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Molecular Docking in red spinach plants (Amaranthus tricolor L.) as an inhibitory agent for genetic anemia Fattara, Fajar Putra; Idami, Zahratul; Silalahi, Ali Affan
Science Midwifery Vol 12 No 3 (2024): August: Health Sciences and related fields
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v12i3.1660

Abstract

This study aims to evaluate the potential of active compounds from the Red Spinach plant (Amaranthus tricolor L.) as an inhibitor agent for the genetic disease anemia through a molecular docking approach. The method used involved analyzing the 3D structures of four active compounds Phenol, Lutein, Quercetin, and Zeaxanthin in these plants, which were downloaded from PubChem and processed using the Swiss software Target Prediction, Super Prediction, PyMol, and PyRx. The interaction between the compound and the biological target, in this case, Erythropoietin, is evaluated to determine binding energy, binding affinity, and stability. The results showed that Phenol and Quercetin had a significant interaction with the lowest binding energy of -4.8 kcal/mol and -8.1 kcal/mol respectively, as well as the lowest RMSD value (0 Å), indicating good interaction stability with the protein target. The 3D molecular structure of these two compounds also indicates the presence of hydrogen bonds and effective interactions with amino acids, as well as low toxicity and favorable affinity energies. Based on these results, Phenol and Quercetin were identified as potential candidates in the development of drugs to treat anemia genetic diseases, thanks to their favorable interaction properties and molecular structural characteristics. This research underlines the importance of the molecular docking approach in identifying bioactive compounds that can be further developed as therapies for the genetic disease anemia.
The Phonological Interference of Acehnese in Pronouncing Indonesian Language Suhery, Dedy; Idami, Zahratul; Wati, Shafrida
Journal of Languages and Language Teaching Vol. 12 No. 1 (2024): January
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jollt.v12i1.9554

Abstract

Linguistic issues such as interference and integration frequently arise in multilingual environment, which frequently result in barriers to second language production. This study examines at how language interaction between Acehnese and Indonesian has affected the Indonesian language in Langsa, with a particular emphasis on consonant variations and interference. The objective of this study was to investigate the influence of phonological interference on Acehnese speakers when speaking the Indonesian language, with a specific focus on observing variations in consonant sounds using auditory means.  The study conducted a detailed examination of common variances in Indonesian pronunciation among Acehnese speakers.  This qualitative research was carried out in Langsa, Aceh Province, involving  40 individuals who are native speakers of Acehnese. The participants consisted of 20 males and 20 females, encompassing a range of ages.  The collected data was evaluated using the PRAAT software.  The findings revealed that there was interference in pronunciation, particularly in the form of devoicing, which is the absence of vowel resonance. This was observed in the words "[ap. di]" and "[ʤa.wap]."  This interference leads to a significant level of devoicing, reaching 90% and 100% respectively.  Furthermore, the study revealed phonological interference in words such as "[thə.linga]", "[tə.thap]", and "[a.hat]", with speakers demonstrating devoicing rates of 40%, 40%, and 100% respectively.  The findings showed the complex nature of phonological interference in the pronunciation of the Indonesian language by Acehnese speakers.  These findings served as a foundation for the development of more effective strategies for acquiring a second language, particularly in diglossic environments like Indonesia.
Teknik RAPD (Random Applied Polymorphic DNA) untuk Analisis Keanekaragaman Genetik pada Tanaman Ciplukan (Physalis angulata L.) di Kabupaten Labuhan Batu Utara Yusvita, Diana; Idami, Zahratul
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12889

