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Pengaruh Lama Perendaman pada Biopriming Padi Anak Daro Menggunakan Bacillus subtilis dan Trichoderma harzianum Noli, Zozy Aneloi; Alamsjah, Feskaharny; Rahmayati, Riesca Salsabilah
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10987

Abstract

The majority of Indonesian people consume rice as a staple food. According to data from the Central Statistics Agency (2022), rice production in West Sumatra reached 1,482,996.01 tons in 2019 and continued to decline until 2021, total rice production in West Sumatra was only 1,317,209.38 tons. This is inversely proportional to the population of West Sumatra which increases every year, so efforts need to be made to increase rice production. One of them is the biopriming technique to increase seed vigor. Priming is hydration carried out slowly which aims to ensure that the seed's water potential reaches balance and activates metabolism in the seed before the seed germinates. One of them is biopriming, which is a seed priming technique using biological agents in the form of beneficial microorganisms. Several factors influence the success of the priming technique, one of which is the soaking time. The right soaking time provides a deeper opportunity to absorb water to stimulate the seeds to germinate. This research aims to determine the effect of soaking time on biopriming of the local West Sumatran rice variety "Anak Daro" using Bacillus subtilis and Trichoderma harzianum on the germination of the Anak Daro rice variety. Completely Randomized Design (CRD) factorial pattern with 2 factors with 3 replications. Factor A is a biopriming agent, namely A1 (no microbes), A2 (Bacillus subtilis), and A3 (Trichoderma harzianum). Factor B is the length of soaking, namely B1 (24 hours), B2 (48 hours), and B3 (72 hours). The results showed that Bacillus subtilis and Trichoderma harzianum had a significant effect on maximum growth potential and germination, while soaking time had a significant effect on maximum growth potential and vigor index.
Micropopagation of Cryptocarya massoy (Oken) Kosterm. Trough Optimizing Callus Induction with Benzylaminopurine (BAP) Supplementation Hanafi, Muhammad; Noli, Zozy Aneloi; Idris, M.; Hany, Iga Permata
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.13852

Abstract

This study aimed to evaluate the effect of BAP concentrations on explant response and callus formation in Massoia. The experiment was arranged in a Completely Randomized Design (CRD) with three BAP concentrations: 0.5 mg/L, 1.0 mg/L, and 1.5 mg/L, each with 24 replications. The culture medium used was Murashige and Skoog (MS), and observations were carried out for 7 days after planting. Parameters observed included the percentage of viable explants, browning, contamination, average callus formation, callus color, and texture. Data were analyzed using analysis of variance (ANOVA) at a 5% significance level. The results showed that 0.5 mg/L BAP yielded the highest explant survival rate (54%), the lowest browning rate (13%), and 35% contamination. Meanwhile, 1.5 mg/L BAP produced the highest average callus formation (0.21) with compact texture and brown coloration. Although not statistically significant, BAP concentrations affected explant viability and callus formation. A concentration of 0.5 mg/L tended to be optimal for maintaining explant viability, while 1.5 mg/L was more effective in inducing callus. These findings provide a preliminary basis for optimizing Massoia tissue culture techniques.
Pengaruh Benzil Aminopurin (BAP) terhadap Induksi Tunas dari Nodus anggrek Dendrobium mussauense Media, Media; Noli, Zozy Aneloi
Jurnal Biologi Universitas Andalas Vol 12 No 1 (2024)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.12.1.35-41.2024

Abstract

Dendrobium mussauense has attractive flowers and potential as a parent of a hybrid orchid. The color of this species is cream with violaceous purple venations on the labellum. D. mussauense is an orchid species listed as vulnerable in IUCN. This species is not located in protected areas nor known to be conserved ex-situ. Clonal propagation is an important biotechnological tool for the conservation of orchids. Thin cell layer (TCL) is a micropropagation method using thin-sized explants that facilitate the diffusion of nutrients to tissues. This study examined induction shoot through two explant cutting techniques, namely TCL and non-TCL explant, at various concentrations of BAP. The non-TCL explant was more effective than the TCL explant in inducing the shoot of D. mussauense. The half-strength Murashige and Skoog (MS) medium supplemented with 0.9 mg.L-1 BAP was a suitable concentration to induce the shoot of D. mussauense through non-TCL explant. The average shoot length was 1,8 cm, and the average number of shoots was 5/explant.
Shoot Induction of Dendrobium lasianthera J.J.Smith with Several Types of Cytokinins through In Vitro Culture Arli, Naura Muthiah; Noli, Zozy Aneloi
Jurnal Penelitian Pendidikan IPA Vol 10 No 4 (2024): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i4.5324

