Article Per Year (5 Year)
p-Index From 2021 - 2026
3.8
P-Index
This Author published in this journals
All Journal Journal of the Indonesian Tropical Animal Agriculture Pembangunan Pedesaan JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Animal Production : Indonesian Journal of Animal Production Bulletin of Applied Animal Research Agrinimal Jurnal Ilmu Ternak dan Tanaman Jurnal Pengabdian Kepada Masyarakat Jurnal Masyarakat Madani Indonesia ANGON: Journal of Animal Science and Technology Jurnal Kedokteran Hewan
Ismoyowati
LPPM - Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

Published : 56 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 56 Documents
Search

 1   2   3   4   5 
Genetic Characteristic of Indonesian Local Ducks Based on Single Nucleotide Polymorphism (SNP) Analysis in D-loop Region Mitochondria DNA Purwantini, Dattadewi; Ismoyowati, Ismoyowati
ANIMAL PRODUCTION Vol 16, No 3 (2014): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (817.306 KB)

Abstract

Abstract. The aim of the study was to know the genetic characteristic and polymorphysm of Indonesian local ducks including Magelang, Tegal, Mojosari, Bali and Alabio duck based on Single Nucleotide Polymorphism (SNP) analysis in D-loop region mtDNA. The long term aim was to set the spesific genetic marker based on SNP D-loop region mtDNA which could differentiate local ducks in Indonesia. In the future, it could be used as selection tool for local duck conservation, and refinement strategy as well as the improvement of genetic quality by utilizing the available native duck germplasm. There were 20 ducks for each duck population and were taken 3 ml of its blood as sample. DNA Isolation Kit high pure PCR template preparation (Geneaid) was uded for Genome DNA isolation.  Amplification with PCR technique used primer DL-AnasPF (L56) as forward and DL-AnasPR (H773) as reverse. Next, PCR product or amplicon were sequenced. Sequence result were analyzed with SNP technique and observed the similarity and difference of its nucleotide sequence between individual and population. The result of the study showed that genome DNA from local duck in Indonesia was successfully isolated. DNA fragment of 718 bp was amplified with primer pair of DL-AnasPF and DL-AnasPR. Nucleotide sequence was 469 nt and analyzed with SNP technique. It was compared with standard nucleotide sequence of Anas platyrhynchos (HM010684.1) in Gen Bank. The result of nucleotide sequence similarity percentage was 99.68±0.56%. Single Nucleotide Polymorphism D-loop region mtDNA Indonesian local duck was 0.32±0.56%.  Some SNP was found in Magelang duck C (Klawu blorok), F (Cemani black),  G (Gambiran), H (Jarakan kalung), I (Jowo plain) and K (Plain white) also Tegal duck 8, 1, 2, 5, 2, 8 and 2 SNP respectively. It could be concluded that polymorphic genetic characteristic similarity were existed in Indonesia local duck populations which was shown by its big standard deviation SNP in D-loop region mtDNA. Magelang duck with different feather color relatively more polymorphic to another local duck in Indonesia. Single Nucleotide Polymorphism which was achieved could be used as genetic marker that differentiate genetic characteristic of Indonesian local ducks.Key words:  genetic characteristic, local duck, Single Nucleotide Polymorphism (SNP), D-loop mtDNAAbstrak.  