified as E. coli. However, PCR analysis revealed that none of these isolates carried the LT (heat-labile toxin) or ST (heat-stable toxin) genes characteristic of ETEC. The conclusion shows that neither the LT (heat-labile toxin) nor the ST (heat-stable toxin) virulence genes of Enterotoxigenic Escherichia coli were found in any of the samples tested as suspected Escherichia coli colonies on EMBA.
Examination of Total Cholesterol Levels in the Elderly with Hypertension (Case Study at Abiansemal I Health Center, Badung Regency-Bali) Komang Ayu Adinda Paramitha Triyani; Heri Setiyo Bekti; I Gusti Agung Ayu Dharmawati
Laboratory Journal of Infectious Diseases Vol. 1 No. 1 (2025): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/ljid.v1i1.2246

Abstract

An elderly person is someone who is 60 years or older. As we age, changes in the structure and function of cells, tissues, and organ systems occur in the body, increasing the risk of hypertension. Hypertension is a multifactorial disease that can be caused by cholesterol. Hypercholesterolemia can clog peripheral blood vessels, thereby triggering hypertension. The purpose of this study was to describe total cholesterols levels among elderly individuals with hypertension at Abiansemal I Health Center, Badung Regency, Bali. This type of study used is descriptive research using non-probability techniques, purposive sampling. Total cholesterol levels were measured using the POCT method in 44 elderly participants with hypertension; 14 individuals had borderline cholesterol levels, and 17 individuals (38,6%) had high total cholesterol levels. Based on the characteristics of the respondent's dominant gender with High cholesterol levels in women were 10 people (22,7%). Based on Body mass index (BMI) is dominant among respondents with high total cholesterol levels in the normal BMI category there were 11 people (25,0%). It can be concluded that most elderly individuals with hypertension at Abiansemal I Community Health Center had high total cholesterol levels.
Co-Authors Ade Dharmawan Agus Sri Lestari Amany, Hana Amirah ANDI UDIN SARANSI Anniwati, Leonita Aprilia Rakhmawati Aprilia Rakhmawati Aprilia Rakhmawati Aprilia Rakhmawati Arjani, Ida Ayu Made Sri Artawa, I Made Budi Arwati, Ketut Lilik Ayu Sukma Wedha Kristyanti Burhannuddin Burhanuddin, Burhannuddin Cok Dewi Widhya Hana Sundari Cok Dewi Widhya HS Cok Dewi Widhya HS Cokorda Dewi Widhya Hana Sundari Daniel, Elvera Sukma Desak Gede Dwi Agustini Desak Made Lestari Dewi Dewa Ayu Niti Rahayu Putri Dewi, Kadek Indra Trisna Dewi, Ni Km Ayu Kusuma Dewi, Ni Nyoman Astika Dewi, Pande Putu Ayu Patria Dharmawaiswari, Anak Agung Ayu Ratih Dharmawati, I Gst Agung Ayu Dwija, Ida Bagus Nyoman Putra Farsida, Farsida Fusvita Merdekawati Ganjar Noviar Gusti Agung Ayu Dharmawati Haribaik, Anastaysia Annisa Hasfika, Essydhea Husna, Ismalia I Gede Sudarmanto I Gusti Agung Ayu Dharmawati I Gusti Agung Ayu Dharmawati I Gusti Agung Ayu Putu Swastini I Gusti Agung Dewi Sarihati I Gusti Agung Dewi Sarihati I Gusti Ayu Made Wulan Diantari I Gusti Ayu Sri Dhyanaputri I Gusti Ayu Sri Dhyanaputri I Gusti Ngurah Teja Pratama I Made Dwipa Cahyana I Made Krishna Paramartha I Made Suarjana I Made Suarjana I Made Subrata, I Made I Made Yogi Hardiyanta I Made Yogi Hardiyanta I Nyoman Gejir I Nyoman Jirna I Nyoman Purna I Wayan Karta I Wayan Karta Ida Ayu Krisna Dwipayanti Ida Ayu Sri Arjani Ida Bagus Oka Suyasa Ida Bagus Oka Suyasa Indriyani Indriyani Jannah Sofy Yanty Kadek Ika Surya Cahyani Komang Ayu Adinda Paramitha Triyani Kosasih, Audia Nizhma Nabila Kurniawan, Surya Bayu Luh Ade Wilan Krisna Luh Ade Wilankrisna Luh Mitha Ari Utami Luh Putu Laksmi Mahastuti, Shri Ayu Purnami Mardhia Mardhia Marpaung, Nurasi Lidya E. Mastra , Nyoman Merdekawati, Fusvita Mochamad Choirul Hadi Ni Kadek Wahyu Diana Sari Ni Komang Erny Astiti Ni Komang Juniawati Ni Komang Omik Trianita Udiana Ni Komang Sri Narayanthi Ni Nyoman Astika Dewi Ni Putu Ditya Prabandari Dewi Ni Putu Ditya Prabandari Dewi Ni Putu Ditya Prabandari Dewi Ni Putu Intan Maha Ayu Diyanti Ni Putu Lisa Anjani Ni Putu Yulia Rahma Dewi Ni Wayan Rika Kumara Dewi Noviar, Ganjar Nur Habibah Nyoman Mastra Posmaningsih, Dewa Ayu Agustini Pradnya Yasa, Ni Putu Candra Dewi Prianggandanni, Made Ayu Vita Purwani Ruth, Grace Monica Putu Amrita Paramahita Putu Diah Wahyuni Rakhmawati, Aprilia Rike Syahniar Rinawati, Luh Putu Rindi, Oktavelendi Dhaneta Graha Rofi'ah, Lisa Khofidatur Ruspawan, I Dewa Made Savitri, Ni Putu Ayu Dani Sawitri, Anak Agung Sagung Sofi Yanty, Jannah Sudiadnyana, I Wayan Surya Bayu Kurniawan Suwarriana, I Nyoman Astra Suyasa, Ida Bagus Oka Tedja, I Gusti Ayu Wiradari Wikantari, Ni Made Ayu Kerti Wilankrisna, Luh Ade Yanty, Jannah Sofy