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KEANEKARAGAMAN JENIS FAMILI URTICACEAE DI HUTAN DESA BUKUM KECAMATAN SIBOLANGIT KABUPATEN DELI SERDANG SUMATERA UTARA Ramayati, Ike; Hutasuhut, Melfa Aisyah; Idami, Zahratul
Eksakta : Jurnal Penelitian dan Pembelajaran MIPA Vol 7, No 2 (2022): Eksakta : Jurnal Penelitian dan Pembelajaran MIPA
Publisher : Fakultas Keguruan Dan Ilmu Pendidikan, UM-Tapsel

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/eksakta.v7i2.240-248

Abstract

The Urticaceae family is herbaceous, semi-shrub, climbing (liana), small trees, and shrubs that have important ecological and economic roles. This research was conducted to determine the composition and structure, diversity, and utilization of Urticaceae family plants in the Forest of Bukum Village, Sibolangit District, Deli Serdang Regency, North Sumatra. This research is an exploratory survey using the quadratic method. The plots were purposive sampling as many as 10 plots measuring 10m x 10m. There are 25 species of plants in the Urticaceae family with 547 individuals. The most common type of plant found is Elatostema sessile JRForst. & G. Forst. Highest importance index on Elatostema sessile JRForst. & G. Forst. (INP 91,30%). The plant diversity index of the Urticaceae family is moderate with H' 2.64 which is included in the stable community.
KEANEKARAGAMAN DAN POLA SEBARAN FAMILI ARACEAE DI HUTAN DESA BUKUM KECAMATAN SIBOLANGIT KABUPATEN DELI SERDANG SUMATERA UTARA Diana, Rahma; Hutasuhut, Melfa Aisyah; Idami, Zahratul
Eksakta : Jurnal Penelitian dan Pembelajaran MIPA Vol 7, No 2 (2022): Eksakta : Jurnal Penelitian dan Pembelajaran MIPA
Publisher : Fakultas Keguruan Dan Ilmu Pendidikan, UM-Tapsel

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/eksakta.v7i2.249-256

Abstract

The family Araceae, the taro family, has a terrestrial habitus, for better or for worse, submerged or submerged in water. Araceae is also very abundant and diverse in wet tropical areas such as the Bukum Village Forest area. This study aims to determine the types of the Araceae family, the diversity index and the distribution pattern of the Araceae family in the Forest of Bukum Village, Sibolangit District, Deli Serdang Regency, North Sumatra which was carried out in December 2021 - February 2022. The method used was exploratory with the technique of making square plots. by purposive sampling on 2 transects, each of which has 8 plots of observations. The results of the study were 24 species from the Araceae family with the most common species being Schismatoglottis calyptrata (Roxb) with 157 individuals. There are at least 4 species of individuals, namely Aglaonema commutatum Schott, Aglaonema nebulosum N.E.Br., Homalomena rostrata Griff. and Syngonium podophyllum Schott by 2 individuals. The diversity index of transect I was 2.00 and transect II was 2.36. The diversity index data shows moderate environmental diversity, because the productivity is quite high, the ecosystem condition is balanced and the ecological pressure is moderate. The morisita index on transect I of 1.59 shows the pattern of distribution is moderate, this occurs due to uneven (uniform) abiotic conditions although in a narrow location. The morisita index on transect II of 0.98 indicates an even distribution pattern, due to individual competition which encourages the distribution of space evenly. 
VARIASI GENETIK IKAN KERAPU (Epinephelus sp) Di TPI GABION MEDAN BELAWAN BERDASARKAN DNA MITOKONDRIA Hasra, Barratun Nisa; Idami, Zahratul; Manalu, Kartika
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 2 (2025): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue2page203-210

