Claim Missing Document
Check
Articles

Kriopreservasi Sperma Manusia: Systematic Review Diana Fadhilah; Adam Raihan Priambada; Zozy Aneloi Noli; Rita Maliza
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 1 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i1.7828

Abstract

This study aims to identify the types of methods and effects of human sperm cryopreservation. This review was written using a literature review method from international databases such as Scopus and PubMed. The results of the research show that by choosing a cryopreservation method, choosing a cryoprotectant agent such as TAT-PRDX2, canthaxanthin, or Holotheria parva coleomic extract, can increase sperm motility, viability and integrity after cryopreservation, the right cryoprotectant agent can defend cells from oxidative stress and fragmentation DNA. Keywords: Cryopreservation, Human, Sperm
PENGARUH PENAMBAHAN PEPTON DALAM MEMPERCEPAT PERKECAMBAHAN Grammatophyllum stapeliiflorum J.J. Smith SECARA IN VITRO Media Media; Zozy Aneloi Noli; Suwirmen Suwirmen
Jurnal Agrotek Tropika Vol 12, No 2 (2024): JURNAL AGROTEK TROPIKA VOL 12, Mei 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i2.6149

Abstract

Grammatophyllum stapeliiflorum termasuk kedalam daftar appendix II CITES. Populasi anggrek ini menurun disebabkan karena aktivitas manusia dan tingkat perkecambahan yang rendah di alam. Teknik kultur jaringan merupakan salah satu solusi perbanyakan anggrek menggunakan biji sebagai sumber eksplan. Pepton merupakan senyawa organik yang bisa meningkatkan perkecambahan dan perkembangan protokorm karena mengandung asam amino, protein biotin, piridoksin, tiamin dan nitrogen. Penelitian ini dilakukan untuk mengetahui pengaruh pepton terhadap perkecambahan biji dan perkembangan protokorm G. stapeliiflorum melalui teknik kultur jaringan. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 4 ulangan. Biji yang sudah matang dari kapsul steril ditaburkan pada media MS tanpa pepton sebagai perlakuan kontrol dan dengan penambahan berbagai konsentrasi pepton (0, 5; 1; 1,5; 2; dan 2,5 g/L). Hasil analisis statistik menunjukkan bahwa pepton memiliki pengaruh yang signifikan dalam mempercepat perkecambahan. Persentase tertinggi perkecambahan biji (88,59%), pembentukan protokorm (61,02%) dan protokorm yang memiliki SAM dan RAM (22,43%) diamati pada media MS yang mengandung 2 g/L pepton.
Uji Antagonis Bacillus subtilis ATTC 6633 dan Trichoderma harzianum terhadap Pertumbuhan Magnaphorte oryzae pada Benih Padi Anak Daro dengan Variasi Lama Perendaman Feskaharny Alamsjah; Zozy Aneloi Noli; Riesca Salsabilah Rahmayati; Suwirmen Suwirmen; Anthoni Agustien; Kurniadi Ilham
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9468

Abstract

Magnaphorte oryzae is a pathogenic fungus that causes blast disease in rice plants. This disease can cause a decrease in rice production. Therefore, it is necessary to control the disease, one of which is by administering microbes that are antagonistic to Magnaphorte oryzae. This research aims to determine the percentage of inhibitory power of Bacillus subtilis ATTC 6633 and Trichoderma harzianum on the growth of Magnaphorte oryzae with varying soaking times of 24, 48 and 72 hours on the Anak Daro rice variety. This research carried out viability tests for Bacillus subtilis ATTC 6633 and Trichoderma harzianum, as well as antagonist tests using the dual culture method. The results showed that the growth inhibition of Magnaphorte oryzae by Bacillus subtilis ATTC 6633 for a soaking period of 24 hours was 28.48% in the weak category, 48 hours was 32.27% and 72 hours was 31.01% in the medium category. Inhibition of Magnaphorte oryzae by Trichoderma harzianum for a soaking period of 24 hours was 50.63%, 48 hours was 57.59%, and 72 hours was 56.32% in the high category.
Pengaruh Lama Perendaman pada Biopriming Padi Anak Daro Menggunakan Bacillus subtilis dan Trichoderma harzianum Zozy Aneloi Noli; Feskaharny Alamsjah; Riesca Salsabilah Rahmayati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10987

