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All Journal Pastura; Journal Of Tropical Forage Science Jurnal Ilmu Ternak Jurnal Ilmu Ternak Veteriner Jurnal Ternak Tropika Jurnal Ilmiah Fillia Cendekia Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan ZIRAA'AH MAJALAH ILMIAH PERTANIAN Media Kontak Tani Ternak JANHUS: Jurnal Ilmu Peternakan Journal of Animal Husbandry Science Jurnal Teknologi Hasil Peternakan PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat Jurnal Produksi Ternak Terapan (JPTT) Farmers: Journal of Community Services Jurnal Agripet Animal Production Jurnal Veteriner
Nena Hilmia
Fakultas Peternakan Universitas Padjadjaran
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THE EFFECT SNPs R25C AND R25H LEPTIN GENE TO CHOLESTEROL AND TRIGLYCERIDE ON BLOOD OF ONGOLE CROSSBREED CATTLE Hilmia, Nena; Dudi, Dudi; Edianingsih, Primiani; Mushawir, Andi; Susilawati, Iin; Pangestu, Muhammad Syahid Panji; Widyastuti, Rini
ZIRAA'AH MAJALAH ILMIAH PERTANIAN Vol 50, No 1 (2025)
Publisher : Pusat Publikasi Jurnal Universitas Islam Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/zmip.v50i1.18069

Abstract

Single Nucleotide Polymorphisms (SNPs) on the Leptin gene may alter the quality and quantity of Leptin hormone, which plays an active role in energy metabolism and it can contribute to fat deposition in mammals. This study proposed to determine the effect of SNPs R25C and R25H on Cholesterol, and Triglycerides levels in the blood of Ongole Crossbreed cattle. The research was carried out experimentally with a Completely Randomized Design using the sixteen heads of Ongole Crossbreed. There were three different genotypes based on SNPs R25C and R25H: CC, TT, and  AA. The cattle were reared intensively for two months. The feed consists of corn kernels and concentrates, and It is calculated based on the Dry Matter (DM) intake, which is 3% of body weight. The result showed SNPs R25C and R25H on the Leptin gene did not have a significant effect on blood Cholesterol, and Triglyceride levels of Ongole Crossbreed cattle. Those SNPs in the Leptin gene cannot be used as selection markers in Ongole Crossbreed cattle. Further research is needed to include SNPs in other genes contributing to Cholesterol and Triglycerides on blood cattle
THE EFFECT SNPs R25C AND R25H LEPTIN GENE TO CHOLESTEROL AND TRIGLYCERIDE ON BLOOD OF ONGOLE CROSSBREED CATTLE Hilmia, Nena; Dudi, Dudi; Edianingsih, Primiani; Mushawir, Andi; Susilawati, Iin; Pangestu, Muhammad Syahid Panji; Widyastuti, Rini
ZIRAA'AH MAJALAH ILMIAH PERTANIAN Vol 50, No 1 (2025)
Publisher : Pusat Publikasi Jurnal Universitas Islam Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/zmip.v50i1.18069

Abstract

Single Nucleotide Polymorphisms (SNPs) on the Leptin gene may alter the quality and quantity of Leptin hormone, which plays an active role in energy metabolism and it can contribute to fat deposition in mammals. This study proposed to determine the effect of SNPs R25C and R25H on Cholesterol, and Triglycerides levels in the blood of Ongole Crossbreed cattle. The research was carried out experimentally with a Completely Randomized Design using the sixteen heads of Ongole Crossbreed. There were three different genotypes based on SNPs R25C and R25H: CC, TT, and  AA. The cattle were reared intensively for two months. The feed consists of corn kernels and concentrates, and It is calculated based on the Dry Matter (DM) intake, which is 3% of body weight. The result showed SNPs R25C and R25H on the Leptin gene did not have a significant effect on blood Cholesterol, and Triglyceride levels of Ongole Crossbreed cattle. Those SNPs in the Leptin gene cannot be used as selection markers in Ongole Crossbreed cattle. Further research is needed to include SNPs in other genes contributing to Cholesterol and Triglycerides on blood cattle
Sosialisasi Optimalisasi Produktivitas Domba di Kelompok Peternak Lentera Kirei, Kecamatan Cijulang Kabupaten Pangandaran Nurmeidiansyah, An An; Hilmia, Nena; Ismiraj, Muhammad Rifqi; Widyastuti, Rini
Farmers: Journal of Community Services Vol 6, No 1 (2025): Farmers: Journal of Community Services
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/fjcs.v6i1.61258