Abstract

Background: Genetic variation can share data on genetic diversity in fish populations. mtDNA is used to determine the kinship between grouper fish originating from TPI Gabion Medan Belawan. This study aims to obtain the latest genetic variations found in grouper populations (Epinephelus sp.), analyze for the kinship relationship between grouper populations (Epinephelus sp.), determine for the kinship relationship between grouper fish from TPI Gabion Medan Belawan and grouper fish that already exist in NCBI.Methods: This study used experimental methods.Results: The results of research on species of the genus Epinephelus namely Epinephelus fuscoguttatus, Epinephelus sexfasticus, and Epinephelus areolatus obtained by processing the sequencing data by analyzing nucleotide characters using the Mega 11 application. All three samples have low GC Content, it is said that all three samples have primitive properties. All three samples have the same genetic distance because they have a genetic distance value (Pairwise distance) below 3%.Conclusion: Reconstruction of phylogenetic trees shows that grouper kinship (phylogenetics) originating from TPI Gabion Medan Belawan with grouper fish that already exist in NCBI have close kinship and common ancestor
DNA BARCODE KERANG KEPAH (Polymesoda Sp.) DI BAGAN PERCUT DAN DANAU SIOMBAK SUMATERA UTARA MENGGUNAKAN GEN CYTOCHROME OXIDASE SUB UNIT 1 (CO1) Utami, Mutiara Alya; Idami, Zahratul; Manalu, Kartika
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 2 (2025): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue2page194-202

Abstract

Background: DNA Barcode is a molecular technique using short sequences of nucleotide bases that aims to identify and classify living things. This study aims to obtain nucleotide characters from DNA barcode results from kepah clams using CO1 genes in Sumatran waters, to determine the results of molecular identification of species of kepah clams (Polymesoda sp.) found in North Sumatra waters, and to determine the kinship relationships of kepah clam species (Polymesoda sp.) in North Sumatra waters. Samples were obtained in Bagan Percut and Lake Siombak, carried out from July to August 2023 at the Biomolecular Laboratory, Medan State University. Methods: The method used is to use DNA barcodes with CO1 genes. Results: The results of research of species of the genus Polymesoda sp. namely Polymesoda erosa and Polymesoda expansa obtained from processing sequencing data by analyzing nucleotide characters using the Mega 11 application, namely variations in the percentage of AT content (62.69%), GC content (37.56%) for Polymesoda erosa species and AT content (62.42%), GC content (38.64%) for Polymesoda expansa species, which means both species have primitive properties. Molecular identification of the genus Polymesoda sp. obtained from the results of DNA sequence samples along 369 bp so that it is suitable to be used as DNA barcodes. The genetic distance between the species studied is Polymesoda erosa (0.000) and Polymesoda expansa (0.920) which means they have a close genetic distance. Conclusion: Phylogenetic tree reconstruction carried out using the Neighbour Joining method shows that between the two species Polymesoda erosa (Bagan Percut) and Polymesoda expansa (Lake Siombak) have a close kinship and come from a common ancestor.
ANALISIS VARIASI GENETIK PADA KERANG Anadara MENGGUNAKAN MARKER RAPD (Random Amplified Polymorphic DNA) Siregar, Elsa Manora; Idami, Zahratul; Dur , Sajaratud
JURNAL BIOSENSE Vol 8 No 2 (2025): Edisi April 2025
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v8i2.5136

