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All Journal International Journal of Public Health Science (IJPHS) Jurnal Sains dan Teknologi Jurnal Kebidanan Medical Laboratory Technology Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Meditory : The Journal of Medical Laboratory Jurnal Analis Kesehatan Jurnal Kesehatan Jurnal Bidan Cerdas Poltekita : Jurnal Ilmu Kesehatan Medicra (Journal of Medical Laboratory Science/Technology) Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Poltekita: Jurnal Pengabdian Masyarakat Jurnal Pengabmas Masyarakat Sehat Muhammadiyah Medical Journal Ahmar Metakarya: Jurnal Pengabdian Masyarakat Jurnal Pengabmas Masyarakat Sehat JURNAL SKALA HUSADA: THE JOURNAL OF HEALTH Jurnal Pendidikan Kesehatan (e-Journal) (Medical Scientific Journal) MESINA Media Penelitian dan Pengembangan Kesehatan MEDICA (International Medical Scientific Journal) Jurnal Analis Kesehatan Kendari Jurnal Info Kesehatan
Heri Setiyo Bekti
Department Of Medical Laboratory Technology, Politeknik Kesehatan Kemenkes Denpasar, Denpasar, Bali, Indonesia And Center Of Excelence In Science And Technology, Polytechnic Of Health Denpasar, Bali, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Impact of Sample Delay on Leukocyte and Platelet Parameters in Dengue Fever Patients: A Hematological Analysis Utami, Luh Mitha Ari; Bekti, Heri Setiyo; Dhyanaputri, I Gusti Ayu Sri
MEDICA (International Medical Scientific Journal) Vol. 6 No. 2 (2024): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i2.502

Abstract

Background: The diagnosis of Dengue Fever (DF) involves physical examination, laboratory tests, and confirmation primarily through thrombocytopenia and leukocyte abnormalities. Laboratory procedures play a crucial role; however, delays in complete blood count (CBC) analysis may influence hematological parameters, potentially affecting diagnostic accuracy. Objective: To determine the effect of 2-hour and 4-hour delays in CBC testing on leukocyte and platelet counts in patients with Dengue Fever. Methods: This experimental study used a posttest-only control group design with purposive sampling. Blood samples from DF patients were divided into three groups: immediate testing, 2-hour delay, and 4-hour delay. Data were analyzed using the Shapiro-Wilk test followed by Multivariate Analysis of Variance (MANOVA). Results: The mean leukocyte and platelet counts in the immediate testing group were 4.86×10³/μL and 61.55×10³/μL, respectively. In the 2-hour delay group, leukocyte counts slightly decreased to 4.79×10³/μL and platelet counts increased to 65.77×10³/μL. In the 4-hour delay group, leukocyte counts increased to 4.85×10³/μL while platelet counts decreased to 64×10³/μL. MANOVA results (Sig. = 1.000) indicated no significant differences among the groups. Conclusion: Delays of 2 and 4 hours in CBC testing did not significantly affect leukocyte and platelet counts in Dengue Fever patients. These findings suggest that short-term delays in sample analysis may not compromise hematological results in DF diagnosis.
Analysis of LT and ST Genes of Enterotoxigenic Escherichia coli Using Polymerase Chain Reaction Method in Lawar Plek from Ketewel Village, Gianyar Hardiyanta, I Made Yogi; Swastini, I Gusti Agung Ayu Putu; Bekti, Heri Setiyo
MEDICA (International Medical Scientific Journal) Vol. 6 No. 2 (2024): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i2.504

Abstract

Lawar plek is a traditional Balinese dish made from raw meat, which poses a potential risk of contamination by pathogenic bacteria, including Enterotoxigenic Escherichia coli (ETEC). This study aimed to identify the presence of ETEC in lawar plek samples from Ketewel Village, Gianyar. This research uses molecular methods. The identification was conducted in two stages: initial isolation of suspected bacteria on Eosin Methylene Blue Agar (EMBA), followed by molecular confirmation using Polymerase Chain Reaction (PCR). The study results show that in EMBA culture, 4 out of 12 samples (33.33%) showed colonies presumptively identified as E. coli. However, PCR analysis revealed that none of these isolates carried the LT (heat-labile toxin) or ST (heat-stable toxin) genes characteristic of ETEC. The conclusion shows that neither the LT (heat-labile toxin) nor the ST (heat-stable toxin) virulence genes of Enterotoxigenic Escherichia coli were found in any of the samples tested as suspected Escherichia coli colonies on EMBA.
The Overview of Nutritional Status and Blood Glucose Levels of Pregnant Women at the Ubud Health Center, Bali Rinawati, Luh Putu; Sarihati, I Gusti Agung Dewi; Sundari, Cok Dewi Widhya Hana; Dhyanaputri, I Gusti Ayu Sri; Bekti, Heri Setiyo; Dwija, Ida Bagus Nyoman Putra
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 4 (2024): February
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i4.2045