Abstract

The majority of Indonesian people consume rice as a staple food. According to data from the Central Statistics Agency (2022), rice production in West Sumatra reached 1,482,996.01 tons in 2019 and continued to decline until 2021, total rice production in West Sumatra was only 1,317,209.38 tons. This is inversely proportional to the population of West Sumatra which increases every year, so efforts need to be made to increase rice production. One of them is the biopriming technique to increase seed vigor. Priming is hydration carried out slowly which aims to ensure that the seed's water potential reaches balance and activates metabolism in the seed before the seed germinates. One of them is biopriming, which is a seed priming technique using biological agents in the form of beneficial microorganisms. Several factors influence the success of the priming technique, one of which is the soaking time. The right soaking time provides a deeper opportunity to absorb water to stimulate the seeds to germinate. This research aims to determine the effect of soaking time on biopriming of the local West Sumatran rice variety "Anak Daro" using Bacillus subtilis and Trichoderma harzianum on the germination of the Anak Daro rice variety. Completely Randomized Design (CRD) factorial pattern with 2 factors with 3 replications. Factor A is a biopriming agent, namely A1 (no microbes), A2 (Bacillus subtilis), and A3 (Trichoderma harzianum). Factor B is the length of soaking, namely B1 (24 hours), B2 (48 hours), and B3 (72 hours). The results showed that Bacillus subtilis and Trichoderma harzianum had a significant effect on maximum growth potential and germination, while soaking time had a significant effect on maximum growth potential and vigor index.
Pengaruh Aplikasi Nanobiostimulan Rumput Laut (Padina minor Yamada) terhadap Kadar Klorofil Kedelai (Glycine max (L.) Merr.) Millania Putri Shayen; Zozy Aneloi Noli; Tesri Maideliza; Suwirmen Suwirmen
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9063

Abstract

Chlorophyll is a green pigment in plants which is important for the process of photosynthesis. The photosynthesis process affects plant growth. Seaweed extract has been proven to be a source of biostimulants that can increase plant growth, one of which is Padina minor. Concentration and frequency are important factors that influence the work of biostimulants. One effort to increase the effectiveness of biostimulants is by changing the crude extract into the form of nanoparticles. This research aims to analyze the effect of concentration, frequency, and interaction between concentration and frequency of crude extract and Padina minor nano extract on chlorophyll levels in soybean plants. The method used was a completely randomized design (CRD) in factorial consisting of 2 factors and 4 replications. Factor A concentration of Padina minor extract, namely control, crude extract 0.4%, nano extract 0.2%, 0.3%, 0.4%, 0.5% and 0.6%. Factor B is the frequency of administration of Padina minor extract, namely 1, 2, and 3 times administration. Based on research that has been carried out, the results showed that administration of 0.4% crude extract increased the average chlorophyll a level by 6.6% and a total of 11%. Meanwhile, chlorophyll b gave the same results as 0.3% nano extract, namely an increase of 14% compared to the control. The total frequency of administration of Padina minor extract was 3 times, and the same as the other treatments, increasing chlorophyll a levels by 4.6%, b by 5.2%, and a total of 4.9% compared to the control. The interaction between concentration and frequency of administration of Padina minor nano extract gives the same results as the crude extract, with lower concentration and less frequency of application in increasing levels of chlorophyll a, chlorophyll b, and total chlorophyll in soybean plants.
Induksi Akar Anggrek Dendrobium lasianthera dengan Pemberian Beberapa Konsentrasi Naphthalene Acetic Acid (NAA) Secara In Vitro Naura Muthiah Arli; Zozy Aneloi Noli
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9289