Abstract

Di Indonesia, kerang Anadara dikenal dengan nama umum kerang bulu dan kerang darah. Informasi mengenai variasi genetik spesies ini masih terbatas. Penelitian ini bertujuan untuk menganalisis sidik jari DNA kerang Anadara guna mengidentifikasi polimorfisme dan variasi genetik antar individu dan antarpopulasi. Penelitian ini menggunakan metode analisis keragaman genetik pada populasi kerang Anadara menggunakan aplikasi teknik sidik jadi DNA, jenis primer yang efektif untuk mengidentifikasi pola polimorfisme, dan menentukan kekerabatan yang terbentuk dari teknik sidik jari DNA menggunakan marker Random Amplified Polymorphic DNA (RAPD) menggunakan enam primer yaitu, OPD 20, OPC 02, OPA 2, OPA 13, UBC 101 dan UBC 456. Hasil penelitian menunjukkan bahwa teknik RAPD berhasil mengidentifikasi variasi genetik yang signifikan antar individu kerang Anadara. Total 27 pita DNA teramati dengan ukuran berkisar antara 200-3000 pasang basa. Tingkat polimorfisme mencapai 100% dengan rata-rata 5-8 pita polimorfik perprimer. Terdapat satu primer monomorfik dan satu primer tidak ter amplifikasi. Tingkat kemiripan genetik antar individu bervariasi dari 0,68 hingga 1.00, mengindikasikan keragaman genetik yang substantial. Dendogram hasil analisis menunjukkan pengelompokan yang jelas berdasarkan hasil yang di dapatkan dalam PCR-RAPD. Berdasarkan hasil penelitian yang telah dilakukan, dapat disimpulkan bahwa metode RAPD efektif dalam mengungkap variasi genetik kerang Anadara. Temuan ini memberikan kontribusi penting dalam memahami struktur genetik spesies.
ANALISIS POLIMORFIK GENETIK DNA IKAN MAS KOKI GENUS Carrasius MENGGUNAKAN METODE RAPD (Random Amplified Polymorphic DNA) Fadhilah, Nurul; Idami, Zahratul; Dur, Sajaratud
JURNAL BIOSENSE Vol 8 No 2 (2025): Edisi April 2025
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v8i2.5137

Abstract

Ikan mas koki (Carrasius auratus) adalah ikan memiliki ciri khas yang tersendiri. Keunikan dan keragamannya dapat diukur dari warna yang cemerlang, bentuk dan kelengkapan fisik. Tujuan penelitian ini adalah untuk mengetahui primer yang efektif, polimorfik genetik DNA dan kekerabatan pada Carrasius auratus yang terdapat di pembudidayaan ikan hias, Binjai Utara dengan menggunakan metode RAPD dengar enam primer yaitu OPA 13, UBC 101, UBC 456, OPD 20, OPA 02 dan OPC 02. Tahapan yang dilakukan pada penelitian ini diantaranya isolasi DNA menggunakan Kit Favorgen, amplifikasi PCR, dan elektroforesis. Hasil penelitian menunjukkan primer yang teramplifikasi dan menghasilkan pita polimorfik adalah primer OPA 13, OPA 02 dan OPD 20. Jumlah pola pita dari hasil amplifikasi primer tersebut sebanyak 32 pita dengan panjang basa yang berkisar antara 100 bp – 1500 bp. Pita yang muncul kemudian diterjemahkan kedalam data biner dan dianalisis dengan program NTSYS versi 2.02. Menunjukkan bahwa kekerabatan terdekat adalah pada ikan mas koki jenis Ryukin dan Oranda dengan memiliki koefisien 0,74 atau sebesar 74%, sedangkan kekerabatan jauh pada Ikan Mas Koki Rachu dan Teleskop dengan koefisien sebesar 0,75 atau sebesar 75%. Dari data hasil panjang pita 900 bp, 600 bp, 400 bp, 300 bp 200 bp dan 100 bp dari komponen pertama memiliki peran penting dalam pengelompokkan Carrasius auratus dalam dendogram.
Perceiving the Impact of Visual Media: A Phenomenological Study on Teacher Experiences in Teaching English to Elementary School Hafsa, Sri Sholeha; Wahidah, Wahidah; Idami, Zahratul
Al-Azkiya: Jurnal Pendidikan MI/SD Vol 9 No 1 (2024): Al-Azkiya: Jurnal Pendidikan MI/SD
Publisher : the Study Program of Education for Islamic Elementary School Teachers (Undergraduate)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32505/azkiya.v9i1.8303

