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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) METAMORFOSA Journal of Biological Sciences Kolaboratif AL KAUNIYAH Jurnal Biologi Universitas Andalas Jurnal Riset Kimia Biocelebes Kultivasi Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Agrotek Tropika BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Hilirisasi IPTEKS (JHI) Agro Bali: Agricultural Journal BERNAS: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian kepada Masyarakat Nusantara Bioscientist : Jurnal Ilmiah Biologi Dharma: Jurnal Pengabdian Masyarakat Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat Eduvest - Journal of Universal Studies AGRIC Jurnal Biosilampari: Jurnal Biologi
Zozy Aneloi Noli
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Andalas
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Pengaruh Lama Perendaman pada Biopriming Padi Anak Daro Menggunakan Bacillus subtilis dan Trichoderma harzianum Noli, Zozy Aneloi; Alamsjah, Feskaharny; Rahmayati, Riesca Salsabilah
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10987

Abstract

The majority of Indonesian people consume rice as a staple food. According to data from the Central Statistics Agency (2022), rice production in West Sumatra reached 1,482,996.01 tons in 2019 and continued to decline until 2021, total rice production in West Sumatra was only 1,317,209.38 tons. This is inversely proportional to the population of West Sumatra which increases every year, so efforts need to be made to increase rice production. One of them is the biopriming technique to increase seed vigor. Priming is hydration carried out slowly which aims to ensure that the seed's water potential reaches balance and activates metabolism in the seed before the seed germinates. One of them is biopriming, which is a seed priming technique using biological agents in the form of beneficial microorganisms. Several factors influence the success of the priming technique, one of which is the soaking time. The right soaking time provides a deeper opportunity to absorb water to stimulate the seeds to germinate. This research aims to determine the effect of soaking time on biopriming of the local West Sumatran rice variety "Anak Daro" using Bacillus subtilis and Trichoderma harzianum on the germination of the Anak Daro rice variety. Completely Randomized Design (CRD) factorial pattern with 2 factors with 3 replications. Factor A is a biopriming agent, namely A1 (no microbes), A2 (Bacillus subtilis), and A3 (Trichoderma harzianum). Factor B is the length of soaking, namely B1 (24 hours), B2 (48 hours), and B3 (72 hours). The results showed that Bacillus subtilis and Trichoderma harzianum had a significant effect on maximum growth potential and germination, while soaking time had a significant effect on maximum growth potential and vigor index.
Micropopagation of Cryptocarya massoy (Oken) Kosterm. Trough Optimizing Callus Induction with Benzylaminopurine (BAP) Supplementation Hanafi, Muhammad; Noli, Zozy Aneloi; Idris, M.; Hany, Iga Permata
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.13852

Abstract

This study aimed to evaluate the effect of BAP concentrations on explant response and callus formation in Massoia. The experiment was arranged in a Completely Randomized Design (CRD) with three BAP concentrations: 0.5 mg/L, 1.0 mg/L, and 1.5 mg/L, each with 24 replications. The culture medium used was Murashige and Skoog (MS), and observations were carried out for 7 days after planting. Parameters observed included the percentage of viable explants, browning, contamination, average callus formation, callus color, and texture. Data were analyzed using analysis of variance (ANOVA) at a 5% significance level. The results showed that 0.5 mg/L BAP yielded the highest explant survival rate (54%), the lowest browning rate (13%), and 35% contamination. Meanwhile, 1.5 mg/L BAP produced the highest average callus formation (0.21) with compact texture and brown coloration. Although not statistically significant, BAP concentrations affected explant viability and callus formation. A concentration of 0.5 mg/L tended to be optimal for maintaining explant viability, while 1.5 mg/L was more effective in inducing callus. These findings provide a preliminary basis for optimizing Massoia tissue culture techniques.
Pengaruh Benzil Aminopurin (BAP) terhadap Induksi Tunas dari Nodus anggrek Dendrobium mussauense Media, Media; Noli, Zozy Aneloi
Jurnal Biologi Universitas Andalas Vol 12 No 1 (2024)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.12.1.35-41.2024

Abstract

Dendrobium mussauense has attractive flowers and potential as a parent of a hybrid orchid. The color of this species is cream with violaceous purple venations on the labellum. D. mussauense is an orchid species listed as vulnerable in IUCN. This species is not located in protected areas nor known to be conserved ex-situ. Clonal propagation is an important biotechnological tool for the conservation of orchids. Thin cell layer (TCL) is a micropropagation method using thin-sized explants that facilitate the diffusion of nutrients to tissues. This study examined induction shoot through two explant cutting techniques, namely TCL and non-TCL explant, at various concentrations of BAP. The non-TCL explant was more effective than the TCL explant in inducing the shoot of D. mussauense. The half-strength Murashige and Skoog (MS) medium supplemented with 0.9 mg.L-1 BAP was a suitable concentration to induce the shoot of D. mussauense through non-TCL explant. The average shoot length was 1,8 cm, and the average number of shoots was 5/explant.
Shoot Induction of Dendrobium lasianthera J.J.Smith with Several Types of Cytokinins through In Vitro Culture Arli, Naura Muthiah; Noli, Zozy Aneloi
Jurnal Penelitian Pendidikan IPA Vol 10 No 4 (2024): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i4.5324

