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All Journal International Journal of Public Health Science (IJPHS) HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia JOURNAL OF COASTAL DEVELOPMENT Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Kesehatan Lingkungan indonesia Agrointek Indonesian Journal of Educational Review (IJER) Cannarium (Jurnal Ilmu-ilmu Pertanian) Jurnal Riset Industri agriTECH Jurnal Pendidikan Biologi Indonesia Jurnal Industri Hasil Perkebunan Jurnal Penelitian Pendidikan IPA (JPPIPA) Journal of Environment and Sustainability Indonesian Journal of Chemistry Indonesian Journal of Agricultural Research Jurnal Teknologi & Industri Hasil Pertanian Food Science and Technology Journal (Foodscitech) Jambura FIsh Processing Journal Journal of Tropical AgriFood Jurnal Teknologi Hasil Pertanian Jurnal Mutu Pangan : Indonesian Journal of Food Quality Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Riset Teknologi Industri Food ScienTech Journal Journal Of World Science Jurnal Natural Teknologi dan Industri Pertanian Indonesia Jurnal Pertanian Khairun (JPK) Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research) ABDIKU : Jurnal Pengabdian Masyarakat Universitas Mulawarman
Krishna Purnawan Candra
Departement Of Agricultural Poduct Technology, Faculty Of Agriculture, Mulawarman University And Doctoral Study Program Of Environmental Science, Postgraduate Program, Mulawarman University, Jl. Sambaliung, Kampus Gunung Kelua, Samarinda 75119
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Journal : HAYATI Journal of Biosciences

 1 
Solubilization, Activation and Partial Purification of a Sialidase from Horse Liver KRISHNA PURNAWAN CANDRA; PETER ROGGENTIN; ROLAND SCHAUER
HAYATI Journal of Biosciences Vol. 12 No. 3 (2005): September 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.646 KB) | DOI: 10.4308/hjb.12.3.87

Abstract

Using sialyl-methylumbelliferyl -glycoside as substrate, sialidase in horse liver was detected as a membrane-bound enzyme. A yield of about 50% of sialidase activity was found in supernatant when solubilized in 0.1 M sodium-phosphate buffer pH 5.5, containing 0.15 M NaCl, 0.25 M sucrose, and 0.5% Triton X-100. Sialidase in the solubilisate could be activated by incubating in acidic pH at 37 oC. Incubation of this solubilized enzyme at 37 oC for 1.5 h at pH 5.0 led to 10% increase of activity and to the precipitation of about 50% of contaminating protein. Using cation-exchange chromatography on S-Sepharose FF and affinity chromatography on p-aminophenyl oxamic acid-agarose following solubilization and activation, about 6% of total sialidase activity was recovered with the purification factor of about 500. The pH and temperature optimum were measured at pH 4.3 and between 37-45 oC, respectively. Neu5Ac2en was a strong inhibitor, while p-aminophenyl oxamic acid had only a weak inhibitory effect.
Co-Authors Abdul Hakim Agus Setiawan Ainudin Ainudin Akhmad Alberth Parlindungan Alfisyahrani Aloysius Hardoko Andi Syaiful Amin Anis Sofianur Anton Rahmadi Anton Rahmadi Apriadi, Rimbawan Arba Susanty Arboby Gunawan Ariana Ariana Arif Kurniawan Asih Ika Suryandari Aswita Emmawati Bernatal Saragih Bernatal Saragih Bernatal Saragih Bhibies Arghita Nindyana Chandra Kadwa Utama Sutrisno Daniel Tarigan David Patra Didimus Tanah Boleng Elisa Maulidya Putri Elsje Theodora Maasawet Emmawati, Aswita Erna Handai Yani Esti Handayani Hardi Fitriani . Fitriani Fitriani Hadi Suprapto Hamka Haris Retno Susmiyati Hendra Saputra Hendri Wardana Herliani Herliani Hosana, Onedha Hudaida Syahrumsyah Hudaida Syahrumsyah Irma Febriandini Ismail Ismail Iwan Muhamad Ramdan Jayanti, Marni Kasma Kasma Kusdiyanto, Hery Lambang Subagiyo LESTARI, GISTI MALINDA Lisnawati Lisnawati Makrina Tindangen Maria Ulfah Marwati Marwati Masitah - MASRUHIM, MUHAMMAD AMIR Massawet, Elsje Theodore Maulida Rachmawati Miftakhur Rohmah, Miftakhur Muhammad Arifin Muhammad Fahri Muhammad Sultan Muhammad Zulkarnain Ramadana Asir Neni Suswatini Nindyana, Bhibies Arghita Nooryani Nooryani Nur Amaliah Nurul Hidayati Nurul Puspita Palupi Onedha Hosana PETER ROGGENTIN Prabowo, Sulistyo Prabowo, Sulistyo Purwanto, Herry Ratna Setia Ningsih Resi Mandalia Rezeki, Kartika Sri Ria Oktaviani Rinten Anjang Sari Rita Diana ROLAND SCHAUER Sari, Eirine Yemeima Sarinah . Sarwono . Solly Aryza Sukartiningsih Sukartiningsih Sukmiyati Agustin Susanty, Arba Susilo Suwasono, Sony Taufan Purwokusumaning Daru, Taufan Purwokusumaning Tazri Amrullah Tholhah Tholhah Vandalita Rambitan Viky Bayu Parytha WIJAYANTI KUSUMA WARDHANI Wiwit Murdianto Yani, Erna Handai Yeni Yunus Yudha Agus Prayitno Yuliani Yuliani Yuliani, Yuliani Yuliarta, Sri Roma Yusak Hudiyono, Yusak