Abstract

Teaching English to children is crucial. English plays a significant role internationally, providing access to information and technology. Effective teaching for young learners requires good planning, suitable methods, and appropriate materials. The purposes of this study were to (1) identify the visual aids that English teachers at MIN 2 Langsa utilize to teach English to primary pupils, and (2) investigate the teacher's experiences with these aids. Data collection was done in December 2023 through a fifth-grade English teacher at MIN 2 Langsa. The phenomenological approach was combined with a qualitative method in this study. An interview and an observation served as the instruments. The purpose of the observation was to see how the fifth-grade pupils at MIN 2 Langsa's teachers using media, and the interview was given to the English teacher to learn more about the teacher's expertise with visual media. The results of this study demonstrated that the types of media that teachers used to teach English included whiteboards, English textbooks, dictionaries, posters, pictures, games, and other objects that were nearby. The interview with the teacher revealed that, depending on the subject, she employed visual aids to simplify the English lesson and help the pupils understand it better.
Teacher and EFL Primary School Students' Perspectives on the Shadowing Technique for Pronunciation Instruction Wati, Shafrida; Idami, Zahratul
Al-Azkiya: Jurnal Pendidikan MI/SD Vol 9 No 2 (2024): Al-Azkiya: Jurnal Pendidikan MI/SD
Publisher : the Study Program of Education for Islamic Elementary School Teachers (Undergraduate)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32505/azkiya.v9i2.9656

Abstract

This study elaborates the perspectives of teachers and primary school students learning English as a Foreign Language (EFL) regarding the application of the shadowing technique for pronunciation. Scholars have acknowledged shadowing as an effective technique for improving learners' pronunciation, where pupils replicate speech as carefully and promptly as possible following a speaker. This qualitative study was carried out at MIN 2 Langsa throughout the academic year of 2022-2023. Subject of the research was an English teacher and 30 fifth-grade pupils selected purposively. Data were gathered through semi-structured interviews with an EFL teacher and focus group discussions with the primary school students, followed by classroom observations to provide a deeper comprehension of the actual use of the method. Thematic analysis was employed to identify key patterns and themes in participants' responses. Results revealed that teacher considered the technique effective for enhancing students' speech accuracy, rhythm, and intonation. It offers students an immediate opportunity to correct their pronunciation and actively involves them in learning English, particularly in terms of pronunciation. The learners primarily viewed shadowing activity as an enjoyable exercise, where they practiced English chorally. This technique fosters students' confidence in the English classroom by alleviating individual pressure, while all participants learn and practice English together. Thus, it can be a powerful tool for enhancing young learners' pronunciation in an engaging way. English teachers might employ the technique as an alternative strategy to engage young learners in foreign language acquisition, particularly in pronunciation.
Vegetable Pesticides and Planting Spattern Againt Grayak Caterpillar (Spodoptera exigua) Attacks on long Beans (Vigna Sinensis L.) Pratama, Yuda Rizky; Idris, M.; Idami, Zahratul
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9368

Abstract

: The armyworm (Spodoptera exigua) is one of the most common plant pests that lower plant yields.  The purpose of this study is to ascertain how armyworm (Spodoptera exigua) attacks on long bean plants are impacted by botanical pesticides and planting distance.  This study employs experiments and is quantitative in nature.  In this investigation, papaya leaf trials and garlic peels were used as plant insecticides in covert studies.  The one-way ANOVA test was the statistical method employed in this investigation. The findings demonstrated that while the botanical concentration had no effect on plant height or attack severity, it significantly affected the percentage of attacks and the leaf area index.  Plant height, attack intensity, attack %, and leaf area index were not significantly affected by planting distance in long bean plants.  The study's conclusions include the need for more research on plant insecticides that use plant leaves to fend against attacks, particularly in lowering the incidence of armyworm attacks.
Analisis Kekerabatan Menggunakan Metode Fingerprint DNA pada Ikan Sepat (Ordo Anabantiformes) di Perairan Kecamatan Kualuh Leidong Ritonga, Desi; Idami, Zahratul; Manulu, Kartika
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.550