Abstract

The ciplukan plant (Physalisangulata L.) can be used as traditional medicine, but so far people only know that this plant can only be used for the fruit. This research aims to determine the results of RAPD primary analysis which can produce polymorphic bands in Ciplukan Plants (Physalisangulata L.) in North Labuhan Batu Regency using different primers. This research is a quantitative research. Sampling was carried out in Bondar Village and Simpang Tiga Village, Kualuh Leidong District, North Labuhan Batu Regency. Then continued with molecular analysis at the Genetics Laboratory, Faculty of Science and Technology, North Sumatra State Islamic University, Medan. The results of RAPD (Random Applied Polymorphism DNA) analysis of ciplukan plants in North Labuhan Batu district using six different primers, namely the length of the nucleotides in the amplified DNA fragments, are shown in primers OPA-2 & OPA-3 are 600bp-3000bp, while OPA-5, OPA-7, OPD -11, & OPD-13 are 500bp-3000bp with different nucleotide sequences. The differences in the length and number of nucleotide sequences in ciplukan plants are caused by the plants used coming from different locations and several factors such as the environment, population size, natural conditions, reproductive methods and natural selection.
Potensi Biomassa Dan Cadangan Karbon Pada Tegakan Hutan Sekunder Di Kawasan Resort 7 Sopotinjak Taman Nasional Batang Gadis Mandailing Natal Sumatera Utara Harahap, Atika Rahmah; Hutasuhut, Melfa Aisyah; Idami, Zahratul
BEST Journal (Biology Education, Sains and Technology) Vol 6, No 2 (2023): September 2023
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v6i2.8661

Abstract

Meningkatnya karbondioksida di atmosfer menyebabkan timbulnya masalah lingkungan. Dalam menurunkan emisi CO2 dapat dilakukan penyerapan oleh vegetasi hutan dan akan menyusun karbohidrat sebagai hasil fotosintesis yang disimpan dalam bentuk biomassa. Tujuan penelitian untuk mengetahui jumlah biomassa dan cadangan karbon tegakan. Metode yang digunakan adalah metode non destruktif sebanyak 32 plot dalam 4 transek. Hasil penelitian diperoleh biomassa tegakan hutan sekunder di Resort 7 Sopotinjak Taman Nasional Batang Gadis sebesar 13147,56 ton/ha dengan nilai rata- rata 4382,52 ton/ha sedangkan nilai cadangan karbonnya sebesar 6579,52 ton/ha dan nilai rata- rata 2193,173 ton/ha. Dari hasil tersebut menunjukkan bahwa nilai biomassa dan cadangan karbon tegakan hutan sekunder di Resort 7 Sopotinjak relatif baik dari fungsi ekologisnya sebagai penyimpan biomassa dan cadangan karbon karena semkain banyak komposisi vegetasi, kerapatan vegetasi, faktor fisik lingkungannya akan berpengaruh terhadap jumlah simpanan biomassa dan cadangan karbon yang terkandung dalam hutan tetrsebut
UJI MOLEKULER MENGGUNAKAN GEN rbcL PADA TUMBUHAN PEGAGAN (Centella asiatica (L.) Urban) Syahreza, Daffa Rizki; Tambunan, Efrida Pima Sari; Idami, Zahratul
BEST Journal (Biology Education, Sains and Technology) Vol 6, No 2 (2023): September 2023
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v6i2.8055