Abstract

  The nutritional status of pregnant women can be determined by measuring the size of mid-upper arm circumference (MUAC) and body mass index (BMI). Women with excessive nutritional status have a high risk of various complications. This study aims to describe the nutritional status and blood glucose levels of pregnant women at the Ubud Health Center, Bali. This descriptive study was conducted on 27 pregnant women who had antenatal care at the Ubud Health Center. MUAC size was measured using a measuring tape, while BMI was measured by calculating body weight in kilograms divided by height in meters squared. Blood glucose levels were measured using the point of care testing (POCT) method. From 27 respondents, it was found that 23 respondents had normal MUAC, 3 respondents had less than normal, and 1 respondent was obese. Based on BMI, 14 respondents had normal BMI, 5 respondents were overweight, and 8 respondents were obese. For blood glucose levels, all respondents have normal levels. Respondents who have MUAC less than normal can be at risk of chronic energy deficiency, while respondents with the obese category can be at risk of GDM, hypertension, and cesarean section. Pregnant women with GDM can increase the risk of type 2 DM, cardiovascular disease and have the potential to give birth macrosomic babies. Pregnant women with GDM almost never give complaints, so screening is necessary. Screening can be done by measuring the nutritional status of pregnant women and blood glucose levels during antenatal care at health center.
The Impact of Visfatin Level Against Blood Pressure Among Pregnant Women Rinawati, Luh Putu; Yanty, Jannah Sofy; Rakhmawati, Aprilia; Bekti, Heri Setiyo
JURNAL INFO KESEHATAN Vol 21 No 1 (2023): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol21.Iss1.912

Abstract

Visfatin, a protein with a molecular weight of 52 kDa, has been demonstrated to increase in the bloodstream. Visfatin has been shown in several studies to be a potential marker of preeclampsia. The objective of this study was to describe visfatin levels in pregnant women in Gianyar, Bali. An observational design was employed in this study to describe visfatin levels in 41 pregnant women in Ubud, Gianyar regency, Bali. Visfatin levels were determined by examining EDTA blood samples using the Elisa method and reading them at 450 nm on a microplate reader. The average value of visfatin levels was 6.49 ng/ml, according to the results. The average visfatin level based on hypertension blood pressure respondents was 3.74 ng/ml. This result was lower than that of normal blood pressure respondents.  This study's decrease in visfatin levels may have a smaller effect on physiological insulin resistance. The average visfatin level in second trimester respondents was 7.9 ng/ml higher than 5.7 ng/ml in third trimester respondents. The increase in visfatin levels suggests that visfatin may play a role in the pathogenesis of preeclampsia. Visfatin has the potential to be used as a biomarker to detect preeclampsia earlier, allowing pregnant women with preeclampsia to receive appropriate treatment.
Persistence of Antibody Response Against SARS-CoV-2 After Vaccination Bekti, Heri Setiyo; Habibah, Nur; Dharmawati, I Gusti Agung Ayu; Merdekawati, Fusvita; Noviar, Ganjar
JURNAL INFO KESEHATAN Vol 21 No 4 (2023): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol21.Iss4.1066

