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Optimizing The Effects of Auxin and Cytokinin Combinations on Shoot and Root Induction of Dendrobium mussauense Noli, Zozy Aneloi; Nur, Fauziah; Suwirmen
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6637

Abstract

Dendrobium mussaense, an orchid species of high ornamental value, was subjected to experimental treatments involving the addition of combinations of auxin and cytokinin, namely 2,4-D (2,4-dichlorophenoxyacetic acid) with BAP (6-benzylaminopurine) and 2,4-D with TDZ (thidiazuron). The research aim was to evaluate their effects on the emergence time of shoots and roots. The method used an experiment design with seven treatments. Among the treatments, the most effective was 2,4-D at a concentration of 1 mg/L combined with 0.75 mg/L of BAP. This treatment resulted in the shortest time for both shoot and root emergence. These findings underscore the importance of optimizing hormone combinations for efficient shoot and root induction in D. mussaense, providing valuable insights for orchid propagation and cultivation practices.
Potential of Mesenchymal Stem Cells (MSCs) for Various Disease Therapy: Systematic Review Aulia, Annisa; Rismayani, Dessy; Nopiyanti, Tita; Noli, Zozy Aneloi; Maliza, Rita
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7006

Abstract

Mesenchymal stem cells (MSCs) are a type of multipotent stem cells that can be found in various tissues of the body, possessing good regenerative qualities and promising immunomodulatory abilities for therapy in various medical conditions. MSCs can be obtained from bone marrow, umbilical cord, adipose tissue, and Warthon's jelly. This study aims to present a systematic review of the use of mesenchymal stem cells in disease therapy, by evaluating the latest evidence regarding their efficacy and safety in various therapies. This review uses literature review methods from international data bases such as PubMed and Google Scholar. The total number of articles obtained from the database was 49 articles. These articles were then filtered using inclusion and exclusion criteria, resulting in 22 articles for this systematic review. The research findings indicate that MSCs therapy has been used for the treatment of liver fibrosis, diabetes mellitus, spinal cord injury, and COVID-19. In MSCs therapy, mechanisms involved paracrine effects (MSCs produce cytokines, growth factors, and other proteins); immunomodulation (MSCs can suppress immune cell activation, inhibit lymphocyte proliferation, modulate natural killer T cell activation, and produce anti-inflammatory factors); angiogenesis (MSCs are capable of stimulating the growth of new blood vessels through secretion of VEGF and FGF); cellular differentiation; and anti-fibrotic effects. This is what makes MSCs potentially promising as an evolving alternative treatment.
Effect of Crude Extracts Fern Leaves as Biostimulants on Biomass and Nodulation of Soybean (Glycine max L.) Dilla, Arfa Iskhia; Noli, Zozy Aneloi; Mansyurdin, Mansyurdin
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.8122

Abstract

Soybean (Glycine max) is a primary food commodity for the people in Indonesia, with most of its needs still dependent on imports. Efforts to increase local production require a sustainable strategy, including by use biostimulants. Natural biostimulants, such as fern leaf extract, is proven to be a solution in increasing the growth of plants. This study aims to evaluate the potential use of fern leaf extract as a biostimulant on biomass and the number of root nodules in soybean plants. The method used in this study was a factorial complete randomized design with 2 factors and 3 replications. Factor A is the source of crude extract of fern leaves, control, Gleichenia linearis, Diplazium esculentum, Nephrolepis exaltata, and Blechnum orientale. Factor B is the frequency of application extracts, namely 1 time and 2 times.The results showed that the application of Diplazium esculentum leaf extract with one application significantly increased the total number of root nodules, the number of effective root nodules and vegetative phase biomass. While in Nephrolepis exaltata with one time application can significantly increase the number of total root nodules and the number of effective root nodules. This increase is indicated by the content of bioactive compounds contained in the extract, so that it can increase the growth of soybean plants by stimulating the activity of soil microorganisms.
Effect of Kinetin Concentration on Callus Induction of Cryptocarya massoy (Oken) Kosterm Under in Vitro Conditions Noli, Zozy Aneloi; Hanafi, Muhammad; idris, M.; Hany, Iga Permata
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.8162