Abstract

Kesehatan ternak merupakan salah satu kunci penentu keberhasilan suatu usaha peternakan. Melalui penerapan manajemen kesehatan ternak yang baik dan dilakukan secara berkelanjutan, diharapkan dapat meningkatkan produktivitas ternak. Guna mencapai hal tersebut, diperlukan penyampaian informasi kepada peternak, khususnya bagi peternak yang masih menerapkan pola pemeliharaan tradisional. Salah satu metode penyampaian informasi yang sederhana dan dinilai efektif adalah melalui sosialisasi. Kegiatan Pengabdian Kepada Masyarakat (PKM) inidilaksanakandiKelompok Peternak Domba Lentera Kirei, Kecamatan Cijulang, Kabupaten Pangandaran. Kegiatan dilakukan dengan melakukan kunjungan secara langsung ke kandang peternak, dan selanjutnya dilakukan sosialisasi dan diskusi mengenai kesehatan hewan, serta observasi dan pemeriksaan fisik terhadap domba. Berdasarkan hasil pengamatan di lapangan terungkap bahwa, sebelum dilakukan sosialisasi ternyata mayoritas peternak telah memiliki pengetahuan dan kesadaran yang baik mengenai pentingnya kondisi kesehatan ternak, selain itu peternak juga telah memiliki pemahaman yang baik mengenai bagaimana cara memberikan pertolongan pertama pada domba yang sakit serta kondisi gawat darurat. Sebaliknya, pengetahuan tentang prinsip dasar pencegahan penyakit, pengetahuan tentang jenis penyakit pada domba masih sangat rendah, yaitu antara 30-40 %. Setelah dilakukan sosialisasi, terjadi peningkatan pengetahuan peternak terhadap semua parameter, terutama pengetahuan peternak terhadap prinsip dasar pencegahan penyakit, pengetahuan tentang jenis penyakit menular, penyakit tidak menular, dan penyakit reproduksi pada domba.
Identifikasi Keragaman Gen Serol Regulatory Element-Binding Protein 1 (SREBP-1) sebagai Kandidat Gen untuk Kualitas Daging Pada Sapi Pasundan Fauziah, Siti Ghina; Hilmia, Nena; Mayasari, Novi
Jurnal Teknologi Hasil Peternakan Vol 6, No 1 (2025): Maret
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jthp.v6i1.61281

Abstract

Sapi Pasundan merupakan sapi lokal yang memiliki potensi dalam memenuhi kebutuhan daging nasional khususnya di Jawa Barat. Kualitas daging salah satunya dipengaruhi oleh komposisi asam lemaknya. Sintesis asam lemak pada daging sapi dipengaruhi oleh protein yang diproduksi oleh gen SREBP-1 . Penelitian ini bertujuan untuk mengidentifikasi gen SREBP-1 sebagai kandidat gen untuk kualitas daging pada Sapi Pasundan. Metode penelitian yang digunakan adalah eksperimental eksploratif. Penelitian ini dilakukan dengan mengamplifikasi DNA dari 25 ekor Sapi Pasundan. Amplifikasi target DNA untuk mendeteksi polimorfisme 84-bp indel pada intron 5 gen SREBP-1 , dilakukan dengan teknik PCR, dengan primer spesifik. Visualisasi hasil PCR dilakukan dengan elektroforesis gel agarose. Sekuensing DNA target hasil PCR dilakukan untuk mengkonfirmasi mutasi pada gen SREBP-1 . Hasil penelitian menunjukkan adanya dua genotip, yaitu LL (96%) dan LS (4%), dengan frekuensi alel L sebesar 0,98 dan alel S sebesar 0,02. Selain itu, ditemukan dua mutasi baru, yaitu SNP g.9969T>C dan SNP g.10265A>G. Penelitian ini menunjukkan bahwa gen SREBP-1 pada Sapi Pasundan bersifat polimorfik dan berpotensi menjadi kandidat gen untuk kualitas daging yang berkaitan dengan komposisi asam lemak.
Reproductive Performance of Ongole Grade, Simmental, and Limousin Cattle Given Superovulation Treatment Pangestu, Muhamad Syahid Panji; Darodjah, Siti; Hilmia, Nena; Imron, Muhamad
Jurnal Agripet Vol 24, No 2 (2024): Volume 24, No. 2, October 2024
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v24i2.32335