Abstract

SARS-CoV-2 is the causative agent of the disease known as COVID-19. COVID-19 is spreading very fast around the world. One of the immune responses that play a role in against SARS-CoV-2 infection is the production of antibodies, which is 3 weeks after infection. Where within 3 weeks after infection, antibodies will be produced against RBD and the S1 and S2 domains in glycoprotein S and nucleocapsid protein N. The ability of an antibody to inhibit viral infection is determined by its level or titer. This study aims to determine the description of antibody levels against SARS-CoV-2 after vaccination. This type of research is descriptive research. Measurement of antibody levels for SRBD SARS-CoV-2 was carried out using the CLIA method using the MAGLUMI tool. Of the 30 respondents, 23 people had received the third vaccine. The results of this study showed that the average level of SRBD antibodies against SARS-CoV-2 in respondents with 2 doses of vaccine (1.063,786 BAU/mL) was higher than in respondents with 3 doses of vaccine (535.651 BAU/mL). Vaccine intervals of more than 6 months (908.338 BAU/mL) have higher antibody levels than respondents with vaccine intervals of 1-6 months (228.006 BAU/mL). The conclusion of this study is the highest antibody titers are produced >6 months after vaccination, antibody titers are still detectable after 12 months of vaccination, and for further research, it can be measured antibody levels against SARS-CoV-2 from people who have got vaccination for a duration of 2 years or more.
The Development of Spondias pinnata (L.f) Kurz. Leaf Extract Toothpaste: Formulation, Physical Properties Evaluation and its Antibacterial Efficacy Against Streptococcus mutans Bacteria Habibah, Nur; Bekti, Heri Setiyo; Dewi, Ni Nyoman Astika; Dharmawati, I Gusti Agung Ayu; Krisna, Luh Ade Wilan; Mahastuti, Shri Ayu Purnami
JURNAL INFO KESEHATAN Vol 22 No 4 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss4.1669

Abstract

Cemcem (Spondias pinnata (L.f) Kurz) is a native Indonesian plant that has long been used as a traditional medicine. The ethanol extract of S. pinnata contains alkaloids, carbohydrates, flavonoids, triterpenoids, steroids, tannins, resins, and saponins. It has proven antibacterial activity against gingivitis and periodontitis bacteria, such as Streptococcus mutans. This study aims to develop a toothpaste formula with the active ingredient of S. pinnata leaf extract with various concentrations of 5, 15, and 30%; evaluate its properties; and measure its antibacterial effectiveness against Streptococcus mutans. The research method used a quasi-experimental method with a post-test-only control group design. The S. pinnata leaves were extracted with 96% ethanol solvent using the maceration technique. The properties of the toothpaste were evaluated based on homogeneity, spreadability, foam height, viscosity, and pH. The liking test was conducted to assess color, taste, scent, and texture based on the perception of 30 panelists. Antibacterial activity was tested using the well diffusion method. The test results confirm that all preparation formulas are homogeneous, the foam height is in the range of 4.33 - 21.33 cm, the spreadability is in the range of 3.44 - 4.53 cm2, the viscosity of toothpaste is in the range of 20767-67600 cps, and the pH value is in the range of 4.82 - 7.59. The results showed that toothpaste containing 5% of S. pinnata leaf extract met the physical and pH requirements and was most favored by panelists. This formula also showed antibacterial activity against S. mutans with an average inhibition zone diameter of 7.72 mm ± 0.439. Based on the study's results, it can be concluded that toothpaste containing 5% S. pinnata leaf extract effectively inhibits the growth of S. mutans bacteria by 54.33% compared to the positive control. Further research is suggested to optimize the concentration of S. pinnata leaf extract to maximize its antibacterial activity, without affecting its physical quality and organoleptic acceptance.
GAMBARAN KADAR HEMATOKRIT PADA PASIEN DEMAM BERDARAH DENGUE DI RUMAH SAKIT DAERAH MANGUSADA, BADUNG, BALI Sarihati, I Gusti Agung Dewi; Purwani Ruth, Grace Monica; Bekti, Heri Setiyo; Rinawati, Luh Putu
Jurnal Analis Kesehatan Kendari Vol. 6 No. 2 (2024): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 6 (2) Juni 2024)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v6i2.297