Abstract

The Papuan indigenous plant Cryptocarya massoy (Oken) Kosterm has substantial commercial significance as an essential oil source. Plant growth regulators (PGRs) are essential for regulating growth responses including callus development, and tissue culture offers a viable means of reproducing this species. Kinetin, a cytokinin-type PGR, is particularly important in promoting cell division and callus development. This study aimed to evaluate the growth response of C. massoy explants under varying kinetin concentrations and determine the optimal concentration for callus induction. The experiment utilized a Completely Randomized Design (CRD) with three kinetin treatments: 0.5 mg/L (A), 1.0 mg/L (B), and 1.5 mg/L (C). Results showed that 1.0 mg/L kinetin achieved the highest explant survival rate (71%) and the lowest browning rate (8%), compared to 13% browning at 0.5 mg/L and 1.5 mg/L. However, the highest callus formation (0.25 average callus) with a compact, brownish texture was observed at 1.5 mg/L. The 0.5 mg/L concentration consistently exhibited the lowest response across all parameters. These findings indicate that 1.0 mg/L kinetin is optimal for enhancing explant survival and minimizing browning, while 1.5 mg/L is more effective for inducing callus formation, underscoring the importance of kinetin concentration in optimizing C. massoy tissue culture protocols.
Literature Review: The Potential of Phenolic Compounds as Allelopathic Agents in Mangifera indica Noli, Zozy Aneloi; Nurhafitri, Amanda; Puspita, Ayumi Rizci; Putri, Mellanie Alia; Santoso, Putra
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8271

Abstract

Phenolic compounds are a group of secondary metabolites identified as having significant roles in various ecophysiological processes, including allelopathic activity. In Mangifera indica (mango), phenolic compounds contribute to chemical interactions between plants by releasing allelopathic substances that influence the growth and development of surrounding plant species. This study aims to review the potential of phenolic compounds in M. indica as allelopathic agents, focusing on identifying dominant types of phenolic compounds, allelopathic mechanisms, and their ecological impacts on plant communities. The research method employed is a literature review involving a search for relevant sources on Google Scholar covering the period from 2010 to 2024. Based on a review of various studies, it has been demonstrated that phenolic compounds in M. indica possess significant potential to inhibit the growth of competitors and can be utilized in environmentally friendly agricultural management.
Literatur Review: Analysis of Essential Oil Secondary Metabolite Content in Several Plants Noli, Zozy Aneloi; Delfi, Shyla Aulia; Zulkarnain, Alivia; Syabila, Hutri Dinda; Rusiati, Anisa Rahman; Santoso, Putra
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8513

Abstract

Indonesia’s rich plant biodiversity offers a wide array of secondary metabolites, including essential oils, known for their antioxidant, antifungal, and antibacterial properties. This review article examines the secondary metabolite composition of essential oils from various plant species by synthesizing findings from existing literature. The review article highlights the presence of diverse compounds, including terpenes, phenols, and alkaloids, with variations observed between species—for instance, limonin in citrus, linalool in ylang-ylang, and eugenol in cloves. Commonly utilized methodologies, such as steam distillation and GC-MS analysis, discuss their effectiveness in characterizing essential oil components. The findings underscore the extensive potential of crucial oils for applications in health, food, and cosmetic industries, providing a foundation for future research and practical innovations.
Induksi Tunas Anggrek Dendrobium discolor dengan Penambahan Konsentrasi 2,4-D secara In vitro Hany, Iga Permata; Noli, Zozy Aneloi; Idris, M.
AGRITROP Vol 21, No 2 (2023): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v21i2.21095

Abstract

Dendrobium discolor merupakan salah satu anggrek yang memiliki keunikan pada bentuk dan warna bunganya. Bunga yang melengkung ke arah dalam dengan warna kuning keemasan membuat anggrek ini semakin diminati. Kemampuan regenerasi anggrek secara umum sangat lambat jika dibiarkan pada kondisi alamiahnya. Propagasi melalui induksi tunas secara in vitro merupakan usaha perbanyakan yang paling tepat untuk memperbanyak anggrek ini. Penggunaan ZPT 2,4-D mampu menginduksi tunas dengan berperan pada pembelahan sel eksplan D. discolor. Penelitian ini bertujuan untuk melihat pengaruh beberapa konsentrasi ZPT 2,4-D dan menemukan konsentrasi terbaik dalam menginduksi tunas anggrek D. discolor secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan beberapa konsentrasi 2,4-D: A (1 mg/L), B (2 mg/L), C (3 mg/L), dan D (4 mg/L). Hasil penelitian ini menunjukkan bahwa semua perlakuan penambahan 2,4-D dapat memberikan persentase eksplan hidup sebesar 100%. Waktu muncul tunas tercepat ditunjukkan oleh perlakuan 4 mg/L. Propagasi melalui induksi tunas secara in vitro dengan penambahan ZPT 2,4-D sebanyak 4 mg/L merupakan protokol perbanyakan yang optimum untuk anggrek D. discolor.
Pengaruh Aplikasi Nanobiostimulan Rumput Laut (Padina minor Yamada) terhadap Kadar Klorofil Kedelai (Glycine max (L.) Merr.) Shayen, Millania Putri; Noli, Zozy Aneloi; Maideliza, Tesri; Suwirmen, Suwirmen
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9063