Abstract

Uji secara molekuler dilakukan dengan menentukan satu penanda yang tepat untuk menganalisis beberapa kajian dalam bidang molekuler. Uji secara molekuler tumbuhan dilakukan menggunakan penanda gen rbcL (Ribulose-1,5-biphosphate carboxylase/oxygenase large subunit gene). Tumbuhan pegagan (Centella asatica (L.) Urban) merupakan herba yang pada umumnya hidup secara liar di tempat terbuka seperti padang rumput, kebun, tepi jalan, dan tepi parit. Tempat tumbuh pegagan seperti dataran tinggi, dataran rendah, dan pesisir pantai sangat mempengaruhi perubahan dari segi molekuler. Tujuan penelitian ini untuk mengetahui bagaimana karakteristik molekuler, keragaman molekuler (keragaman genetik) dan hubungan filogenetik tumbuhan pegagan (Centella asiatica (L.) Urban) dengan menggunakan gen rbcL. Penelitian ini memiliki tahapan yaitu koleksi sampel, isolasi DNA, amplifikasi DNA dengan gen rbcL, elektroforesis, sekuensing, dan analisis data dengan MEGA 11. Hasil penelitian dari amplifikasi menggunakan gen rbcL pada DNA genom Centella asiatica (L.) Urban yang diteliti menghasilkan panjang amplikon kurang lebih 600 bp. Pada penyatuan sekuens yang konsesus pada sampel mendapatkan sekuens dengan panjang D1 567 bp dan D2 562 bp. Pembentukan pohon filogenetik menggunakan metode Neighbor-Joining dan model perhitungan Kimura-2-Parameter menunjukkan Centella asatica (L.) Urban termasuk dalam kelompok monofiletik. Hasil analisis keragaman molekuler Centella asatica (L.) Urban menunjukkan bahwa pada seluruh sampel yang dikoleksi dari Desa Pantai Labu Pekan Kabupaten Deli Serdang dan Jl. Air Bersih Ujung Kota Medan memiliki keragaman molekuler. Maka dari itu, dapat disimpulkan bahwa gen rbcL menghasilkan barcode DNA dapat digunakan sebagai metode untuk mengidentifikasi Centella asatica (L.) Urban secara molekuler dan sangat efisien dalam menentukan hubungan kekerabatan dengan spesies yang lainnya.
Harmonization of State, Custom, and Islamic Law in Aceh: Perspective of Legal Pluralism Djawas, Mursyid; Nurdin, Abidin; Zainuddin, Muslim; Idham, Idham; Idami, Zahratul
Hasanuddin Law Review VOLUME 10 ISSUE 1, APRIL 2024
Publisher : Faculty of Law, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/halrev.v10i1.4824

Abstract

Indonesia recognizes several legal systems i.e., state law, customary law, Islamic law, and international law. Islamic sharia in Aceh is part of legal pluralism in Indonesia. This study aims to discuss the application of Islamic sharia from the perspective of legal pluralism. This normative legal study employed a legal pluralism theory analysis. Legal pluralism is a theory that views law not only as positive or written law made by the state but also as a recognition of the legal reality that exists in a pluralistic society. The study collected data by means of a literature review. Findings revealed that Islamic sharia in Aceh as part of the recognition of the concept of legal pluralism has been implemented well in the context of state law, custom, and Islamic law. The state has provided juridical legitimacy through laws in the context of legal substance and judicial institutions or sharia courts. Likewise, revitalization has also occurred in traditional institutions in terms of customary law and customary justice. The argument emphasized in this study is that Islamic sharia in the context of legal pluralism has succeeded in manifesting legal harmonization between the state, custom, and Islamic law
Hubungan Kekerabatan Kepiting Rajungan (Portunus sp.) Berdasarkan DNA Barcoding di Perairan Percut Sei Tuan Kabupaten Deli Serdang Zuhra, Arini; Manalu, Kartika; Idami, Zahratul
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4834

Abstract

Rajungan crab (Portunus sp.) is one type of crab that has a fairly high commodity value, this species has a fairly wide distribution covering the Indo-Pacific Ocean and also offshore, this crab has an important role in ecological stability. Portunus sp. has a more attractive appearance compared to other crabs. The purpose of this study is to look at the (dominant) crab species found in January (west wind season) in the waters of Percut Sei Tuan based on the genes CO1, and phylogenetics (kinship relationship) of Portunus sp. in the waters of Percut with Portunus sp. in NCBI or Genebank. The method used in this study is DNA isolation on specimens using the Favorgen kit locus of the CO1 gene in the genome of the Portunus sp. amplified by PCR technique with LCO1940 (forward) and HCO2198 (reverse) primers. The results of the research that have been carried out have found 3 species from the genus Portunus, namely P. sanguinolentus, P. trituberculatus and P. pelagicus. Of the three species found in the Percut Sei Tuan Waters, P. trituberculatus and P. pelagicus have a closer kinship relationship compared to P. sanguinolentus, based on the percentage of AT content and GC content, the AT content is higher at 61.03% which means that the three species have primitive properties. The genetic distance between P. sanguinolentus and P. trituberculatus is closer (19.32) compared to P. pelagicus. Based on the phylogenetic tree reconstruction, P. trituberculatus and P. sanguinolentus are closer to P. pelagicus with the Neighbor-Joining method and the Kimura-2-Parameter calculation model. Thus, it can be concluded that DNA barcoding using the CO1 gene locus can be used as a method in identifying Portunus sp. molecularly and kinship relationships with other species.
ANALISIS MOLEKULER KERANG (Anadara sp.) MENGGUNAKAN MARKER GEN CYTOCHROME OXYDASE SUBUNIT 1 (CO1) DI PERAIRAN PERCUT SEI TUAN Dwi Pratiwi, Anggrainy; Idami, Zahratul; Manalu, Kartika
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4835