Abstract

ABSTRACT Dengue Dengue Hemorrhagic Fever (DHF) often causes plasma leakage which results in hemoconcentration which is characterized by increased hematocrit levels. The aim of this research is to determine the description of hematocrit levels in dengue fever patients using descriptive research methods. Data was taken by examining the hematocrit levels of inpatients in the Mangusada Regional Hospital laboratory. The sampling technique used was a saturated sampling technique and a sample size of 52 patients was obtained. The results showed that dengue fever patients had low hematocrit levels of 13.5%, normal 82.7% and high 3.8%. From these data it can be concluded that the majority of dengue fever patients have normal hematocrit levels. Key words: hematocrit, Dengue hemorrhagic fever ABSTRAK Demam Berdarah Dengue (DBD) sering menimbulkan kebocoran plasma yang mengakibatkan terjadinya hemokonsentrasi yang ditandai dengan meningkatnya kadar hematokrit. Tujuan dari penelitian ini adalah untuk mengetahui gambaran kadar hematokrit pada pasien DBD dengan menggunakan metode penelitian deskriptif. Data diambil dengan cara pemeriksaan kadar hematokrit pasien rawat inap di laboratorium Rumah Sakit Daerah Mangusada. Teknik sampling yang digunakan adalah teknik sampling jenuh dan didapatkan jumlah sampel sebanyak 52 pasien. Hasil penelitian menunjukkan bahwa pasien DBD yang memiliki kadar hematokrit rendah 13,5%, normal 82,7% dan tinggi 3,8 %. Dari data tersebut dapat disimpulkan bahwa mayoritas pasien DBD memiliki kadar hematokrit normal. Kata kunci: hematokrit, demam berdarah dengue
Edukasi PHBS Dengan Media Edukomik Dalam Upaya Meningkatkan Budaya Hidup Sehat Pada Anak Asuh Rumah BCC (Bali Caring Comunity) Desa Besakih, Kabupaten Karangasem Burhannuddin, Burhannuddin; Karta, I Wayan; Bekti, Heri Setiyo
Jurnal Pengabmas Masyarakat Sehat Vol 5, No 2 (2023): APRIL
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jpms.v5i2.2538

Abstract

Pembentukan budaya PHBS pada anak-anak dapat melalui tatanan rumah tangga, sekolah, dan organisasi sosial kemasyarakat yang berkecimpung dalam peningkatan sumber daya manusia. Salah satu organisasi yang telah terlibat aktif dalam pengembangan dan pemberdayaan anak-anak adalah Rumah Bali Caring Community (BCC). Permasalahan prioritas yang dihadapi anak asuh di Rumah BCC adalah kurangnya budaya PHBS. Dalam kegiatan pengabdian pada masyarakat ini diberikan solusi prioritas diantaranya melakukan kegiatan pemberdayaan kepada anak asuh BCC melalui pelatihan PHBS menggunakan media edukomik PHBS.Hasil kegiatan menunjukkan anak-anak asuh BCC selama kegiatan dapat menerima dengan baik materi dan metode penyuluhan yang digunakan. Anak-anak asuh BCC mengikuti seluruh rangkaian kegiatan dan berpartisipasi dengan aktif. Tingkat pengetahuan anak-anak asuh tentang budaya PHBS mengalami peningkatan. Pengetahuan anak asuh tentang PHBS dengan kategori baik meningkat dari 17 % sebelum kegiatan menjadi 83 % setelah kegiatan dilaksanakan. Semua anak asuh setelah kegiatan dilaksanakan telah mengenal tentang PHBS dan sebagian besar telah mampu mencontohkan dan mempraktekkan perilaku yang termasuk penerapan PHBS. Kegiatan pengabdian kepada masyarakat ini telah menghasilkan luaran berupa buku edukomik yang terdaftar HaKI, leaflet, drat artikel untuk publikasi dan peningkatan pengetahuan peserta 
Gambaran Kadar Hemoglobin pada Remaja Putri (Studi Kasus di SMA Negeri 2 Denpasar) Dewi, Kadek Indra Trisna; Bekti, Heri Setiyo; Krisna, Luh Ade Wilan; Dewi, Ni Nyoman Astika
Jurnal Skala Husada : The Journal of Health Vol 20, No 2 (2023): JSH: Jurnal Skala Husada-The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jsh:tjoh.v20i2.2758