Abstract

Chlorophyll is a green pigment in plants which is important for the process of photosynthesis. The photosynthesis process affects plant growth. Seaweed extract has been proven to be a source of biostimulants that can increase plant growth, one of which is Padina minor. Concentration and frequency are important factors that influence the work of biostimulants. One effort to increase the effectiveness of biostimulants is by changing the crude extract into the form of nanoparticles. This research aims to analyze the effect of concentration, frequency, and interaction between concentration and frequency of crude extract and Padina minor nano extract on chlorophyll levels in soybean plants. The method used was a completely randomized design (CRD) in factorial consisting of 2 factors and 4 replications. Factor A concentration of Padina minor extract, namely control, crude extract 0.4%, nano extract 0.2%, 0.3%, 0.4%, 0.5% and 0.6%. Factor B is the frequency of administration of Padina minor extract, namely 1, 2, and 3 times administration. Based on research that has been carried out, the results showed that administration of 0.4% crude extract increased the average chlorophyll a level by 6.6% and a total of 11%. Meanwhile, chlorophyll b gave the same results as 0.3% nano extract, namely an increase of 14% compared to the control. The total frequency of administration of Padina minor extract was 3 times, and the same as the other treatments, increasing chlorophyll a levels by 4.6%, b by 5.2%, and a total of 4.9% compared to the control. The interaction between concentration and frequency of administration of Padina minor nano extract gives the same results as the crude extract, with lower concentration and less frequency of application in increasing levels of chlorophyll a, chlorophyll b, and total chlorophyll in soybean plants.
Induksi Akar Anggrek Dendrobium lasianthera dengan Pemberian Beberapa Konsentrasi Naphthalene Acetic Acid (NAA) Secara In Vitro Arli, Naura Muthiah; Noli, Zozy Aneloi
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9289

Abstract

Dendrobium lasinthera is an orchid that has considerable potential as a raw material for anti-cancer drugs and for the production of cut flowers. Therefore, this orchid is a plant with high economic value. This orchid has beautiful and long-lasting flowers. The demand for this orchid is increasing along with its diverse uses, so the propagation of this orchid needs to be increased. In vitro propagation is the most appropriate step to reproduce this orchid. One of the stages that determines the success of orchid propagation through tissue culture is the root induction stage before acclimatization. Using the right type and concentration of Growth Regulatory Substances (ZPT) is very important. This research aims to determine the effect of several NAA Growth Regulatory Substance (ZPT) concentrations and determine the best NAA concentration for Dendrobium lasianthera root induction. This study used a Completely Randomized Design (CRD) with several NAA concentrations (Control (A); 0.5 mg/L (B), 1 mg/L (C), and 1.5 mg/L (D)), on Murashige & Skoog (MS) media. The results of this study show that NAA has a significant influence on the percentage of root formation, root emergence time, and root length in the Dendrobium lasianthera orchid. NAA 1.5 mg/L is the best concentration for induction of Dendrobium lasianthera orchid roots.
Uji Antagonis Bacillus subtilis ATTC 6633 dan Trichoderma harzianum terhadap Pertumbuhan Magnaphorte oryzae pada Benih Padi Anak Daro dengan Variasi Lama Perendaman Alamsjah, Feskaharny; Noli, Zozy Aneloi; Rahmayati, Riesca Salsabilah; Suwirmen, Suwirmen; Agustien, Anthoni; Ilham, Kurniadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9468