Abstract

Gourami is a type of freshwater fish that is widely distributed in Indonesia, including in the waters of Kualuh Leidong District, North Sumatra. However, the kinship relationship between gourami species in the region has not been widely studied. Therefore, this study aims to analyze the kinship relationship of several gourami species found in the waters of Kualuh Leidong using the DNA fingerprint method. This study uses a molecular characterization method with several stages, namely collecting fish samples, isolating and extracting DNA, amplifying DNA using Polymerase Chain Reaction (PCR) with several primers, electrophoresis, and analyzing data using NTSYS software. Sampling was carried out by collecting specimens from the waters of Kualuh Leidong, including four types of gouramis, namely Trichopodus trichopterus, Trichopodus pectoralis, and Helostoma temmincki. The electrophoresis results showed that the DNA of the gourami fish could be amplified with primers OPA 02, OPC 02, and UBC 456, but not amplified with primer OPA 20. The DNA banding pattern obtained consisted of polymorphic and monomorphic bands with varying locus sizes. Jaccard coefficient analysis showed a high level of kinship (>70%), with the species Trichopodus pectoralis having a similarity of 0.78, Trichopodus trichopterus 0.76, and Helostoma temmincki 0.71. The results of this study can be used as a basis for the conservation and management of gourami fish resources in the waters of North Sumatera.
Co-Authors A. Rani, Faisal Abdul Wahab Abdi, Abdul Wahab Abidin Nurdin Alfi Rahman Ali Afwanudin Ali Akbar Alkaf, Muhammad Amalia Mukhtar Ashari, Maya Asri Gani Astuti, Astri Widia Azzahrawi Azzahrawi Br Tarigan, Rindi Atika Cut Khairunnisak Dakiani diana, Rahma Doly, Raja Dur , Sajaratud Dwi Pratiwi, Anggrainy Eddy Purnama Ellianti, Ellianti Erdiwansyah Erdiwansyah Fattara, Fajar Putra Fita Nelyza Hafsa, Sri Sholeha Hara, Nina Tiya Harahap, Atika Rahmah Harnita Harnita Hasra, Barratun Nisa Helmi Helmi Husna, Nina Hayatul Husnarika Febriani, Husnarika Husni Djalil Husni Husni Hutasuhut, Melfa Aisyah Ichsan Maulana Ida Muliawati Idham Idham Ihdi Karim Makinara Ihyannisak Zain Israr Hirdayadi Kasmawati Kasmawati Keumala, Meta Khaira MS, Nazlil Kurniasy, Dessy Kusumapradja, Rokiah Leni Widiarti, Leni Lismalinda Lubis, Habib Jailani M Ikhsan M. Hardiansyah M. Idris M. Irham M. Zaki Mahfud Mahfud Mahidin Mahidin Manalu, Kartika Manulu, Kartika Mariana Mariana Masturah, Siti Ghaisani Maulidian, Muhammad Okta Ridha Muazzin Muazzin muhammad rizky, muhammad Mukhlis Mukhlis Mukhlis Mukhlis Mumtaz, Raydina Mursyid Djawas Muslim Zainuddin Nadifa, Dalilla Nadila, Evi Nasution, Niska Maulida Ningrum, Nurlian Augustin Nova Fahradina Novianti, Mutiara Nugroho, Muhammad Agung Nurul Fadhilah Pocut Navieza Pratama, Yuda Rizky Puspita, Siti Neni Qudwatin Nisak M. Isa Rahmadina, Rahmadina Rahmadsyah, Rangkuti Rahmah Johar Ramayati, Ike Rangkuti, Ana Rahmawati Rangkuti, Rahmadsyah Rangkuti, Rahmatsyah Riski Afrial Ritonga, Desi Rohaizati, Ulya Rosifa, Gita Dewi Rossya, Nadila Rizki Rusdianda Rusdianda Saifani, Meutia Sajaratud Dur, Sajaratud Salsabila, Nada Shafrida Wati, Shafrida Shafridawati Shella Namira Wardia Shinta Seftyana Silalahi, Ali Affan Simbolon, Wira Hariati Siregar, Elsa Manora Suhaimi Suhaimi Suhery, Dedy Syahputra, Akbar Syahreza, Daffa Rizki Syahrizal Abbas Tambunan, Efrida Pima Sari tiara dewi Utami, Mutiara Alya Wahidah Wahidah Wahidah Wandini, Dwi Febiyanti Yanis Rinaldi Yunita Yunita Yusvita, Diana Zaiyana Putri, Zaiyana Zakaria Zakaria Zuhra, Arini Zulyani Mahmud