Abstract

Prevalensi anemia pada remaja putri di dunia dilaporkan berkisar 40-88%. Remaja putri dengan anemia dapat mempengaruhi penurunan produktivitas kerja atau kemampuan akademik di sekolah karena kurangnya semangat belajar dan fokus. Anemia pada remaja putri dapat berlanjut selama kehidupan remaja putri tersebut dan berisiko menjadi ibu hamil dengan anemia yang dapat melahirkan bayi prematur dan berujung stunting. Penelitian ini bertujuan untuk mengetahui gambaran kadar hemoglobin pada remaja putri di SMA Negeri 2 Denpasar. Jenis penelitian yang digunakan adalah deskriptif dengan sampel sebanyak 42 orang remaja putri yang dipilih dengan teknik proportionate stratified random sampling. Pemeriksaan kadar hemoglobin dilakukan menggunakan metode Point of Care Testing (POCT) dengan menggunakan darah kapiler. Hasil penelitian menujukan bahwa kadar hemoglobin paling banyak normal yaitu sebanyak 29 responden (69,0%), kadar hemoglobin rendah sebanyak 7 responden (16,7%), dan kadar hemoglobin tinggi sebanyak 6 responden (14,3%). Gambaran kadar hemoglobin pada remaja putri di SMA Negeri 2 Denpasar sebagian besar memiliki kadar hemoglobin normal. 
Co-Authors Ade Dharmawan Agus Sri Lestari Amany, Hana Amirah ANDI UDIN SARANSI Anniwati, Leonita Aprilia Rakhmawati Aprilia Rakhmawati Aprilia Rakhmawati Aprilia Rakhmawati Arjani, Ida Ayu Made Sri Artawa, I Made Budi Arwati, Ketut Lilik Ayu Sukma Wedha Kristyanti Burhannuddin Burhanuddin, Burhannuddin Cahyana, I Made Dwipa Cok Dewi Widhya Hana Sundari Cok Dewi Widhya HS Cok Dewi Widhya HS Cokorda Dewi Widhya Hana Sundari Desak Gede Dwi Agustini Desak Made Lestari Dewi Dewa Ayu Niti Rahayu Putri Dewi, Kadek Indra Trisna Dewi, Ni Km Ayu Kusuma Dewi, Ni Nyoman Astika Dewi, Ni Putu Ditya Prabandari Dewi, Pande Putu Ayu Patria Dharmawaiswari, Anak Agung Ayu Ratih Dharmawati, I Gst Agung Ayu Dwija, Ida Bagus Nyoman Putra Farsida, Farsida Fusvita Merdekawati Ganjar Noviar Hardiyanta, I Made Yogi Haribaik, Anastaysia Annisa Hasfika, Essydhea Husna, Ismalia I Gusti Agung Ayu Dharmawati I Gusti Agung Ayu Dharmawati I Gusti Agung Dewi Sarihati I Gusti Agung Dewi Sarihati I Gusti Ayu Made Wulan Diantari I Gusti Ayu Sri Dhyanaputri I Gusti Ngurah Teja Pratama I Made Krishna Paramartha I Made Suarjana I Made Suarjana I Made Yogi Hardiyanta I Nyoman Jirna I Nyoman Purna I Wayan Karta I Wayan Karta Ida Ayu Krisna Dwipayanti Ida Ayu Sri Arjani Ida Bagus Oka Suyasa Ida Bagus Oka Suyasa Indriyani Indriyani Jannah Sofy Yanty Kadek Ika Surya Cahyani Kosasih, Audia Nizhma Nabila Kurniawan, Surya Bayu Luh Ade Wilan Krisna Luh Ade Wilankrisna Luh Putu Laksmi Mahastuti, Shri Ayu Purnami Mardhia Mardhia Marpaung, Nurasi Lidya E. Mastra , Nyoman Merdekawati, Fusvita Mochamad Choirul Hadi Ni Kadek Wahyu Diana Sari Ni Komang Erny Astiti Ni Komang Juniawati Ni Komang Omik Trianita Udiana Ni Komang Sri Narayanthi Ni Nyoman Astika Dewi Ni Putu Ditya Prabandari Dewi Ni Putu Ditya Prabandari Dewi Ni Putu Intan Maha Ayu Diyanti Ni Putu Lisa Anjani Ni Putu Yulia Rahma Dewi Ni Wayan Rika Kumara Dewi Noviar, Ganjar Nur Habibah Nur Habibah Nyoman Mastra Posmaningsih, Dewa Ayu Agustini Pradnya Yasa, Ni Putu Candra Dewi Prianggandanni, Made Ayu Vita Purwani Ruth, Grace Monica Putu Amrita Paramahita Putu Diah Wahyuni Rakhmawati, Aprilia Rike Syahniar Rinawati, Luh Putu Rindi, Oktavelendi Dhaneta Graha Rofi'ah, Lisa Khofidatur Ruspawan, I Dewa Made Savitri, Ni Putu Ayu Dani Sofi Yanty, Jannah Sudarmanto, I Gede Sudiadnyana, I Wayan Surya Bayu Kurniawan Suwarriana, I Nyoman Astra Suyasa, Ida Bagus Oka Swastini, I Gusti Agung Ayu Putu Tedja, I Gusti Ayu Wiradari Utami, Luh Mitha Ari Wikantari, Ni Made Ayu Kerti Wilankrisna, Luh Ade Yanty, Jannah Sofy