Penelitian ini bertujuan untuk mengetahui karakteristik genetik dan polimorfisme itik lokal Indonesia yaitu itik Magelang, Tegal, Mojosari, Bali dan Alabio berdasarkan analisis Single Nucleotide Polymorphism (SNP) daerah D-loop mtDNA. Tujuan jangka panjangnya adalah menetapkan marker atau penanda genetik berdasarkan SNP daerah D-loop mtDNA spesifik yang dapat membedakan itik-itik lokal yang ada di Indonesia. Selanjutnya digunakan sebagai  alat bantu seleksi untuk konservasi, pembibitan  dan pengembangbiakan itik lokal.  Populasi masing-masing jenis itik lokal yang digunakan sebanyak 20 ekor untuk diambil 3 ml sampel darahnya. Isolasi DNA genom menggunakan DNA Isolation Kithigh pure PCR template preparation (Geneaid). Amplifikasi dengan teknik PCR menggunakan pasangan primer DL-AnasPF (L56) sebagai forward dan DL-AnasPR (H773) sebagai reverse. Produk PCR atau amplikon yang diperoleh disekuensing. Hasil sekuensing dianalisis dengan teknik SNP dan diamati kesamaan dan perbedaan urutan nukleotida antar individu itik dan antar populasi.  Hasil penelitian menunjukkan bahwa DNA genom dari itik lokal di Indonesia berhasil diisolasi. Amplifikasi dengan teknik PCR berhasil memperoleh fragmen berukuran 718 bp. Urutan nukleotida hasil sekuensing sebesar 469 nt dianalisis dengan teknik SNP dan dibandingkan dengan urutan nukleotida standar dari itik Anas platyrhynchos (HM010684.1) yang ada di Gen Bank, diperoleh persentase kesamaan urutan nukleotid sebesar 99,68±0,56%. Single Nucleotide Polymorphism daerah D-loop mtDNA pada itik lokal di Indonesia sebesar 0,32±0,56%. Sejumlah SNP ditemukan pada itik Magelang C (Klawu blorok), F (Hitam cemani),  G (Gambiran), H (Jarakan kalung), I (Jowo polos) dan K (Putih polos) serta itik Tegal  masing-masing 8, 1, 2, 5, 2, 8 serta 2 SNP. Kesimpulan dari penelitian ini adalah terdapat karakteristik genetik yang polimorfik pada populasi itik lokal di Indonesia, ditunjukkan dengan adanya simpang baku SNP pada daerah D-loop mtDNA yang relatif besar. Itik Magelang dengan warna bulu yang berbeda relatif lebih polimorfik dibandingkan dengan itik lokal lainnya di Indonesia.  Single Nucleotide Polymorphism yang diperoleh dapat digunakan sebagai penanda genetik yang dapat membedakan karakteristik genetik yang dimiliki oleh itik lokal di Indonesia.Kata kunci:  karakteristik genetik, itik lokal, Single NucleotidePolymorphism (SNP),  D-loop mtDNA
Reproduction Performance of Post-Molting Tegal Ducks Given Cattle Reticulum Meal Rosidi, Rosidi; Yuwanta, T; Ismaya, Ismaya; Ismoyowati, Ismoyowati
ANIMAL PRODUCTION Vol 15, No 3 (2013): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.752 KB)