Abstract

Dendrobium lasinthera is an orchid that has considerable potential as a raw material for anti-cancer drugs and for the production of cut flowers. Therefore, this orchid is a plant with high economic value. This orchid has beautiful and long-lasting flowers. The demand for this orchid is increasing along with its diverse uses, so the propagation of this orchid needs to be increased. In vitro propagation is the most appropriate step to reproduce this orchid. One of the stages that determines the success of orchid propagation through tissue culture is the root induction stage before acclimatization. Using the right type and concentration of Growth Regulatory Substances (ZPT) is very important. This research aims to determine the effect of several NAA Growth Regulatory Substance (ZPT) concentrations and determine the best NAA concentration for Dendrobium lasianthera root induction. This study used a Completely Randomized Design (CRD) with several NAA concentrations (Control (A); 0.5 mg/L (B), 1 mg/L (C), and 1.5 mg/L (D)), on Murashige & Skoog (MS) media. The results of this study show that NAA has a significant influence on the percentage of root formation, root emergence time, and root length in the Dendrobium lasianthera orchid. NAA 1.5 mg/L is the best concentration for induction of Dendrobium lasianthera orchid roots.
Perkecambahan Biji Anggrek Grammatophyllum stapeliiflorum Pada Media MS dengan Penambahan BAP Secara In Vitro Iga Permata Hany; Zozy Aneloi Noli; Suwirmen Suwirmen
Al-Kauniyah: Jurnal Biologi Vol 17, No 1 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i1.27624

Abstract

 AbstrakGrammatophyllum stapeliiflorum merupakan jenis anggrek epifit dengan pertumbuhan vegetatif dan generatif yang relatif lambat. Anggrek ini termasuk ke dalam kelompok CITES Apendiks II. Kultur in vitro merupakan usaha perbanyakan paling efektif untuk tanaman anggrek. Penggunaan media kultur dan Zat Pengatur Tumbuh (ZPT) yang tepat akan meningkatkan keberhasilan perkecambahan biji anggrek secara in vitro. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi media MS dan penambahan BAP terbaik terhadap perkecambahan anggrek G. stapeliiflorum secara in vitro. Penelitian ini menggunakan rancangan acak lengkap dengan 6 perlakuan dan 4 ulangan. Perlakuan berupa variasi konsentrasi media MS dan BAP, yaitu: MS penuh; MS ½ hara makro; MS ¼ hara makro; MS penuh + 1 ppm BAP; MS ½ hara makro + 1 ppm BAP; dan MS ¼ hara makro + 1 ppm BAP. Parameter yang diamati pada penelitian ini, yaitu waktu muncul protokorm dan persentase tahap perkecambahan biji. Data dianalisis menggunakan uji ANOVA dan uji lanjut Duncan New Multiple Range Test dengan taraf 5%. Hasil penelitian menunjukkan bahwa pemberian BAP mampu mempercepat waktu muncul protokorm. Konsentrasi media MS ¼ hara makro + 1 ppm BAP merupakan konsentrasi terbaik untuk perkecambahan biji anggrek tahap 0 hingga tahap 3, sedangkan konsentrasi media MS ¼ hara makro merupakan konsentrasi terbaik untuk mencapai tahap 4 perkecambahan biji anggrek G. stapeliiflorum secara in vitro.AbstractGrammatophyllum stapeliiflorum is a type of epiphytic orchid with relatively slow vegetative and generative growth. This orchid is included in the CITES Appendix II group. In vitro culture is the most effective propagation method for orchid plants. The use of appropriate culture media and growth regulators will increase the success of orchid seed germination in vitro. This study aims to determine the effect of the best concentration of MS media and the addition of BAP on the germination of G. stapeliiflorum orchids in vitro. This study used a completely randomized design with 6 treatments and 4 replications. The treatments consisted of varying concentrations of MS and BAP media, namely: full MS; MS ½ macro nutrients; MS ¼ macro nutrients; full MS + 1 ppm BAP; MS ½ macro nutrients + 1 ppm BAP; and MS ¼ macro nutrients + 1 ppm BAP. The parameters observed in this study were the time when the protocorm appeared and the percentage of seed germination stages. Data were analyzed using the ANOVA test and the Duncan New Multiple Range Test with a level of 5%. The results of the study showed that administration of BAP was able to speed up the time when protocorm appeared. MS media concentration ¼ macro nutrients + 1 ppm BAP is the best concentration for stage 0 to stage 3 orchid seed germination, while MS media concentration ¼ macro nutrients is the best concentration for achieving stage 4 germination of G. stapeliiflorum orchid seeds in vitro. 
Regeneration of Cattleya Amazing Thailand in ½ MS Medium with The Addition of Several Concentration of PGR and Coconut Water Asnul Fitria; Zozy Aneloi Noli; Djong Hon Tjong
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6067