Abstract

Dendrobium lasianthera (Orchidaceae) is one of the potential plants as ornamental plants because it has beautiful and long-lasting flowers. Propagation of orchid plants through in vitro culture technique has been widely done to meet market demand. One of the supporting factors in plant propagation through in vitro culture techniques is growth regulators. Cytokinin is one of the growth regulators commonly used in plant propagation, including orchids. The study aims to determine the effect of several cytokinins and find the best type for shoot induction of Dendrobium lasianthera. The method used a Completely Randomized Design (CRD) with 4 treatments and 6 replications with several types of cytokinis (control; BAP 1.2 mg/L, Kinetin 2 mg/L, and Thidiazuron 1 mg/L) in Murashige & Skoog (MS) media. The results showed that several cytokinins significantly affected the number of shoots, time of shoot appearance, and shoot length of D. lasianthera. Thidiazuron was the best cytokinin for in vitro shoot induction of D. lasianthera
Optimalisasi Konsentrasi Thidiazuron untuk Peningkatan Pertumbuhan Protocorm In Vitro Anggrek Grammatophyllum stapeliiflorum Noli, Zozy Aneloi; Arli, Naura Muthiah; Suwirmen
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9067

Abstract

Cytokinins are growth regulators that play a crucial role in cell division and shoot growth. Thidiazuron, a potent cytokinin, was the focus of our research. The research aimed to determine the effect of several concentrations of Thidiazuron on the protocorm growth of Grammatophyllum stapeliiflorum and to identify the best concentration of Thidiazuron (mg/L) for the protocorm growth of G. stapeliflorum orchids in vitro. The research was conducted from January until March 2022 at the Plant Physiology Laboratory, Universitas Andalas, Padang. The research used a Completely Randomized Design (CRD) with 5 treatments and 5 replications. Several concentrations of Thidiazuron were added to MS media with various concentrations: 0 mg L-1 (A), 0.50 mg L-1 (B), 1 mg L-1  (C), 1.50 mg L-1(D), and 2 mg L-1  (E). The parameters in this research were the percentage of life explants, day of shoot appearance, number of shoots, shoot length, number of leaves, number of roots, and root length. The results showed that Thidiazuron significantly affected the protocorm growth of the G. stapeliiflorum orchid compared to the control treatment. Notably, 2 mg L-1  Thidiazuron emerged as the best concentration for all growth parameters of protocorm G. stapeliiflorum, a finding that could significantly impact our understanding of orchid cultivation and plant physiology.
The Effect of Methyl Jasmonate on Massoia Lactone Production in Callus Culture of the Endangered Masoyi Plant (Cryptocarya massoy (Oken) Kosterm.) Aulia, Annisa; Noli, Zozy Aneloi; Idris, Muhammad
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.321-330

Abstract

Cryptocarya massoy (Oken) Kosterm is a plant known for producing essential oils with many applications. However, overexploitation has threatened the sustainability of this plant. This study aims to identify the effect of adding methyl jasmonate to the culture medium on producing secondary metabolites through the callus culture of C. massoy. The methods in this study include callus induction, callus elicitation in liquid culture, callus extraction, analysis of secondary metabolite compounds in C. massoy callus culture, and data analysis. The results show a significant decrease in fresh weight of callus exposed to MeJA stress, depending on the concentration. Regarding secondary metabolites, massoia lactone, the primary compound in C. massoy, was not detected. It is possible that the duration of elicitation was insufficient to induce the biosynthesis and accumulation of detectable levels of massoia lactone. Secondary metabolite production often follows a specific temporal pattern, and certain compounds may require longer exposure times to reach detectable concentrations. The concentration of MeJA influenced the level of other target compound production, where at 0.5 mM MeJA increased the percentage of humulene by 24.64%. MeJA at 1.5 mM enhanced the percentage of ß-copaene (413.11%), ß-ylangene (100%). MeJA at 2 mM increased benzyl benzoate (9.61%). MeJA at 2.5 mM increased α-ylangene (31.25%) and caryophyllene (22.83%). Each MeJA concentration activated specific biosynthesis pathways, influencing the types of secondary metabolites produced. The metabolite screening revealed a predominance of terpenoids, especially sesquiterpenes, which play a role in plant defense.
Manisnya Inovasi Herbal: Pelatihan Pembuatan Permen Jelly Jahe sebagai Camilan Fungsional Lokal di Nagari Batu Kalang Utara Dillasamola, Dwisari; Yufri Aldi; Zozy Aneloi Noli; Uce Lestari; Dehan Fahresta; Abdullah Muhammad Faathir; Aditya Gufashar; Afifah Hulwah; Andhini Aurellyta Ridwan; Fashelia Azizaturrahmah; Habib Shidiq Akbar; Iva Rama Fitria; Larisa Aurellia Vilonia; Lusi Octaviana; Muhammad Fazly; Muhammad Abdul Najib; Muhammad Aidi Satryo; Muhammad Rahil Dief; Nadhira Yuri Maharani; Novita Sari; Saniya De Nafisa; Widya Faizatul Zuhri; Vika Permata Gustam; Yudistio Arnoza; Zainini Arraysa
Jurnal Pengabdian Harapan Ibu (JPHI) Vol. 7 No. 3 (2025): Jurnal Pengabdian Harapan Ibu (JPHI)
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/jphi.v7i3.1126