Abstract

Abstract. The objective of this research was to recognize the effect of cattle reticulum meal level as cholesterol source in feed on the quality of post-molting Tegal duck hatching eggs. Experimental method was exercised in this research, using 68-week old Tegal duck consisted of 60 female and 20 male, with cattle reticulum meal treatments (K) namely K0= 0% (control), K1= 1.43% (equaled to 0.371 g cholesterol), K2= 2.86% and K3= 4.29%. Each treatment consisted of three female and one male with 5 time repetition. The observed variables were estrogen level in blood serum, yolk cholesterol, fertility and hatchability. Data were subject to analysis of variance using Completely Randomized Design (CRD), followed by Honestly Significant Difference test (HSD). Result showed that the level of cattle reticulum meal had highly significant effect on fertility, significant effect on estrogen level, and non-significant effect on hatchability and yolk cholesterol. It was concluded that egg fertility could be maintained through the supplementation of cattle reticulum meal up to 2.86%, but it decreased at 4.29%, and that up to 4.29% level of cattle reticulum could not increase egg hatchability. Key words: Fertility, hatchability, Tegal duck, cattle reticulum meal Abstrak. Tujuan penelitian ini adalah untuk mengetahui pengaruh level tepung retikulum sapi sebagai sumber kolesterol yang digunakan dalam  campuran pakan terhadap kualitas telur tetas itik Tegal setelah terjadi molting.  Penelitian menggunakan metode eksperimen.  Materi yang digunakan adalah itik Tegal umur 68 minggu sebanyak 60 ekor induk dan 20 ekor pejantan dengan perlakuan level tepung retikulum sapi dalam pakan (K), terdiri atas K0= 0% (kontrol), K1= 1,43% (setara 0,371 g kolesterol), K2= 2,86% dan K3= 4,29%.  Setiap perlakuan terdiri atas 3 ekor itik induk dan 1 pejantan, yang diulang 5 kali.  Peubah yang diamati adalah kadar hormon estrogen serum darah, kadar kolesterol kuning telur, daya tunas dan daya tetas telur.  Data dianalisis variansi berdasarkan rancangan acak lengkap (RAL), dilanjutkan dengan uji Beda Nyata Jujur (BNJ). Hasil penelitian menunjukkan bahwa level retikulum sapi berpengaruh sangat nyata terhadap daya tunas dan berpengaruh nyata terhadap kadar hormon estrogen, berpengaruh tidak nyata terhadap daya tetas dan kadar kolesterol kuning telur.  Kesimpulannya adalah bahwa daya tunas telur dapat dipertahankan dengan pemberian tepung retikulum sapi sampai dengan level 2,86%, namun menurun pada level 4,29% dan sampai dengan level 4,29% belum mampu meningkatkan daya tetas telur. Kata kunci:  Daya tunas, daya tetas, itik Tegal, tepung retikulum sapi
Detection of Egg Production of Tegal Duck by Blood Protein Polymorphism Ismoyowati, Ismoyowati
ANIMAL PRODUCTION Vol 10, No 2 (2008): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.095 KB)

Abstract

The aim of this research was to study the effect of transfferine, albumine, and haemoglobine loci to egg production characteristic of Tegal duck.  100 lying of Tegal ducks keeping by batteray-pen were used in this study.  Individual egg production was recorded until period of 120 days. Blood protein polymorphism analysed by electrophoresis method, and blood sample taken from each ducks.. Egg production and transfferine albumine, and haemoglobine phenotipe on electrophoresis gel were observed in this study.  Genotipe and gene frequencies and genetic variant were applied in data analysis. The result showed that (1) in the transferine locus were identified 3 aleles forming 4 genotipes (TfAA,TfAB, TfBB, and TfBC), (2) in albumine were identified 3 aleles forming 5 genotipes (AlbAA, AlbAB, AlbAC, AlbBB and AlbBC) and (3) haemoglobine locus were identified 6 aleles forming 4 genotipes ((HbAA, HbAB, HbAC, HbBB, HbBC dan HbCC).  This study demostrated that B gene frequenci in transfferine, albumine and haemoglonine loci was highest than A and C gene frequency.  Tegal Duck with AA genotipe on all loci had higher egg production than BB and CC homozigote.  This research revealed that the most efective of selection method by haemoglobine protein polymorphism. (Animal Production 10(2): 122-128 (2008) Key Words: Tegal duck, egg production, selection, blood protein polymorphism
Maternal Antibody Titer Against Avian Influenza Transferred from Hens to The Eggs and Ducklings Ismoyowati, Ismoyowati; Mufti, M; Samsi, M; Susanto, A
ANIMAL PRODUCTION Vol 15, No 3 (2013): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.862 KB)