Abstract

Anadara sp. clams are very popular clams among the community. Anadara sp. also usually live in waters with muddy and sandy surfaces. DNA barcoding is a barcode sequence to identify unknown species. CO1 is a molecular marker commonly used in molecular research on animals. The purpose of this study was to determine the species of Anadara sp. clams found in Percut Sei Tuan Waters using gene markers, the diversity of Anadara sp. clams using CO1 Gene Markers, and the kinship of Anadara sp. clams found in Percut Sei Tuan Waters. This study has 4 stages, namely DNA isolation using the Favorgen kit, PCR amplification, electrophoresis and sequencing. The DNA sequencing results were analyzed using the MEGA 11 application. The results showed that of the three samples that had been analyzed using the CO1 gene marker obtained using BLAST and NCBI, namely the Anadara granosa, Anadara antiquata, and Anadara gubernaculum samples. With the calculation results of higher AT content (28.9%) and lower GC content (20.12%) which states that the species is more primitive. In the pairwise distance matrix, the samples of Anadara antiquata (Percut Sei Tuan) and Anadara granosa (Percut Sei Tuan) which are in the same clade with different sub clades have a genetic distance ranging from 111.45 to 213.77. The phylogenetic tree also shows that the three samples have a close kinship relationship.
IDENTIFIKASI DNA BARCODING TUMBUHAN BALAKKA (Phyllanthus emblica L.) BERDASARKAN LOKUS GEN rbcL Idami, Zahratul
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.5145

Abstract

This study aims to identify DNA barcodes with the rbcL gene locus and reconstruct the phylogenetic relationship of the Balakka plant with species belonging to the genus Phyllanthus. This research includes experimental research by isolating DNA, amplification DNA using rbcL gene as primer, electrophoresis and sequencing. DNA was isolated with a commercial kit, amplification was carried out using the Polymerase Chain Reaction method. Sequence results and phylogenetic tree reconstruction were analyzed by Bioinformatics (MEGA 11). The results showed that the number of amplicons from the consensus of the two primers on the balakka plant sample was 557 bp. The phylogenetic tree of the Balakka sequence formed a sister clade with Phyllantus emblica KY9888320.1 with 66% similarity, and P. emblica grouped together, but still had common ancestor branches with other species of the Phyllantus genus using the Neighbor-Joining method, and Kimura 2-parameter calculations.
Pendampingan Masyarakat Sekolah dalam Meningkatkan Kesadaran Menjaga Kebersihan Lingkungan Idami, Zahratul; Rifa'ah, Sirriyatur; Muntiza, Rizka; Navisa A, Alya; Nurisa, Vina; Ramadani, Fitria
Catimore: Jurnal Pengabdian Kepada Masyarakat Vol 3 No 1 (2024): Catimore: Jurnal Pengabdian kepada Masyarakat
Publisher : LSM Catimore dan Sahabat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56921/cpkm.v3i1.166