Abstract

Reproductive technologies have the potential to enhance livestock productivity, with superovulation being one such method aimed at increasing the number of ovulated oocytes per estrous cycle through hormonal stimulation. This study aimed to evaluate the reproductive performance of three cattle breedsOngole Grade (OG) (n=20), Simmental (n=18), and Limousin (n=20)subjected to superovulation treatment. The cattle, aged 2.5 to 4 years with a body condition score of 3.5 to 4, were intensively managed. Superovulation was induced using CIDR (Controlled Internal Drug Release) for synchronization, followed by follicle-stimulating hormone (FSH) administration. Reproductive performance data, including age at first estrus, number of corpora lutea (CL), and embryo production, were collected over three estrous cycles. A quasi-experimental design was employed with breed as the primary treatment factor, and data were analyzed using analysis of variance (ANOVA). The results indicated that breed had a significant effect (P0.05) on the age of first estrus, with OG cattle reaching first estrus at 621.393.3 days, Simmental at 522.475.5 days, and Limousin at 570.488.1 days. However, no significant differences (P0.05) were observed among the breeds in terms of the number of CL, collected embryos, or transferable embryos across the superovulation cycles.
Identifikasi Keragaman Gen FASN (Fatty Acid Synthase) sebagai Kandidat Gen Kualitas Daging pada Sapi Pasundan Betina Putri, Verlia Dwi; Mayasari, Novi; Hernaman, Iman; Ramdani, Diky; Sulastri, Erni; Hilmia, Nena
Jurnal Teknologi Hasil Peternakan Vol 6, No 2 (2025): September
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jthp.v6i2.62816

Abstract

Pasundan cattle are one of Indonesia’s local cattle thathave the potential to be developed, especially in terms of meatquality. Meat quality is strongly influenced by fatty acidcomposition, which is closely related to gene regulation. This studyaims to identify the diversity of the FASN (fatty acid synthase) geneas a candidate gene for meat quality in female Pasundan cattle. Thisstudy used an experimental descriptive method with 25 DNAsamples of female Pasundan cattle with an age range of 1,5-2 years.DNA amplification using PCR was perfomed with specific primersdesigned with NCBI Primer Blast, flanking the target gene along560 bp. The analysis was followed by PCR-RFLP (RestrictionFragment Length Polymorphism) method using MscI enzyme todetect single nucleotide polymorphism (SNP) g.17924 A>G in exon39 and confirm the target mutation using direct sequencingmethod. The results showed only one genotype, the GG genotype(100%), with a G allele frequency of 1.00. Based on these results, itcan be concluded that the FASN gene mutation g.17924 A>Gmutation in Pasundan cattle is monomorphic. SNP g.17924 A>G inthe FASN gene has the potential to affect the fatty acid compositionof Pasundan female beef
Identifikasi Keragaman Gen Leptin Sebagai Kandidat Gen Kualitas Daging Pada Sapi Pasundan Betina Nurkamilah, Ghina; Hilmia, Nena; Putranto, Wendry Setiyadi
Jurnal Teknologi Hasil Peternakan Vol 6, No 2 (2025): September
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jthp.v6i2.62856

