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Journal : Journal of Innovative and Creativity

Quality Of Frozen Cement From Landrace Boars In Modified Citrate Diluents With Different Glycerol Concentrations Yohanes Paulus Martinus Bay; Thomas Mata Hine; Yustiany Yuliani Bette; W. Marlene Nalley
Journal of Innovative and Creativity Vol. 5 No. 3 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i3.4269

Abstract

The purpose of this study was to determine the quality of frozen semen from landrace boars in modified citrate diluents with different glycerol concentrations. The research material used was fresh cement from 2-3 year old landrace boar studs from 4 boars. This study used an experimental study with a completely randomized design method consisting of 4 treatments and 4 replications. The treatments in question are T1: S-KT + G 3%, T2: S-KT + G 5%, T3: S-KT + G 7% and T4: S-KT + G 9%. The diluted cement was stored at room temperature (27-28℃) for two hours (holding time), then centrifuged at 3000 RPM for 15 minutes. After centrifugation, the supernatant was discarded and the centrifuged pellet was re-diluted with basic diluent. The diluted semen was packed in 0.5 Ml straws, then packed, arranged in a freezing rack, and then equilibrated at 3-5℃ for 2 hours. Then the straws were frozen above the surface of liquid N2 with a distance of 10 cm at a temperature of ±-110℃ for 10 minutes. The results of this study indicate that the addition of different glycerols did not have a significant effect (P>0.05) on the motility, viability, abnormality and recovery rate of spermatozoa. It was concluded that the addition of different glycerols had the same impact but were not suitable for IB.
Co-Authors A. Kaka A. Parakkasi Abdullah Baharun Alfred Nubatonis Aloysius Marawali Aloysius Marawali Aloysius Marawali Aloysius Marawali Arief Boediono Ayang C. Tafuli Banamtuan, A. N. Bayu Rosadi Bette, Yustiany Y Bire Lawa, Alvrado Burhanuddin * Burhanuddin . D. J. Djawapatty Doda Dengi, Donatus Donatus Doda Dengi Dondin Sajuthi Dui, Theresia A. A. T. Enny T Setiatin Franky M S Telupere Fransiska Onya Pandahuki George Valentino Kosat Grasele, Fransiskus Allianus Gue, Febronia Dhenge Gunawan G Henderiana Belli Henderiana L.L Belli Heri Armadianto Heri Armadianto Hilly, Fanny Tesalonika Honin, Olfi Mariana Iman Supriatna Ina Mato, Sefania J A Tamoes Jernih Blegur Johanis Ly Kihe, Johny Nada Kii, Mariana B.I. kirenius uly Kirenius Uly Kirenius Uly Kirenius Uly Kirenius Uly Kristina Meldiana Hayati L. L Belli, Henderiana L. L Belli, Henderiana Lawa, Alvrado B Mardan Laes Papituan Mardemus Misa Maria E.E. Fernandez Maria Epifania Barek Maria Orsella Rindiani Mariana Nenobais Maritje Aleonor Hilakore Masae, Sinoptis Melkiur Nalley, W. Marlene Nalley, Welmince M. Nalley, Wilmientje M Nalley, Wilmientje M. Ni Made Paramita Setyani Nuba, Maria Deran Odo, Remigius Abha Petrus Kune Petrus Kune Petrus Kune Petrus Kune Petrus Kune Riwu, Agustinus R. Sefania Ina Mato Selsiana Dongkot Setyani, Ni M P Siena, Sofia M.S. Sofia Marlize Soleman Manaze Weiri Sinda Stef Amiron Ratu Tapatab, Kelvin Alberto Imanuel Telupere, Franky M S Toto, Novaldy Imanuel Tree May Thiessen Outang Twen O. Dami Dato Ulu, Krista Florida Uly, Kirenius W. M. Nalley W. Marlene Nalley W. Marlene Nalley W. Marlene Nalley Wilmientje M. Nalley Wilmientje Marlen Nalley Wilmientje Marlene Mesang Nalley Wilmientje Marlene Nalley Wilmientje Marlene Nalley, Wilmientje Marlene Wilmintje Marlene Nalley Wirona Adu Yohanes Paulus Martinus Bay Yohanis Umbu Daku Waluwanja Yustiani Yuliana Bette Yustiany Yuliani Bette Yusuf Sae