Abstract

One of the Cattleya species with aesthetic and commercial value is Cattleya Amazing Thailand. An efficient protocol with a high rate of plantlet regeneration is through in vitro culture. Plantlet regeneration is affected by the addition of a Plant Growth Regulator (PGR). Auxin and cytokinin (2,4-D and BAP) combined with PGR from organic material such as coconut water can increase the growth of leave and roots in plantlet regeneration. The research aims to determine the best PGRs combination in regenerating the  Cattleya Amazing Thailand orchid in vitro. The research used the Completely Randomized Design (CRD) experimental method for five treatments. The parameter are survival rate, number of leave, and number of roots. Survival rate was analyzed descriptively and both of number of leave and roots were analyzed statistically. All treatments can form 100% plantlets on survival rate parameter. The treatment of 0.1 mg/L 2,4-D + 1 mg/L BAP gave significantly different results in the number of leaf (9.0) and was not significantly different in the number of root. 0.1 mg/L 2,4-D + 1 mg/L BAP is the best combination for in vitro regeneration of the Cattleya Amazing Thailand orchid. 
Antagonist Test of Bacillus subtilis ATTC 6633 and Trichoderma harzianum on the Growth of Magnaphorte oryzae on Several Varieties of Priming Rice Seeds Feskaharny Alamsjah; Zozy Aneloi Noli; Hesti Dwi Marcellinna; Anthoni Agustien; Suwirmen; Kurniadi Ilham
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6112

Abstract

Priming is a seed-soaking technique to increase seed viability and also suppress pathogens' growth. Magnaporthe oryzae is one of the pathogens in rice plants that causes leaf blast disease. The high rice consumption each year in Indonesia is not proportional to the amount of rice plant production, which is affected by the growth of pathogenic fungi. This study aims to determine the viability and percentage inhibition of Bacillus subtilis and Trichoderma harzianum against the growth of the pathogenic fungus Magnaporthe oryzae on local West Sumatra rice varieties Ceredek, Pandan Pulau, and Batang Sungkai. The research was conducted by testing the viability of microbes by counting the number of colonies and testing microbial antagonists with the Dual Culture method on seven days of observation. The results showed that Bacillus subtilis and Trichoderma harzianum could inhibit the growth of the pathogen M. oryzae, which causes leaf blast disease. The viability of Bacillus subtilis after biopriming for 48 hours was most significant on Ceredek variety, which was 15.9x106 cfu/g, and for Trichoderma harzianum on Pandan Pulau variety, which was 0.4x106 cfu/g. The most significant inhibition percentage of Bacillus subtilis and Trichoderma harzianum was obtained in Ceredek rice with a value of 30.13% (medium category) and 63.04% (high category).
PEMBERDAYAAN IBU RUMAH TANGGA DALAM PEMANFAATAN MICROGREEN DAN HEALTHY JUICE UNTUK GIZI KELUARGA Mairawita; Noli, Zozy Aneloi; Alamsjah, Feskaharny; Solfiyeni; Mildawati
Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat Vol. 3 No. 2 (2024): Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jai.v3i2.742