Abstract

Meat quality is influenced by genetic factors,environmental conditions, and their interactions. Variations in thenucleotide sequence of the leptin gene may affect the quality andquantity of leptin hormone, thereby influencing meat quality. Thisstudy aimed to identify the genetic diversity of the leptin genebased on nucleotide sequence mutations in Pasundan cattle raisedat the Ciparanje Beef Cattle Teaching Farm, Faculty of AnimalScience, Universitas Padjadjaran. This Study used 16 DNA samplesfrom female Pasundan cattle were isolated from blood andanalyzed. PCR amplification was performed to amplify a 620 bpfragment of the leptin gene using specific primers designed via theNCBI Primer-BLAST program, with an annealing temperature of59°C. PCR products were evaluated through electrophoresis, andsequencing was carried out to determine the nucleotide sequence.Sequence analysis and genotyping were conducted using BIOEDITand MEGA 11 software. The results revealed a Single NucleotidePolymorphism (SNP) at position g.1047C>T/R25C in the leptingene, with the CT genotype (56.35%) being more frequent than CC(18.8%) and TT (25%). Additionally, several point mutations inintron 1 were identified, including g.806G>A, g.845C>T,g.856G>A, g.868G>C, g.885C>T, g.911C>T, and g.913A>G,consisting of both transition and transversion mutations. Thesefindings indicate that the leptin gene in Pasundan cattle ispolymorphic. Leptin gene polymorphisms in Pasundan cattle arepotentially associated with meat quality traitrs and fat depositionvariation, thus providing a genetic basics for selection to improvethe quality of local cattle
Vaginal Cytological Changes and Estrus Response Following Estrus Synchronization Using a Double Prostaglandin-F₂α and Gonadotropin Releasing Hormone in Pasundan Heifers Rini Widyastuti; Azis, Azhar Abdul; Setiawan, Rangga; Hilmia, Nena; Lubis, Alkaustariyah; Ridlo, Muhammad Rosyid
Jurnal Veteriner Vol. 27 No. 1 (2026)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Pasundan cattle, an indigenous breed native to West Java, Indonesia, demonstrate significant adaptability and reproductive potential. However, their declining populations pose a threat to genetic conservation. Effective estrus synchronization is essential for enhancing reproductive efficiency and supporting fixed-time artificial insemination (FTAI) programs. Vaginal cytology profiling offers a reliable, noninvasive method for assessing estrus by evaluating the dynamics of epithelial and leukocyte cells, which reflect hormonal fluctuations. This study was aimed to investigate the cytological dynamics of vaginal epithelial, leukocyte cells and estrus response in Pasundan heifers subjected to double injection of prostaglandin F₂α (PGF₂α) and gonadotropin-releasing hormone (GnRH) synchronization. Nine Pasundan heifers, aged 1.5–2.0 years, were treated with PGF₂α with 2.75 μg/kg BW on days 0 and 11, followed by 2 μg/kg BW GnRH on day 12. Vaginal smears were collected daily from days 0 to 15, stained with 5% Giemsa, and examined microscopically to determine the proportions of parabasal, intermediate, superficial, keratinized, and leukocyte cells. The results indicated that the initial cytology showed a luteal phase dominated by parabasal (31.2%) and intermediate (30.6%) cells. Following hormonal treatment, superficial cells peaked at 70.9% on day 13, indicating estrus and estrogen dominance. By days 14–15, keratinized and parabasal cells had increased, confirming post-ovulatory luteal activity. The protocol achieved complete (100%) estrus synchronization among all heifers. It can be concluded that the double PGF₂ α-GnRH protocol effectively synchronized estrus in Pasundan heifers. Vaginal cytology has proven to be a sensitive and practical tool for monitoring reproductive phases, thereby supporting breeding management and conservation of this native genetic resource.
Identifikasi Keragaman Gen Thyroglobulin (TG) sebagai Kandidat Gen Kualitas Daging pada Sapi Pasundan Betina Raihan, Alfina; hilmia, Nena; Putranto, Wendry Setiyadi; Hidayat, Rahmat; Hadi, Didik Nurul
Jurnal Teknologi Hasil Peternakan Vol 7, No 1 (2026): Maret 2026
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jthp.v7i1.63634