Abstract

Dendrobium lasianthera (Orchidaceae) is one of the potential plants as ornamental plants because it has beautiful and long-lasting flowers. Propagation of orchid plants through in vitro culture technique has been widely done to meet market demand. One of the supporting factors in plant propagation through in vitro culture techniques is growth regulators. Cytokinin is one of the growth regulators commonly used in plant propagation, including orchids. The study aims to determine the effect of several cytokinins and find the best type for shoot induction of Dendrobium lasianthera. The method used a Completely Randomized Design (CRD) with 4 treatments and 6 replications with several types of cytokinis (control; BAP 1.2 mg/L, Kinetin 2 mg/L, and Thidiazuron 1 mg/L) in Murashige & Skoog (MS) media. The results showed that several cytokinins significantly affected the number of shoots, time of shoot appearance, and shoot length of D. lasianthera. Thidiazuron was the best cytokinin for in vitro shoot induction of D. lasianthera
Optimalisasi Konsentrasi Thidiazuron untuk Peningkatan Pertumbuhan Protocorm In Vitro Anggrek Grammatophyllum stapeliiflorum Noli, Zozy Aneloi; Arli, Naura Muthiah; Suwirmen
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9067

Abstract

Cytokinins are growth regulators that play a crucial role in cell division and shoot growth. Thidiazuron, a potent cytokinin, was the focus of our research. The research aimed to determine the effect of several concentrations of Thidiazuron on the protocorm growth of Grammatophyllum stapeliiflorum and to identify the best concentration of Thidiazuron (mg/L) for the protocorm growth of G. stapeliflorum orchids in vitro. The research was conducted from January until March 2022 at the Plant Physiology Laboratory, Universitas Andalas, Padang. The research used a Completely Randomized Design (CRD) with 5 treatments and 5 replications. Several concentrations of Thidiazuron were added to MS media with various concentrations: 0 mg L-1 (A), 0.50 mg L-1 (B), 1 mg L-1  (C), 1.50 mg L-1(D), and 2 mg L-1  (E). The parameters in this research were the percentage of life explants, day of shoot appearance, number of shoots, shoot length, number of leaves, number of roots, and root length. The results showed that Thidiazuron significantly affected the protocorm growth of the G. stapeliiflorum orchid compared to the control treatment. Notably, 2 mg L-1  Thidiazuron emerged as the best concentration for all growth parameters of protocorm G. stapeliiflorum, a finding that could significantly impact our understanding of orchid cultivation and plant physiology.
Co-Authors . Mansyurdin A. Fadhil Desafa Adam Raihan Priambada Adelia, sabbrina Adrial, Rico Afdhal Muttaqin Agusti Apriliani Alimin Mahyudin Alponsin Alponsin Anita Sari Anita Tri Astuti Anthoni Agustien Arif Budiman Arli, Naura Muthiah Asih Maharani Asih, Enda Tarni Asnul Fitria Astuti Aulia Suci Desila Aulia, Annisa Aulya, Nailul Rahmi Ayola Pajrita Ayu Utami Rezki Azharia Khalida Chairul Chairul Chairul Chairul Cleopatra Dahyunir Dahlan De Yudanur, Parissa Anandita Dedi Mardiansyah Delfi, Shyla Aulia Della Amelia Dezi Meutya Dapersal Dian Fitriyani Diana Fadhilah Dilla, Arfa Iskhia Djong Hon Tjong Dola Ratna Yulizar Dwi Pujiastuti Dwi Puryanti Efrizal Efrizal Efrizal Efrizal Eka Muliani Elfans Bawalsyah S.A. Elvaswer Elvina Sari Emelta, Citra Endah Mutia Sari Feby Zulya Femilya Putri Fera Malta Feriska Handayani Ferry Lismanto Syaiful Feskaharny Alamsjah Fiqi Diyona Fira Julia Putri Fitri, Arya Wisata Gian Wulandari Gita Prima Yudha Haldis Alvaro Hany, Iga Permata Helsya Vellarentika Labukti Hesti Dwi Marcellinna Husri Meli Iga Permata Hany Ilham, Kurniadi Imam Taufiq Ivo Octaviani Izmiarti Izmiarti, Izmiarti Julita Julita Kiki Ayunda Putri Kurniadi Ilham Liza Febriani Sukma Lubis, Amelia Sriwahyuni Lufri Lufri M. Ali Shafii M. Idris M. Teguh Dhiya Ulhaq Mairawita Mairawita, Mairawita Maliza, Rita Marta Linda Marta, Fepi Dwi Mayola Arda Media Media Media, Media Meqorry Yusfi Miftah Mussaumi Adi Mildawati Mildawati Mildawati Millania Putri Shayen Millania Putri Shayen Muhammad Azwar Muhammad Hanafi Naura Muthiah Arli Nazhira - Fadhilah Nini Firmawati Nopiyanti, Tita Novia Rika Deli Nur, Fauziah Nurhafitri, Amanda Olly Norita Tetra Pasha, Gusti Ari Afrilya Periadnadi Periadnadi Prima Fauziah Haq Puspita, Ayumi Rizci Puti Khairunnajwa Amar Putra Santoso Putri Aliyyanti Putri Seti Ayu Putri, Mellanie Alia Putri, Suci Indah Rahma Devi Rahmayati, Riesca Salsabilah Ramacos Fardela Resti Rahayu Reswita Reswita Reza Oktavia Riesca Salsabilah Rahmayati Rilwan Efendi Rismayani, Dessy Rizqa Zidna Chairafahmi Rusiati, Anisa Rahman Santhyami Santhyami Shayen, Millania Putri Sherin Dien Salsabila Siagian, Marhamah Solfiyeni Solfiyeni Sri Handani Suci Rahmadani Putri Sumaryati Syukur Suwirmen Suwirmen, Suwirmen Syabila, Hutri Dinda Tesri Maideliza Tesri Maideliza Tiara Tiara Tita Nopiyanti Titiek Rukmini Trengginas Eka Putra Tressa Pratywi Gupitasari Wenny Rahma Gusni Yella Prastika Yuda Yossi Eka Saputri Yulianti, Sisi Zulfi Zulkarnain, Alivia