Abstract

Microgreens and healthy juice are closely related to family nutrition and a healthy lifestyle. This community service activity aims to empower homemakers to use microgreen vegetables in the form of kale and kale and make healthy juice from these vegetables to fulfill family nutrition needs as part of efforts to live a healthy lifestyle. Community service activities regarding the introduction of microgreens and healthy juices were held on June 13, 2024, in Limau Manis Selatan Village, Unand Block B lecturer housing complex, Ulugadut, Pauh District, Padang, which was attended by 50 members of Majlis Taklim Darul Ulum. Implementation methods include socialization, discussion, and direct practice. As many as 90% of participants showed increased knowledge about microgreens, and 85% better understood the importance of healthy juice and how to make it. Awareness regarding participants' nutritional knowledge increased, with greater interest in including microgreens and healthy juices in their daily diet. Activities achieved satisfactory results as demonstrated by high participation and enthusiasm during socialization, discussion, and practice sessions. At the end of the activity, interviews were conducted with participants. Live demonstrations and independent practice are proven effective in teaching new knowledge and skills. Challenges such as time and material limitations were overcome with a follow-up plan, including forming small working groups and follow-up sessions to evaluate progress. This activity increased the knowledge, skills, and enthusiasm of Majlis Taklim Darul Ulum members to live a healthy lifestyle. Post-training, the results show the potential for sustainability in encouraging healthy lifestyles of training participants and strengthening communities.
Co-Authors . Mansyurdin A. Fadhil Desafa Adam Raihan Priambada Adelia, sabbrina Adrial, Rico Afdhal Muttaqin Agusti Apriliani Alimin Mahyudin Alponsin Alponsin Anita Sari Anita Tri Astuti Anthoni Agustien Arif Budiman Arli, Naura Muthiah Asih Maharani Asih, Enda Tarni Asnul Fitria Astuti Aulia Suci Desila Aulia, Annisa Aulya, Nailul Rahmi Ayola Pajrita Ayu Utami Rezki Azharia Khalida Chairul Chairul Chairul Chairul Cleopatra Dahyunir Dahlan De Yudanur, Parissa Anandita Dedi Mardiansyah Delfi, Shyla Aulia Della Amelia Dezi Meutya Dapersal Dian Fitriyani Diana Fadhilah Dilla, Arfa Iskhia Djong Hon Tjong Dola Ratna Yulizar Dwi Pujiastuti Dwi Puryanti Efrizal Efrizal Efrizal Efrizal Eka Muliani Elfans Bawalsyah S.A. Elvaswer Elvina Sari Emelta, Citra Endah Mutia Sari Feby Zulya Femilya Putri Fera Malta Feriska Handayani Ferry Lismanto Syaiful Feskaharny Alamsjah Fiqi Diyona Fira Julia Putri Fitri, Arya Wisata Gian Wulandari Gita Prima Yudha Haldis Alvaro Hany, Iga Permata Helsya Vellarentika Labukti Hesti Dwi Marcellinna Husri Meli Iga Permata Hany Ilham, Kurniadi Imam Taufiq Ivo Octaviani Izmiarti Izmiarti, Izmiarti Julita Julita Kiki Ayunda Putri Kurniadi Ilham Liza Febriani Sukma Lubis, Amelia Sriwahyuni Lufri Lufri M. Ali Shafii M. Idris M. Teguh Dhiya Ulhaq Mairawita Mairawita, Mairawita Maliza, Rita Marta Linda Marta, Fepi Dwi Mayola Arda Media Media Media, Media Meqorry Yusfi Miftah Mussaumi Adi Mildawati Mildawati Mildawati Millania Putri Shayen Millania Putri Shayen Muhammad Azwar Muhammad Hanafi Naura Muthiah Arli Nazhira - Fadhilah Nini Firmawati Nopiyanti, Tita Novia Rika Deli Nur, Fauziah Nurhafitri, Amanda Olly Norita Tetra Pasha, Gusti Ari Afrilya Periadnadi Periadnadi Prima Fauziah Haq Puspita, Ayumi Rizci Puti Khairunnajwa Amar Putra Santoso Putri Aliyyanti Putri Seti Ayu Putri, Mellanie Alia Putri, Suci Indah Rahma Devi Rahmayati, Riesca Salsabilah Ramacos Fardela Resti Rahayu Reswita Reswita Reza Oktavia Riesca Salsabilah Rahmayati Rilwan Efendi Rismayani, Dessy Rizqa Zidna Chairafahmi Rusiati, Anisa Rahman Santhyami Santhyami Shayen, Millania Putri Sherin Dien Salsabila Siagian, Marhamah Solfiyeni Solfiyeni Sri Handani Suci Rahmadani Putri Sumaryati Syukur Suwirmen Suwirmen, Suwirmen Syabila, Hutri Dinda Tesri Maideliza Tesri Maideliza Tiara Tiara Tita Nopiyanti Titiek Rukmini Trengginas Eka Putra Tressa Pratywi Gupitasari Wenny Rahma Gusni Yella Prastika Yuda Yossi Eka Saputri Yulianti, Sisi Zulfi Zulkarnain, Alivia