Abstract

Sapi Pasundan merupakan sumber penyedia daging dengan kualitas baik yang potensial di Jawa Barat. Kualitas daging salah satunya ditentukan oleh komposisi asam lemak yang memengaruhi cita rasa, keempukan, juiceness dan nilai gizinya. Komposisi asam lemak dipengaruhi oleh faktor genetik dan lingkungan, salah satunya adalah gen thyroglobulin. Penelitian ini bertujuan untuk mengidentifikasi keragaman gen thyroglobulin sebagai kandidat gen kualitas daging pada Sapi Pasundan Betina. Metode penelitian yang digunakan adalah deskriptif eksploratif. Sampel DNA berasal dari 20 ekor Sapi Pasundan Betina yang dipelihara di Kandang Sapi Potong Ciparanje, Fakultas Peternakan, UNPAD. Metode yang digunakan adalah PCR (Polymerase Chain Reaction) dan sequencing. DNA target diamplifikasi melalui teknik PCR dengan primer spesifik yang di desain menggunakan program Primer Blast NCBI sepanjang 674 bp. Hasil PCR disekuensing untuk melihat runutan nukleotidanya. Analisis mutasi dilakukan dengan pensejajaran hasil sekuensing dengan data gen thyroglobulin di NCBI menggunakan program Bioedit dan Mega 11. Hasil penelitian menunjukkan terdapat 10 mutasi dengan tiga mutasi yang ditemukan merupakan mutasi baru yaitu g.66G>A, g.81T>C, dan g.416G>A dan tujuh mutasi lainnya yaitu g.240G>T, g.257C>T, g.274C>A, g.335A>G, g.387G>A, g.537C>T, dan g.552T>C. Penelitian ini menunjukkan bahwa gen thyroglobulin pada Sapi Pasundan Betina bersifat polimorfik. Kata Kunci: Thyroglobulin, Sapi Pasundan, Mutasi, Kualitas Daging, Asam Lemak
Co-Authors Aine Nur Firdausya Alfiah Almas Hamid An An Nurmeidiansyah, An An Ani Rosmayanti Annisaa Yusrina Asmara, Indrawati Yudha Asri Wulansari, Asri Azis, Akbar Azis, Azhar Abdul Bayani, Matni Syifa C Sumantri C. Sumantri Cece Sumantri Dani Garnida Dani Garnida Darlian Fachruddin Daud, Andre Rivianda Dedi Rahmat Dedi Rahmat Dedi Rahmat Dedi Rahmat Dedi Rahmat Didik N Hadi Didik Nurul Hadi Dikdik Nurulhadi Diky Ramdani Dinah Sholikhah Dity Asa Priyastomo Dudi Dudi dudi, dudi Edianingsih, Primiani Estria Furry Pramudyaswari Fauziah, Siti Ghina Gurnadi E Hadi, Didik Nurul HAIFA FARRAS IZDIHAR Harry Pariswara Heni Indrijani Husna, Afifah Iin Susilawati Iman Hernaman Imron, Muhamad Indra Firmansyah Indrawati Yudha Asmara Irma Irma Ismiraj, Muhammad Rifqi Johar Arifin Laelatul Choiriyah Lubis, Alkaustariyah M. Fatah Wiyatna Maulana, Muhammad Irfan Mokhamad Fakhrul Ulum, Mokhamad Muhammad Syahid Panji Pangestu Mushawir, Andi Nurcholidah Solihati Nurkamilah, Ghina Oloan Parlindungan Pangestu, Muhamad Syahid Panji Pangestu, Muhammad Syahid Panji Primiani Edianingsih Primiani Edianingsih Putri, Verlia Dwi R Priyanto R. Priyanto R.R Noor Raden Febrianto Christi Rahmat Hidayat Raihan, Alfina Rangga Setiawan Ridlo, Muhammad Rosyid Riki Akbar Rosidin Rini Widyastuti Roesillah, Diar Romi Zamhir Islami Ronny Rahman Noor Rudi Priyanto Savitri Novelina Septaria Jodiansyah Sikin Sikin Siti Darodjah Siti Darodjah Rasad Siti Darodjah Rasad Siti Darodjah, Siti Sri Wahyuni Siswanti Sulastri, Erni Supraptono Supraptono Syifa Khairunnisa Toha, Toha Wendry Setiyadi Putranto Weni Kurniati Winangun, Kikin Yurleni Yurleni