Abstract

Abstract.  This research aimed to evaluate the effectiveness of H5N1 Avian Influenza vaccination in different duck breeds and to assess the magnitude of maternal antibody titer transferred from the vaccinated hen to the eggs and off ducklings as a protector agent against H5N1. Experimental research was conducted on 16 male and 48 female mallards and 16 male and 48 female Muscovy ducks aged 16 week old. The study showed that the vaccination was 83.33 % successful in Muscovy ducks and 100% in in mallards. Result of variance analysis demonstrated that breed and sex did not significantly affected AI antibody titer. AI maternal antibody transferred from Muscovy hens to egg yolks and to off springs was 66.37% and 39.51%, respectively. Female Mallards transferred higher antibody to egg yolks than to off springs (96.40% versus 63.18%, respectively. Antibody titer against AI vaccination was determined through ELISA. This study concluded that AI H5N1 vaccination increased antibody titer in ducks which is transferrable to the eggs produced and ducklings. Key words: Vaccination, H5N1 virus, antibody titer, egg yolk, ducklings Abstrak. Penelitian ini bertujuan untuk mengevaluasi efektifitas vaksinasi avian influenza H5N1 yang dilakukan pada bangsa itik yang berbeda. Penelitian juga bertujuan untuk mengetahui besarnya transfer maternal titer antibody dari induk yang divaksinasi ke  telur dan anak itik yang dihasilkan untuk perlidungan terhadap infeksi virus H5N1.  Penelitian dilakukan secara eksperimental dengan materi  menggunakan itik yang terdiri dari itik Tegal jantan 16 ekor dan betina 48 ekor serta entok jantan 16 ekor dan betina 48 ekor umur 16 minggu.  Hasil vaksinasi  menunjukkan keberhasilan vaksinasi sebesar 83.33% pada entok dan pada itik 100%. Hasil analisis variansi  menunjukkan bangsa itik dan jenis kelamin tidak berpengaruh nyata  (P<0.05) terhadap titer antibodi  flu burng (H5N1).  Besarnya transfer maternal antibodi  flu burung dari induk entok  ke kuning telur  sebesar 66,37%, sedangkan pada anaknya 39,51%. Induk itik memberikan antibodi lebih tinggi pada kuning telurnya yaitu sebesar 96,40% dan pada anaknya 63,18%. Kesimpulan dari penelitian ini adalah vaksinasi flu burung H5N1 meningkatkan titer antibodi pada itik yang dapat ditransfer pada telur dan keturunannya. Kata kunci: Vaksinasi, virus H5N1, titer antibodi, kuning telur, anak itik
Supplementation of Vitamin E and C in the Feed on Color, Cooking Loss and Tenderness of Muscovy Ducks Meat Stored in Room Temperature, Refrigerator and Freezer Tugiyanti, Elly; Yuwanta, Tri; Zuprizal, Zuprizal; Rusman, Rusman; Ismoyowati, Ismoyowati
ANIMAL PRODUCTION Vol 17, No 2 (2015): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (577.386 KB) | DOI: 10.20884/1.anprod.2015.17.2.504