Abstract

The community service activity aimed to enhance public understanding and skills in utilizing ginger (Zingiber officinale) as a natural ingredient for functional food through the production of jelly candy. This initiative was motivated by the low level of public knowledge, as indicated by pre-test results showing limited understanding of the health benefits and processing techniques of ginger. The activity consisted of educational sessions, demonstrations, and hands-on practice on jelly candy production using natural ingredients, including ginger extract, sugar, jelly powder, vanilla, salt, and cinnamon. Evaluation through post-tests, interactive quizzes, and observation of practical sessions demonstrated a significant improvement in participants’ knowledge and skills. Participants showed enthusiasm and were able to independently produce ginger-based jelly candy. This program not only increased public literacy on the use of herbal ingredients but also encouraged the development of small-scale functional snack enterprises. Future implementations are expected to expand to other communities with continuous mentoring to strengthen economic and health impacts.
Co-Authors . Mansyurdin A. Fadhil Desafa Abdullah Muhammad Faathir Adam Raihan Priambada Adelia, sabbrina Aditya Gufashar Adrial, Rico Afdhal Muttaqin Afifah Hulwah Agusti Apriliani Alimin Mahyudin Alponsin Alponsin Andhini Aurellyta Ridwan Anita Sari Anita Tri Astuti Anthoni Agustien Arif Budiman Arli, Naura Muthiah Asih Maharani Asih, Enda Tarni Asnul Fitria Astuti Aulia Suci Desila Aulia, Annisa Aulya, Nailul Rahmi Ayola Pajrita Ayu Utami Rezki Azharia Khalida Chairul Chairul Chairul Chairul Cleopatra Dahyunir Dahlan De Yudanur, Parissa Anandita Dedi Mardiansyah Dehan Fahresta Delfi, Shyla Aulia Dezi Meutya Dapersal Dian Fitriyani Diana Fadhilah Dilla, Arfa Iskhia Djong Hon Tjong Dola Ratna Yulizar Dwi Pujiastuti Dwi Puryanti Dwisari Dillasamola Efrizal Efrizal Efrizal Efrizal Eka Muliani Elfans Bawalsyah S.A. Elvaswer Elvina Sari Emelta, Citra Endah Mutia Sari Fashelia Azizaturrahmah Feby Zulya Femilya Putri Fera Malta Feriska Handayani Ferry Lismanto Syaiful Feskaharny Alamsjah Fiqi Diyona Fira Julia Putri Fitri, Arya Wisata Gian Wulandari Gita Prima Yudha Habib Shidiq Akbar Haldis Alvaro Hany, Iga Permata Helsya Vellarentika Labukti Hesti Dwi Marcellinna Husri Meli Iga Permata Hany Ilham, Kurniadi Imam Taufiq Iva Rama Fitria Ivo Octaviani Izmiarti Izmiarti, Izmiarti Julita Julita Kiki Ayunda Putri Kurniadi Ilham Larisa Aurellia Vilonia Liza Febriani Sukma Lubis, Amelia Sriwahyuni Lufri Lufri Lusi Octaviana M. Ali Shafii M. Idris M. Teguh Dhiya Ulhaq Mairawita Mairawita, Mairawita Maliza, Rita Marta Linda Marta, Fepi Dwi Mayola Arda Media Media Media, Media Meqorry Yusfi Miftah Mussaumi Adi Mildawati Mildawati Mildawati Millania Putri Shayen Millania Putri Shayen Muhammad Abdul Najib Muhammad Aidi Satryo Muhammad Azwar Muhammad Fazly Muhammad Hanafi Muhammad Idris Muhammad Rahil Dief Nadhira Yuri Maharani Naura Muthiah Arli Nazhira - Fadhilah Nini Firmawati Nopiyanti, Tita Novia Rika Deli Novita Sari Nur, Fauziah Nurhafitri, Amanda Nurwijayanti Olly Norita Tetra Pasha, Gusti Ari Afrilya Periadnadi Periadnadi Prima Fauziah Haq Puspita, Ayumi Rizci Puti Khairunnajwa Amar Putra Santoso Putri Aliyyanti Putri Seti Ayu Putri, Mellanie Alia Putri, Suci Indah Rahma Devi Rahmayati, Riesca Salsabilah Ramacos Fardela Resti Rahayu Reswita Reswita Reza Oktavia Riesca Salsabilah Rahmayati Rilwan Efendi Rismayani, Dessy Rizqa Zidna Chairafahmi Rusiati, Anisa Rahman Saniya De Nafisa Santhyami Santhyami Shayen, Millania Putri Sherin Dien Salsabila Siagian, Marhamah Solfiyeni Solfiyeni Sri Handani Suci Rahmadani Putri Sumaryati Syukur Suwirmen Suwirmen, Suwirmen Syabila, Hutri Dinda Tesri Maideliza Tesri Maideliza Tiara Tiara Tita Nopiyanti Titiek Rukmini Trengginas Eka Putra Tressa Pratywi Gupitasari Uce Lestari Vika Permata Gustam Wenny Rahma Gusni Widya Faizatul Zuhri Yella Prastika Yuda Yossi Eka Saputri Yudistio Arnoza Yufri Aldi Yulianti, Sisi Zainini Arraysa Zulfi Zulkarnain, Alivia