Abstract

Magnaphorte oryzae is a pathogenic fungus that causes blast disease in rice plants. This disease can cause a decrease in rice production. Therefore, it is necessary to control the disease, one of which is by administering microbes that are antagonistic to Magnaphorte oryzae. This research aims to determine the percentage of inhibitory power of Bacillus subtilis ATTC 6633 and Trichoderma harzianum on the growth of Magnaphorte oryzae with varying soaking times of 24, 48 and 72 hours on the Anak Daro rice variety. This research carried out viability tests for Bacillus subtilis ATTC 6633 and Trichoderma harzianum, as well as antagonist tests using the dual culture method. The results showed that the growth inhibition of Magnaphorte oryzae by Bacillus subtilis ATTC 6633 for a soaking period of 24 hours was 28.48% in the weak category, 48 hours was 32.27% and 72 hours was 31.01% in the medium category. Inhibition of Magnaphorte oryzae by Trichoderma harzianum for a soaking period of 24 hours was 50.63%, 48 hours was 57.59%, and 72 hours was 56.32% in the high category.
Co-Authors . Mansyurdin A. Fadhil Desafa Abdullah Muhammad Faathir Adam Raihan Priambada Adelia, sabbrina Aditya Gufashar Adrial, Rico Afdhal Muttaqin Afifah Hulwah Agusti Apriliani Alimin Mahyudin Alponsin Alponsin Andhini Aurellyta Ridwan Anita Sari Anita Tri Astuti Anthoni Agustien Arif Budiman Arli, Naura Muthiah Asih Maharani Asih, Enda Tarni Asnul Fitria Astuti Aulia Suci Desila Aulia, Annisa Aulya, Nailul Rahmi Ayola Pajrita Ayu Utami Rezki Azharia Khalida Chairul Chairul Chairul Chairul Cleopatra Dahyunir Dahlan De Yudanur, Parissa Anandita Dedi Mardiansyah Dehan Fahresta Delfi, Shyla Aulia Dezi Meutya Dapersal Dian Fitriyani Diana Fadhilah Dilla, Arfa Iskhia Dinya Khairani Aisa Tumanggor Djong Hon Tjong Dola Ratna Yulizar Dwi Pujiastuti Dwi Puryanti Dwisari Dillasamola Efrizal Efrizal Efrizal Efrizal Eka Muliani Elfans Bawalsyah S.A. Elvaswer Elvina Sari Emelta, Citra Endah Mutia Sari Fajrisani, Syifa Fashelia Azizaturrahmah Feby Zulya Femilya Putri Fera Malta Feriska Handayani Ferry Lismanto Syaiful Feskaharny Alamsjah Fiqi Diyona Fira Julia Putri Fitri, Arya Wisata Gian Wulandari Gita Prima Yudha Habib Shidiq Akbar Haldis Alvaro Hany, Iga Permata Helsya Vellarentika Labukti Hesti Dwi Marcellinna Husri Meli Iga Permata Hany Ilham, Kurniadi Imam Taufiq Iva Rama Fitria Ivo Octaviani Izmiarti Izmiarti, Izmiarti Julita Julita khairunnisa, kania Kiki Ayunda Putri Kurniadi Ilham Larisa Aurellia Vilonia Liza Febriani Sukma Lubis, Amelia Sriwahyuni Lufri Lufri Lusi Octaviana M. Ali Shafii M. Idris M. Teguh Dhiya Ulhaq Mairawita Mairawita, Mairawita Maliza, Rita Marta Linda Marta, Fepi Dwi Maulana, Zakiy Mayola Arda Media Media Media, Media Meqorry Yusfi Miftah Mussaumi Adi Mildawati Mildawati Mildawati Millania Putri Shayen Millania Putri Shayen Muhammad Abdul Najib Muhammad Aidi Satryo Muhammad Azwar Muhammad Fazly Muhammad Hanafi Muhammad Idris Muhammad Rahil Dief Nadhira Yuri Maharani Naura Muthiah Arli Naura Muthiah Arli Nazhira - Fadhilah Nini Firmawati Nopiyanti, Tita Novia Rika Deli Novita Sari Nur, Fauziah Nurhafitri, Amanda Nurwijayanti Olly Norita Tetra Pasha, Gusti Ari Afrilya Periadnadi Periadnadi Prima Fauziah Haq Puspita, Ayumi Rizci Puti Khairunnajwa Amar Putra Santoso Putri Aliyyanti Putri Seti Ayu Putri, Mellanie Alia Putri, Suci Indah Rahma Devi Rahmayati, Riesca Salsabilah Ramacos Fardela Resti Rahayu Reswita Reswita Reza Oktavia Riesca Salsabilah Rahmayati Rilwan Efendi Rismayani, Dessy Rizqa Zidna Chairafahmi Rusiati, Anisa Rahman Saniya De Nafisa Santhyami Santhyami Sari, Melda Yunita Shayen, Millania Putri Sherin Dien Salsabila Siagian, Marhamah Solfiyeni Solfiyeni Sri Handani Suci Rahmadani Putri Sumaryati Syukur Suwirmen Suwirmen, Suwirmen Syabila, Hutri Dinda Tesri Maideliza Tesri Maideliza Tiara Tiara Tita Nopiyanti Titiek Rukmini Trengginas Eka Putra Tressa Pratywi Gupitasari Uce Lestari Vika Permata Gustam Wenny Rahma Gusni Widya Faizatul Zuhri Yanti, Irda Yella Prastika Yuda Yossi Eka Saputri Yudistio Arnoza Yufri Aldi Yulianti, Sisi Zainini Arraysa Zulfi Zulkarnain, Alivia