Abstract

Research has been conducted to determine the effectiveness of antioxidant supplementation of vitamin E and C in the feed to meat quality of the Muscovy duck meat stored at room temperature, refrigerator and freezer.  Eighty-four Muscovy duck tail males aged 9 weeks maintained for 5 weeks and allocated into 7 treatments with 4 replications and each replication consisted of Muscovy duck 3 heads. Completely Randomized Design was applied, in the which treatments were vitamin E and vitamin C supplementation to the basal feed containing 21% protein and 3100 kcal / kg administered metabolic energy into seven groups namely E0C0: basal feed without Vit E and Vit C, E400: basal feed plus 400 IU of vitamin E, E600: basal feed plus 600 IU of vitamin E, C400: basal feed plus 400 mg / kg feed vitamin C, C600: basal feed plus 600 mg / kg feed as much vitamin C, E200C200: basal feed plus 200 IU vitamin E and 200 mg / kg feed vitamin C, and E300C300: basal feed plus 300 IU of vitamin E and 300 mg / kg feed of vitamin C. The parameters measured were the color of meat that includes the value of L * (lightness), a * (Redness) and b * (yellowness), tenderness and cooking looses.  The data obtained and the analysis of variance followed honestly significant difference test.Muscovy duck meat that vitamin E-supplemented feed brightness levels did not differ (P> 0.05) with non-supplemented,  otherwise Muscovy duck meat that feed supplemented vitamin C or a combination of vitamin E and C levels of brightness up to 3 days either at room temperature or stored the refrigerator is still high.  Supplementation of vitamin E and C was highly significant (P <0.01) against redness (a*) meat stored in different storage means. Muscovy duck meat that feed not supplemented vitamin E and C redness (a*) did not differ (P> 0.05) between the age of 0 hours with that stored in the refrigerator or freezer. B * value of Muscovy duck meat that feed not given vitamin E and C as well as different combinations (P <0.05) with Muscovy duck meat that feed supplemented with vitamin C or a combination of vitamins E and C. The Muscovy duck meat that feed supplemented vitamin E, C or a combination of vitamins E and C if stored at room temperature or refrigerator up to 3 days the value of L* and a* is still high, but the b * low.
Polymorphisme Gene GH and Morphological Characteristic of Anas platyrhynchos and Cairina moschata Febrianto, Firman; Ismoyowati, Ismoyowati; Mufti, Mochamad; Prayitno, Prayitno; Purwantini, Dattadewi
ANIMAL PRODUCTION Vol 20, No 1 (2018)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (609.346 KB) | DOI: 10.20884/1.jap.2018.20.1.665

Abstract

The aims of this research was to identify the qualitative and quantitative difference of the phenotypes of native mallard and Muscovy, and Muscovy the genetic diversity between Muscovy and mallard using RFLP method with growth hormone primer (GH) gene. The materials were 30-week old male and female ducks from 5 strains—Magelang, Mojosari and Tegal ducks, white-feathered, and black and white-feathered Muscovy. Muscovy Completely Randomized Design was used to analyze parameters 5 strains of ducks based on gender and with 5 replications. The parameters measured were local and Muscovy duck phenotypes and genetic diversity (number of alleles) based on GH gene, heterozygosity and genetic distance. Qualitative properties were analyzed descriptively and quantitative properties were analyzed using Anova followed by HSD in case of significant differences, whereas RFLP analysis was used to determine the allele frequencies, genotype frequencies, genetic diversity, and genetic distance of local ducks. Result showed phenotypic differences between Anas platyrhynchos and Cairina moschata. The dominant feather colorof Muscovy was black and white while mallard was brown. The bill color Muscovy Muscovy was pink with dark brown but black in mallard. The dominant shank’s color in both Muscovy and mallard was black. Body size of mallard was larger than that of Muscovy Muscovy, and Magelang ducks weigherd more than Tegal and Mojosari Duck. The PCR-RFLP results showed lower heterozygosity of mallard compared to Muscovy based on GH gene, and genetic distance of the Tegal duck was closer to Mojosari and Magelang ducks, whereas Magelang and Mojosari ducks had a considerable genetic distance based on the GH gene.
THE EFFECT OF STRAIN AND ALTITUDE ON BROILER PERFORMANCE Rosidi, Rosidi; Suswoyo, Imam; Tugiyanti, Elly; Ismoyowati, Ismoyowati
ANIMAL PRODUCTION Vol 1, No 2 (1999): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.922 KB)