Abstract

Healthy and clean environment is something that must be initiated for the survival of future generations so that it is not threatened by the consequences of irresponsible human behaviour. This community service is inspired by the lack of students’ awareness in maintaining environmental cleanliness and the lack of cleaning facilities at MTs MIM Langsa. The procedure of this assisting activity is carried out through 3 stages, namely: planning, implementing, and reflecting, which begins with field observation to the target school. This potential-based community service activity is in the form of counseling/socialization and mentoring on school environmental hygiene. Socialization activities targeting OSIM administrators of MTs MIM Langsa as role models are expected to motivate other students to always maintain school cleanliness. Meanwhile, other activities include painting garbage cans and providing posters with the theme of environmental cleanliness. The provision of cleaning facilities in the form of garbage cans in several places in the school environment has received positive responses and support from the school community, one of which is showed by students who responsibly do class pickets and do not litter.
Co-Authors A. Rani, Faisal Abdul Wahab Abdi, Abdul Wahab Abidin Nurdin Aisyah Hutasuhut, Melfa Alfi Rahman Ali Afwanudin Ali Akbar Alkaf, Muhammad Amalia Mukhtar Ashari, Maya Asri Gani Astuti, Astri Widia Azzahra, Fathia Azzahrawi Azzahrawi Balqish, Annisa Br Sitorus, Julreda Banu Br Tarigan, Rindi Atika Cut Khairunnisak Dakiani diana, Rahma Doly, Raja Dur , Sajaratud Dwi Pratiwi, Anggrainy Eddy Purnama Ellianti, Ellianti Erdiwansyah Erdiwansyah Fanisah Labibah Fatiani Manik Fattara, Fajar Putra Febrian, Fadly Ferdy Saputra Fita Nelyza Hadi Iskandar Hafsa, Sri Sholeha Hara, Nina Tiya Harahap, Atika Rahmah Harnita Harnita Hasra, Barratun Nisa Helmi Helmi Husna, Nina Hayatul Husnarika Febriani, Husnarika Husni Djalil Husni Husni Hutasuhut, Melfa Aisyah Ichsan Maulana Ida Muliawati Idham Idham Ihdi Karim Makinara Ihyannisak Zain Israr Hirdayadi Kasmawati Kasmawati Keumala, Meta Khaira MS, Nazlil Kurniasy, Dessy Kusumapradja, Rokiah Lestari, Revi Lubis, Habib Jailani M Ikhsan M. Hardiansyah M. Idris M. Irham M. Zaki Mahfud Mahfud Mahidin Mahidin Mahriza, Rita Manalu, Kartika Manulu, Kartika Mariana Mariana Masturah, Siti Ghaisani Maulidian, Muhammad Okta Ridha Meutia, Cut Intan Muazzin Muazzin muhammad rizky, muhammad Muhammad Tirta Rizky Mukhlis Mukhlis Mukhlis Mukhlis Mumtaz, Raydina Muntiza, Rizka Mursyid Djawas Muslim Zainuddin N. Nazaruddin Nadila, Evi Navisa A, Alya Nova Fahradina Novianti, Mutiara Nugroho, Muhammad Agung Nurisa, Vina Nurul Fadhilah Pocut Navieza Pratama, Yuda Rizky Puspita, Siti Neni Qudwatin Nisak M. Isa Rahmadina, Rahmadina Rahmadsyah, Rangkuti Rahmah Johar Ramadani, Fitria Ramayati, Ike Rangkuti, Ana Rahmawati Rangkuti, Rahmadsyah Rangkuti, Rahmatsyah Raza, Mohd Hamid Rifa'ah, Sirriyatur Riski Afrial Ritonga, Desi Rohaizati, Ulya Rosifa, Gita Dewi Rossya, Nadila Rizki Rusdianda Rusdianda Saifani, Meutia Sajaratud Dur, Sajaratud Salsabila, Nada Shafrida Wati, Shafrida Shafridawati Shella Namira Wardia Shinta Seftyana Silalahi, Ali Affan Simbolon, Wira Hariati Siregar, Elsa Manora Suhaimi Suhaimi Suhery, Dedy Syahputra, Akbar Syahreza, Daffa Rizki Syahrizal Abbas Tambunan, Efrida Pima Sari tiara dewi Utami, Mutiara Alya Wahidah Wahidah Wahidah Wandini, Dwi Febiyanti Yanis Rinaldi Yunita Yunita Yusrizal Hasbi Yusvita, Diana Zaiyana Putri, Zaiyana Zakaria Zakaria Zuhra, Arini Zulyani Mahmud