Abstract

The research aimed to study the effect of strain and altitude on broiler performance, and to find out suitable strains kept either on low and high altitude. Low altitude was in Purwonegoro North Purwokerto (80 m asl) and high altitude was in Munggangsari Baturaden (755 m asl). The research was carried out since September 5th to December 5th, 1996 using 2x3 Factorial based on Completely Randomized Design. Factor I were land altitude (d) : low (d1) and high (d2). Factor II were broiler strains (g) : CP-707 (g1), AS-101 (g2) and Lohmann (g3). Data obtained were analyzed using F test continued  to Least Significant Difference Test. The research indicated that interaction between altitude and strains significantly affected  feed convertion and income over feed  and doc cost, while strain had significant effect on feed convertion. AS-101 and Lohmann strains  kept on low altitude had the highest final weight. The low altitude gave better feed convertion and income over feed and doc cost, whereas CP-707 strain had the best feed convertion. (Animal Production 1(2): 82-89 (1999) Key Words: strain, altitude, performance, broiler
THE EFFICACIES OF BANANA STEM EXTRACT AS A CANDIDATE OF COCCIDIOSTAT AGAINST RABBIT EIMERIA STIEDAIO OCYSTS: AN IN VITRO ANALYSIS Indrasanti, Diana; Indradji, Mohandas; Hastuti, Sri; Wihadmadyatami, Hevi; Ismoyowati, Ismoyowati
ANIMAL PRODUCTION Vol 17, No 3 (2015): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (741.083 KB) | DOI: 10.20884/1.anprod.2015.17.3.503

Abstract

The objective of this research was to investigatethe ability of banana stem (Musa paradisiaca) to inhibitsporulation of Eimeria stiedaioocystsderived fromrabbit by in vitroanalysis.Analyze the active substance proximate analysis and active substancesin this research were performed too. Banana stem extract were used in this experiment andsulfaquinoxalline(Coxy ®)was run as acontrol. The Eimeria stiedaioocystswere incubated prior the presence of  different concentration from banana stem extract  0%, 1%, 2%, 4%, 8%for 1, 2 and 3 daysat 26°C. In addition,Factorial patterned Completely Randomized Design (CRD) with five replicates wasapplied on the experiment. Result analysis was performed by using Analysis of Variance and following by Honestly Significant Difference (HSD) post hoc test. Here, we identified that banana stem extract contain different type of active substance such as tannin, saponin, and alkaloid. Banana stem extract significantly affected the oocysts sporulation included the amount of sporulatedoocysts (P<0.01), unsporulatedoocysts (P<0.01), and transformed oocysts (P<0.01). In conclusion banana stem could inhibit the development of Eimeria stiedaioocysts on in vitroexperiment. HSD test showed that the optimum potential efficacy of banana stem toinhibit sporulation was at 4% and 8% concentration during three days incubation.
Performative and economic analysis on local duck farming in Central Java - Indonesia Ismoyowati, I.; Pratama, B. C.; Innayah, M. N.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 3 (2020): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.3.234-242

Abstract

Central Java is the region that produces the second largest duck egg production in Indonesia or reaches 13.55% of the total national duck egg production. Therefore, duck farming in Central Java requires an economic evaluation to identify the success of the business. The economic analysis in the duck farm industry will update the latest statistics on production costs, outputs and profitability. This study aims to assess the analysis of laying duck business, which includes: marginal production costs, total net income and input-output ratio of duck egg production in various regions in Central Java, Indonesia. The study was conducted using a survey method using multi-stage sampling involving 120 duck farmers from the regency of Tegal, Brebes, and Pemalang. Each district was taken 40 farms as respondents by purposive random sampling with criteria for the number of breeders who had a minimum duck population of 100 ducks and a production period of 8-12 months. The analytical tools used include descriptive statistics and farm budgetary analysis. This study showed that duck breeders incur large costs to obtain production inputs, especially from feed aspects. Although three regencies reported profit based on the estimated input-output ratios, it is only a marginal profit that amounted to1: 1.382, 1:1.658, and 1:1.433 in Tegal, Brebes, and Pemalang, respectively. The conclusion of the study is the performance of duck farms in Central Java are still profitable even in the smallholder duck farm industry, with the highest profits obtained by farmers in Brebes Regency. 
Morphometrics and genetic diversity of Tegal, Magelang and their crossbred ducks based on Cytochrome b gene Henrik, H.; Purwantini, D.; Ismoyowati, I.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 1 (2018): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.1.9-18

Abstract

This experiment was conducted to study the morphometric and genetic diversity of Tegal, Magelang, and their crossbred ducks. Each ten female about twenty weeks old ducks from Tegal, Magelang, Gallang, and Maggal ducks, respectively, were used as the group materialss. The body weight, chest circumference, body length, shank length, neck length, and pubis width were recorded and tested by analysis of variance of one way classification as the morphometric characteristics. Polymorphism of cytochrome b (cyt b) gene on mitochondrial DNA (mtDNA) was analyzed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Genetic distance was analyzed based on value of heterozygosity, whereas the phylogeny tree was reconstructed using MEGA6 software. The results showed there were highly significant difference (P<0.01) on body weight, chest circumference, body length, and neck length between population, while shank and pubis width were not significant different. The genetic distance between Gallang and Maggal ducks (0.206) was higher than Tegal and Magelang ducks (0.169). It is concluded that the reciprocal crosses increased the morphometric and genetic diversity of Indonesian local duck population.
Co-Authors A Susanto A. Santosa A.A. Ketut Agung Cahyawan W Abdoreza Soleimani Farjam, Abdoreza Soleimani Achmad Iqbal Achmad Shawaludin Ali Akbar, Rizka Faizul Albasheer, Modawy Abdelgader Andri Setianto, Novie Any Kurniawati, Any Armelia, Vony ATA Sudewo, ATA Bambang Hartoyo D. Purwantini Dadang Mulyadi Saleh Dattadewi Purwantini Dattadewi Purwantini Diana Indrasanti Dyahruri Sanjayasari E. Tugiyanti Efka Aris Rimbawanto Eka Lestari Elly Tugiyanti Evri Kalis Untari Febrianto, Firman Fita Septiana Sari Hadi, Kemal Hazza Fadhillah Harnowo Sidhi, Arif Hasanah, Lailattun Henrik Henrik Heratri, Agnes Hevi Wihadmadyatami Hidayat, Nu'man Hidayat, Nu'man - I Suswoyo, I Ibnu Hari Sulistyawan Ika Rostika Imam Suswoyo Imam Suswoyo Imron Rosadi Innayah, M. N. Ismaya Ismaya J HP Sidadolog, J HP Japutra, Bambang Rijanto Khoirudin, Nandiwahib Mualim Kusuma, Selfia Anggraini Lupita, Silvia Ade M Mufti, M M Samsi, M Maulidya Siella Ningtyas Maulidya, Nur Aini Mochamad Mufti Mohandas Indradji Muflihah, Ganis Mufti, M. Muhamad Samsi Muhammad Rayhan Mustika, Tisha Bunga N. Hidayat, N. N. Iriyanti Nena Kharisma Armissaputri Nikmatul Arifah Ning Iriyanti Novie Andri Setianto Novita Hindratiningrum Nugroho, Aras Prasetyo Nur Hidayanto Nurani, Uci Pratama, B. C. Prayitno Prayitno Rahayu Sri Pamungkas Ramadhani, Mega Destri Ratna Stia Dewi Refriana, Rosita Rijanto Japutra, Bambang Roesdiyanto Roesdiyanto Rosidi Rosidi Rosidi, R. Rusman Rusman S Keman, S S. A. Santoso, S. A. SA Santosa, SA Setya Agus Santosa Sigit Mugiyono Siti Hilda A. Lestari Sobryan, Arief Solly Aryza Sukardi Sukardi Sukardi Sulistiyono, Ibnu Hari T Yuwanta T. Hartatik T. Yuwanta Titin Widiyastuti Tri Yuwanta Veithzal Rivai Zainal Vian Graha Wijaya Wardaya, Ziya Auliya Yakubu, M. A. Yulfikar Ismoyojati Yulia Wasnaeni, Yulia Zuprizal (Zuprizal)